keyword
https://read.qxmd.com/read/38531825/whole-blood-storage-duration-alters-fibrinogen-levels-and-thrombin-formation
#1
JOURNAL ARTICLE
Ryan Chae, Christopher Nguyen, Chad Archdeacon, Lindsey Wattley, Stephanie Sisak, Adam Price, Emma Perez, Rebecca Schuster, Alex Lentsch, Charles Caldwell, Michael Goodman, Timothy Pritts
INTRODUCTION: Whole blood resuscitation for hemorrhagic shock in trauma represents an opportunity to correct coagulopathy in trauma while also supplying red blood cells. The production of microvesicles in stored whole blood and their effect on its hemostatic parameters have not been described in previous literature. We hypothesized that microvesicles in aged stored whole blood are procoagulant and increase thrombin production via phosphatidylserine. METHODS: Whole blood was obtained from male C57BL/6 male mice and stored in anticoagulant solution for up to 10 days...
March 27, 2024: Journal of Trauma and Acute Care Surgery
https://read.qxmd.com/read/38527011/natalizumab-promotes-anti-inflammatory-and-repair-effects-in-multiple-sclerosis
#2
JOURNAL ARTICLE
Ragnhild Reehorst Lereim, Petra Nytrova, Astrid Guldbrandsen, Eva Kubala Havrdova, Kjell-Morten Myhr, Harald Barsnes, Frode S Berven
BACKGROUND: Multiple sclerosis is an inflammatory and degenerative disease of the central nervous system leading to demyelination and axonal loss. Relapsing-remitting multiple sclerosis (RRMS) is commonly treated by anti-inflammatory drugs, where one of the most effective drugs to date is the monoclonal antibody natalizumab. METHODS: The cerebrospinal fluid (CSF) proteome was analyzed in 56 patients with RRMS before and after natalizumab treatment, using label-free mass spectrometry and a subset of the changed proteins were verified by parallel reaction monitoring in a new cohort of 20 patients, confirming the majority of observed changes...
2024: PloS One
https://read.qxmd.com/read/38465331/common-proteins-analysis-of-different-mammals-mature-milk-by-4d-label-free
#3
JOURNAL ARTICLE
Rui Chen, Yinggang Sun, Yanzhi Wu, Yanjun Qiao, Qiu Zhang, Qian Li, Xiaowei Wang, Yuan Pan, Siyi Li, Yining Liu, Zeying Wang
The milk proteins from samples of 13 different animals were identified utilizing 4D-Label-Free proteomics technology, leading to the identification of a substantial number of proteins. Among the various samples, Chinese people (CHP) milk proteins exhibited the highest count, with 1149 distinct proteins. Simultaneously, we identified common proteins present in these animal milk. It's notable presence in goat milk contributes to enhancing infant infection resistance, showcasing the beneficial role of lactoperoxidase...
June 30, 2024: Food chemistry: X
https://read.qxmd.com/read/38444077/oxldl-enhances-procoagulant-activity-of-endothelial-cells-by-tmem16f-mediated-phosphatidylserine-exposure
#4
JOURNAL ARTICLE
Meishan Yan, Zelong Wang, Yao An, Zhanni Li, Yun Li, Hongyu Zhang, Caixia Li, Lifeng Wang, Li Chen, Chao Gao, Dongsheng Wang, Chunyan Gao
Oxidized low-density lipoprotein (oxLDL), a key component in atherosclerosis and hyperlipidemia, is a risk factor for atherothrombosis in dyslipidemia, yet its mechanism is poorly understood. In this study, we used oxLDL-induced human aortic endothelial cells (HAECs) and high-fat diet (HFD)-fed mice as a hyperlipidemia model. Phosphatidylserine (PS) exposure, cytosolic Ca2+ , reactive oxygen species (ROS), and lipid peroxidation were measured by flow cytometer. TMEM16F expression was detected by immunofluorescence, western blot, and reverse transcription polymerase chain reaction...
