keyword
https://read.qxmd.com/read/38481806/nk-92mi-cells-engineered-with-anti-claudin-6-chimeric-antigen-receptors-in-immunotherapy-for-ovarian-cancer
#21
JOURNAL ARTICLE
Junping Li, Hong Hu, Hui Lian, Shuo Yang, Manting Liu, Jinping He, Bihui Cao, Dongni Chen, Yuling Hu, Chen Zhi, Yan Shen, Xiaodie Ye, Bingjia He, Ming Zhao, Weijun Fan, Linfeng Xu, Rom Leidner, Qingde Wu, Lili Yang, Zhenfeng Zhang
Background : The application of chimeric antigen receptor (CAR) NK cells in solid tumors is hindered by lack of tumor-specific targets and inefficient CAR-NK cell efficacy. Claudin-6 (CLDN6) has been reported to be overexpressed in ovarian cancer and may be an attractive target for CAR-NK cells immunotherapy. However, the feasibility of using anti-CLDN6 CAR-NK cells to treat ovarian cancer remains to be explored. Methods : CLDN6 expression in primary human ovarian cancer, normal tissues and cell lines were detected by immunohistochemistry and western blot...
2024: International Journal of Biological Sciences
https://read.qxmd.com/read/38481695/activation-of-multiple-g-protein-pathways-to-characterize-the-five-dopamine-receptor-subtypes-using-bioluminescence-technology
#22
JOURNAL ARTICLE
Denise Mönnich, Laura J Humphrys, Carina Höring, Bradley L Hoare, Lisa Forster, Steffen Pockes
G protein-coupled receptors show preference for G protein subtypes but can recruit multiple G proteins with various downstream signaling cascades. This functional selection can guide drug design. Dopamine receptors are both stimulatory (D1 -like) and inhibitory (D2 -like) with diffuse expression across the central nervous system. Functional selectivity of G protein subunits may help with dopamine receptor targeting and their downstream effects. Three bioluminescence-based assays were used to characterize G protein coupling and function with the five dopamine receptors...
March 8, 2024: ACS Pharmacology & Translational Science
https://read.qxmd.com/read/38479166/discovery-of-5-aminopyrido-2-3-d-pyrimidin-7-8h-one-derivatives-as-new-hematopoietic-progenitor-kinase-1-hpk1-inhibitors
#23
JOURNAL ARTICLE
Xiaorong Qiu, Rong Liu, Huan Ling, Yang Zhou, Xiaomei Ren, Fengtao Zhou, Jinwei Zhang, Weixue Huang, Zhen Wang, Ke Ding
Hematopoietic progenitor kinase 1 (HPK1) is a negative regulator of T-cell receptor signaling. While HPK1 is considered as a promising target for cancer immunotherapy, no small-molecule HPK1 inhibitors have been approved for cancer treatment. Herein, we report the discovery of a series of new HPK1 inhibitors with a 5-aminopyrido[2,3-d]pyrimidin-7(8H)-one scaffold. The most potent compound 9f inhibited HPK1 kinase activity with an IC50 of 0.32 nM in the time-resolved fluorescence resonance energy transfer (TR-FRET) assays, while displayed reasonable selectivity in a panel of 416 kinases...
March 5, 2024: European Journal of Medicinal Chemistry
https://read.qxmd.com/read/38478496/environmental-preferences-and-critical-habitat-for-the-velvet-belly-lanternshark-etmopterus-spinax-in-icelandic-waters
#24
JOURNAL ARTICLE
Helga Mattína, Steven E Campana, Klara Jakobsdóttir
The velvet belly lanternshark (Etmopterus spinax) is a small, bioluminescent shark that is caught as bycatch in many deep-sea fisheries in the Atlantic Ocean. Using data from 10,597 seasonal research survey tows spanning 11 years, the distribution, relative abundance, life history, and environmental preferences of E. spinax in Icelandic waters was examined for the first time. E. spinax (n = 8774) were only captured in relatively deep offshore waters to the south and west of Iceland. Females grew to larger sizes than males and reached 50% sexual maturity at a total length of 50 cm...
