keyword
https://read.qxmd.com/read/38482450/overexpression-of-klhl22-correlates-with-poor-prognosis-in-patients-with-triple-negative-breast-cancer
#1
JOURNAL ARTICLE
Tianzhi Zhang, Jiani Liu, Jin Wang, Chao Zhang
BACKGROUND: Kelch-like family member 22 ( KLHL22 ) is a protein-coding gene that is responsible for several Mendelian diseases and has been reported to promote tumorigenesis and aging. The purpose of this study was to investigate its expression in triple-negative breast cancer (TNBC) and its prognostic significance. METHODS: Immunohistochemistry (IHC) was performed to examine the expression levels of KLHL22 in 146 patients with TNBC. The Chi-squared test was used to analyze the correlations between KLHL22 expression level and clinicopathological features, and the Kaplan-Meier survival analysis and Cox multivariate regression model were used to analyze the prognostic significance of KLHL22 in patients with TNBC...
February 29, 2024: Translational Cancer Research
https://read.qxmd.com/read/37922866/cullin-g-glutamate-dehydrogenase-insights-into-multivalent-crl3-klhl22-substrate-recognition
#2
JOURNAL ARTICLE
David Schwefel
Substrate specificity is central to the regulation of cellular ubiquitylation. In this issue of Structure, Teng et al. employ biochemistry and cryo-EM single-particle reconstruction to clarify the intricate interaction of the dimeric CRL3KLHL22 E3 ligase assembly with a hexameric substrate and its possible implications for metabolic adaptation and oncogenesis.
November 2, 2023: Structure
https://read.qxmd.com/read/37788672/cryo-em-structure-of-the-klhl22-e3-ligase-bound-to-an-oligomeric-metabolic-enzyme
#3
JOURNAL ARTICLE
Fei Teng, Yang Wang, Ming Liu, Shuyun Tian, Goran Stjepanovic, Ming-Yuan Su
CULLIN-RING ligases constitute the largest group of E3 ubiquitin ligases. While some CULLIN family members recruit adapters before engaging further with different substrate receptors, homo-dimeric BTB-Kelch family proteins combine adapter and substrate receptor into a single polypeptide for the CULLIN3 family. However, the entire structural assembly and molecular details have not been elucidated to date. Here, we present a cryo-EM structure of the CULLIN3RBX1 in complex with Kelch-like protein 22 (KLHL22) and a mitochondrial glutamate dehydrogenase complex I (GDH1) at 3...
September 22, 2023: Structure
https://read.qxmd.com/read/36440598/fine-tuning-of-mtor-signaling-by-the-ube4b-klhl22-e3-ubiquitin-ligase-cascade-in-brain-development
#4
JOURNAL ARTICLE
Xiangxing Kong, Xin Shu, Jiachuan Wang, Dandan Liu, Yingchun Ni, Weiqi Zhao, Lebo Wang, Zhihua Gao, Jiadong Chen, Bing Yang, Xing Guo, Zhiping Wang
Spatiotemporal regulation of the mechanistic target of rapamycin (mTOR) pathway is pivotal for establishment of brain architecture. Dysregulation of mTOR signaling is associated with a variety of neurodevelopmental disorders (NDDs). Here, we discover that the UBE4B-KLHL22 E3 ubiquitin ligase cascade regulates mTOR activity in neurodevelopment. In a mouse model with UBE4B conditionally deleted in the nervous system, animals display severe growth defects, spontaneous seizures, and premature death. Loss of UBE4B in the brains of mutant mice results in depletion of neural precursor cells (NPCs) and impairment of neurogenesis...
November 28, 2022: Development
https://read.qxmd.com/read/36394357/xaf1-prevents-hyperproduction-of-type-i-interferon-upon-viral-infection-by-targeting-irf7
#5
JOURNAL ARTICLE
Bao-Qin Liu, Rong-Bei Liu, Wen-Ping Li, Xin-Tao Mao, Yi-Ning Li, Tao Huang, Hao-Li Wang, Hao-Tian Chen, Jiang-Yan Zhong, Bing Yang, Ren-Jie Chai, Qian Cao, Jin Jin, Yi-Yuan Li
Interferon regulatory factor (IRF) 3 and IRF7 are master regulators of type I interferon (IFN-I)-dependent antiviral innate immunity. Upon viral infection, a positive feedback loop is formed, wherein IRF7 promotes further induction of IFN-I in the later stage. Thus, it is critical to maintain a suitably low level of IRF7 to avoid the hyperproduction of IFN-I. In this study, we find that early expression of IFN-I-dependent STAT1 promotes the expression of XAF1 and that XAF1 is associated specifically with IRF7 and inhibits the activity of XIAP...
