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zebrafish xenograft

Xiao-Yu Zhu, Dian-Wu Guo, Qiao-Cong Lao, Yi-Qiao Xu, Zhao-Ke Meng, Bo Xia, Hua Yang, Chun-Qi Li, Ping Li
Furanodiene is a natural terpenoid isolated from Rhizoma Curcumae, a well-known Chinese medicinal herb that presents anticancer effects in various types of cancer cell lines. In this study, we have successfully established zebrafish xenografts with 5 various human cancer cell lines; and validated these models with anti-cancer drugs used clinically for treating human cancer patients. We found that Furanodiene was therapeutically effective for human JF 305 pancreatic cancer cells and MCF-7 breast cancer cells xenotranplanted into zebrafish...
March 14, 2019: Scientific Reports
Daiki Yamamoto, Daisuke Sato, Hiroko Nakayama, Yuki Nakagawa, Yasuhito Shimada
Zebrafish embryos and larvae have become popular vertebrate models because their body walls are transparent, which enables live imaging of target organs using fluorescent protein transgenes or dye staining. Software packages for the quantification of these fluorescent signals are available from both commercial and noncommercial sources; however, their algorithms are complicated and their resources (code) have mostly not been openly shared. In this study, we developed a simple and robust open-source software tool named "ZF-Mapper" for the quantification of the fluorescence intensity of each pixel in zebrafish images with batch image file processing capability...
March 11, 2019: Zebrafish
Senyi Deng, ChuanFen Zhang, LiNa Yang, Liang Ma
Honokiol, a biphenolic neolignan with inappreciable toxicity isolated from Magnolia officinalis, has been reported to have antiangiogenic and antitumor properties in several tumor cell lines and tumor xenograft models. In our previous study, structural modification by chemical synthesis has been carried out to develop novel honokiol derivatives to improve antitumor activity and clarify the structure-activity relationship. Honokiol analogs, especially 3,5'-diformylated honokiol HK-(CHO)2, have been found to moderately block the newly grown segmental vessels from the dorsal aorta in the transgenic zebrafish-based assay, show antiangiogenic property, and exert medium cytotoxicity against two lung cell lines (Lewis lung carcinoma LL/2 cells and human non-small-cell lung cancer A549 cells)...
February 21, 2019: Anti-cancer Drugs
Chon-Kit Chou, Hurng-Wern Huang, Chun-Feng Yang, Hans-Uwe Dahms, Shih-Shin Liang, Tsu-Nai Wang, Po-Lin Kuo, Edward Hsi, Eing-Mei Tsai, Chien-Chih Chiu
Di(2-ethylhexyl)phthalate (DEHP) has been considered as an estrogen receptor alpha (ERα) agonist due to its ability to interact with ERα and promote the cell proliferation of ERα-positive breast cancer cells. The impact of DEHP on the chemical therapy in breast cancer is little known. Two breast cancer cell lines, MCF-7 (ERα-dependent) and MDA-MB-231 (ERα-independent) were examined. We found that DEHP impaired the effectiveness of camptothecin (CPT) and alleviated the CPT-induced formation of reactive oxygen species in ERα-positive MCF-7 cells, but not in ERα-negative MDA-MB-231 cells...
February 5, 2019: Environmental Toxicology
Zeynep Kabakci, Simon Käppeli, Claudio Cantù, Lasse D Jensen, Christiane König, Janine Toggweiler, Christian Gentili, Giovanni Ribaudo, Giuseppe Zagotto, Konrad Basler, Lorenzo A Pinna, Giorgio Cozza, Stefano Ferrari
CDC25 phosphatases play a key role in cell cycle transitions and are important targets for cancer therapy. Here, we set out to discover novel CDC25 inhibitors. Using a combination of computational methods, we defined a minimal common pharmacophore in established CDC25 inhibitors and performed virtual screening of a proprietary library. Based on the availability of crystal structures for CDC25A and CDC25B, we implemented a molecular docking strategy and carried out hit expansion/optimization. Enzymatic assays revealed that naphthoquinone scaffolds were the most promising CDC25 inhibitors among selected hits...
