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https://read.qxmd.com/read/31331464/a-zebrafish-tale-of-parabiosis-podocytes-and-proteinuria
#1
COMMENT
Brooke E Chambers, Rebecca A Wingert
Glomerular damage is a harbinger of kidney dysfunction. Circulating permeability factors are implicated in causing primary and secondary damage to podocytes, leading to proteinuria and eventual progression to the nephrotic syndrome, but the mechanisms are not well understood. Müller-Deile et al. employed parabiosis with zebrafish embryos and found that a damaged glomerulus can impact a healthy one in a shared circulatory system. This methodology shows promise for elucidating kidney injury pathways in response to systemic disease...
August 2019: Kidney International
https://read.qxmd.com/read/31076096/circulating-factors-cause-proteinuria-in-parabiotic-zebrafish
#2
JOURNAL ARTICLE
J Müller-Deile, H Schenk, P Schroder, K Schulze, P Bolaños-Palmieri, F Siegerist, N Endlich, H Haller, M Schiffer
Proteinuria can be induced by impairment of any component of the glomerular filtration barrier. To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholino-mediated knockdown of a podocyte specific gene (nephronectin) was induced in one zebrafish larva which was then fused to an un-manipulated fish. Notably, proteinuria and glomerular damage were present in the manipulated fish and in the parabiotically-fused partner...
March 8, 2019: Kidney International
https://read.qxmd.com/read/29184141/development-and-growth-of-organs-in-living-whole-embryo-and-larval-grafts-in-zebrafish
#3
JOURNAL ARTICLE
Toshihiro Kawasaki, Akiteru Maeno, Toshihiko Shiroishi, Noriyoshi Sakai
Age-related systemic environments influence neurogenesis and organ regeneration of heterochronic parabiotic partners; however, the difficulty of manipulating small embryos prevents the effects of aged systemic environments on primitive organs at the developmental stage from being analysed. Here, we describe a novel transplantation system to support whole living embryos/larvae as grafts in immunodeficient zebrafish by the intrusion of host blood vessels into the grafts, allowing bodies similar to those of heterochronic parabiosis to be generated by subcutaneous grafting...
November 28, 2017: Scientific Reports
https://read.qxmd.com/read/28796253/tnf-signaling-and-macrophages-govern-fin-regeneration-in-zebrafish-larvae
#4
JOURNAL ARTICLE
Mai Nguyen-Chi, Béryl Laplace-Builhé, Jana Travnickova, Patricia Luz-Crawford, Gautier Tejedor, Georges Lutfalla, Karima Kissa, Christian Jorgensen, Farida Djouad
Macrophages are essential for appendage regeneration after amputation in regenerative species. The molecular mechanisms through which macrophages orchestrate blastema formation and regeneration are still unclear. Here, we use the genetically tractable and transparent zebrafish larvae to study the functions of polarized macrophage subsets during caudal fin regeneration. After caudal fin amputation, we show an early and transient accumulation of pro-inflammatory macrophages concomitant with the accumulation of non-inflammatory macrophages which, in contrast to pro-inflammatory macrophages, remain associated to the fin until the end of the regeneration...
August 10, 2017: Cell Death & Disease
https://read.qxmd.com/read/27341538/generation-of-parabiotic-zebrafish-embryos-by-surgical-fusion-of-developing-blastulae
#5
JOURNAL ARTICLE
Elliott J Hagedorn, Jennifer L Cillis, Caitlyn R Curley, Taylor C Patch, Brian Li, Bradley W Blaser, Raquel Riquelme, Leonard I Zon, Dhvanit I Shah
Surgical parabiosis of two animals of different genetic backgrounds creates a unique scenario to study cell-intrinsic versus cell-extrinsic roles for candidate genes of interest, migratory behaviors of cells, and secreted signals in distinct genetic settings. Because parabiotic animals share a common circulation, any blood or blood-borne factor from one animal will be exchanged with its partner and vice versa. Thus, cells and molecular factors derived from one genetic background can be studied in the context of a second genetic background...
June 11, 2016: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/23377378/generating-parabiotic-zebrafish-embryos-for-cell-migration-and-homing-studies
#6
JOURNAL ARTICLE
Doris Lou Demy, Zachary Ranta, Jean-Michel Giorgi, Magali Gonzalez, Philippe Herbomel, Karima Kissa
Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.
March 2013: Nature Methods
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