keyword
https://read.qxmd.com/read/38522587/pharmacokinetic-evaluation-of-a-single-chain-antibody-fragment-against-scorpion-toxins-in-sheep
#1
JOURNAL ARTICLE
Roberto Olivares Hernández, Lidia Riaño Umbarila, Baltazar Becerril, Alejandro Alagón Cano, Hilda Vazquez Lopez
A key aspect during the development of antivenoms is the evaluation of the efficiency and security of the therapeutic molecules. In this work, we report the pharmacokinetic analysis of a neutralizing single chain antibody fragment named LR (scFv LR) where three sheep were used as a large animal model. The animals were injected through i.v. route with 2 mg of scFv LR. Blood samples were drawn every minute within the first 15 min, the sampling continues at 20, 25, 30, 45, 60, 90, 120 min, subsequently at one-hour intervals, 3, 4, 5, 6 h, two more samples at 9 and 12 h and, two more samples at 24 and 48 hours and finally at one-day intervals during 4 days...
March 22, 2024: Toxicon: Official Journal of the International Society on Toxinology
https://read.qxmd.com/read/38511431/identification-and-bioactivity-evaluation-of-ring-opening-and-lactone-forms-of-enterolactone-from-sheep-fed-flaxseed-cake
#2
JOURNAL ARTICLE
Cong-Cong Zhuang, Chun-Rui Liu, Hai-Yan Xu, Xin-Yue Yang, Dan-Dan Wu, Zhong Zheng, Li-Wei Wang, Zheng Liu, Chao-Mei Ma
The previously undescribed lactone ring-opening enterolactone and its sulphate were purified along with the lactone counterparts from the urine of dairy sheep fed flaxseed cake. The structures were determined by NMR and MS analyses. The ring-opening and lactone forms underwent mutual transformation with changes in pH and milk could protect the lactone form. Enterolactone exhibited more effective anti-proliferation activity on MDA-MB-231 breast cancer cells than its ring-opening counterpart, while the ring-opening enterolactone demonstrated more effective anti-osteoporosis activity than the lactone form...
March 21, 2024: Natural Product Research
https://read.qxmd.com/read/38404205/streptavidin-biotin-system-mediated-immobilization-of-a-bivalent-nanobody-onto-magnetosomes-for-separation-and-analysis-of-3-phenoxybenzoic-acid-in-urine
#3
JOURNAL ARTICLE
Fang Tang, Yating Wang, Di Wang, Yayun Yang, Jiashu Chang, Huabo Sun, Shaopeng Gu, Jinxin He
The compound 3-phenoxybenzoic acid (3-PBA) is frequently utilized as a biomarker to detect exposure to various pyrethroids. In this study, a bivalent nanobody (Nb2) specifically targeting 3-PBA was biotinylated and immobilized onto streptavidin (SA)-modified bacterial magnetic nanoparticles (BMPs), resulting in the formation of BMP-SA-Biotin-Nb2 complexes. These complexes demonstrated remarkable stability when exposed to strongly acidic solutions (4 M HCl), methanol (80%), and high ionic strength (1.37 M NaCl)...
February 26, 2024: Analytical Methods: Advancing Methods and Applications
https://read.qxmd.com/read/38384949/multi-omics-analysis-on-the-mechanism-of-the-effect-of-isatis-leaf-on-the-growth-performance-of-fattening-sheep
#4
JOURNAL ARTICLE
Zhikun Cao, Mingliang Yi, Jialu Zhou, Zhiyu Zhang, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Yinghui Ling, Zijun Zhang, Hongguo Cao
INTRODUCTION: This study evaluated the effects of Isatis Leaf (ISL) on the growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum and urine metabolites, and rumen epithelial tissue transcriptome of fattening sheep. METHODS: Twelve 3.5-month-old healthy fattening sheep were randomly divided into two groups, each with 6 replicates, and fed with basal diet (CON) and basal diet supplemented with 80 g/kg ISL for 2.5 months...
2024: Frontiers in Veterinary Science
https://read.qxmd.com/read/38366060/nitrogen-and-energy-utilization-and-methane-emissions-of-sheep-grazing-on-annual-pasture-vs-native-pasture
#5
JOURNAL ARTICLE
Kaili Xie, Fuyao Liu, Cheng Zhang, Fujiang Hou
The purpose of this study was to evaluate the differences in annual pasture and native pasture on dry matter (DM) intake, nutrient digestibility, nitrogen (N) and energy utilization, and methane (CH4) emission of grazing sheep, and to provide basis for rational livestock grazing in salinized regions. The study used 10 male Hu sheep ♀ × thin-tailed Han sheep ♂ rams (20±5 kg) aged 5 months. Sheep grazing was conducted in annual pasture and native pasture using a 2×2 Latin square design...
