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Sp1 and cardiomyocytes

Zhao Li, Vineet Agrawal, Mohun Ramratnam, Ravi K Sharma, Stephen D'Auria, Abigail Sincoular, Margurite Jakubiak, Meredith L Music, William J Kutschke, Xueyin N Huang, Lindsey Gifford, Ferhaan Ahmad
Aims: We previously reported that sodium-dependent glucose cotransporter 1 (SGLT1) is highly expressed in cardiomyocytes, and is further upregulated in ischemia. This study aimed to determine the mechanisms by which SGLT1 contributes to ischemia/reperfusion (I/R) injury. Methods and Results: Mice with cardiomyocyte-specific knockdown of SGLT1 (TGSGLT1-DOWN) and wildtype controls were studied. In vivo, the LAD coronary artery was ligated for 30 min and reperfused for 48 h...
February 4, 2019: Cardiovascular Research
Ye Wang, Rongyi Cao, Wenwen Yang, Bangruo Qi
Cardiac hypertrophy (CH) has become a huge threat to human health. Recent years, long noncoding RNAs (lncRNAs) have been studied in human diseases, including CH. According to bioinformatics analysis, 10 lncRNAs possibly involved in the progression of CH were screened out. Among which, lncRNA SYNE1 antisense RNA 1 (SYNE1-AS1) could be upregulated by Angiotensin II (Ang-II) in cardiomyocytes. Thus, we chose SYNE1-AS1 to do further study. To identify the biological function of SYNE1-AS1 in CH, SYNE1-AS1 was silenced in Ang-II-induced cardiomyocytes...
January 16, 2019: Journal of Cellular Physiology
Lu Ding, Chengxin Gong, Jiani Zhao, Xingzi Liu, Tao Li, Shenqiang Rao, Shuo Wang, Yuanyuan Liu, Shanping Peng, Wen Xiao, Chaopeng Xiong, Rumeng Wang, Shangdong Liang, Hong Xu
Increasing evidence has suggested high-fat diet (HFD) is an independent risk factor for myocardial ischemia/reperfusion (MI/R) injury. Long noncoding RNAs (lncRNAs) recently attracted much attraction in the study of MI/R injury. However, the functional questions of specific lncRNAs in HFD-induced MI/R injury have not been well elucidated. Uc.48+ is a lncRNA from a transcribed ultraconserved region (T-UCR) of human, mouse, and rat genomes. Here, we explored the aggravating role of uc.48+and identified purinergic P2X7 receptor (P2X7R) as a downstream regulator of uc...
November 11, 2018: Journal of Cellular Physiology
Xinxing Wang, Shang Wang, Weili Liu, Tianhui Wang, Jing Wang, Xiujie Gao, Ruifeng Duan, Yingying Li, Lingling Pu, Bingnan Deng, Zhaoli Chen
TOMM40 is the channel-forming subunit of a translocase of the mitochondrial outer membrane (TOM) that is essential for protein transport into mitochondria. TOMM40 plays an important role in maintaining normal mitochondrial function. The correlation between occupational thermal exposure and mitochondria dysfunction has been demonstrated; however, nothing is known about the alteration and role of TOMM40 in response to environmental heat stress. In the present study, we showed that environmental thermal exposure upregulated microRNA miR-126, consequently reducing AU-rich element RNA-binding protein 1 (AUF1)-mediated SP1 mRNA degradation and increasing TOMM40 transcription, which in turn decreased the mitochondria membrane potential and apoptosis of cardiomyocytes...
October 5, 2018: Journal of Molecular and Cellular Cardiology
Simei Sun, Hae Jin Kee, Li Jin, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Myung Ho Jeong
We previously reported that gentisic acid (2,5-dihydroxybenzoic acid) is the third most abundant phenolic component of Dendropanax morbifera branch extracts. Here, we investigated its effects on cardiac hypertrophy and fibrosis in a mouse model of pressure overload and compared them to those of the beta blocker bisoprolol and calcium channel blocker diltiazem. Cardiac hypertrophy was induced in mice by transverse aortic constriction (TAC). Beginning 2 weeks after this procedure, the mice were given daily intraperitoneal injections of gentisic acid (100 mg/kg/d), bisoprolol (5 mg/kg/d) or diltiazem (10 mg/kg/d) for 3 weeks...
