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Endometrial disease

Sara J Zakem, Tyler P Robin, Derek E Smith, Arya Amini, William A Stokes, Carolyn Lefkowits, Christine M Fisher
OBJECTIVE: Gynecologic oncology group protocol 249 (GOG 249) is the contemporary US study that aimed to define the standard of care adjuvant therapy for patients with high-intermediate risk (HIR) endometrial cancer; patients were randomized to pelvic radiation therapy (RT) or vaginal brachytherapy (VBT) with chemotherapy (VBT-C). The preliminary results of GOG 249 were recently presented, yet the management of patients represented in this trial remains controversial. We set out to review US patterns of care for patients meeting eligibility criteria for GOG 249...
March 2019: Gynecologic Oncology
Themistoklis Mikos, Dimitrios Tsolakidis, Grigoris F Grimbizis
The aim of this study is to investigate clinical behavior of Atypical Polypoid Adenomyomas (APAs) and to describe the rates of (i) recurrences, (ii) their association with endometrial hyperplasia and (iii) with endometrial cancer. All studies that reported the outcome of the clinical management of patients with histologically proven APAs were included. A review of the English literature since 1970 was systematically performed (PROSPERO No CRD42018080003). A quality assessment tool was used to assess the scientific value of all the studies...
March 2, 2019: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Sally Mortlock, Restuadi Restuadi, Rupert Levien, Jane E Girling, Sarah J Holdsworth-Carson, Martin Healey, Zhihong Zhu, Ting Qi, Yang Wu, Samuel W Lukowski, Peter A W Rogers, Jian Yang, Allan F McRae, Jenny N Fung, Grant W Montgomery
BACKGROUND: Major challenges in understanding the functional consequences of genetic risk factors for human disease are which tissues and cell types are affected and the limited availability of suitable tissue. The aim of this study was to evaluate tissue-specific genotype-epigenetic characteristics in DNA samples from both endometrium and blood collected from women at different stages of the menstrual cycle and relate results to genetic risk factors for reproductive traits and diseases...
March 14, 2019: Clinical Epigenetics
Tomoko Hirakawa, Kaei Nasu, Yoko Aoyagi, Kanetoshi Takebayashi, Ruofei Zhu, Hisashi Narahara
A number of genes involved in the pathogenesis of endometriosis are silenced by the hypermethylation of their promoter regions. We assessed the effect and mechanism of the DNA demethylating agent 5-aza-2'-deoxycytidine (5-aza-dC) (10 μM) on the cell cycle in human endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) by flow cytometry. The DNA methylation status of G2/M checkpoint regulators were investigated by methylation-specific polymerase chain reaction (PCR). The expression of ATM and the effect of 5-aza-dC on its expression were also evaluated by quantitative reverse transcription-PCR and western blotting analysis...
March 14, 2019: Molecular Human Reproduction
Aminah Ali Abid Al-Juboori, Arnab Ghosh, M Fairuz B Jamaluddin, Manish Kumar, Subhransu S Sahoo, Shafiq M Syed, Pravin Nahar, Pradeep S Tanwar
Epithelial and stromal communications are essential for normal uterine functions and their dysregulation contributes to the pathogenesis of many diseases including infertility, endometriosis, and cancer. Although many studies have highlighted the advantages of culturing cells in three-dimensional (3D) compared to the conventional two-dimensional (2D) culture system, one of the major limitations of these systems is the lack of incorporation of cells from non-epithelial lineages. In an effort to develop a culture system incorporating both stromal and epithelial cells, we developed 3D endometrial cancer spheroids by co-culturing endometrial stromal cells with cancerous epithelial cells...
March 13, 2019: Proteomics
Erin A Salinas, Marina D Miller, Andreea M Newtson, Deepti Sharma, Megan E McDonald, Matthew E Keeney, Brian J Smith, David P Bender, Michael J Goodheart, Kristina W Thiel, Eric J Devor, Kimberly K Leslie, Jesus Gonzalez Bosquet
The utility of comprehensive surgical staging in patients with low risk disease has been questioned. Thus, a reliable means of determining risk would be quite useful. The aim of our study was to create the best performing prediction model to classify endometrioid endometrial cancer (EEC) patients into low or high risk using a combination of molecular and clinical-pathological variables. We then validated these models with publicly available datasets. Analyses between low and high risk EEC were performed using clinical and pathological data, gene and miRNA expression data, gene copy number variation and somatic mutation data...