February 15, 2024: Journal of Animal Science
https://read.qxmd.com/read/38362676/hepatic-and-renal-lesions-in-sheep-intoxicated-with-urochloa-hybrid-mulato-ii-in-argentina
#6
JOURNAL ARTICLE
Raúl E Marin, Eduardo J Gimeno, Franklin Riet-Correa, Francisco A Uzal
A flock of 48 sheep in Argentina grazing on a pasture of hybrid Urochloa (formerly Brachiaria ) Mulato II ( Urochloa ruziziensis  ×  Urochloa decumbens  ×  Urochloa brizantha ) developed facial dermatitis, severe jaundice, and weakness after brief physical activity. Blood biochemistry of 3 animals revealed azotemia, elevated aspartate aminotransferase activity, and increased direct, indirect, and total bilirubin concentrations. The urine was markedly turbid and contained large concentrations of bile pigments and protein...
February 16, 2024: Journal of Veterinary Diagnostic Investigation
https://read.qxmd.com/read/38343188/animal-species-and-blood-identification-with-peptide-mass-fingerprinting
#7
JOURNAL ARTICLE
Akihisa Igoh, Masanobu Miura, Satoru Miyaishi
As more families are acquiring pets and the opportunities for wild animals to appear in human neighborhoods are increasing, the number of cases and accidents involving animals is increasing. Hence, the need to identify animal species from blood left over at accident sites is increasing. Human hemoglobin is used as a marker for human blood. Although tandem mass spectrometry is the dominant methodology used in proteomics research, peptide mass fingerprinting, given its instant applicability, may be useful for screening animal species, as the amino acid sequences of hemoglobin from various animals differ...
February 11, 2024: Analytical Chemistry
https://read.qxmd.com/read/38244281/modified-tube-cystostomy-technique-for-management-of-obstructive-urolithiasis-in-small-ruminants-procedure-and-outcome-in-17-sheep-and-goats
#8
JOURNAL ARTICLE
Rachel E Oman, Luis Rivero, Leslie F Weaver, Katharine M Simpson
OBJECTIVE: To describe the surgical technique and clinical outcome of small ruminants treated for obstructive urolithiasis using a modified tube cystostomy (MTC) technique. ANIMALS: 15 goats and 2 sheep treated with an MTC between March 2018 and February 2023. CLINICAL PRESENTATION: Animals were diagnosed with obstructive urolithiasis on the basis of history, physical examination, and ultrasonographic examination. An MTC was performed with sedation and a local block...
February 1, 2024: Journal of the American Veterinary Medical Association
https://read.qxmd.com/read/38237645/fangji-huangqi-decoction-ameliorates-membranous-nephropathy-through-the-upregulation-of-bnip3-mediated-mitophagy
#9
JOURNAL ARTICLE
Yuxin Wang, Yuhua Ma, Yanrong Ke, Xiaocheng Jiang, Jian Liu, Yang Xiao, Hong Zheng, Chaojun Wang, Xue Chen, Manman Shi
ETHNOPHARMACOLOGICAL RELEVANCE: Fangji Huangqi Decoction (FJHQ), a traditional Chinese medicinal formula outlined in Zhang Zhongjing's "Jin Gui Yao Lue" during the Han Dynasty, is often used to treat conditions characterized by symptoms like edema and dysuria, including membranous nephropathy (MN). Despite its proven clinical effectiveness, the exact mechanisms through which FJHQ acts on MN remain elusive. AIM OF THE STUDY: This study aimed to investigate whether FJHQ enhances BNIP3-mediated mitophagy in podocytes by promoting BNIP3 expression and whether this improvement leads to the amelioration of MN...
January 16, 2024: Journal of Ethnopharmacology
https://read.qxmd.com/read/38099683/draft-genome-sequences-of-listeria-monocytogenes-strains-of-sequence-type-1733-which-constituted-a-pseudo-outbreak-due-to-contaminated-blood-agar-media
#10
JOURNAL ARTICLE
Sangmi Lee, Asmaa Sadat, Zuzana Kucerova, Sophia Kathariou
We report genome sequences of Listeria monocytogenes sequence type (ST) 1733 from a 2013 pseudo-outbreak, where L. monocytogenes isolation from non-sterile sites (urine, wound, or abscess) was an artifact from contaminated sheep blood in the isolation media. Two ST1733 strains from wound and urine in 2005 are also reported.