November 17, 2022: EMBO Reports
https://read.qxmd.com/read/35330733/targeted-exome-sequencing-of-genes-involved-in-rare-cnvs-in-early-onset-severe-obesity
#6
JOURNAL ARTICLE
Petra Loid, Minna Pekkinen, Taina Mustila, Päivi Tossavainen, Heli Viljakainen, Anna Lindstrand, Outi Mäkitie
Context: Rare copy number variants (CNVs) have been associated with the development of severe obesity. However, the potential disease-causing contribution of individual genes within the region of CNVs is often not known. Objective: Screening of rare variants in genes involved in CNVs in Finnish patients with severe early-onset obesity to find candidate genes linked to severe obesity. Methods: Custom-made targeted exome sequencing panel to search for rare (minor allele frequency <0.1%) variants in genes affected by previously identified CNVs in 92 subjects (median age 14 years) with early-onset severe obesity (median body mass index (BMI) Z-score + 4...
2022: Frontiers in Genetics
https://read.qxmd.com/read/33665188/genome-wide-analysis-of-differentially-expressed-mirnas-and-their-associated-regulatory-networks-in-lenses-deficient-for-the-congenital-cataract-linked-tudor-domain-containing-protein-tdrd7
#7
JOURNAL ARTICLE
Deepti Anand, Salma Al Saai, Sanjaya K Shrestha, Carrie E Barnum, Shinichiro Chuma, Salil A Lachke
Mutations/deficiency of TDRD7 , encoding a tudor domain protein involved in post-transcriptional gene expression control, causes early onset cataract in humans. While Tdrd7 is implicated in the control of key lens mRNAs, the impact of Tdrd7 deficiency on microRNAs (miRNAs) and how this contributes to transcriptome misexpression and to cataracts, is undefined. We address this critical knowledge-gap by investigating Tdrd7 -targeted knockout ( Tdrd7-/- ) mice that exhibit fully penetrant juvenile cataracts. We performed Affymetrix miRNA 3...
2021: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/33259306/an-enhanced-random-forests-approach-to-predict-heart-failure-from-small-imbalanced-gene-expression-data
#8
JOURNAL ARTICLE
Davide Chicco, Luca Oneto
Myocardial infarctions and heart failure are the cause of more than 17 million deaths annually worldwide. ST-segment elevation myocardial infarctions (STEMI) require timely treatment, because delays of minutes have serious clinical impacts. Machine learning can provide alternative ways to predict heart failure and identify genes involved in heart failure. For these scopes, we applied a Random Forests classifier enhanced with feature elimination to microarray gene expression of 111 patients diagnosed with STEMI, and measured the classification performance through standard metrics such as the Matthews correlation coefficient (MCC) and area under the receiver operating characteristic curve (ROC AUC)...
November 2021: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://read.qxmd.com/read/33109719/klhl22-maintains-pd-1-homeostasis-and-prevents-excessive-t-cell-suppression
#9
JOURNAL ARTICLE
Xiao Albert Zhou, Jiadong Zhou, Long Zhao, Guihui Yu, Jun Zhan, Chanyi Shi, Ruoshi Yuan, Yan Wang, Changfeng Chen, Wenjia Zhang, Donghao Xu, Yingjiang Ye, Weibin Wang, Zhanlong Shen, Wei Wang, Jiadong Wang
Aberrant programmed cell death protein 1 (PD-1) expression on the surface of T cells is known to inhibit T cell effector activity and to play a pivotal role in tumor immune escape; thus, maintaining an appropriate level of PD-1 expression is of great significance. We identified KLHL22, an adaptor of the Cul3-based E3 ligase, as a major PD-1-associated protein that mediates the degradation of PD-1 before its transport to the cell surface. KLHL22 deficiency leads to overaccumulation of PD-1, which represses the antitumor response of T cells and promotes tumor progression...