March 9, 2019: International Journal of Molecular Sciences
Enrico Vizza, Emanuela Mancini, Valentina Laquintana, Rossella Loria, Mariantonia Carosi, Ermelinda Baiocco, Lucia Cicchillitti, Giulia Piaggio, Lodovico Patrizi, Isabella Sperduti, Ashanti Zampa, Giuseppe Cutillo, Rita Falcioni, Giacomo Corrado
BACKGROUND: The aim of this study was to evaluate the prognostic role of positive peritoneal cytology (PPC) in a cohort of patients with endometrial cancer (EC). The secondary objective was to correlate the PPC and the expression of L1CAM in a group of patients with recurrence endometrial disease. METHODS: All women diagnosed with EC and who performed a peritoneal cytology at "Regina Elena" National Cancer Institute of Rome from 2001 to 2013 were included in the study...
March 2019: Surgical Oncology
Choa Park, Jeonggeun Lee, Byounguk Kong, Joonwoo Park, Heewon Song, KeunOh Choi, Taeeun Guon, YoungJoo Lee
Endocrine-disrupting chemicals (EDCs) are widely used in various consumer goods. Consequently, humans are constantly exposed to EDCs, which is associated with a variety of endocrine-related diseases. In this study, we demonstrated the effects of bisphenol A (BPA), benzyl butyl phthalate (BBP), and di(2-ethylhexyl) phthalate (DEHP) on estrogen receptor alpha (ERα) expression under normoxia and hypoxia. First, we confirmed the effects of EDCs on ER activity using OECD Test Guideline 455. Compared to the 100% activity induced by 1 nM 17-β-estradiol (positive control), BPA and BBP exhibited 50% ERα activation at concentrations of 1...
February 21, 2019: Environmental Pollution
Yusuke Matoba, Kouji Banno, Iori Kisu, Kosuke Tsuji, Daisuke Aoki
Endometrial cancer is diagnosed by endometrial biopsy, imaging and hysteroscopy, but it is sometimes difficult to find small lesions. 5-aminolevulinic acid (5ALA) forms protoporphyrin IX, which accumulates in tumors and fluoresces upon light irradiation. 5ALA is used for photodynamic diagnosis (PDD) for detecting malignant diseases. A 60-year-old female was suspected to have endometrial cancer by magnetic resonance imaging. Endometrial biopsy was performed repeatedly, but no malignant lesion was detected. We performed hysteroscopic 5ALA-PDD and could detect a small lesion...
March 5, 2019: Photodiagnosis and Photodynamic Therapy
Lisa Gahyun Bang, Venkata Ramesh Dasari, Dokyoon Kim, Radhika P Gogoi
Endometrial cancer (EMCA) is a clinically heterogeneous disease. Previously, we tested the efficacy of Verteporfin (VP) in EMCA cells and observed cytotoxic and anti-proliferative effects. In this study, we analyzed RNA sequencing data to investigate the comprehensive transcriptomic landscape of VP treated Type 1 EMCA cell lines, including HEC-1-A and HEC-1-B. There were 549 genes with differential expression of two-fold or greater and P < 0.05 after false discovery rate correction for the HEC-1-B cell line...
March 7, 2019: Scientific Reports
Adélaïde Racin, Emilie Raimond, Sofiane Bendifallah, Krystel Nyangoh Timoh, Lobna Ouldamer, Geoffroy Canlorbe, Nina Hudry, Charles Coutant, Olivier Graesslin, Cyril Touboul, Pierre Collinet, Alexandre Bricou, Cyrille Huchon, Martin Koskas, Marcos Ballester, Emile Daraï, Jean Levêque, Vincent Lavoue
BACKGROUND: Pelvic and paraaortic lymphadenectomy are recommended for women with high-intermediate, high-risk and advanced endometrial cancer (EC). Lymphadenectomy is less frequently performed in elderly patients than in younger patients. We examined the survival of elderly women diagnosed with high-risk EC according to whether lymphadenectomy was performed or not. METHODS: We selected women over 70 years with high-intermediate risk, high-risk or advanced EC from a multicenter retrospective cohort of women diagnosed between 2001 and 2013...