February 4, 2019: Scientific Reports
Jin Long Liu, Jing Li, Jia Jia Xu, Fei Xiao, Peng Lei Cui, Zhi Guang Qiao, Xiao Ling Zhang
Background Several miRNAs have been found expressed differentially in osteosarcoma (OS), thus they may function in the onset and progression of osteosarcoma. Method The osteosarcoma cell proliferation ability was evaluated by CCK8 assay, cell cycle and colony formation assay in vitro and xenograft NOD mice model in vivo. The osteosarcoma cell mortality was analyzed by wound healing assay and transwell invasion assay in vitro and by zebrafish cancer metastasis model in vivo. The level of miR-144 were detected by real-time PCR and ISH...
January 23, 2019: Molecular Pharmacology
Tung-Cheng Chang, Po-Li Wei, Precious Takondwa Makondi, Wei-Ting Chen, Chien-Yu Huang, Yu-Jia Chang
Advanced colorectal cancer (CRC) survival rates are still low despite advances in cytotoxic and targeted therapies. The development of new effective or alternative therapies is therefore urgently needed. Bromelain, an extract of pineapple, was shown to have anticancer effects, but its mechanisms in CRC have not been fully explored. Therefore, the roles of bromelain in CRC progression were investigated using different CRC cell lines, a zebrafish model, and a xenograft mouse model. The anticancer mechanisms were explored by assessing the role of bromelain in inducing reactive oxygen species (ROS), superoxide, autophagosomes, and lysosomes...
2019: PloS One
Nusrat Khan, Nilesh Kumar Mahajan, Pradip Sinha, Giridhara R Jayandharan
Zebrafish is emerging as a promising model for the study of human cancers. Several xenograft models of zebrafish have been developed, particularly in larval stages (<48 h post fertilization) when the immune system of fish is not developed. However, xenografting in adult zebrafish requires laborious and transient methods of immune suppression (γ- irradiation or dexamethasone) that limits engraftment and survival of the tumor or fail to recapitulate specific characteristics of malignancies. Thus, the availability of a simple protocol to successfully engraft adult zebrafish, remains a challenge...
March 2019: Blood Cells, Molecules & Diseases
Yumei Li, Qiong Wu, Gengnan Yu, Li Li, Xuanhao Zhao, Xiaoting Huang, Wenjie Mei
In this study, four polypyridyl ruthenium(II) complexes, namely, [(L1 )2 RuL2 ]·2ClO4 (1: L1  = phen, L2  = o-TFPIP, 2: L1  = bpy, L2  = o-TFPIP, 3: L1  = phen, L2  = o-MOPIP, and 4: L1  = bpy, L2  = o-MOPIP), were synthesized with different phenanthroimidazole derivatives, and their inhibitory activities were tested against various cancer cells. Among the Ru(II) complexes, 1 excellently inhibited the proliferation and induced the apoptosis of HepG2 cell. Importantly, 1 was mainly distributed in the cell mitochondria and markedly induced the dissipation of mitochondrial membrane potential, possibly attributing to DNA damage induced by the Ru(II) complexes...
December 22, 2018: European Journal of Medicinal Chemistry
Berney Peng, Mohammad Almeqdadi, Fabrice Laroche, Shajesh Palantavida, Maxim Dokukin, Jatin Roper, Omer H Yilmaz, Hui Feng, Igor Sokolov
Characterization data of fluorescent nanoparticles made of cellulose acetate (CA-dots) are shown. The data in this article accompanies the research article " Ultrabright fluorescent cellulose acetate nanoparticles for imaging tumors through systemic and topical applications " [1]. The measurements and calculation of brightness of individual CA-dots are presented. The description of conjugation procedure Pluronic F127-Folic Acid copolymer and folic acid is shown. Identification of composition of CA dots using Raman and absorbance spectroscopy is demonstrated...
