Juthamas Jaroensuk, Chalermroj Sutthaphirom, Jittima Phonbuppha, Wachirawit Chinantuya, Chatchai Kesornpun, Nattanon Akeratchatapan, Narongyot Kittipanukul, Kamonwan Phatinuwat, Sopapan Atichartpongkul, Mayuree Fuangthong, Thunyarat Pongtharangkul, Frank Hollmann, Pimchai Chaiyen
Cofactor imbalance obstructs the productivities of metabolically engineered cells. Herein, we employed a minimally perturbing system, xylose reductase and lactose (XR/lactose), to increase levels of a pool of sugar-phosphates which are connected to the biosynthesis of NAD(P)H, FAD, FMN and ATP in Escherichia coli. The XR/lactose system could increase the amounts of the precursors of these cofactors and was tested with three different metabolically engineered cell systems (fatty alcohol biosynthesis, bioluminescence light generation and alkane biosynthesis) with different cofactor demands...
December 28, 2023: Journal of Biological Chemistry