December 2018: Journal of Cellular and Molecular Medicine
Yan-Xia Lv, Sen Zhong, Hexin Tang, Bin Luo, Shao-Juan Chen, Long Chen, Fei Zheng, Lei Zhang, Lu Wang, Xing-Yuan Li, Yu-Wen Yan, Ya-Mu Pan, Miao Jiang, You-En Zhang, Lei Wang, Jian-Ye Yang, Ling-Yun Guo, Shi-You Chen, Jia-Ning Wang, Jun-Ming Tang
BACKGROUND/AIMS: Vagus nerve stimulation (VNS) suppresses arrhythmic activity and minimizes cardiomyocyte injury. However, how VNS affects angiogenesis/arteriogenesis in infarcted hearts, is poorly understood. METHODS: Myocardial infarction (MI) was achieved by ligation of the left anterior descending coronary artery (LAD) in rats. 7 days after LAD, stainless-steel wires were looped around the left and right vagal nerve in the neck for vagus nerve stimulation (VNS)...
2018: Cellular Physiology and Biochemistry
Simei Sun, Tianyi Li, Li Jin, Zhe Hao Piao, Bin Liu, Yuhee Ryu, Sin Young Choi, Gwi Ran Kim, Ji Eun Jeong, An Jin Wi, Song Ju Lee, Hae Jin Kee, Myung Ho Jeong
An extract of Dendropanax morbifera branch exerts antioxidant, anti-inflammatory, antithrombotic, and anticancer activities. The purpose of this study was to investigate the effect of the extract in isoproterenol-induced cardiac hypertrophy. Phalloidin staining showed that treatment with the extract dramatically prevents isoproterenol-induced H9c2 cell enlargement and the expression of cardiac hypertrophic marker genes, including atrial natriuretic peptide (ANP) and B-type brain natriuretic peptide (BNP). Further, pretreatment with the extract decreased isoproterenol-induced GATA4 and Sp1 expression in H9c2 cells...
2018: American Journal of Chinese Medicine
Shu-Bo Zhang, Tie-Jun Liu, Guo-Hua Pu, Bao-Yong Li, Xiao-Zeng Gao, Xiao-Liang Han
BACKGROUND/AIMS: Ischemic heart disease is a leading cause of death in cardiovascular diseases, and microRNAs (miRs) have been reported to be potential therapeutic targets in heart disease. Herein, this study aims to investigate the effects of microRNA (miR)-374 on myocardial ischemia-reperfusion (I/R) injury in rat models pretreated with sevoflurane by targeting SP1 through the PI3K/Akt pathway. METHODS: SD rats were grouped into sham, I/R and sevoflurane + I/R (sevoflurane preconditioning and I/R) groups...
2018: Cellular Physiology and Biochemistry
Kimiko Masuda, Hiroki Takanari, Masaki Morishima, FangFang Ma, Yan Wang, Naohiko Takahashi, Katsushige Ono
Men have shorter rate-corrected QT intervals (QTc) than women, especially at the period of adolescence or later. The aim of this study was to elucidate the long-term effects of testosterone on cardiac excitability parameters including electrocardiogram (ECG) and potassium channel current. Testosterone shortened QT intervals in ECG in castrated male rats, not immediately after, but on day 2 or later. Expression of Kv7.1 (KCNQ1) mRNA was significantly upregulated by testosterone in cardiomyocytes of male and female rats...
November 2018: Journal of Physiological Sciences: JPS
Beth A Rose, Tomohiro Yokota, Vishnu Chintalgattu, Shuxun Ren, Luisa Iruela-Arispe, Aarif Y Khakoo, Susumu Minamisawa, Yibin Wang
Stress-induced p38 mitogen-activated protein kinase (MAPK) activity is implicated in pathological remodeling in the heart. For example, constitutive p38 MAPK activation in cardiomyocytes induces pathological features, including myocyte hypertrophy, apoptosis, contractile dysfunction, and fetal gene expression. However, the physiological function of cardiomyocyte p38 MAPK activity in beneficial compensatory vascular remodeling is unclear. This report investigated the functional role and the underlying mechanisms of cardiomyocyte p38 MAPK activity in cardiac remodeling induced by chronic stress...