November 10, 2020: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/32547233/klhl22-regulates-the-emt-and-proliferation-in-colorectal-cancer-cells-in-part-via-the-wnt-%C3%AE-catenin-signaling-pathway
#10
JOURNAL ARTICLE
Yi Song, Huiping Yuan, Jia Wang, Yuhe Wu, Yuhong Xiao, Shengxun Mao
BACKGROUND: Colorectal cancer (CRC) is one of the most common aggressive malignancies. KLHL22 functions as a tumor suppressor, and previous findings have demonstrated that KLHL22 can regulate the development of breast cancer and CRC. However, few studies have investigated the role of KLHL22 in CRC cell epithelial-to-mesenchymal transition (EMT) and proliferation. The current study aimed to detect the role of KLHL22 in CRC cell proliferation and EMT and to elucidate the probable molecular mechanisms through which KLHL22 is involved with these processes...
2020: Cancer Management and Research
https://read.qxmd.com/read/32484697/klhl22-promotes-malignant-melanoma-growth-in-vitro-and-in-vivo-by-activating-the-pi3k-akt-mtor-signaling-pathway
#11
JOURNAL ARTICLE
X R Liu, W Wang, H M Li
The kelch like family member 22 (KLHL22) is a member of the KLHL (Kelch-like) gene family, which was involved in the progression of breast cancer. However, its role remains unclear in malignant melanoma (MM). Our study found that KLHL22 expression was upregulated in human MM tissues. Regarding the functional analysis for KLHL22 in the progression of MM cells, we demonstrated that overexpression of KLHL22 could promote MM cell growth in vitro. Vice versa, knockdown of KLHL22 could suppress the proliferation of MM cells...
September 2020: Neoplasma
https://read.qxmd.com/read/30614210/atypical-nested-22q11-2-duplications-between-lcr22b-and-lcr22d-are-associated-with-neurodevelopmental-phenotypes-including-autism-spectrum-disorder-with-incomplete-penetrance
#12
JOURNAL ARTICLE
Karen J Woodward, Julie Stampalia, Hannah Vanyai, Hashika Rijhumal, Kim Potts, Fiona Taylor, Joanne Peverall, Tanya Grumball, Soruba Sivamoorthy, Hamid Alinejad-Rokny, John Wray, Andrew Whitehouse, Lakshmi Nagarajan, Jacqueline Scurlock, Sabine Afchani, Matthew Edwards, Ashleigh Murch, John Beilby, Gareth Baynam, Cathy Kiraly-Borri, Fiona McKenzie, Julian I T Heng
BACKGROUND: Chromosome 22q11.2 is susceptible to genomic rearrangements and the most frequently reported involve deletions and duplications between low copy repeats LCR22A to LCR22D. Atypical nested deletions and duplications are rarer and can provide a valuable opportunity to investigate the dosage effects of a smaller subset of genes within the 22q11.2 genomic disorder region. METHODS: We describe thirteen individuals from six families, each with atypical nested duplications within the central 22q11...
January 4, 2019: Molecular Genetics & Genomic Medicine
https://read.qxmd.com/read/29925309/accurate-diagnosis-of-spinal-muscular-atrophy-and-22q11-2-deletion-syndrome-using-limited-deoxynucleotide-triphosphates-and-high-resolution-melting
#13
JOURNAL ARTICLE
Xiaoqing Zhang, Bo Wang, Lichen Zhang, Guoling You, Robert A Palais, Luming Zhou, Qihua Fu
BACKGROUND: Copy number variation (CNV) has been implicated in the genetics of multiple human diseases. Spinal muscular atrophy (SMA) and 22q11.2 deletion syndrome (22q11.2DS) are two of the most common diseases which are caused by DNA copy number variations. Genetic diagnostics for these conditions would be enhanced by more accurate and efficient methods to detect the relevant CNVs. METHODS: Competitive PCR with limited deoxynucleotide triphosphates (dNTPs) and high-resolution melting (HRM) analysis was used to detect 22q11...
June 20, 2018: BMC Genomics
https://read.qxmd.com/read/29802162/klhl22-promotes-mtorc1-activation-and-breast-tumorigenesis
#14
JOURNAL ARTICLE
(no author information available yet)
Amino acids promote CUL3-KLHL22-mediated DEPDC5 degradation to relieve mTORC1 inhibition.