March 5, 2024: Cell Biology International
https://read.qxmd.com/read/38387190/brain-derived-extracellular-vesicles-mediate-systemic-coagulopathy-and-inflammation-after-traumatic-brain-injury
#5
JOURNAL ARTICLE
Fanjian Li, Lei Li, Ruilong Peng, Chuan Liu, Xiao Liu, Yafan Liu, Cong Wang, Jianye Xu, Qiaoling Zhang, Guili Yang, Ying Li, FangLian Chen, Shenghui Li, Weiyun Cui, Li Liu, Xin Xu, Shu Zhang, Zilong Zhao, Jianning Zhang
Traumatic brain injury (TBI) can induce systemic coagulopathy and inflammation, thereby increasing the risk of mortality and disability. However, the mechanism causing systemic coagulopathy and inflammation following TBI remains unclear. In prior research, we discovered that brain-derived extracellular vesicles (BDEVs), originating from the injured brain, can activate the coagulation cascade and inflammatory cells. In this study, we primarily investigated how BDEVs affect systemic coagulopathy and inflammation in peripheral circulation...
February 21, 2024: International Immunopharmacology
https://read.qxmd.com/read/38380806/increased-circulating-platelet-derived-extracellular-vesicles-in-severe-covid-19-disease
#6
JOURNAL ARTICLE
Tuukka Helin, Mari Palviainen, Marja Lemponen, Katariina Maaninka, Pia Siljander, Lotta Joutsi-Korhonen
Coagulation disturbances are major contributors to COVID-19 pathogenicity, but limited data exist on the involvement of extracellular vesicles (EVs) and residual cells (RCs). Fifty hospitalized COVID-19 patients stratified by their D-dimer levels into high (>1.5 mg/L, n  = 15) or low (≤1.5 mg/l, n  = 35) and 10 healthy controls were assessed for medium-sized EVs (mEVs; 200-1000 nm) and large EVs/RCs (1000-4000 nm) by high sensitivity flow cytometry...
December 2024: Platelets
https://read.qxmd.com/read/38374450/an-omics-based-machine-learning-approach-to-predict-diabetes-progression-a-rhapsody-study
#7
JOURNAL ARTICLE
Roderick C Slieker, Magnus Münch, Louise A Donnelly, Gerard A Bouland, Iulian Dragan, Dmitry Kuznetsov, Petra J M Elders, Guy A Rutter, Mark Ibberson, Ewan R Pearson, Leen M 't Hart, Mark A van de Wiel, Joline W J Beulens
AIMS/HYPOTHESIS: People with type 2 diabetes are heterogeneous in their disease trajectory, with some progressing more quickly to insulin initiation than others. Although classical biomarkers such as age, HbA1c and diabetes duration are associated with glycaemic progression, it is unclear how well such variables predict insulin initiation or requirement and whether newly identified markers have added predictive value. METHODS: In two prospective cohort studies as part of IMI-RHAPSODY, we investigated whether clinical variables and three types of molecular markers (metabolites, lipids, proteins) can predict time to insulin requirement using different machine learning approaches (lasso, ridge, GRridge, random forest)...
February 19, 2024: Diabetologia
https://read.qxmd.com/read/38344467/fluid-biomarkers-in-cerebral-amyloid-angiopathy
#8
REVIEW
Seyed Mehrdad Savar, Bin Ma, Eugene Hone, Farzana Jahan, Shaun Markovic, Steve Pedrini, Soudabeh Shemehsavar, Vandhana Easwaran, Kevin Taddei, Samantha Gardener, Jasmeer P Chhatwal, Ellis S van Etten, Matthias J P van Osch, Daniel Clarke, Anastazija Gnjec, Mark A van Buchem, Marieke J H Wermer, Graeme J Hankey, Steven M Greenberg, Ralph N Martins, Hamid R Sohrabi
Cerebral amyloid angiopathy (CAA) is a type of cerebrovascular disorder characterised by the accumulation of amyloid within the leptomeninges and small/medium-sized cerebral blood vessels. Typically, cerebral haemorrhages are one of the first clinical manifestations of CAA, posing a considerable challenge to the timely diagnosis of CAA as the bleedings only occur during the later disease stages. Fluid biomarkers may change prior to imaging biomarkers, and therefore, they could be the future of CAA diagnosis...