February 18, 2019: European Journal of Surgical Oncology
Fuminori Kimura, Akie Takebayashi, Mitsuaki Ishida, Akiko Nakamura, Jun Kitazawa, Aina Morimune, Kimiko Hirata, Akimasa Takahashi, Shoko Tsuji, Akiko Takashima, Tsukuru Amano, Shunichiro Tsuji, Tetsuo Ono, Shoji Kaku, Kyoko Kasahara, Suzuko Moritani, Ryoji Kushima, Takashi Murakami
AIM: Chronic endometritis (CE) is a disease of continuous and subtle inflammation characterized by the infiltration of plasma cells in the endometrial stromal area. Although the clinical significance of CE has been thought in clinical practice for a long time because it is either asymptomatic or presents with subtle symptoms, recent studies have shown the potential adverse effects of CE on fertility. In the present review, we focus on the concept, diagnosis, etiology, pathophysiology, diagnosis, impact on reproduction and treatment for it to understand CE...
March 6, 2019: Journal of Obstetrics and Gynaecology Research
Tahereh Arablou, Ali-Akbar Delbandi, Sepideh Khodaverdi, Soheila Arefi, Roya Kolahdouz-Mohammadi, Sahel Heydari, Tahereh Mohammadi, Naheed Aryaeian
Resveratrol, a phytoalexin polyphenol, has antiproliferative, antiangiogenic, anti-inflammatory, and antioxidant properties. The present study has assessed the effect of resveratrol treatment on the expression of insulin-like growth factor-1 (IGF-1) and hepatocyte growth factor (HGF) in endometrial stromal cells (ESCs) from women with and without endometriosis. Endometrial tissues were obtained from 40 endometriotic patients and 15 nonendometriotic control women. After the enzymatic digestion, 13 eutopic ESCs (EuESCs), 8 ectopic ESCs (EESCs), and 11 control ESCs (CESCs) were treated with resveratrol (100 μM) for 6, 24, and 48 hr...
March 6, 2019: Phytotherapy Research: PTR
Lu Han, Jiang Du, Lanbo Zhao, Chao Sun, Qi Wang, Xiaoqian Tuo, Huilian Hou, Yu Liu, Qing Wang, Qurat Ulain, Shulan Lv, Guanjun Zhang, Qing Song, Qiling Li
Recently, the research on early detection of precancerous change and endometrial carcinoma has been focusing on minimally invasive procedures for screening. On this basis, we aim to verify the feasibility of endometrial samplers for screening endometrial cancer using Li Brush. We recruited patients undergoing hysterectomy for different diseases from the Inpatient Department of the Department of Obstetrics and Gynecology. Before surgery, endometrial cells were collected by Li Brush. The cytopathologic diagnosis from Li Brush and the histopathologic diagnosis from hysterectomy in the same patient were compared to calculate sensitivity (Se), specificity (Sp), false-negative rate (FNR), false-positive rate (FPR), positive predictive value (PV+) %, and negative predictive value (PV-)...
2019: Frontiers in Oncology
Siriwan Tangjitgamol, Thannaporn Kittisiam, Jittrind Sriraumpuch
OBJECTIVE: To evaluate the prognostic role of metastatic lymph node (MLN), total lymph nodes (TLN) and MLN to TLN ratio in advanced-stage endometrial cancer (EMC). -Methods: EMC patients who had complete surgical staging between January 1995 and May 2017 and were in stage IIIC-IVB with pelvic and/or para-aortic nodal metastasis were identified. Data collected were age, stage, histopathology, TLN and MLN numbers, adjuvant treatment, disease status, and living status. The association of lymph node ratio (LNR), presented as a percentage of MLN to TLN, and other clinicopathologic factors with progression-free survival (PFS) and cancer-specific survival (CSS) was studied...
