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Zhen He, Jian Jun Yang, Rong Zhang, Hua Ting Li, Liang Wu, Feng Jiang, Wei Ping Jia, Cheng Hu
AIM: The early screening of non-alcoholic fatty liver disease (NAFLD) is of great significance for early detection and intervention in NAFLD. MicroRNAs (miRNAs) are important regulators of metabolic disease, including NAFLD. The aim of this study was to examine the association of serum miR-29a-c with NAFLD in a Chinese population. METHODS: 276 individuals were enrolled in this study and divided into non-diabetic and diabetic groups. Real-time PCR was performed to quantify the serum miR-29a-c expression levels...
February 12, 2019: Journal of Digestive Diseases
Lihua Liu, Linlin Liu, Sijing Lu
Considering the joint contribution of long non‑coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs) to tumorigenesis, the aim of the present study was to investigate whether and how lncRNA H19 targets miR‑29b‑3p to affect the progression of lung adenocarcinoma by the modulation of signal transducer and activator of transcription 3 (STAT3). A total of 305 lung adenocarcinoma tissues and four human lung adenocarcinoma cell lines (i.e. Calu‑3, NCI‑H1975, A549 and NCI‑H23) were used. pcDNA3.1‑H19, short interfering RNA (si‑)H19, miR‑29b‑3p mimic, miR‑29b‑3p inhibitor and negative control (NC) were transfected into the cells, and the proliferation, viability and apoptosis of the cells were determined using a Cell Counting Kit‑8 assay, colony formation assay and flow cytometry, respectively...
January 24, 2019: International Journal of Oncology
Olga D'Almeida, Omar Mothar, Esther Apraku Bondzie, Yolande Lieumo, Laure Tagne, Sumeet Gupta, Thomas Volkert, Stuart Levine, Jean-Bosco Tagne
BACKGROUND: MicroRNAs are noncoding RNA molecules of ~ 22 nucleotides with diagnostic and therapeutic action [Curr Drug Targets, 2015. 16(12): p. 1381-403], affecting the expression of mRNAs involved in invasion, migration, and development [Oncotarget, 2015. 6(9): p. 6472-98, Cancer Manag Res, 2014. 6: p. 205-16]. miR-200c is part of the miR-200c/141 cluster on chromosome 12p13. Its mechanism of action when encapsulated is critical in lung cancer when patients express changes in miRNAs...
February 11, 2019: BMC Cancer
Michela Vitiello, Giuseppe Palma, Mario Monaco, Anna Maria Bello, Simona Camorani, Paola Francesca, Domenica Rea, Antonio Barbieri, Gennaro Chiappetta, Gabriella De Vita, Laura Cerchia, Claudio Arra, Monica Fedele
PATZ1 is a transcriptional factor downregulated in thyroid cancer whose re-expression in thyroid cancer cells leads to a partial reversion of the malignant phenotype, including the capacity to proliferate, migrate, and undergo epithelial-to-mesenchymal transition. We have recently shown that PATZ1 is specifically downregulated downstream of the Ras oncogenic signaling through miR-29b, and that restoration of PATZ1 in Ha-Ras transformed FRTL5 rat thyroid cells is able to inhibit their capacities to proliferate and migrate in vitro...
February 9, 2019: Genes
Huilin Wang, Zheng Li, Jingyan Gao, Qingwu Liao
Oxygen-glucose deprivation (OGD)-activated microglia contribute to neuronal apoptosis via releasing pro-inflammatory cytokines, and some miRNAs have been reported to be involved in this process. Circular RNAs (circRNAs) have been reported to function as miRNA sponges, but it remains unknown whether and how circRNAs contribute to OGD-activated microglia-induced neuronal apoptosis. Here, we investigated the function and relationship of miR-29b and circPTK2 in OGD-activated microglia-induced neuronal apoptosis...
February 8, 2019: International Journal of Biological Macromolecules
Tian Su, Yuzhong Xiao, Ye Xiao, Qi Guo, Changjun Li, Yan Huang, Qiufang Deng, Jingxiang Wen, Fangliang Zhou, Xiang-Hang Luo
Insulin resistance is the major pathological characteristic of type 2 diabetes and the elderly often develop insulin resistance. However, the deep-seated mechanisms for aging-related insulin resistance remain unclear. Here, we showed that nano-sized exosomes released by bone marrow mesenchymal stem cells (BM-MSCs) of aged mice could be taken up by adipocytes, myocytes, and hepatocytes, resulting in insulin resistance both in vivo and in vitro. Using microRNA (miRNA) array assays, we found that the amount of miR-29b-3p was dramatically increased in exosomes released by BM-MSCs of aged mice...
