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Sagar Paudel, Pankaj Baral, Laxman Ghimire, Scott Bergeron, Liliang Jin, Joseph A De Corte, John T Le, Shanshan Cai, Samithamby Jeyaseelan
Neutrophil migration to the site of bacterial infection is a critical step in host defense. Exclusively produced in the bone marrow, neutrophil release into the blood is tightly controlled. Although the chemokine CXCL1 induces neutrophil influx during bacterial infections, its role in regulating neutrophil recruitment, granulopoiesis, and neutrophil mobilization in response to lung infection-induced sepsis is unclear. Here, we used a murine model of intrapulmonary S. pneumoniae infection to investigate the role of CXCL1 in host defense, granulopoiesis, and neutrophil mobilization...
February 5, 2019: Blood
Zhi-Jie Liang, Xiang Lu, Dan-Dan Zhu, Xiao-Lin Yi, Fang-Xiao Wu, Ning He, Chao Tang, Chang-Yuan Wei, Hong-Mian Li
Augmenting the biological function of adipose-derived stromal cells (ASCs) is a promising approach to promoting tissue remodeling in regenerative medicine. Here, we examined the effect of ginsenoside Rg1 on the paracrine activity and adipogenic differentiation capacity of human breast ASCs (hbASCs) in vitro. hbASCs were isolated and characterized in terms of stromal cell surface markers and multipotency. Third-passage hbASCs were cultured in basic media only or basic media containing different concentrations of G-Rg1 (0...
January 24, 2019: Cell Transplantation
Mayra Ramos de Jesus Pereira, Valéria Rodrigues Pinhatti, Maiele Dornelles da Silveira, Cristina Araujo Matzenbacher, Thales Renato Ochotorena de Freitas, Juliana da Silva, Melissa Camassola, Nance Beyer Nardi
Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil...
November 29, 2018: Genetics and Molecular Biology
Fernanda Marconi Roversi, Nathalia Moreno Cury, Matheus Rodrigues Lopes, Karla Priscila Ferro, João Agostinho Machado-Neto, Marisa Claudia Alvarez, Gabriela Pereira Dos Santos, Renata Giardini Rosa, Ana Leda Longhini, Adriana da Silva Santos Duarte, Fernando Vieira Pericole, Patricia Favaro, José Andres Yunes, Sara Teresinha Olalla Saad
The role of tumour microenvironment in neoplasm initiation and malignant evolution has been increasingly recognized. However, the bone marrow mesenchymal stromal cell (BMMSC) contribution to disease progression remains poorly explored. We previously reported that the expression of serine protease inhibitor kunitz-type2 (SPINT2/HAI-2), an inhibitor of hepatocyte growth factor (HGF) activation, is significantly lower in BMMSC from myelodysplastic syndromes (MDS) patients compared to healthy donors (HD). Thus, to investigate whether this loss of expression was due to SPINT2/HAI-2 methylation, BMMSC from MDS and de novo acute myeloid leukaemia (de novo AML) patients were treated with 5-Azacitidine (Aza), a DNA methyltransferase inhibitor...
November 28, 2018: Journal of Cellular and Molecular Medicine
Mariele Guerra, Tania Luna, Anselmo Souza, Camila Amorim, Natália B Carvalho, Lucas Carvalho, Davi Tanajura, Luciana S Cardoso, Edgar M Carvalho, Silvane Santos
BACKGROUND: HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) is related with high proviral load, high proinflammatory cytokine levels, and passage of infected cell from the blood to the central nervous system. We aimed to evaluate the participation of chemokines and adhesion molecules in HAM/TSP pathogenesis. METHODS: CXCL9, CXCL10, sICAM-1, and sVCAM-1 were determined by ELISA in serum and cerebrospinal fluid (CSF) of HTLV-1 infected individuals...