2024: Frontiers in Neuroscience
https://read.qxmd.com/read/38310527/serum-level-of-lactadherin-mfge8-in-inflamatory-bowel-disease
#9
JOURNAL ARTICLE
Kamil Kozioł, Danuta Owczarek, Dorota Cibor, Małgorzata Zwolińska-Wcisło
OBJECTIVE: Evaluation of serum lactadherin level, its correlation with disease activity and certain biochemical parameters in IBD patients. METHODS: The study involved adult IBD patients, comprising 50 with ulcerative colitis (UC), 68 with Crohn's disease (CD), and 29 healthy controls. RESULTS: The MFGE8 median concentration was significantly higher in UC versus controls (1914.54 vs. 1392.21; p = 0.017), but not in CD. The median MFGE8 levels in UC and CD patient groups didn't significantly differ...
October 30, 2023: Folia Medica Cracoviensia
https://read.qxmd.com/read/38233928/neutrophil-extracellular-traps-mediated-by-platelet-microvesicles-promote-thrombosis-and-brain-injury-in-acute-ischemic-stroke
#10
JOURNAL ARTICLE
Xin Gao, Xinyi Zhao, Jiacheng Li, Chang Liu, Wenqiang Li, Junjie Zhao, Zhixi Li, Nan Wang, Fang Wang, Jiawei Dong, Xiuwei Yan, Jiheng Zhang, Xueyan Hu, Jiaqi Jin, Ge Mang, Ruishuang Ma, Shaoshan Hu
AIMS: Neutrophil extracellular traps (NETs) have been implicated in thrombotic diseases. There is no definitive explanation for how NETs form during acute ischemic strokes (AIS). The purpose of our study was to investigate the potential mechanism and role of NETs formation in the AIS process. METHODS: As well as 45 healthy subjects, 45 patients with AIS had ELISA tests performed to detect NET markers. Expression of high-mobility group box 1 (HMGB1) on platelet microvesicles (PMVs) was analyzed by flow cytometry in healthy subjects and AIS patients' blood samples...
January 17, 2024: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/38173019/lactadherin-immunoblockade-in-small-extracellular-vesicles-inhibits-sev-mediated-increase-of-pro-metastatic-capacities
#11
JOURNAL ARTICLE
Eduardo Durán-Jara, Matías Del Campo, Valentina Gutiérrez, Ignacio Wichmann, César Trigo, Marcelo Ezquer, Lorena Lobos-González
BACKGROUND: Tumor-derived small extracellular vesicles (sEVs) can promote tumorigenic and metastatic capacities in less aggressive recipient cells mainly through the biomolecules in their cargo. However, despite recent advances, the specific molecules orchestrating these changes are not completely defined. Lactadherin is a secreted glycoprotein typically found in the milk fat globule membrane. Its overexpression has been associated with increased tumorigenesis and metastasis in breast cancer (BC) and other tumors...
January 3, 2024: Biological Research
https://read.qxmd.com/read/38141925/remodelling-of-the-cardiac-extracellular-matrix-proteome-during-chronological-and-pathological-ageing
#12
JOURNAL ARTICLE
Deolinda Santinha, Andreia Vilaça, Luís Estronca, Svenja C Schüler, Catherine Bartoli, Annachiara De Sandre-Giovannoli, Arnaldo Figueiredo, Maximillian Quaas, Tilo Pompe, Alessandro Ori, Lino Ferreira
Impaired extracellular matrix (ECM) remodelling is a hallmark of many chronic inflammatory disorders that can lead to cellular dysfunction, ageing, and disease progression. The ECM of the aged heart and its effects on cardiac cells during chronological and pathological ageing are poorly understood across species. For this purpose, we first used mass spectrometry-based proteomics to quantitatively characterize age-related remodelling of the left ventricle (LV) of mice and humans during chronological and pathological (Hutchinson-Gilford progeria syndrome (HGPS)) ageing...