February 4, 2019: ACS Nano
Junichi Sakata, Takashi Sasayama, Kazuhiro Tanaka, Hiroaki Nagashima, Mitsutoshi Nakada, Hirotomo Tanaka, Naoya Hashimoto, Naoki Kagawa, Manabu Kinoshita, Satoshi Nakamizo, Masahiro Maeyama, Masamitsu Nishihara, Kohkichi Hosoda, Eiji Kohmura
BACKGROUND: MicroRNAs (miRs) regulate many biological processes, such as invasion, angiogenesis, and metastasis. Glioblastoma (GBM) patients with metastasis/metastatic dissemination have a very poor prognosis; therefore, inhibiting metastasis/metastatic dissemination has become an important therapeutic strategy for GBM treatment. METHODS: Using 76 GBM tissues, we examined the expression levels of 23 GBM-related miRs and compared the miRs' expression levels between GBMs with metastasis/metastatic dissemination and GBMs without metastasis/metastatic dissemination...
January 30, 2019: Journal of Neuro-oncology
Norio Ushio, Md Mahfuzur Rahman, Tadashi Maemura, Yu-Chang Lai, Tomoko Iwanaga, Hiroaki Kawaguchi, Noriaki Miyoshi, Yasuyuki Momoi, Naoki Miura
Inhibiting aberrantly upregulated microRNAs (miR/miRNAs) has emerged as a novel focus for therapeutic intervention in human melanoma. Thus, identifying upregulated miRNAs is essential for identifying additional melanoma-associated therapeutic targets. In the present study, microarray-based miRNA profiling of canine malignant melanoma (CMM) tissue obtained from the oral cavity was performed and differential expression was confirmed by a reverse transcription-quantitative polymerase chain reaction (RT-qPCR). An analysis of the microarray data revealed 17 dysregulated miRNAs; 5 were upregulated and 12 were downregulated...
January 2019: Oncology Letters
Sven H Loosen, Georg Lurje, Georg Wiltberger, Mihael Vucur, Alexander Koch, Jakob N Kather, Pia Paffenholz, Frank Tacke, Florian T Ulmer, Christian Trautwein, Tom Luedde, Ulf P Neumann, Christoph Roderburg
OBJECTIVES: Cholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy. Despite tremendous research activities, the prognosis for the majority of patients is still poor. Only in case of early diagnosis, liver resection might potentially lead to long-term survival. However, it is still unclear which patients benefit most from extensive liver surgery, highlighting the need for new diagnostic and prognostic stratification strategies. METHODS: Serum concentrations of a 4 miRNA panel (miR-122, miR-192, miR-29b and miR-155) were analyzed using semi-quantitative reverse-transcriptase PCR in serum samples from 94 patients with cholangiocarcinoma undergoing tumour resection and 40 healthy controls...
2019: PloS One
Kun Tang, Jianping Zhao, Jungang Xie, Jianmiao Wang
Chronic obstructive pulmonary disease (COPD) is a common chronic airway inflammatory disease. MicroRNAs are shown to be involved in the regulation of inflammation. We investigated the role of microRNA-29b (miR-29b) in the airway inflammation in COPD. The expression of miR-29b in the lung and plasma was examined. The target of miR-29b, BRD4, was predicted by online algorithms and verified in HBE cells. The expression of BRD4, interleukin (IL)-8 and IL-6 in the lung was also examined. The role of miR-29b in the inflammatory cytokine expression of airway epithelial cells was studied using an in vitro model system...
January 17, 2019: American Journal of Physiology. Lung Cellular and Molecular Physiology
Chunrong Zhao, Ying Miao, Zhen Cao, Jian Shi, Jianmei Li, Fei Kang, Ce Dou, Zhao Xie, Qiang Xiang, Shiwu Dong
OBJECTIVE: Chondrocyte hypertrophy, a terminal stage of chondrocyte differentiation, is essential to the endochondral bone formation and is one of the major pathological factors in osteoarthritis. This study investigated the role of microRNA-29b (miR-29b), which is involved in chondrogenesis, in the regulation of hypertrophy in chondrocytes. METHODS: miR-29b expression was assessed during murine mesenchymal stem cells (mMSCs) chondrogenesis. To detect whether miR-29b affects chondrocyte hypertrophy, the mMSCs induced toward chondrogenesis were transfected with miR-29b or its antisense inhibitor (antagomiR-29b)...
January 16, 2019: Journal of Cellular Biochemistry
Yanjie Wang, Junhua Zhang, Jian Li, Rong Gui, Xinmin Nie, Rong Huang
Hematopoietic stem cell transplantation is commonly used in patients with certain hematological or bone marrow tumors. Total body irradiation combined with chemotherapy is part of the preconditioning protocol that was the most commonly used before hematopoietic stem cell transplantation. However, total body irradiation preconditioning damages other normal cells in bone marrow. Therefore, exploring the mechanism of radiation resistance in bone marrow mesenchymal stem cells is of great significance for recovering the hematopoietic function after cell transplantation...
January 14, 2019: Bosnian Journal of Basic Medical Sciences
Yingzi Zhang, Bin Chen, Dazhuang Li, Xiaozhong Zhou, Zhiwei Chen
Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is quite important for the bone formation. Bone morphogenetic proteins (BMPs) are important to the skeleton formation. As a member of BMPs, BMP1 can induce bone and cartilage development. This study revealed the biological effect of BMP1 on the osteogenic differentiation. Firstly, the relative lower expression of BMP1 was detected in hBMSCs obtained from osteoporosis patients. The expression levels of osteoporosis-related genes (ALP, OCN, and OPN) were found to be decreased in hBMSCs treated with sh-BMP1...