2024: PloS One
https://read.qxmd.com/read/38474266/the-expression-and-functionality-of-cb-1-r-nmdar-complexes-are-decreased-in-a-parkinson-s-disease-model
#25
JOURNAL ARTICLE
Irene Reyes-Resina, Jaume Lillo, Iu Raïch, Joan Biel Rebassa, Gemma Navarro
One of the hallmarks of Parkinson's disease (PD) is the alteration in the expression and function of NMDA receptor (NMDAR) and cannabinoid receptor 1 (CB1 R). The presence of CB1 R-NMDAR complexes has been described in neuronal primary cultures. The activation of CB1 R in CB1 R-NMDAR complexes was suggested to counteract the detrimental NMDAR overactivation in an AD mice model. Thus, we aimed to explore the role of this receptor complex in PD. By using Bioluminescence Resonance Energy Transfer (BRET) assay, it was demonstrated that α-synuclein induces a reorganization of the CB1 R-NMDAR complex in transfected HEK-293T cells...
March 5, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473946/bioluminescence-of-r-cypridina-luciferin-with-cypridina-luciferase
#26
JOURNAL ARTICLE
Shusei Kanie, Chun Wu, Kiyohito Kihira, Rie Yasuno, Yasuo Mitani, Yoshihiro Ohmiya
Cypridina luciferin (CypL) is a marine natural product that functions as the luminous substrate for the enzyme Cypridina luciferase (CypLase). CypL has two enantiomers, ( R )- and ( S )-CypL, due to its one chiral center at the sec -butyl moiety. Previous studies reported that ( S )-CypL or racemic CypL with CypLase produced light, but the luminescence of ( R )-CypL with CypLase has not been investigated. Here, we examined the luminescence of ( R )-CypL, which had undergone chiral separation from the enantiomeric mixture, with a recombinant CypLase...
February 26, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38472726/cyclosporin-a-loaded-dissolving-microneedles-for-dermatitis-therapy-development-characterisation-and-efficacy-in-a-delayed-type-hypersensitivity-in-vivo-model
#27
JOURNAL ARTICLE
Miquel Martínez-Navarrete, Antonio José Guillot, Maria C Lobita, María Carmen Recio, Rosa Giner, Juan Aparicio-Blanco, María Carmen Montesinos, Hélder A Santos, Ana Melero
Several drugs can be used for treating inflammatory skin pathologies like dermatitis and psoriasis. However, for the management of chronic and long-term cases, topical administration is preferred over oral delivery since it prevents certain issues due to systemic side effects from occurring. Cyclosporin A (CsA) has been used for this purpose; however, its high molecular weight (1202 Da) restricts the diffusion through the skin structure. Here, we developed a nano-in-micro device combining lipid vesicles (LVs) and dissolving microneedle array patches (DMAPs) for targeted skin delivery...
March 12, 2024: Drug Delivery and Translational Research
https://read.qxmd.com/read/38469601/bioluminescence-the-vibrant-glow-of-nature-and-its-chemical-mechanisms
#28
JOURNAL ARTICLE
Stefan Schramm, Dieter Weiß
Bioluminescence, the mesmerizing natural phenomenon where living organisms produce light through chemical reactions, has long captivated scientists and laypersons alike, offering a rich tapestry of insights into biological function, ecology, evolution as well as the underlying chemistry. This comprehensive review systematically explores the phenomenon of bioluminescence, addressing its historical context, geographic dispersion, and ecological significance with a focus on their chemical mechanisms. We discuss terrestrial bioluminescence in various habitats, including fireflies in Central Europe, luminescent fungi in Brazil's Atlantic rainforest, and glowing species in New Zealand's Waitomo Caves and the Siberian Steppes...