February 2019: Data in Brief
Yipu Fan, Weikang Xue, Melitta Schachner, Weijiang Zhao
Malignant gliomas are the most aggressive forms of brain tumors; whose metastasis and recurrence contribute to high rates of morbidity and mortality. Glioma stem cell-like cells are a subpopulation of tumor-initiating cells responsible for glioma tumorigenesis, metastasis, recurrence and resistance to therapy. Epidermal growth factor receptor (EGFR) has been reported to be dysregulated in most cancers, including gliomas and its functions are closely linked to initiating tumor metastasis and a very poor prognosis...
December 26, 2018: Cancers
Xuanhao Zhao, Li Li, Gengnan Yu, Shuangyan Zhang, Yumei Li, Qiong Wu, Xiaoting Huang, Wenjie Mei
Polypyridine Ru(II) complexes have long been deemed to excellent antitumor agents that inhibit the proliferation of breast cancer cells. Nevertheless, their effects on the metastatic potency of breast cancer cells need further research. Herein, a class of polypyridine Ru(II) complexes coordinated with phenazine derivates (DPPZ) ([Ru(bpy)2 (DPPZ-R)](ClO4 )2 , Ru(bpy) 2 DPPZ : R = -H, Ru(bpy) 2 BrDPPZ : R = -Br, Ru(bpy) 2 MDPPZ : R = -CH3 , Ru(bpy)2BnDPPZ : R = -acene, Ru(bpy) 2 BEDPPZ : R = -C ≡ C(C6 H5 )) was synthesized by introducing different substituent groups to regulate the electron cloud density and planarity of the main ligands...
2019: Computational and Structural Biotechnology Journal
Claudio Fenizia, Cinzia Bottino, Silvia Corbetta, Raffaella Fittipaldi, Pamela Floris, Germano Gaudenzi, Silvia Carra, Franco Cotelli, Giovanni Vitale, Giuseppina Caretti
SMYD3 is a methylase previously linked to cancer cell invasion and migration. Here we show that SMYD3 favors TGFβ-induced epithelial-mesenchymal transition (EMT) in mammary epithelial cells, promoting mesenchymal and EMT transcription factors expression. SMYD3 directly interacts with SMAD3 but it is unnecessary for SMAD2/3 phosphorylation and nuclear translocation. Conversely, SMYD3 is indispensable for SMAD3 direct association to EMT genes regulatory regions. Accordingly, SMYD3 knockdown or its pharmacological blockade with the BCI121 inhibitor dramatically reduce TGFβ-induced SMAD3 association to the chromatin...
December 14, 2018: Nucleic Acids Research
David Hill, Lanpeng Chen, Ewe Snaar-Jagalska, Bill Chaudhry
Cancer metastasis is the most important prognostic factor determining patient survival, but currently there are very few drugs or therapies that specifically inhibit the invasion and metastasis of cancer cells. Currently, human cancer metastasis is largely studied using transgenic and immunocompromised mouse xenograft models, which are useful for analysing end-point tumour growth but are unable to accurately and reliably monitor in vivo invasion, intravasation, extravasation or secondary tumour formation of human cancer cells...
2018: F1000Research
Qiong Wu, Kangdi Zheng, Xiaoting Huang, Li Li, Wenjie Mei
Tanshinone-IIA (Tan-IIA), a primary active component extracted from commonly used Chinese herbal, Salvia miltiorrhiza (Danshen), is considered as a potential inhibitor against tumor cell proliferation. However, the potential application of Tan-IIA is hindered by its poor water solubility and low bioavailability. In this work, an imidazole moiety was linked to the skeleton of Tan-IIA to enhance its antitumor activity. A series of Tan-IIA-based analogues TA01-TA12 were synthesized, and their inhibitory activities against the migration and invasion of MDA-MB-231 cells were investigated...
