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Pseudomonas aeruginosa synergies

Seunggyu Kim, Fahim Masum, Ju-Kang Kim, Hyun Jung Chung, Jessie S Jeon
Combinatory therapy using two or more kinds of antibiotics is attracting considerable attention for inhibiting multi-drug resistant pathogenic bacteria. Although the therapy mostly leads to more powerful antimicrobial effects than using a single antibiotic (synergy), interference may arise from certain antibiotic combinations, resulting in the antimicrobial effect being suppressed (antagonism). Here, we present a microfluidic-based phenotypic screening chip to investigate combinatory antibiotic effects by automatically generating two orthogonal concentration gradients on a bacteria-trapping agarose gel...
February 15, 2019: Lab on a Chip
Priyadarshini Singha, Christina D Workman, Jitendra Pant, Sean P Hopkins, Hitesh Handa
The development of infection resistant materials is of substantial importance as seen with an increase in antibiotic resistance. In this project, the nitric oxide (NO)-releasing polymer has an added topcoat of zinc oxide nanoparticle (ZnO-NP) to improve NO-release and match the endogenous NO flux (0.5 - 4 x 10-10 mol cm-2 min-1 ). The ZnO-NP is incorporated to act as a catalyst and provide the additional benefit of acting synergistically with NO as an antimicrobial agent. The ZnO-NP topcoat is applied on a polycarbonate-based polyurethane (CarboSil) that contains blended NO donor, S-nitroso-N-acetylpenicillamine (SNAP)...
February 8, 2019: Journal of Biomedical Materials Research. Part A
Fengfeng Xiao, Bing Cao, Congyu Wang, Xujuan Guo, Mengge Li, Da Xing, Xianglong Hu
Highly pathogenic Gram-negative bacteria and the drug resistance are severe public health threat with high mortality. Gram-negative bacteria are hard to kill due to the complex cell envelopes with low permeability and extra defensive mechanisms. It is challenging to treat them by current strategies, mainly including antibiotics, peptides, polymers, and some hybrid materials, still facing the issue of drug resistance, limited antibacterial selectivity, and severe side effects. Together with precise bacteria targeting, synergistic therapeutic modalities including physical membrane damage and photodynamic eradication are promising to combat Gram-negative bacteria...
January 11, 2019: ACS Nano
Oscar Cirioni, Oriana Simonetti, Gianluca Morroni, Lucia Brescini, Wojciech Kamysz, Elzbieta Kamysz, Fiorenza Orlando, Elisa Pierpaoli, Miriam Caffarini, Monia Orciani, Claudio Agostinelli, Annamaria Offidani, Mauro Provinciali, Andrea Giacometti
BACKGROUND: Pseudomonas aeruginosa is a gram-negative pathogen, related with a severe mortality rate. It is also difficult to treat due to numerous resistance mechanisms to a wide range of antibiotics. OBJECTIVE: Evaluate the activity of pexiganan, an antimicrobial peptide, in combination with two clinical antibiotics (azithromycin and tigecycline) that are not active against P. aeruginosa. METHOD: Ten clinical P. aeruginosa were isolated from urinary tract infections, blood culture, skin infections and respiratory tract infections...
December 19, 2018: Current Topics in Medicinal Chemistry
Maytham Hussein, Mei-Ling Han, Yan Zhu, Elena K Schneider-Futschik, Xiaohan Hu, Qi Tony Zhou, Yu-Wei Lin, Dovile Anderson, Darren J Creek, Daniel Hoyer, Jian Li, Tony Velkov
Polymyxins are amongst the most important antibiotics in modern medicine, in recent times their clinical utility has been overshadowed by nosocomial outbreaks of polymyxin resistant MDR Gram-negative 'superbugs'. An effective strategy to surmount polymyxin resistance is combination therapy with FDA-approved non-antibiotic drugs. Herein we used untargeted metabolomics to investigate the mechanism(s) of synergy between polymyxin B and the selective estrogen receptor modulator (SERM) tamoxifen against a polymyxin-resistant MDR cystic fibrosis (CF) Pseudomonas aeruginosa FADDI-PA006 isolate (polymyxin B MIC=8 mg/L , it is an MDR polymyxin resistant P...
2018: Computational and Structural Biotechnology Journal
M N Tarana, S M Shamsuzzaman
Pathogenic microorganisms are important cause of maternal and neonatal infections which are transmitted from colonized vagina of mother. The purpose of the present study was to detect the potential pathogens other than Group B Streptococcus in vaginal swab of pregnant women. This prospective cross sectional study was conducted from July 2013 to June 2014 at Dhaka Medical College Hospital, Dhaka, Bangladesh. A total of 224 vaginal swab samples were studied. Gram stain Nugent score was applied for all vaginal smear to detect bacterial vaginosis...