December 21, 2023: Molecular & Cellular Proteomics: MCP
https://read.qxmd.com/read/38103786/phosphatidylthreonine-is-a-procoagulant-lipid-detected-in-human-blood-and-elevated-in-coronary-artery-disease
#13
JOURNAL ARTICLE
Ali A Hajeyah, Majd B Protty, Divyani Paul, Daniela Costa, Nader Omidvar, Bethan Morgan, Yugo Iwasaki, Beth McGill, P Vincent Jenkins, Zaheer Yousef, Keith Allen-Redpath, Shin Soyama, Anirban Choudhury, Rito Mitra, Parveen Yaqoob, James H Morrissey, Peter W Collins, Valerie B O'Donnell
Aminophospholipids (aPL) such as phosphatidylserine are essential for supporting the activity of coagulation factors, circulating platelets, and blood cells. Phosphatidylthreonine (PT) is an aminophospholipid previously reported in eukaryotic parasites and animal cell cultures, but not yet in human tissues. Here, we evaluated whether PT is present in blood cells and characterized its ability to support coagulation. Several PT molecular species were detected in human blood, washed platelets, extracellular vesicles, and isolated leukocytes from healthy volunteers using liquid chromatography-tandem mass spectrometry...
December 14, 2023: Journal of Lipid Research
https://read.qxmd.com/read/37747791/lactadherin-s-multistate-binding-predicts-stable-membrane-bound-conformations-of-factors-v-and-viii-s-c-domains
#14
JOURNAL ARTICLE
Kevin J Cheng, Ashley M De Lio, Riya Jain, Divyani Paul, James H Morrissey, Taras V Pogorelov
Protein binding to negatively charged lipids is essential for maintaining numerous vital cellular processes where its dysfunction can lead to various diseases. One such protein that plays a crucial role in this process is lactadherin, which competes with coagulation factors for membrane binding sites to regulate blood clotting. Despite identifying key binding regions of these proteins through structural and biochemical studies, models incorporating membrane dynamics are still lacking. In this study, we report on the multimodal binding of lactadherin and use it to gain insight into the binding mechanisms of its C domain homologs, factor V and factor VIII...
October 17, 2023: Biochemistry
https://read.qxmd.com/read/37725360/evaluation-of-the-differences-in-proteomics-of-high-quality-bovine-colostrum-and-low-quality-bovine-colostrum
#15
JOURNAL ARTICLE
Yiğit Kaçar, Hasan Batmaz
BACKGROUND: Although there are studies on colostrum and milk proteomics of different species in the literature, there is no published report about different quality bovine colostrums' proteomics. OBJECTIVES: The aim of this study was to compare the proteome content of high- and low-quality bovine colostrums for the first time. METHODS: Colostrum samples were collected from 32 Holstein cows from the same farm that had just calved. Brix% levels of colostrums were measured, and then, those with a Brix% value of ≥27% were classified as high-quality and those with a Brix% value of <22% as low-quality...
November 2023: Veterinary Medicine and Science
https://read.qxmd.com/read/37538497/plasma-extracellular-vesicle-test-sample-to-standardize-flow-cytometry-measurements
#16
JOURNAL ARTICLE
Britta Bettin, Edwin van der Pol, Rienk Nieuwland
BACKGROUND: Extracellular vesicles (EVs) in body fluids are explored as disease biomarkers, but EV concentrations measured by flow cytometers (FCMs) are incomparable. OBJECTIVES: To improve data comparability, new reference materials with physical properties resembling EVs and reference procedures are being developed. The validation of new reference materials and procedures requires biological test samples. We developed a human plasma EV test sample (PEVTES) that i) resembles subcellular particles in plasma, ii) is ready-to-use, iii) is flow cytometry-compatible, and iv) is stable...
