Read by QxMD icon Read

granulation tissue

Mohammad Mohajeri, Petri T Kovanen, Vanessa Bianconi, Matteo Pirro, Arrigo F G Cicero, Amirhossein Sahebkar
Mast cells are tissue-resident cells, which have been proposed to participate in various inflammatory diseases, among them the cardiovascular diseases (CVDs). For mast cells to be able to contribute to an inflammatory process, they need to be activated to exocytose their cytoplasmic secretory granules. The granules contain a vast array of highly bioactive effector molecules, the neutral protease tryptase being the most abundant protein among them. The released tryptase may act locally in the inflamed cardiac or vascular tissue, so contributing directly to the pathogenesis of CVDs...
March 12, 2019: Pharmacology & Therapeutics
Catherine McLaughlin, John Clements, Ana-Maria Oprişoreanu, Sergiy Sylantyev
KEY POINTS: A T258F mutation of the glycine receptor increases the receptor affinity to endogenous agonists, modifies single-channel conductance and shapes response decay kinetics. Glycine receptors of cerebellar granule cells play their functional role not continuously, but when granule cell layer starts receiving high amount of excitatory inputs. Despite their relative scarcity, tonically active glycine receptors of cerebellar granule cells make a significant impact on action potential generation, inter-neuronal crosstalk, and modulate synaptic plasticity in neural networks; extracellular glycine increases probability of postsynaptic response occurrence acting at NMDA receptors and decreases this probability acting at glycine receptors...
March 15, 2019: Journal of Physiology
Reza Vaghardoost, Yaser Ghavami, Behnam Sobouti
BACKGROUND: Burn is one of the most common injuries and the 4th common cause of trauma globally. This study was designed to evaluate the effect of the Mentha pulegium extract on second degree burn injuries in rats assessing histopathologic and macroscopic. METHODS: M. Pulegium extract dressings was used as a treatment to deep dermal contact burns in rats, compared with two control groups of vaseline dressing and simple dressing by normal saline. After creating second-degree burn on the dorsum of rats, the treatments were applied for 15 min in three groups...
January 2019: World Journal of Plastic Surgery
Arnon Pudgerd, Charoonroj Chotwiwatthanakun, Thanapong Kruangkum, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana, Rapeepun Vanichviriyakit
The hematopoietic organ (HO) of the giant freshwater prawn Macrobrachium rosenbergii is a discrete, whitish mass located in the epigastric region of the cephalothorax, posterior to the brain. It is composed of hematopoietic cells arranged in a thick layer of numerous lobules that surround a central hemal sinus from which they are separated by a thin sheath. At the center of the sinus is the muscular cor frontale. The lobules extend radially outward from the sinus in three developmental zones. Basal Zone 1 nearest the sinus contains large hematopoietic stem cells with euchromatic nuclei that stain positive for proliferation cell nuclear antigen (PCNA)...
March 11, 2019: Fish & Shellfish Immunology
Cornelia Wiegand, Martin Abel, Uta-Christina Hipler, Peter Elsner
Non-adhering dressings are commonly used during granulation, tissue formation, and re-epithelialization. Elucidating cytotoxic effects and influence on proliferation/migration capacity of cells like fibroblasts is of interest. Dressings' effects were investigated by comprehensive in vitro approach: (1) MTT assay measuring cell viability after direct contact, (2) ATP assay determining effects on cell proliferation, and (3) scratch wound assay featuring an in vitro wound healing model. One cotton-based dressing with vaseline (vas) was included in the study and four polyester dressings containing vas and technology-lipido-colloid matrix (TLC), carboxymethylcellulose (CMC), hydrocolloid (HC), or glycerin (gly) as additives...
March 13, 2019: Scientific Reports
Sharada Sawant, Harsh Dongre, Deepak Kanojia, Sayli Jamghare, Anita Borges, Milind Vaidya
Early detection of altered epithelium can help in controlling the further progression by timely intervention. Alterations in cellular adhesion are one of the hallmarks of cancer progression, which can be detected at the intracellular level using high-resolution electron microscopy. This study aimed to evaluate the role of electron microscopy in the establishment of ultrastructural markers for early detection of altered epithelium using tissues from 4-Nitroquinoline-1-Oxide (4NQO) induced rat tongue carcinogenesis...
March 14, 2019: Microscopy and Microanalysis
Óscar Mariño-Crespo, Elisa Cuevas-Álvarez, Amy L Harding, Craig Murdoch, Almudena Fernández-Briera, Emilio Gil-Martín
Aims and experimental design. The acute-phase protein haptoglobin (Hp) has been recently detected in colorectal cancer (CRC) tissue, where its expression correlates with metastasis. Recently, we identified Hp as a CDw75 antigen-expressing protein in colorectal tissue. To deepen the knowledge of this protein in CRC, we studied the expression of Hp in healthy and tumour tissue specimens from 62 CRC patients by immunohistochemistry and Western blotting, as well as in the Caco-2 and HT-29 CRC cell lines by quantitative PCR, immunofluorescence microscopy and flow cytometry...