December 15, 2023: Microbiology Resource Announcements
https://read.qxmd.com/read/38027399/is-camel-s-urine-friend-or-enemy-review-of-its-role-in-human-health-or-diseases
#11
REVIEW
Mohamed Tharwat, Tariq I Almundarij, Madeh Sadan, Faten Khorshid, Ayman Swelum
Camels play an important role in the pastoral mode of life by fulfilling basic demands of livelihood. Various pathologies, such as tuberculosis, hemorrhoids, ascites, increased size of the abdomen, gas colic, anemia, and abdominal tumors, were treated with animal urine, including camels, horses, donkeys, sheep, goats, elephants, and buffalo. Thirty different compounds were analyzed in camel urine by gas chromatography and mass spectrometry. For inductively coupled plasma mass spectrometry analysis, 28 important elements were analyzed in the urine of both camel and bovine...
October 2023: Open Veterinary Journal
https://read.qxmd.com/read/38003078/multi-omics-analysis-of-the-mechanism-of-mentha-haplocalyx-briq-on-the-growth-and-metabolic-regulation-of-fattening-sheep
#12
JOURNAL ARTICLE
Mingliang Yi, Zhikun Cao, Jialu Zhou, Yinghui Ling, Zijun Zhang, Hongguo Cao
Mentha haplocalyx Briq (MHB) and its components have been proven to improve the growth performance of livestock and poultry. The aim of this experiment was to investigate the effects of MHB addition on growth performance, rumen and fecal microbiota, rumen fluid, serum and urine metabolism, and transcriptomics of rumen epithelial cells in meat sheep. Twelve Hu sheep were selected for the experiment and fed with basic diet (CON) and a basal diet supplemented with 80 g/kg DM of Mentha haplocalyx Briq (MHB). The experimental period was 10 weeks with the first 2 weeks as the pre-trial period...
November 9, 2023: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/37921801/combined-inhibition-of-phosphodiesterases-5-and-9-in-experimental-heart%C3%A2-failure
#13
JOURNAL ARTICLE
Miriam T Rademaker, Nicola J A Scott, Christopher J Charles, A Mark Richards
BACKGROUND: Intracellular second messenger cyclic guanosine monophosphate (cGMP) mediates bioactivity of the natriuretic peptides and nitric oxide, and is key to circulatory homeostasis and protection against cardiovascular disease. Inhibition of cGMP-degrading phosphodiesterases (PDEs) PDE5 and PDE9 are emerging as pharmacological targets in heart failure (HF). OBJECTIVES: The present study investigated dual enhancement of cGMP in experimental HF by combining inhibition of PDE5 (P5-I) and PDE9 (P9-I)...
October 16, 2023: JACC. Heart Failure
https://read.qxmd.com/read/37744903/symbiotic-diazotrophs-in-response-to-yak-grazing-and-tibetan-sheep-grazing-in-qinghai-tibetan-plateau-grassland-soils
#14
JOURNAL ARTICLE
Shengnan Sun, Yi Zhao, Quanmin Dong, Xiaoxia Yang, Yuzhen Liu, Wentao Liu, Guang Shi, Wenting Liu, Chunping Zhang, Yang Yu
Grazing by local livestock is the traditional human practice in Qinghai-Tibetan Plateau grassland, and moderate intensity grazing can maintain high productivity and diversity of alpine grassland. Grazing ecosystems are often nitrogen-limited, but N2 -fixing communities in response to yak grazing and Tibetan sheep grazing in Qinghai-Tibetan Plateau grassland have remained underexplored. In this study, we applied quantitative PCR quantitation and MiSeq sequencing of nifH under yak grazing and Tibetan grazing through a manipulated grazing experiment on an alpine grassland...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/37692998/artificial-placenta-support-of-extremely-preterm-ovine-fetuses-at-the-border-of-viability-for-up-to-336-hours-with-maintenance-of-systemic-circulation-but-reduced-somatic-and-organ-growth
#15
JOURNAL ARTICLE
Haruo Usuda, Hideyuki Ikeda, Shimpei Watanabe, Shinichi Sato, Erin L Fee, Sean W D Carter, Yusaku Kumagai, Yuya Saito, Tsukasa Takahashi, Yuki Takahashi, Shinichi Kawamura, Takushi Hanita, Masatoshi Saito, Atsuo Kikuchi, Mahesh A Choolani, Nobuo Yaegashi, Matthew W Kemp
Introduction: Artificial placenta therapy (APT) is an experimental life support system to improve outcomes for extremely preterm infants (EPI) less than 1,000 g by obviating the need for pulmonary gas exchange. There are presently no long-term survival data for EPI supported with APT. To address this, we aimed to maintain 95d-GA (GA; term-150d) sheep fetuses for up to 2 weeks using our APT system. Methods: Pregnant ewes ( n = 6) carrying singleton fetuses underwent surgical delivery at 95d GA. Fetuses were adapted to APT and maintained for up to 2 weeks with constant monitoring of key physiological parameters and extensive time-course blood and urine sampling, and ultrasound assessments...