December 2018: Cellular Immunology
Atousa Khalatbari, Mehdi Mahdavi, Fahimeh Jafarnezhad, Sanaz Afraei, Farzaneh Tofighi Zavareh, Zahra Aghazadeh, Afshin Ghaderi, Abbas Mirshafiey
This investigation evaluates the pro-apoptotic and anti-inflammatory effects of β-D-mannuronic acid [M2000] compared to diclofenac, based on gene expression involved in apoptosis and inflammation process [including Bcl2, NFκB, IL-8 and Cd49d] in peripheral blood mononuclear cells [PBMCs] of healthy donors under exvivo conditions. The venous blood of twelve healthy volunteers with aged 25-60 years, were collected in heparinized tubes. The healthy volunteers were selected from no smoking group and without using illicit drugs and suffering from diabetes...
November 9, 2018: Current Drug Discovery Technologies
Juhyun Oh, Angela Magnuson, Christophe Benoist, Mikael J Pittet, Ralph Weissleder
Cancer incidence increases with age, but paradoxically, cancers have been found to grow more quickly in young mice compared with aged ones. The cause of differential tumor growth has been debated and, over time, attributed to faster tumor cell proliferation, decreased tumor cell apoptosis, and/or increased angiogenesis in young animals. Despite major advances in our understanding of tumor immunity over the past 2 decades, little attention has been paid to comparing immune cell populations in young and aged mice...
November 2, 2018: JCI Insight
Hye Rim Cho, Hyejin Jeon, Chul-Kee Park, Sung-Hye Park, Seung Hong Choi
Although there have been a plethora of radiogenomics studies related to glioblastoma (GBM), most of them only used genomic information from tumor cells. In this study, we used radiogenomics profiling to identify MRI-associated immune cell markers in GBM, which was also correlated with prognosis. Expression levels of immune cell markers were correlated with quantitative MRI parameters in a total of 60 GBM patients. Fourteen immune cell markers (i.e., CD11b, CD68, CSF1R, CD163, CD33, CD123, CD83, CD63, CD49d and CD117 for myeloid cells, and CD4, CD3e, CD25 and CD8 for lymphoid cells) were selected for RNA-level analysis using quantitative RT-PCR...
October 30, 2018: Scientific Reports
Sorour Nemati, Hamed Alizadeh Sardroud, Ali Baradar Khoshfetrat, Majid Khaksar, Mahdi Ahmadi, Hassan Amini, Shirin Saberianpour, Aref Delkhosh, Ali Akbar Movassaghpour, Reza Rahbarghazi
Today, many attempts have been collected in the field of tissue engineering for reconstitution of injured bone marrow capacity by transplantation of functional cell source. By having a 3D condition, microcapsules are appropriate candidates for cells transplantation to target sites. Here, we examined the effect of alginate-gelatin microcapsules on functional maturation of human myelomonocytic cell line U937 after 7 days in vitro. U937 cells were encapsulated by the mixture of alginate-gelatin and cultured for 7 days...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, Valter Gattei
The integrin heterodimer CD49d/CD29 (a.k.a. Very Late Antigen-4, VLA-4) mediates cell-cell and cell-matrix interaction through the binding of its ligands VCAM-1 and fibronectin. VLA-4 can be present on the cell surface at different conformation states that affect the binding affinity for the ligands. In chronic lymphocytic leukemia (CLL), higher VLA-4 levels, as determined by measuring the expression of CD49d chain by flow cytometry, have been demonstrated to associate with a worse prognosis, in keeping with the role of VLA-4 as key molecule favoring CLL cell localization in protective niches of bone marrow and lymph nodes...
2019: Methods in Molecular Biology
Nadia Naseer, Saliha Bashir, Noreen Latief, Farzana Latif, Shaheen N Khan, Sheikh Riazuddin
AIM: The aim of the present study is to use human amniotic membrane (HAM) for in vitro chondrogenesis of placenta-derived mesenchymal stem cells (MSCs) and umbilical cord-derived MSCs. MATERIALS & METHODS: MSCs from the placenta and umbilical cord were isolated, characterized by immunophenotyping and after analyzing their rate of proliferation, cytotoxicity and viability, chondrogenesis was performed on plastic adherent surface and on HAM. RESULTS: Successfully isolated and characterized placenta-derived MSCs and umbilical cord-derived MSCs revealed positive expression of MSCs markers CD90, CD73, CD105 and CD49d, while they were negative for CD45...