December 31, 2018: Pathology, Research and Practice
Yasuo Takashima, Atsushi Kawaguchi, Yasuo Iwadate, Hiroaki Hondoh, Junya Fukai, Koji Kajiwara, Azusa Hayano, Ryuya Yamanaka
MicroRNAs (miRNAs) are small RNA molecules that inhibit gene function by suppressing translation of target genes. However, in primary central nervous system lymphoma (PCNSL), the biological significance of miRNAs is largely unknown, although some miRNAs are known to be prognosis markers. Here, we analyzed 847 miRNAs expressed in 27 PCNSL specimens using microarray profiling and surveyed miRNA signature for prognostic prediction. Of these, 16 miRNAs were expressed in 27 PCNSL specimens at a frequency of 48%...
2019: PloS One
Lihua Wang, Zhihua Wang, Liuye Huang, Chengrong Wu, Bo Zhang
Pancreatic cancer is one of the leading causes of solid carcinoma with the worst survival rate. The reasons for the worst survival rate include the lack of biomarkers for early detection, diagnosis at a late stage, and the limitation of the current therapy. Further study to investigate the underlying molecular mechanism in pancreatic cancer patients is necessary. A previous study showed that the miR-29b expression level is dysregulated, suggesting that it may serve an important function in pancreatic cancer...
December 31, 2018: Anti-cancer Drugs
Ines Maldonado-Lasuncion, Mercedes Atienza, Mayely P Sanchez-Espinosa, Jose L Cantero
Evidence has shown that microRNAs (miRNAs) are involved in molecular pathways responsible for aging and prevalent aging-related chronic diseases. However, the lack of research linking circulating levels of miRNAs to changes in the aging brain hampers clinical translation. Here, we have investigated if serum expression of brain-enriched miRNAs that have been proposed as potential biomarkers in Alzheimer's disease (AD) (miR-9, miR-29b, miR-34a, miR-125b, and miR-146a) are also associated with cognitive functioning and changes of the cerebral cortex in normal elderly subjects...
December 22, 2018: Cerebral Cortex
Miaoyang Hu, Xufeng Wei, Meng Li, Ling Tao, Liping Wei, Minxia Zhang, Hexiang Cheng, Yuan Yuan
OBJECTIVE: To investigate the expression profile of circular RNAs (circRNAs) and proposed circRNA-microRNA (miRNA) regulatory network in atrial fibrillation (AF). METHODS: Atrial tissues from patients with persistent AF with rheumatic heart disease and non-AF myocardium with normal hearts were collected for circRNA differential expression analyses by high-throughput sequencing. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the potential functions of the differentially expressed genes and AF-related pathways...
January 2019: Anatolian Journal of Cardiology
Shu Zhou, Dazhou Lei, Faqin Bu, Hongqiang Han, Shucai Zhao, Yan Wang
MicroRNAs participate in the regulation of abnormal cardiomyocyte apoptosis and autophagy, which leads to heart failure (HF). Lower miR-29b-3p levels were found in HF patients in this study. However, the role of miR-29b-3p in the molecular pathogenesis of HF remains unclear. Hypoxia-stimulated H9c2 cells were used an in vitro model of HF. It was found that hypoxia stimulation decreased the miR-29b-3p expression and enhanced cell apoptosis and autophagy response in H9c2 cells. While the effects of hypoxia on cell apoptosis and autophagy were reversed by miR-29b-3p transfection, especially 100 nM...
December 17, 2018: Journal of Cardiovascular Translational Research
Shaojuan Hao, Le Wang, Kun Zhao, Xiaodan Zhu, Fanglei Ye
BACKGROUND: MIAT may be implicated in the pathogenesis of age-related hearing loss (AHL). This study aimed to clarify the effect of a MIAT signaling pathway on the risk of AHL. METHODS: Terminal deoxynucleotidyl transferase dUTP nick-end labeling assay, auditory brainstem response (ABR) and quantitative hair cell counts were used to compare the hearing functions in different groups of mice. 5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) dye method was used to establish the potential association between mitochondrial dysfunction and aging...
December 16, 2018: Journal of Cellular Biochemistry
Ling-Xiang Yu, Bo-Lun Zhang, Yuan Yang, Meng-Chao Wang, Guang-Lin Lei, Yuan Gao, Hu Liu, Chao-Hui Xiao, Jia-Jia Xu, Hao Qin, Xiao-Ya Xu, Zi-Shuo Chen, Da-Dong Zhang, Fu-Gen Li, Shao-Geng Zhang, Rong Liu
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, and current treatments exhibit limited efficacy against advanced HCC. The majority of cancer-related deaths are caused by metastasis from the primary tumor, which indicates the importance of identifying clinical biomarkers for predicting metastasis and indicating prognosis. Patient-derived cells (PDCs) may be effective models for biomarker identification. In the present study, a wound healing assay was used to obtain 10 fast-migrated and 10 slow-migrated PDC cultures from 36 HCC samples...
October 29, 2018: Oncology Reports
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