March 12, 2024: Chembiochem: a European Journal of Chemical Biology
https://read.qxmd.com/read/38468592/adgrg1-an-adhesion-g-protein-coupled-receptor-forms-oligomers
#29
JOURNAL ARTICLE
Orkun Cevheroğlu, Berkay Demirbaş, Dilara Öğütcü, Merve Murat
G protein-coupled receptor (GPCR) oligomerization is a highly debated topic in the field. While initially believed to function as monomers, current literature increasingly suggests that these cell surface receptors, spanning almost all GPCR families, function as homo- or hetero-oligomers. Yet, the functional consequences of these oligomeric complexes remain largely unknown. Adhesion GPCRs (aGPCRs) present an intriguing family of receptors characterized by their large and multi-domain N-terminal fragments (NTFs), intricate activation mechanisms, and the prevalence of numerous splice variants in almost all family members...
March 12, 2024: FEBS Journal
https://read.qxmd.com/read/38466680/a-brittle-star-is-born-ontogeny-of-luminous-capabilities-in-amphiura-filiformis
#30
JOURNAL ARTICLE
Constance Coubris, Laurent Duchatelet, Sam Dupont, Jérôme Mallefet
Bioluminescence is the production of visible light by living organisms thanks to a chemical reaction, implying the oxidation of a substrate called luciferin catalyzed by an enzyme, the luciferase. The luminous brittle star Amphiura filiformis depends on coelenterazine (i.e., the most widespread luciferin in marine ecosystems) and a luciferase homologous to the cnidarian Renilla luciferase to produce blue flashes in the arm's spine. Only a few studies have focused on the ontogenic apparitions of bioluminescence in marine organisms...
2024: PloS One
https://read.qxmd.com/read/38465639/sensitive-bioluminescence-imaging-of-cryptococcosis-in-galleria-mellonella-improves-antifungal-screening-under-in-vivo-conditions
#31
JOURNAL ARTICLE
Eliane Vanhoffelen, Lori Vermoesen, Lauren Michiels, Katrien Lagrou, Agustin Reséndiz-Sharpe, Greetje Vande Velde
Cryptococcus neoformans is an environmental yeast that primarily affects immunocompromised individuals, causing respiratory infections and life-threatening meningoencephalitis. Treatment is complicated by limited antifungal options, with concerns such as adverse effects, dose-limiting toxicity, blood-brain barrier permeability, and resistance development, emphasizing the critical need to optimize and expand current treatment options against invasive cryptococcosis. Galleria mellonella larvae have been introduced as an ethical intermediate for in vivo testing, bridging the gap between in vitro antifungal screening and mouse studies...
December 2024: Virulence
https://read.qxmd.com/read/38465195/assessment-of-ecotoxicological-effects-of-fojo-coal-mine-waste-elutriate-in-aquatic-species-douro-coalfield-north-portugal
#32
JOURNAL ARTICLE
Aracelis Narayan, Bárbara S Diogo, Catarina Mansilha, Jorge Espinha Marques, Deolinda Flores, Sara C Antunes
Introduction: The exploitation of anthracite A in the Pejão mining complex (Douro Coalfield, North Portugal) resulted in the formation of several coal waste piles without proper environmental control. In 2017, a new pedological zonation emerged in the Fojo area, after the ignition and self-burning of some of the coal waste piles, namely: unburned coal waste (UW); burned coal waste, and a cover layer (BW and CL, respectively); uphill soil (US); mixed burned coal waste (MBW); downhill soil (DS). This study aimed to evaluate the toxic effects of 25 soil elutriates from different pedological materials...
2024: Frontiers in toxicology
https://read.qxmd.com/read/38464469/novel-estrogen-receptor-dimerization-bret-based-biosensors-for-screening-estrogenic-endocrine-disrupting-chemicals
#33
JOURNAL ARTICLE
Gyuho Choi, Hyunkoo Kang, Jung-Soo Suh, Haksoo Lee, Kiseok Han, Gaeun Yoo, Hyejin Jo, Yeong Min Shin, Tae-Jin Kim, BuHyun Youn
The increasing prevalence of endocrine-disrupting chemicals (EDCs) in our environment is a growing concern, with numerous studies highlighting their adverse effects on the human endocrine system. Among the EDCs, estrogenic endocrine-disrupting chemicals (eEDCs) are exogenous compounds that perturb estrogenic hormone function by interfering with estrogen receptor (ER) homo (α/α, β/β) or hetero (α/β) dimerization. To date, a comprehensive screening approach for eEDCs affecting all ER dimer forms in live cells is lacking...