October 2018: Mymensingh Medical Journal: MMJ
Muhammad Ibrahim Rashid, Amjad Ali, Saadia Andleeb
Dual Oxidase 1 (DUOX1) is a prominent immune system component primarily expressed in esophagus, lungs, skin, and urinary bladder including others. DUOX1 is involved in lactoperoxidase-mediated innate immunity at mucosal surfaces by generation of antimicrobial hypothiocyanite at the apical surface of epithelial lining. Upon detection of bacterial pathogens mainly Pseudomonas aeruginosa, DUOX1 is activated in bronchial epithelial cells. Both the host and pathogen enter a redox dual with DUOX1 and hypothiocyanite from host and Pyocyanin (PCN) as a redox active virulence factor from P...
November 27, 2018: Interdisciplinary Sciences, Computational Life Sciences
Bruno Casciaro, Maria Rosa Loffredo, Vincenzo Luca, Walter Verrusio, Mauro Cacciafesta, Maria Luisa Mangoni
BACKGROUND: The massive use of antibiotics has led to the selection of resistant bacterial strains that are difficult to eradicate. Among these, Pseudomonas aeruginosa most frequently colonizes and infects the airways of cystic fibrosis patients. Cationic Antimicrobial Peptides (AMPs) represent interesting molecules for the development of new antimicrobial agents. Thanks to their mechanism of action that involves the permeabilization of the bacterial cytoplasmic membrane, the induction of resistance is quite limited...
October 31, 2018: Protein and Peptide Letters
Lauro Vieira Perdigão Neto, Maura S Oliveira, Roberta Cristina Ruedas Martins, Ana Paula Marchi, Juliana Januário Gaudereto, Lucianna Auxi Teixeira Josino da Costa, Lia Fernandes Alves de Lima, Christianne Fernandes Valente Takeda, Silvia F Costa, Anna S Levin
Background: In vitro and clinical studies using parenteral fosfomycin have suggested the possibility of using this drug against infections caused by MDR microorganisms. The aim of this study was to describe a case series of patients treated with fosfomycin who had severe infections caused by pan-drug-resistant Gram-negative bacteria. Methods: We describe a prospective series of cases of hospitalized patients with infections caused by Gram-negative bacteria resistant to β-lactams and colistin, treated with 16 g of fosfomycin daily for 10-14 days...
October 29, 2018: Journal of Antimicrobial Chemotherapy
Sara Mombini, Seyedeh Elham Rezatofighi, Laleh Kiyani, Hossein Motamedi
The microbiological quality of drinking water has long been a critical element in public health. Considering the high clinical relevance of Pseudomonas aeruginosa, we examined the filters of household water treatment systems for its presence and characteristics to determine the systems' efficiency in eliminating the bacteria. In total, filters of 50 household water treatment systems were examined. Microbiological and molecular methods were used for the detection and confirmation of P. aeruginosa isolates. Random Amplification of Polymorphic DNA-polymerase chain reaction (RAPD-PCR) was performed to detect similarities and differences among P...
October 24, 2018: Journal of Environmental Management
X Zhang, L Wang, H Mu, D Wang, Y Yu
Some plants of Buddleja genus possessed antimicrobial activity. In this study, the antibacterial activities of nine compounds from B. albiflora were evaluated against Listeria monocytogenes, Staphyloccocus aureus, Escherichia coli and Pseudomonas aeruginosa, either alone or in combination with Erythromycin (Ery), Gentamycin (Gen) and Ampicillin (Amp). Checkerboard assay demonstrated that kumatakenin (Kum) elicited synergistic effects with all three antibiotics, and chrysin (Chr) displayed synergistic effect with Ery against L...
October 9, 2018: Letters in Applied Microbiology
Wonhee So, James Shurko, Ralph Galega, Rod Quilitz, John N Greene, Grace C Lee
No abstract text is available yet for this article.
October 8, 2018: Journal of Antimicrobial Chemotherapy
Yu Lin, Rachel Yoon Kyung Chang, Warwick J Britton, Sandra Morales, Elizabeth Kutter, Hak-Kim Chan
Nebulization is currently used for delivery of antibiotics for respiratory infections. Bacteriophages (or phages) are effective predators of pathogens including Pseudomonas aeruginosa commonly found in the lungs of patients with cystic fibrosis (CF). It is known that phages and antibiotics can potentially show synergistic antimicrobial effect on bacterial killing. In the present study, we investigated synergistic antimicrobial effect of phage PEV20 with five different antibiotics against three P. aeruginosa strains isolated from sputum of CF patients...
November 15, 2018: International Journal of Pharmaceutics
Hadis Tavafi, Ahya Abdi Ali, Parinaz Ghadam, Sara Gharavi
BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen and utilizes several virulence factors for pathogenesis. One of the most important factors is alginate, found in the biofilm which enables P. aeruginosa to establish chronic lung infections. MATERIALS AND METHODS: In this study, 25 clinical alginate-degrading isolates were selected. Biochemical and molecular approach were carried out to identify the isolates by 16S rDNA gene amplification. Growth conditions and enzyme production were the criteria for selection...