October 2018: Regenerative Medicine
F Rezaei, T Tiraihi, A Abdanipour, H K Hassoun, T Taheri
Valproic acid (VPA) is an inhibitor of histone deacetylases (HDACs) that can regulate differentiation and proliferation of stem cells by epigenetic mechanisms. We investigated VPA induced histone H3 and H4 acetylation in adipose derived stem cells (ADSCs) transdifferentiated into neuron-like cells (NLCs). Rat ADSCs were transdifferentiated into neural stem cells (NSCs) that had been generated from neurospheres. The NSCs were differentiated into NLCs by induction with different concentrations of VPA at 24, 48 and 72 h...
October 1, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
Neus Giménez, Alejandra Martínez-Trillos, Arnau Montraveta, Mónica Lopez-Guerra, Laia Rosich, Ferran Nadeu, Juan G Valero, Marta Aymerich, Laura Magnano, Maria Rozman, Estella Matutes, Julio Delgado, Tycho Bauman, Eva Gine, Marcos González, Miguel Alcoceba, M Jose Terol, Blanca Navarro, Enrique Colado, Angel R Payer, Xose S Puente, Carlos López-Otín, Armando Lopez-Guillermo, Elias Campo, Dolors Colomer, Neus Villamor
Mutations in genes of the RAS-BRAF-MAPK-ERK pathway have not been fully explored in chronic lymphocytic leukemia patients. To provide a better comprehension, we analyzed the clinical and biological characteristics of patients with mutations in this pathway and investigated the in vitro response of primary cells to BRAF and ERK inhibitors. Putative damaging mutations were found in 25 of 452 patients (5.5%). Of those, BRAF was mutated in 9 patients (2.0%), genes upstream of BRAF (KITLG, KIT, PTPN11, GNB1, KRAS and NRAS) were mutated in 12 patients (2...
September 27, 2018: Haematologica
XiaoSan Su, Liu Yang, YanFeng Yin, Jie Huang, Fei Qiao, Yu Fang, Lu Yu, YinYin Wang, KaiHua Zhou, Jun Wang
BACKGROUND: Bone marrow mesenchymal stem cells (BMSC) transfer has been attempted as a therapeutic strategy in experimental lung injury and fibrosis. Reduction of neutrophilic infiltration is one of the mechanisms involved in this effect. However, the mechanisms by which BMSC modulate neutrophil remains unknown. METHODS AND RESULTS: Exposure of mice to bleomycin (BLM) resulted in significant accumulation of cells that express neutrophilic markers Gr-1High CD11b+ Ly-6GHigh F4/80- CD115- CD49d- ...
September 26, 2018: Stem Cell Research & Therapy
Oskar McWilliam, Finn Sellebjerg, Hanne V Marquart, Marina Rode von Essen
The contribution of B cells to the pathogenesis of relapsing-remitting multiple sclerosis (RRMS) is currently of great interest due to the positive outcomes of treatment with B cell-depleting monoclonal antibodies. In this exploratory study we examined the phenotype and cytokine response of B cells from untreated patients with RRMS and healthy controls. The CNS migration potential of the individual blood B cell subpopulations was evaluated according to the expression of CD49d, ALCAM, CXCR3, and CCR7, and cerebrospinal fluid (CSF) samples were analyzed to establish the phenotype of migrated B cells...