2024: Biomaterials Research
https://read.qxmd.com/read/38462450/topology-of-ubiquitin-chains-in-the-chromatosomal-environment-of-the-e3-ubiquitin-ligase-rnf168
#34
JOURNAL ARTICLE
Anna A Kudriaeva, Lyudmila A Yakubova, George A Saratov, Vasiliy I Vladimirov, Valeriy M Lipkin, Alexey A Belogurov
Genome stability is critical for normal functioning of cells, it depends on accuracy of DNA replication, chromosome segregation, and DNA repair. Cellular defense mechanisms against DNA damage are important for preventing cancer development and aging. The E3 ubiquitin ligase RNF168 of the RING superfamily is an essential component of the complex responsible for ubiquitination of the H2A/H2A.X histones near DNA double-strand breaks, which is a key step in attracting repair factors to the damage site. In this study, we unequivocally showed that RNF168 does not have the ability to directly distinguish architecture of polyubiquitin chains, except for the tropism of its two ubiquitin-binding domains UDM1/2 to K63 ubiquitin chains...
December 2023: Biochemistry. Biokhimii︠a︡
https://read.qxmd.com/read/38461237/strategies-for-labelling-of-exogenous-and-endogenous-extracellular-vesicles-and-their-application-for-in-vitro-and-in-vivo-functional-studies
#35
REVIEW
Marie Boudna, Andres Delgado Campos, Petra Vychytilova-Faltejskova, Tana Machackova, Ondrej Slaby, Kamila Souckova
This review presents a comprehensive overview of labelling strategies for endogenous and exogenous extracellular vesicles, that can be utilised both in vitro and in vivo. It covers a broad spectrum of approaches, including fluorescent and bioluminescent labelling, and provides an analysis of their applications, strengths, and limitations. Furthermore, this article presents techniques that use radioactive tracers and contrast agents with the ability to track EVs both spatially and temporally. Emphasis is also placed on endogenous labelling mechanisms, represented by Cre-lox and CRISPR-Cas systems, which are powerful and flexible tools for real-time EV monitoring or tracking their fate in target cells...
March 9, 2024: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/38460550/monitoring-staphylococcus-aureus-nasal-colonization-murine-model-using-a-bioluminescent-methicillin-resistant-s-aureus-mrsa
#36
JOURNAL ARTICLE
Juliana G da Silva, Juliana Pc Boechat, Bruno Dj Silva, Rodrigo Müller, José Pm Senna
Staphylococcus aureus nasal carriage is considered a risk factor for infections, and the development of nasal decolonization strategies is highly relevant. Despite they are not naturally colonized by Staphylococcus , mice are a good model for S. aureus nasal colonization. Murine models are easy to manipulate, and inter-laboratory reproducibility makes them suitable for nasal colonization studies. Strategies using bioluminescent bacteria allow for the monitoring of infection over time without the need to sacrifice animals for bacterial quantification...
March 9, 2024: Laboratory Animals
https://read.qxmd.com/read/38458750/function-of-mast-cell-and-bile-cholangiocarcinoma-interplay-in-cholangiocarcinoma-microenvironment
#37
JOURNAL ARTICLE
Anda Shi, Zengli Liu, Zhongqi Fan, Kangshuai Li, Xingkai Liu, Yongchang Tang, Jiaming Hu, Xingyong Li, Lizhuang Shu, Liming Zhao, Lingling Huang, Zhiyue Zhang, Guoyue Lv, Zongli Zhang, Yunfei Xu
OBJECTIVE: The correlation between cholangiocarcinoma (CCA) progression and bile is rarely studied. Here, we aimed to identify differential metabolites in benign and malignant bile ducts and elucidate the generation, function and degradation of bile metabolites. DESIGN: Differential metabolites in the bile from CCA and benign biliary stenosis were identified by metabonomics. Biliary molecules able to induce mast cell (MC) degranulation were revealed by in vitro and in vivo experiments, including liquid chromatography-mass spectrometry (MS)/MS and bioluminescence resonance energy transfer assays...