August 15, 2018: Microbial Pathogenesis
Chulamartd Wirapakorn, Uthaibhorn Singkham-In, Sujittra Taratummarat, Saowapha Surawut, Peerapat Visitchanakun, Tanittha Chatsuwan, Asada Leelahavanichkul
BACKGROUND: Ascorbate is a low-cost compound with a known bactericidal-synergy to antibitics. However, the synergy depends on concentrations and organisms. Thus, the synergy test by time-kill assay might be appropriate for the screening of the synergy. OBJECTIVE: We aimed to test the adjuvant property of ascorbate with ceftriaxone, a frequently prescribed β-lactam antibiotic. METHOD: Ascorbate was tested with several bacteria from the American Type Culture Collection (ATCC) including Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli for i) bactericidal property of ascorbate, alone or with ceftriaxone-combination, by time-kill assay, ii) an influence on the killing-activity of bone -marrow-derived macrophage and iii) the attenuation of myositis mouse model...
August 13, 2018: Asian Pacific Journal of Allergy and Immunology
Vanessa E Rees, Rajbharan Yadav, Kate E Rogers, Jürgen B Bulitta, Veronika Wirth, Antonio Oliver, John D Boyce, Anton Y Peleg, Roger L Nation, Cornelia B Landersdorfer
Hypermutable Pseudomonas aeruginosa organisms are prevalent in chronic respiratory infections and have been associated with reduced lung function in cystic fibrosis (CF); these isolates can become resistant to all antibiotics in monotherapy. This study aimed to evaluate the time course of bacterial killing and resistance of meropenem and ciprofloxacin in combination against hypermutable and nonhypermutable P. aeruginosa Static concentration time-kill experiments over 72 h assessed meropenem and ciprofloxacin in mono- and combination therapies against PAO1 (nonhypermutable), PAOΔ mutS (hypermutable), and hypermutable isolates CW8, CW35, and CW44 obtained from CF patients with chronic respiratory infections...
November 2018: Antimicrobial Agents and Chemotherapy
Jane C Davies, Isaac Martin
Cystic fibrosis is characterized by bacterial lung infection, a majority of adults being chronically infected with Pseudomonas aeruginosa. Treatment is a major challenge, with frequent courses of antibiotics contributing to antimicrobial resistance. New approaches are clearly required. Over the last few years, a major shift in our approach to treating CF has occurred with the availability of the first drugs targeting the CFTR protein and leading to improvements in lung function, weight gain and frequency of exacerbations...
August 2018: Expert Opinion on Pharmacotherapy
Daisuke Sakanashi, Narimi Miyazaki, Yuzuka Kawamoto, Tomoko Ohno, Atsuko Yamada, Isao Koita, Setsuo Miyajima, Hiroyuki Suematsu, Mao Hagihara, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
We determined the optimal antimicrobial in the sodium mercaptoacetic acid double disk synergy test (SMA-DDST) for the detection of IMP-1-producing Pseudomonas aeruginosa isolates in Japan and evaluated the performance of the test. Fifty-four P. aeruginosa clinical isolates were tested, including 39 IMP-1 producers and 15 non-metallo-β-lactamase (MBL)-producing carbapenem- and ceftazidime (CAZ)-resistant isolates. The SMA-DDST was performed with CAZ, cefepime (CFPM), imipenem (IPM), meropenem (MEPM), doripenem (DRPM), or biapenem (BIPM)-containing disks...
August 9, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Tonny Loho, Ninik Sukartini, Dalima A W Astrawinata, Suzanna Immanuel, Diana Aulia, Ika Priatni
Evaluation of the in vitro interaction of doripenem and amikacin against Acinetobacter baumannii , Pseudomonas aeruginosa , and Klebsiella pneumoniae was done by classifying them into four groups: doripenem and amikacin sensitive (DOR-S/AMK-S), doripenem sensitive and amikacin resistant (DOR-S/AMK-R), doripenem resistant and amikacin sensitive (DOR-R/AMK-S), and both doripenem and amikacin resistant (DOR-R/AMK-R). The MIC of each antibiotic and their combination was obtained using the Etest method. The fractional inhibitory concentration index was calculated to classify the results as synergistic, additive, indifferent, or antagonistic interaction...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
Ana Rita Brochado, Anja Telzerow, Jacob Bobonis, Manuel Banzhaf, André Mateus, Joel Selkrig, Emily Huth, Stefan Bassler, Jordi Zamarreño Beas, Matylda Zietek, Natalie Ng, Sunniva Foerster, Benjamin Ezraty, Béatrice Py, Frédéric Barras, Mikhail M Savitski, Peer Bork, Stephan Göttig, Athanasios Typas
The spread of antimicrobial resistance has become a serious public health concern, making once-treatable diseases deadly again and undermining the achievements of modern medicine1,2 . Drug combinations can help to fight multi-drug-resistant bacterial infections, yet they are largely unexplored and rarely used in clinics. Here we profile almost 3,000 dose-resolved combinations of antibiotics, human-targeted drugs and food additives in six strains from three Gram-negative pathogens-Escherichia coli, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa-to identify general principles for antibacterial drug combinations and understand their potential...
July 2018: Nature
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