August 4, 2017: Journal of Biological Chemistry
Chen Chen, Shenglan Yang, Huaping Li, Zhongwei Yin, Jiahui Fan, Yanru Zhao, Wei Gong, Mengwen Yan, Dao Wen Wang
Multiple factors have been shown to promote the progression of diabetic cardiomyopathy. A link has previously been found between Mir30 and autophagy in cancer cells and in the heart, but the role of Mir30 in diabetic heart has not been studied. Using in vitro and in vivo approaches, we found that the depletion of Mir30c and induction of BECN1 enhanced autophagy in diabetic (db/db) hearts and in cardiomyocytes treated with the fatty acid palmitate. We verified that Mir30c repressed BECN1 expression by direct binding to the BECN1 3' UTRs...
June 16, 2017: Molecular Therapy. Nucleic Acids
Shyam Sundar Nandi, Paras Kumar Mishra
Hydrogen sulfide (H2S), a cardioprotective gas, is endogenously produced from homocysteine by cystathionine beta synthase (CBS) and cystathionine gamma lyase (CSE) enzymes. However, effect of H2S or homocysteine on CBS and CSE expression, and cross-talk between CBS and CSE are unclear. We hypothesize that homocysteine and H2S regulate CBS and CSE expressions in a dose dependent manner in cardiomyocytes, and CBS deficiency induces cardiac CSE expression. To test the hypothesis, we treated murine atrial HL1 cardiomyocytes with increasing doses of homocysteine or Na2S/GYY4137, a H2S donor, and measured the levels of CBS and CSE...
June 16, 2017: Scientific Reports
Zeng-Xiang Dong, Lin Wan, Ren-Jun Wang, Yuan-Qi Shi, Guang-Zhong Liu, Si-Jia Zheng, Hui-Ling Hou, Wei Han, Xin Hai
BACKGROUND/AIMS: Flavonol (-)-epicatechin (EPI) is present in high amounts in cocoa and tea products, and has been shown to exert beneficial effects on the cardiovascular system. However, the precise mechanism of EPI on cardiomyocyte hypertrophy has not yet been determined. In this study, we examined whether EPI could inhibit cardiac hypertrophy. METHODS: We utilised cultured neonatal mouse cardiomyocytes and mice for immunofluorescence, immunochemistry, qRT-PCR, and western blot analyses...
2017: Cellular Physiology and Biochemistry
Wenjing Hu, Tongda Xu, Pei Wu, Defeng Pan, Junhong Chen, Jing Chen, Buchun Zhang, Hong Zhu, Dongye Li
We previously found that luteolin (Lut) appeared to improve the contractility of cardiomyocytes during ischemia/reperfusion in rats. The enhancement was associated with the alteration in sarcoplasmic reticulum Ca2+ -ATPase 2a (SERCA2a). This finding prompted us to consider if the mechanism worked in heart failure (HF). We studied the regulation of SERCA2a by Lut in failing cardiomyocytes and intact heart of rats. Improvement of contractility and the mechanisms centered on SERCA2a were studied in isolated cardiomyocytes and intact heart...
January 23, 2017: Scientific Reports
Rui Li, Hai-Hua Geng, Jie Xiao, Xiao-Teng Qin, Fu Wang, Jun-Hui Xing, Yan-Fei Xia, Yang Mao, Jing-Wen Liang, Xiao-Ping Ji
miRs (microRNAs, miRNAs) intricately regulate physiological and pathological processes. Although miR-7a/b protects against cardiomyocyte injury in ischemia/reperfusion injury, the function of miR-7a/b in myocardial infarction (MI)-induced cardiac remodeling remains unclear. Here, we sought to investigate the function of miR-7a/b in post-MI remodeling in a mouse model and to determine the underlying mechanisms involved. miR-7a/b overexpression improved cardiac function, attenuated cardiac remodeling and reduced fibrosis and apoptosis, whereas miR-7a/b silencing caused the opposite effects...