November 19, 2018: Journal of Medicinal Chemistry
Denver D Britto, Barbara Wyroba, Wenxuan Chen, Rhoswen A Lockwood, Khanh B Tran, Peter R Shepherd, Christopher J Hall, Kathryn E Crosier, Philip S Crosier, Jonathan W Astin
Tumour angiogenesis has long been a focus of anti-cancer therapy; however, anti-angiogenic cancer treatment strategies have had limited clinical success. Tumour-associated myeloid cells are believed to play a role in the resistance of cancer towards anti-angiogenesis therapy, but the mechanisms by which they do this are unclear. An embryonic zebrafish xenograft model has been developed to investigate the mechanisms of tumour angiogenesis and as an assay to screen anti-angiogenic compounds. In this study, we used cell ablation techniques to remove either macrophages or neutrophils and assessed their contribution towards zebrafish xenograft angiogenesis by quantitating levels of graft vascularisation...
November 29, 2018: Disease Models & Mechanisms
John T Gamble, Yuriyah Reed-Harris, Carrie L Barton, Jane La Du, Robert Tanguay, Juliet A Greenwood
Glioblastoma (GBM) is a deadly disease due to its ability to quickly invade and destroy brain tissue. Slowing or stopping GBM cell progression is crucial to help those inflicted with the disease. Our lab created an embryo-larval zebrafish xenograft model as a tool to study human GBM progression in an observable brain environment. The zebrafish brain is a dynamic and complex environment providing an optimal setting for studying GBM cell progression. Here we demonstrate the ability of our model to quantitate GBM proliferation, dispersal, blood vessel association, microtumor formation, and individual cell invasion by evaluating the importance of an extracellular matrix protein, laminin alpha 5 (lama5), on U251MG cell progression...
December 2, 2018: Biochemical and Biophysical Research Communications
S Verykiou, M Alexander, N Edwards, R Plummer, B Chaudhry, P E Lovat, D S Hill
BACKGROUND: Patients with malignant melanoma often relapse following treatment with BRAF and/or MEK inhibitors (MEKi) due to development of drug resistance by melanoma subpopulations, mediated through induction of generic survival mechanisms. OBJECTIVES: The aim of this study was to establish the temporal pattern of CD271 regulation (a stem cell marker that mediates tumour aggressiveness) during development of resistance by melanoma to the MEKi trametinib, and to determine the association between development of resistance to trametinib and induction of pro-survival autophagy...
October 19, 2018: British Journal of Dermatology
Chung-I Yu, Chung-Yi Chen, Wangta Liu, Po-Chih Chang, Chiung-Wei Huang, Kuang-Fen Han, In-Pin Lin, Mei-Ying Lin, Chien-Hsing Lee
Presently, natural sources and herbs are being sought for the treatment of human oral squamous cell carcinoma (OSCC) in order to alleviate the side effects of chemotherapy. This study investigates the effect of sandensolide, a cembrane isolated from Sinularia flexibilis , to inhibit human OSCC cell growth with the aim of developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (Ca9.22, SCC9 and HSC-3 cell lines) and oral normal cells (HGF-1), as well as a zebrafish xenograft model, were used to test the cytotoxicity of sandensolide (MTT assay), as well as to perform cell cycle analysis and Western blotting...
October 16, 2018: Marine Drugs
Fabiola Porta, Daniel Ehrsam, Claudia Lengerke, Henriette E Meyer Zu Schwabedissen
Cytotoxic compounds used to treat cancer are often associated with adverse events. The development of formulations activated by tumor-specific triggers would allow a reduction of systemic exposure while maintaining therapeutic concentrations in the tumor. One enzyme with proteolytic activity reported to be involved in tumor progression and assumed to be enhanced in the tumor environment is the matrixmetalloproteinase 9 (MMP-9). In our study, we aimed to develop surface modified PDMS-PMOXA polymersomes able to release their cytotoxic payload upon digestion by MMP-9...
October 1, 2018: Molecular Pharmaceutics
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