March 8, 2024: Gut
https://read.qxmd.com/read/38458258/low-dose-radiotherapy-synergizes-with-irgd-anticd3-modified-t-cells-by-facilitating-t-cell-infiltration
#38
JOURNAL ARTICLE
Shujuan Zhou, Mei Zhu, Xiao Wei, Peiyuan Mu, Lijun Shen, Yan Wang, Juefeng Wan, Hui Zhang, Fan Xia, Zhen Zhang
BACKGROUND AND PURPOSE: Poor penetration of transferred T cells represents a critical factor impeding the development of adoptive cell therapy in solid tumors. We demonstrated that iRGD-antiCD3 modification promoted both T cell infiltration and activation in our previous work. Interest in low-dose radiotherapy has recently been renewed due to its immuno-stimulatory effects including T cell recruitment. This study aims to explore the synergistic effects between low-dose radiotherapy and iRGD-antiCD3-modified T cells...
March 6, 2024: Radiotherapy and Oncology
https://read.qxmd.com/read/38457503/a-hybrid-pathway-for-self-sustained-luminescence
#39
JOURNAL ARTICLE
Kseniia A Palkina, Tatiana A Karataeva, Maxim M Perfilov, Liliia I Fakhranurova, Nadezhda M Markina, Louisa Gonzalez Somermeyer, Elena Garcia-Perez, Marta Vazquez-Vilar, Marta Rodriguez-Rodriguez, Victor Vazquez-Vilriales, Ekaterina S Shakhova, Tatiana Mitiouchkina, Olga A Belozerova, Sergey I Kovalchuk, Anna Alekberova, Alena K Malyshevskaia, Evgenia N Bugaeva, Elena B Guglya, Anastasia Balakireva, Nikita Sytov, Anastasia Bezlikhotnova, Daria I Boldyreva, Vladislav V Babenko, Fyodor A Kondrashov, Vladimir V Choob, Diego Orzaez, Ilia V Yampolsky, Alexander S Mishin, Karen S Sarkisyan
The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis-a step catalyzed by a large fungal polyketide synthase that requires a posttranslational modification for activity. Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more compact, not dependent on availability of machinery for posttranslational modifications, and confers autonomous bioluminescence in yeast, mammalian, and plant hosts...
March 8, 2024: Science Advances
https://read.qxmd.com/read/38453673/amino-acid-encoded-bioinspired-supramolecular-self-assembly-of-multimorphological-nanocarriers
#40
JOURNAL ARTICLE
Yifan Huang, Guokun Yang, Zian Yu, Tong Tong, Yan Huang, Qianzijing Zhang, Yajian Hong, Jun Jiang, Guozhen Zhang, Yue Yuan
Supramolecular self-assembly has emerged as an efficient tool to construct well-organized nanostructures for biomedical applications by small organic molecules. However, the physicochemical properties of self-assembled nanoarchitectures are greatly influenced by their morphologies, mechanical properties, and working mechanisms, making it challenging to design and screen ideal building blocks. Herein, using a biocompatible firefly-sourced click reaction between the cyano group of 2-cyano-benzothiazole (CBT) and the 1,2-aminothiol group of cysteine (Cys), an amino-acid-encoded supramolecular self-assembly platform Cys(SEt)-X-CBT (X represents any amino acid) is developed to incorporate both covalent and noncovalent interactions for building diverse morphologies of nanostructures with bioinspired response mechanism, providing a convenient and rapid strategy to construct site-specific nanocarriers for drug delivery, cell imaging, and enzyme encapsulation...
March 7, 2024: Small
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