July 7, 2016: Scientific Reports
Hai-Hua Geng, Rui Li, Ya-Min Su, Jie Xiao, Min Pan, Xing-Xing Cai, Xiao-Ping Ji
OBJECTIVES: Recent studies have demonstrated the role of Cdr1as (or CiRS-7), one of the well-identified circular RNAs (circRNAs), as a miR-7a/b sponge or inhibitor in brain tissues or islet cells. This study aimed to investigate the presence of Cdr1as/miR-7a pathway in cardiomyocytes, and explore the mechanism underlying the function of miR-7a in protecting against myocardial infarction (MI)-induced apoptosis. METHODS: Mouse MI injury model was established and evaluated by infarct size determination...
2016: PloS One
Peter Tzu-Yu Chien, Chih-Chung Lin, Li-Der Hsiao, Chuen-Mao Yang
Carbon monoxide (CO) is one of the cytoprotective byproducts of heme oxygenase (HO)-1 and exerts anti-inflammatory action in various models. However, the detailed mechanisms underlying CO-induced HO-1 expression in primary human cardiomyocytes remain largely unidentified. We used primary left ventricle myocytes as a model and applied CO releasing molecule (CORM)-2 to investigate the relationship of CO and HO-1 expression. We herein used Western blot, real-time PCR, promoter activity and EIA to investigate the role of HO-1 expression protecting against thrombin-mediated responses...
December 1, 2015: Toxicology and Applied Pharmacology
Ilimbek Beketaev, Yi Zhang, Kuo-Chan Weng, Siyeon Rhee, Wei Yu, Yu Liu, Jesse Mager, Jun Wang
BACKGROUND: Mesp1 is critical for early cardiomyocyte differentiation and heart development. We previously observed down-regulation of Mesp1 expression in YY1-ablated mouse embryonic hearts. However, how Mesp1 expression is mediated by YY1 is not well understood. RESULTS: We excised YY1 in the murine embryos using Sox2-cre and found that Mesp1 was down-regulated in the embryonic day (E) 7.5 mutant embryos. Also, YY1 activated the 6 kb Mesp1 regulatory element fused to a luciferase reporter...
March 2016: Developmental Dynamics: An Official Publication of the American Association of Anatomists
Qin Li, Peiye Shen, Siyu Zeng, Peiqing Liu
The transforming growth factor (TGF-β)-inducible early gene (TIEG1), a member of the Sp1/Krüppel-like family of transcription factors, plays an important role in regulating cell growth, differentiation, and apoptosis in many human diseases, including breast cancer, osteoporosis, and atherosclerosis. However, little is known about the role of TIEG1 in the heart. In this study, we investigated the role of TIEG1 in angiotensin II (Ang II)-induced cardiomyocyte hypertrophy and its underlying mechanism. Our results showed that TIEG1 expression was downregulated in Ang II-induced hypertrophic cardiomyocytes...
August 2015: Journal of Cardiovascular Pharmacology
Long Wang, Zhi-Ping Tang, Wei Zhao, Bing-Hai Cong, Jian-Qiang Lu, Xiao-Lu Tang, Xiao-Han Li, Xiao-Yan Zhu, Xin Ni
Hydrogen sulfide, generated in the myocardium predominantly via cystathionine-γ-lyase (CSE), is cardioprotective. Our previous study has shown that estrogens enhance CSE expression in myocardium of female rats. The present study aims to explore the mechanisms by which estrogens regulate CSE expression, in particular to clarify the role of estrogen receptor subtypes and the transcriptional factor responsible for the estrogenic effects. We found that either the CSE inhibitor or the CSE small interfering RNA attenuated the protective effect of 17β-estradiol (E2) against H2O2- and hypoxia/reoxygenation-induced injury in primary cultured neonatal cardiomyocytes...
June 2015: Endocrinology
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