July 2018: Cancer Discovery
https://read.qxmd.com/read/29769719/klhl22-activates-amino-acid-dependent-mtorc1-signalling-to-promote-tumorigenesis-and-ageing
#15
JOURNAL ARTICLE
Jie Chen, Yuhui Ou, Yanyan Yang, Wen Li, Ye Xu, Yuntao Xie, Ying Liu
The mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of cell growth that responds to a diverse set of environmental cues, including amino acids1,2 . Deregulation of mTORC1 has been linked with metabolic diseases, cancer and ageing2-4 . In response to amino acids, mTORC1 is recruited by the Rag GTPases to the lysosome, its site of activation5,6 . The GATOR1 complex, consisting of DEPDC5, NPRL3 and NPRL2, displays GAP activity to inactivate Rag GTPases under amino-acid-deficient conditions 7 ...
May 2018: Nature
https://read.qxmd.com/read/24912477/exome-sequencing-of-hepatoblastoma-reveals-novel-mutations-and-cancer-genes-in-the-wnt-pathway-and-ubiquitin-ligase-complex
#16
JOURNAL ARTICLE
Deshui Jia, Rui Dong, Ying Jing, Dan Xu, Qifeng Wang, Lei Chen, Qigen Li, Yuping Huang, Yuannv Zhang, Zhenfeng Zhang, Li Liu, Shan Zheng, Qiang Xia, Hongyang Wang, Kuiran Dong, Xianghuo He
UNLABELLED: Hepatoblastoma (HB) is the most common primary liver tumor in children. Mutations in the β-catenin gene that lead to constitutive activation of the Wnt pathway have been detected in a large proportion of HB tumors. To identify novel mutations in HB, we performed whole-exome sequencing of six paired HB tumors and their corresponding lymphocytes. This identified 24 somatic nonsynonymous mutations in 21 genes, many of which were novel, including three novel mutations targeting the CTNNB1 (G512V) and CAPRIN2 (R968H/S969C) genes in the Wnt pathway, and genes previously shown to be involved in the ubiquitin ligase complex (SPOP, KLHL22, TRPC4AP, and RNF169)...
November 2014: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://read.qxmd.com/read/24067371/cul3-and-protein-kinases-insights-from-plk1-klhl22-interaction
#17
JOURNAL ARTICLE
Thibaud Metzger, Charlotte Kleiss, Izabela Sumara
Posttranslational mechanisms drive fidelity of cellular processes. Phosphorylation and ubiquitination of substrates represent very common, covalent, posttranslational modifications and are often co-regulated. Phosphorylation may play a critical role both by directly regulating E3-ubiquitin ligases and/or by ensuring specificity of the ubiquitination substrate. Importantly, many kinases are not only critical regulatory components of these pathways but also represent themselves the direct ubiquitination substrates...
July 15, 2013: Cell Cycle
https://read.qxmd.com/read/23907128/regulating-plk1-dynamics-by-cullin3-klhl22-mediated-ubiquitylation
#18
EDITORIAL
Jochen Beck, Matthias Peter
No abstract text is available yet for this article.
August 15, 2013: Cell Cycle
https://read.qxmd.com/read/23797583/cul3-and-protein-kinases-insights-from-plk1-klhl22-interaction
#19
Thibaud Metzger, Charlotte Kleiss, Izabela Sumara
Posttranslational mechanisms drive fidelity of cellular processes. Phosphorylation and ubiquitination of substrates represent very common, covalent, posttranslational modifications and are often co-regulated. Phosphorylation may play a critical role both by directly regulating E3-ubiquitin ligases and/or by ensuring specificity of the ubiquitination substrate. Importantly, many kinases are not only critical regulatory components of these pathways but also represent themselves the direct ubiquitination substrates...
June 24, 2013: Cell Cycle
https://read.qxmd.com/read/23548928/cullin-plk1-from-kinetochores
#20
COMMENT
Colleen A McGourty, Michael Rape
To ensure proper attachment of all chromosomes to the spindle, PLK1 has to associate with kinetochores during prometaphase and must be released from these sites before sister chromatid separation can begin. The monoubiquitylation of PLK1 by the ubiquitin ligase CUL3-KLHL22 is now identified as a critical step in promoting the release of PLK1 from kinetochores, pushing non-proteolytic ubiquitylation into the limelight of cell division research.
April 2013: Nature Cell Biology
keyword
keyword
164431
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.