March 13, 2019: Histology and Histopathology
Itamar Goren, Yvette Köhler, Ahmed Aglan, Josef Pfeilschifter, Karl-Friedrich Beck, Stefan Frank
The gaseous mediators nitric oxide (NO), carbon monoxide (CO) and lately also hydrogen sulfide (H2 S) have been described to contribute to the interplay of protein type- and lipid mediators in the regulation of wound healing. In particular, the recently reported role of H2 S in skin repair remains largely unresolved. Therefore we assessed the expressional kinetics of potential H2 S-producing enzymes during undisturbed skin repair: the cystathionine-γ-lyase (CSE), the cystathionine-β-synthase (CBS) and the 3-mercaptopyruvate sulfurtransferase (MPST)...
March 9, 2019: Nitric Oxide: Biology and Chemistry
Jérôme Cléach, Philippe Pasdois, Philippe Marchetti, Denis Watier, Guillaume Duflos, Emmanuelle Goffier, Anne-Sophie Lacoste, Christian Slomianny, Thierry Grard, Philippe Lencel
The current methods used to routinely assess freshness in the fishing industry reflect more a state of spoilage than a state of freshness. Mitochondria, the seat of cellular respiration, undergo profound changes in post mortem tissues. The objective of this study was to demonstrate that mitochondrial activity constitutes a putative early fish freshness marker. The structure of gilthead sea bream (Sparus aurata) muscle tissue was evaluated over time by transmission electron microscopy. Respiration was assessed in mitochondria isolated from sea bream fillets using oxygraphy...
July 30, 2019: Food Chemistry
Yu S Esakov, A A Pechetov, T N Khlan, E N Solodinina, K V Slepenkova, S A Bessonova, K V Lukich, P I Davydenko
AIM: To present single-center experience and results of surgical treatment of acquired tracheal stenosis. MATERIAL AND METHODS: There were 99 patients with acquired tracheal stenosis for the period from January 2008 to December 2017. Median age was 39 (28; 55) years (range 19-79 years), male/female ratio - 64/35. There were 59 patients with tracheostomy-related stenosis, 31 - post-intubation injury, 6 - posttraumatic stenosis, malignant and idiopathic stenosis was observed in 2 and 1 patients, respectively...
2019: Khirurgiia
Chen Meng, Zhongxiao Zhang, Xia Liu, Xiuli Yan, Chong Shi, Na Liu, Xinxin Wang, Jing Ma
RATIONALE: Broncholithiasis is a rare disease and is characterized by calcification in the tracheobronchial tree. This disease has a predilection in the adult and occurrence is rare in children. Considering its infrequency, we report a rare clinical case, with the aim of sharing our experience during the diagnosis and treatment procedures. PATIENT CONCERNS: We report a 9-year- and 6-month-old girl who complained of chronic cough and recurrent wheeze for 2 months...
March 2019: Medicine (Baltimore)
Hodaka Sasaki, Akishige Hokugo, Lixin Wang, Kenzo Morinaga, John T Ngo, Hiroko Okawa, Ichiro Nishimura
The circadian clock, which consists of endogenous self-sustained and cell-autonomous oscillations in mammalian cells, is known to regulate a wide range of peripheral tissues. The unique upregulation of a clock gene, neuronal PAS domain protein2 (Npas2), observed along with fibroblast aging prompted us to investigate the role of Npas2 in the homeostasis of dermal structure using in vivo and in vitro wound healing models. Time-course healing of a full-thickness punched wound was monitored in C57Bl6J (WT) and Npas2 KO mice...
March 9, 2019: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Shigeki Suzuki, Takao Fukuda, Shintaro Nagayasu, Jun Nakanishi, Kazuma Yoshida, Shizu Hirata-Tsuchiya, Yuki Nakao, Tomomi Sano, Akiko Yamashita, Satoru Yamada, Kouji Ohta, Hideki Shiba, Fusanori Nishimura
It is well known that dental pulp tissue can evoke some of the most severe acute inflammation observed in the human body. We found that dental pulp cells secrete a factor that induces tumor necrosis factor-α production from macrophages, and designated this factor, dental pulp cell-derived powerful inducer of TNF-α (DPIT). DPIT was induced in dental pulp cells and transported to recipient cells via microvesicles. Treatment of dental pulp cells with a PKR inhibitor markedly suppressed DPIT activity, and weak interferon signals were constitutively activated inside the cells...