November 15, 2018: Journal of Neuroimmunology
Dimitrios Chanouzas, Michael Sagmeister, Lovesh Dyall, Phoebe Sharp, Lucy Powley, Serena Johal, Jessica Bowen, Peter Nightingale, Charles J Ferro, Matthew D Morgan, Paul Moss, Lorraine Harper
BACKGROUND: Cardiovascular disease is a leading cause of death in ANCA-associated vasculitis (AAV). An expansion of CD4+ CD28null T cells is seen mainly in cytomegalovirus (CMV)-seropositive individuals and has been linked to increased cardiovascular disease risk in other conditions. The aims of this study were to phenotype CD4+ CD28null T cells in AAV with respect to their pro-inflammatory capacity and ability to target and damage the endothelium and to investigate their relationship to arterial stiffness, a marker of cardiovascular mortality...
August 29, 2018: Arthritis Research & Therapy
Lisa Borkner, Alicja Misiak, Mieszko M Wilk, Kingston H G Mills
Treatment with the macrolide antibiotic azithromycin (AZM) is an important intervention for controlling infection of children with Bordetella pertussis and as a prophylaxis for preventing transmission to family members. However, antibiotics are known to have immunomodulatory effects independent of their antimicrobial activity. Here, we used a mouse model to examine the effects of AZM treatment on clearance of B. pertussis and induction of innate and adaptive immunity. We found that treatment of mice with AZM either 7 or 14 days post challenge effectively cleared the bacteria from the lungs...
2018: Frontiers in Immunology
Jae-Ghi Lee, Joon Young Jang, Taishi Fang, Yixuan Xu, Ji-Jing Yan, Jung-Hwa Ryu, Hee Jung Jeon, Tai Yeon Koo, Dong Ki Kim, Kook-Hwan Oh, Tae Jin Kim, Jaeseok Yang
Human B-1 cells have been proposed to be CD20+ CD27+ CD43+ CD1c- B cells found in the umbilical cord and adult peripheral blood, but their regulatory mechanisms have not been well elucidated. Previously, we reported that mouse CD49dhigh CD4+ T cells could enhance the secretion of natural antibodies by B-1 cells. In this study, we aimed to investigate the presence and helper functions of the human equivalents of murine CD49dhigh CD4+ T cells. Here, we showed that human CD49dhigh CD4+ T cells found in the peritoneal cavity (PEC), spleen, and peripheral blood can enhance the production of IgM antibodies by B-1 cells...
2018: Frontiers in Immunology
Lisa Lohmann, Claudia Janoschka, Andreas Schulte-Mecklenbeck, Svenja Klinsing, Lucienne Kirstein, Uta Hanning, Timo Wirth, Tilman Schneider-Hohendorf, Nicholas Schwab, Catharina C Gross, Maria Eveslage, Sven G Meuth, Heinz Wiendl, Luisa Klotz
Leukocyte sequestration is an established therapeutic concept in multiple sclerosis (MS) as represented by the trafficking drugs natalizumab (NAT) and fingolimod (FTY). However, the precise consequences of targeting immune cell trafficking for immunoregulatory network functions are only incompletely understood. In the present study, we performed an in-depth longitudinal characterization of functional and phenotypic immune signatures in peripheral blood (PB) and cerebrospinal fluid (CSF) of 15 MS patients during switching from long-term NAT to FTY treatment after a defined 8-week washout period within a clinical trial (ToFingo successor study; ClinicalTrials...
2018: Frontiers in Immunology
Richard Thomson-Luque, Chengqi Wang, Francis B Ntumngia, Shulin Xu, Karoly Szekeres, Amy Conway, Swamy Rakesh Adapa, Samantha J Barnes, John H Adams, Rays H Y Jiang
Progress towards an in-depth understanding of the final steps of the erythroid lineage development is paramount for many hematological diseases. We have characterized the final stages of reticulocyte maturation from bone marrow to peripheral blood using for the first time single-cell Mass Cytometry (CyTOF). We were able to measure the expression of 31 surface markers within a single red blood cell (RBC). We demonstrate the validity of CyTOF for RBC phenotyping by confirming the progressive reduction of transferrin receptor 1 (CD71) during reticulocyte maturation to mature RBC...
September 2018: Blood Cells, Molecules & Diseases
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