March 5, 2019: Gynecologic and Obstetric Investigation
Fadwa A Elsayed, Carli M J Tops, Maartje Nielsen, Dina Ruano, Hans F A Vasen, Hans Morreau, Frederik J Hes, Tom van Wezel
BACKGROUND: Germline mutations affecting the exonuclease domains of POLE and POLD1 predispose to colorectal adenomas and carcinoma. Here, we aimed to screen the exonuclease domains to find the genetic causes of multiple colorectal polyps in unexplained cases. METHODS: Using a custom next-generation sequencing panel, we sequenced the exonuclease domains of POLE and POLD1 in 332 index patients diagnosed with multiple colorectal polyps without germline alteration in colorectal polyposis predisposing genes...
March 2, 2019: Molecular Genetics & Genomic Medicine
Takaya Shiozaki, Maiko Miwa, Toshiko Sakuma, Kazuhiro Suzuki, Ai Kogiku, Kasumi Yamamoto, Tokihiro Senda, Hiroko Yano, Miho Kitai, Kazuko Matsuoka, Tamotsu Sudo, Shoji Nagao, Satoshi Yamaguchi
OBJECTIVE: We conducted a retrospective study to evaluate the correlation between pre-operative and post-operative histological diagnoses on endometrial cancer, and to describe the treatments and outcomes when post-operative diagnoses are downgraded from pre-operative histology. METHODS: Patients who underwent surgery for endometrial cancer in our facility between 2010 and 2013 were enrolled in the study. The definition of downgrade discordance is in accordance with the following criteria: 1) the pre-operative and post-operative histological diagnoses were both endometrioid and the final pathology was a lower grade than the pre-operative pathology and 2) the pre-operative diagnosis was not endometrioid, whereas the post-operative diagnosis was endometrioid grade 2 or less...
January 4, 2019: International Journal of Gynecological Cancer
Ka Ying Bonnie Ng, Ying Cheong
Tubal factors account for approximately 25% of cases of infertility, and the most severe manifestation of tubal disease is hydrosalpinx, accounting for 10-30% of tubal diseases. Hydrosalpinx is a distension or dilatation of the fallopian tube in the presence of a distal tubal occlusion, and the most common cause is pelvic inflammatory disease. Women with hydrosalpinges have lower implantation and pregnancy rates in assisted reproductive technology (ART), due to a combination of mechanical and chemical factors thought to disrupt the endometrial environment...
January 29, 2019: Best Practice & Research. Clinical Obstetrics & Gynaecology
Kangfeng Jiang, Shuai Guo, Jing Yang, Junfeng Liu, Aftab Shaukat, Gan Zhao, Haichong Wu, Ganzhen Deng
Endometritis is one of the main diseases that causes great economic losses in the dairy industry. Recent studies have shown that matrine extracted from the traditional Chinese herb Sophora flavescens is an alkaloid with a broad range of bioactivities. Here, we aimed to investigate the protective effects of matrine on Staphylococcus aureus lipoteichoic acid (LTA)-induced endometritis in mice and elucidate the possible molecular mechanisms in vitro. Histopathological changes showed that matrine remarkably attenuated the uterus injury in a mouse model of LTA-induced endometritis...
February 26, 2019: International Immunopharmacology
Prathiba M De Silva, Ioannis D Gallos
Endometrial cancer is the most common gynaecological malignancy amongst the developed world. The incidence of its precursor, endometrial hyperplasia, is estimated to be more than three times greater, and if left untreated, can progress to cancer (Reed et al. Am J Obstet Gynecol 2009;200:678). Successful treatment for endometrial hyperplasia has been found in the form of progestogens which modify the proliferative effects of unopposed oestrogen action on the endometrium, thus achieving disease regression. This article is protected by copyright...
March 1, 2019: BJOG: An International Journal of Obstetrics and Gynaecology
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