Kseniia A Palkina, Tatiana A Karataeva, Maxim M Perfilov, Liliia I Fakhranurova, Nadezhda M Markina, Louisa Gonzalez Somermeyer, Elena Garcia-Perez, Marta Vazquez-Vilar, Marta Rodriguez-Rodriguez, Victor Vazquez-Vilriales, Ekaterina S Shakhova, Tatiana Mitiouchkina, Olga A Belozerova, Sergey I Kovalchuk, Anna Alekberova, Alena K Malyshevskaia, Evgenia N Bugaeva, Elena B Guglya, Anastasia Balakireva, Nikita Sytov, Anastasia Bezlikhotnova, Daria I Boldyreva, Vladislav V Babenko, Fyodor A Kondrashov, Vladimir V Choob, Diego Orzaez, Ilia V Yampolsky, Alexander S Mishin, Karen S Sarkisyan
The fungal bioluminescence pathway can be reconstituted in other organisms allowing luminescence imaging without exogenously supplied substrate. The pathway starts from hispidin biosynthesis-a step catalyzed by a large fungal polyketide synthase that requires a posttranslational modification for activity. Here, we report identification of alternative compact hispidin synthases encoded by a phylogenetically diverse group of plants. A hybrid bioluminescence pathway that combines plant and fungal genes is more compact, not dependent on availability of machinery for posttranslational modifications, and confers autonomous bioluminescence in yeast, mammalian, and plant hosts...
March 8, 2024: Science Advances