2023: Frontiers in Physiology
https://read.qxmd.com/read/37589061/molecular-typing-of-leptospira-spp-in-farmed-and-wild-mammals-reveals-new-host-serovar-associations-in-new-zealand
#16
JOURNAL ARTICLE
D A Wilkinson, M Edwards, C Shum, M Moinet, N E Anderson, J Benschop, S Nisa
AIMS: To apply molecular typing to DNA isolated from historical samples to determine Leptospira spp. infecting farmed and wild mammals in New Zealand. MATERIALS AND METHODS: DNA samples used in this study were extracted from urine, serum or kidney samples (or Leptospira spp. cultures isolated from them) collected between 2007 and 2017 from a range of domestic and wildlife mammalian species as part of different research projects at Massey University. Samples were included in the study if they met one of three criteria: samples that tested positive with a lipL32 PCR for pathogenic Leptospira ; samples that tested negative by lipL32 PCR but were recorded as positive to PCR for pathogenic Leptospira in the previous studies; or samples that were PCR-negative in all studies but were from animals with positive agglutination titres against serogroup Tarassovi...
August 17, 2023: New Zealand Veterinary Journal
https://read.qxmd.com/read/37566566/molecular-detection-of-coxiella-burnetii-in-raw-meat-samples-collected-from-different-abattoirs-in-districts-kasur-and-lahore-of-punjab-pakistan
#17
JOURNAL ARTICLE
Shahpal Shujat, Wasim Shehzad, Aftab Ahmad Anjum, Julia A Hertl, Muhammad Yasir Zahoor, Yrjö T Gröhn
Coxiella burnetii is the zoonotic pathogen that causes Q fever; it is widespread globally. Livestock animals are its main reservoir, and infected animals shed C. burnetii in their birth products, feces, vaginal mucus, urine, tissues, and food obtained from them, i.e., milk and meat. There were previously very few reports on the prevalence of C. burnetii in raw meat. This study aimed to determine the prevalence of C.burnetii and its molecular characterization in raw ruminant meat from the Kasur and Lahore districts in Punjab, Pakistan, as this has not been reported so far...
2023: PloS One
https://read.qxmd.com/read/37565514/brief-early-life-angiotensin-converting-enzyme-inhibition-attenuates-the-diuretic-response-to-saline-loading-in-sheep-with-solitary-functioning-kidney
#18
JOURNAL ARTICLE
Zoe McArdle, Reetu R Singh, Karen Moritz, Michiel F Schreuder, Kate M Denton
A solitary functioning kidney (SFK) from birth predisposes to hypertension and kidney dysfunction, and this may be associated with impaired fluid and sodium homeostasis. Brief and early angiotensin converting enzyme inhibition (ACEi) in a sheep model of SFK delays onset of kidney dysfunction. We hypothesized that modulation of the renin angiotensin system via brief postnatal ACEi in SFK would reprogram renal sodium and water handling. Here, blood pressure (BP), kidney haemodynamics and kidney excretory function were examined in response to an isotonic saline load (0...
August 11, 2023: Clinical Science (1979-)
https://read.qxmd.com/read/37251257/the-uptake-of-selenium-by-perennial-ryegrass-in-soils-of-different-organic-matter-contents-receiving-sheep-excreta
#19
JOURNAL ARTICLE
Pei-Tzu Kao, Heather L Buss, Steve P McGrath, Tegan Darch, Helen E Warren, Michael R F Lee
BACKGROUND AND AIMS: The intake of selenium, an essential element for animals and humans, in ruminants is largely determined by selenium concentration in ingested forages, which take up selenium mainly from soil. Ruminant excreta is a common source of organic fertilizer, which provides both nutrients and organic matter. This study aims to unentangle the unclear effect of applying different types of ruminant excreta in soils of different organic matter contents on selenium uptake by forage...
2023: Plant and Soil
https://read.qxmd.com/read/37207113/alterations-in-nutrient-digestion-and-utilization-associated-with-different-residual-feed-intake-in-hu-sheep
#20
JOURNAL ARTICLE
Hongbo Zeng, Yuyang Yin, Lingxi Chen, Zhuoxin Xu, Yang Luo, Qian Wang, Bin Yang, Jiakun Wang
Improving feed efficiency is crucial to the animal industry. Residual feed intake (RFI) is now regarded as an index of feed efficiency evaluation and is independent of growth characteristics. Our study aims to explore the alterations in growth performance and nutrient digestion in Hu sheep with different RFI phenotypes. Sixty-four male Hu sheep (body weight = 24.39 ± 1.12 kg; postnatal days = 90 ± 7.9) were selected for the study. After an evaluation period of 56 days and power analysis, samples were collected from 14 low RFI (L-RFI group, power = 0...
June 2023: Animal Nutrition
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