May 2023: Research and Practice in Thrombosis and Haemostasis
https://read.qxmd.com/read/37501184/genetically-encoded-phosphatidylserine-biosensor-for-in-vitro-ex-vivo-and-in-vivo-labelling
#17
JOURNAL ARTICLE
Eimina Dirvelyte, Daina Bujanauskiene, Evelina Jankaityte, Neringa Daugelaviciene, Ugne Kisieliute, Igor Nagula, Rima Budvytyte, Urte Neniskyte
BACKGROUND: The dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is found preferentially in the inner leaflet of the plasma membrane. Disruption of this asymmetry leads to the exposure of phosphatidylserine on the cell surface and is associated with cell death, synaptic pruning, blood clotting and other cellular processes. Due to the role of phosphatidylserine in widespread cellular functions, an efficient phosphatidylserine probe is needed to study them...
July 27, 2023: Cellular & Molecular Biology Letters
https://read.qxmd.com/read/37308423/-effects-of-exhaustive-exercise-on-coagulation-state-in-rats
#18
JOURNAL ARTICLE
Yan-Shi Xia, Hong Gao, Dan-Wei Zhao
Objective: To investigate the effects of one-time exhaustive exercise on coagulation state in rats and its mechanism. Methods: Forty-eight SD rats were randomly divided into control group and exhaustive exercise group, 24 rats in each group. Rats in exhaustive exercise group were trained with treadmill training for 25~50 min at a time on non-slope treadmill and the initial speed of 5 m/min was uniformly accelerated to 25 m/min until the rats exhausted. Thrombelastography (TEG) was used to monitor the coagulation function of rats after training...
November 2022: Chinese Journal of Applied Physiology
https://read.qxmd.com/read/37163934/brain-derived-extracellular-vesicles-mediate-traumatic-brain-injury-associated-multi-organ-damage
#19
JOURNAL ARTICLE
Fanjian Li, Yafan Liu, Lei Li, Ruilong Peng, Cong Wang, Chuan Liu, Mingming Shi, Yiyao Cao, Yalong Gao, Hejun Zhang, Xilei Liu, Tuo Li, Haoran Jia, Xiaotian Li, Qiaoling Zhang, Zilong Zhao, Jianning Zhang
Traumatic brain injury (TBI) can negatively impact systemic organs, which can lead to more death and disability. However, the mechanism underlying the effect of TBI on systemic organs remains unclear. In previous work, we found that brain-derived extracellular vesicles (BDEVs) released from the injured brain can induce systemic coagulation with a widespread fibrin deposition in the microvasculature of the lungs, kidney, and heart in a mouse model of TBI. In this study, we investigated whether BDEVs can induce heart, lung, liver, and kidney injury in TBI mice...
May 1, 2023: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/37046617/microparticle-phosphatidylserine-mediates-coagulation-involvement-in-tumor-progression-and-metastasis
#20
REVIEW
Haijiao Jing, Xiaoming Wu, Mengqi Xiang, Chengyue Wang, Valerie A Novakovic, Jialan Shi
Tumor progression and cancer metastasis has been linked to the release of microparticles (MPs), which are shed upon cell activation or apoptosis and display parental cell antigens, phospholipids such as phosphatidylserine (PS), and nucleic acids on their external surfaces. In this review, we highlight the biogenesis of MPs as well as the pathophysiological processes of PS externalization and its involvement in coagulation activation. We review the available evidence, suggesting that coagulation factors (mainly tissue factor, thrombin, and fibrin) assist in multiple steps of tumor dissemination, including epithelial-mesenchymal transition, extracellular matrix remodeling, immune escape, and tumor angiogenesis to support the formation of the pre-metastatic niche...
March 24, 2023: Cancers
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