March 7, 2019: Scientific Reports
Alexander Charlton, Noor Janjua, Darius Rejali
Necrotising otitis externa (NOE) is an infection originating in the soft tissues of the external auditory canal (EAC) spreading to the surrounding bone and rarely causing intracranial complications. It is usually caused by Pseudomonas aeruginosa and has historically occurred in elderly patients with diabetes or immunodeficiency. EAC foreign body is a risk factor for otitis externa but has not been described in NOE. A healthy 31-year-old man presented with new-onset seizures and worsening left-sided otalgia and otorrhoea...
March 6, 2019: BMJ Case Reports
George Gonçalves Dos Santos, Luisa Queiroz Vasconcelos, Suelen Cristina da Silva Poy, Renata Dos Santos Almeida, Aryon de Almeida Barbosa Júnior, Silvia Rachel de Albuquerque Santos, Alexandre Malta Rossi, Fúlvio Borges Miguel, Fabiana Paim Rosa
PURPOSE: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. METHODS: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8...
February 28, 2019: Acta Cirúrgica Brasileira
Xin-Ming Gao, Yang Zhou, Dan-Dan Zhang, Cong-Cong Hou, Jun-Quan Zhu
The large yellow croaker (Larimichthys crocea) is a marine fish that is economically important to Chinese fisheries, and its reproductive and developmental biology have been extensively investigated. However, the molecular mechanism of oogenesis in L. crocea is not clear. Here, we investigated the multiple vitellogenin (Vtg) system in large yellow croaker. Three different vtg cDNA sequences, including vtgAa, vtgAb and vtgC, were cloned, which indicate the existence of multiple Vtg proteins in large yellow croaker (Lc-Vtgs)...
March 7, 2019: Fish Physiology and Biochemistry
Shichang Mu, Qiaoqiao Hua, Yangyang Jia, Ming-Wei Chen, Yizhong Tang, Datong Deng, Yong He, Chunlin Zuo, Fang Dai, Honglin Hu
OBJECTIVE: To investigate the effect of negative-pressure wound therapy (NPWT) on the circulating number of endothelial progenitor cells (EPCs) in diabetic patients with mild to moderate degrees of ischemic foot ulcer. METHODS: We selected 84 diabetic patients who had a foot ulcer with a duration of at least four weeks and who had an ankle-brachial index of 0.5-0.9. Patients were assigned to one two groups according to 2:1 randomization: NPWT group ( n = 56) and non-NPWT (patients who did not receive NPWT) group ( n = 28)...
March 6, 2019: Vascular
Michèle-Léa Sigal, Azeddine Addala, Hervé Maillard, Maxime Chahim, Florent Sala, Sophie Blaise, Sophie Dalac, Sylvie Meaume, Serge Bohbot, Clémence Tumba, Olivier Tacca
OBJECTIVE: To assess the efficacy, safety and acceptability of a new TLC-NOSF dressing with poly-absorbent fibres in the management of exuding leg ulcers, at the different stages of healing. METHOD: This work presents the results of two prospective, multicentric clinical studies: NEREIDES and CASSIOPEE. Patients with a non-infected, moderate-to-strongly exudating leg ulcer of venous or mixed origin, were treated with the dressing and an appropriate compression system for 12 weeks...
March 3, 2019: Journal of Wound Care
Lene Rydal Sveen, Gerrit Timmerhaus, Aleksei Krasnov, Harald Takle, Sigurd Handeland, Elisabeth Ytteborg
Skin biopsies (5 mm) taken from behind the dorsal fin on Atlantic salmon post-smolts were followed over a 2 month period. The healing process was dominated by hemostasis, acute inflammation, and epidermal repair the first 14 days post wounding (dpw), as shown through imaging, histological evaluation, and transcriptomics. Most of the immune genes showed decreased expression after two weeks, approaching the levels of intact skin, as also reflected in sections where reduced inflammation in the wound bed was observed...
March 5, 2019: Scientific Reports
Jing Zhao, Yuen-Cheung Chan, Bao He, Ting-Ting Duan, Zhi-Ling Yu
ETHNOPHARMACOLOGY RELEVANCE: Yi-Shen-Hua-Shi (YSHS) granule is a modern Chinese patent drug for treating chronic glomerulonephritis (CGN). It is derived from a traditional Chinese medicine formula Sheng-Yang-Yi-Wei decoction that is used to treat CGN in ancient China. Pharmacological activities of YSHS granule have not been reported. In this work, we investigated the anti-CGN effects and TGFβ signaling-related mechanism of action of this herbal drug. MATERIALS AND METHODS: The rat model of CGN was established by injection of cationization-bovine serum albumin (C-BSA) for five weeks...
March 2, 2019: Journal of Ethnopharmacology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"