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Daniel B Russakoff, Ali Lamin, Jonathan D Oakley, Adam M Dubis, Sobha Sivaprasad
Purpose: To develop and assess a method for predicting the likelihood of converting from early/intermediate to advanced wet age-related macular degeneration (AMD) using optical coherence tomography (OCT) imaging and methods of deep learning. Methods: Seventy-one eyes of 71 patients with confirmed early/intermediate AMD with contralateral wet AMD were imaged with OCT three times over 2 years (baseline, year 1, year 2). These eyes were divided into two groups: eyes that had not converted to wet AMD (n = 40) at year 2 and those that had (n = 31)...
February 1, 2019: Investigative Ophthalmology & Visual Science
Jinyang Chen, Xiaodong Dong, Qing Zhang, Shumin Ding
PET/TiO2 composite film is the most widely used film for polymer-laminated steel, and the migration of TiO2 is very important for the safety evaluation of its packaged food. Microwave digestion, wet digestion and dry ashing were used for pretreatment of composite film to determine the content of TiO2 in composite film. Migration tests were carried out at 40°C, 60°C and 80°C 4% using acetic acid and 50% ethanol as the acid and ester food simulants. The migration amount of TiO2 was determined by inductively coupled plasma optical emission spectrometry (ICP-OES)...
February 20, 2019: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Grenalynn C Ilacas, Alexis Basa, Katherine J Nelms, Joshua D Sosa, Yangyang Liu, Frank A Gomez
This paper describes the development of two microfluidic paper-based analytical devices (μPADs), one well-based and the other based on a lateral flow assay (LFA) configuration, to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Zr-PCN-222(Fe), to encapsulate glucose oxidase (GOx). The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx@MOF species sandwiched between the paper layers realizing a yellow-brown color...
May 9, 2019: Analytica Chimica Acta
Salim Lamine, George P Petropoulos, Paul A Brewer, Nour-El-Islam Bachari, Prashant K Srivastava, Kiril Manevski, Chariton Kalaitzidis, Mark G Macklin
Technological advances in hyperspectral remote sensing have been widely applied in heavy metal soil contamination studies, as they are able to provide assessments in a rapid and cost-effective way. The present work investigates the potential role of combining field and laboratory spectroradiometry with geochemical data of lead (Pb), zinc (Zn), copper (Cu) and cadmium (Cd) in quantifying and modelling heavy metal soil contamination (HMSC) for a floodplain site located in Wales, United Kingdom. The study objectives were to: (i) collect field- and lab-based spectra from contaminated soils by using ASD FieldSpec® 3, where the spectrum varies between 350 and 2500 nm; (ii) build field- and lab-based spectral libraries; (iii) conduct geochemical analyses of Pb, Zn, Cu and Cd using atomic absorption spectrometer; (iv) identify the specific spectral regions associated to the modelling of HMSC; and (v) develop and validate heavy metal prediction models (HMPM) for the aforementioned contaminants, by considering their spectral features and concentrations in the soil...
February 13, 2019: Sensors
Iván Méndez-López, Idoia Blanco-Luquin, Javier Sánchez-Ruiz de Gordoa, Amaya Urdánoz-Casado, Miren Roldán, Blanca Acha, Carmen Echavarri, Victoria Zelaya, Ivonne Jericó, Maite Mendioroz
Lamins are fibrillary proteins that are crucial in maintaining nuclear shape and function. Recently, B-type lamin dysfunction has been linked to tauopathies. However, the role of A-type lamin in neurodegeneration is still obscure. Here, we examined A-type and B-type lamin expression levels by RT-qPCR in Alzheimer's disease (AD) patients and controls in the hippocampus, the core of tau pathology in the brain. LMNA , LMNB1, and LMNB2 genes showed moderate mRNA levels in the human hippocampus with highest expression for the LMNA gene...
February 18, 2019: International Journal of Molecular Sciences
Marianne Grafe, Petros Batsios, Irene Meyer, Daria Lisin, Otto Baumann, Martin W Goldberg, Ralph Gräf
Nuclear lamins are nucleus-specific intermediate filaments (IF) found at the inner nuclear membrane (INM) of the nuclear envelope (NE). Together with nuclear envelope transmembrane proteins, they form the nuclear lamina and are crucial for gene regulation and mechanical robustness of the nucleus and the whole cell. Recently, we characterized Dictyostelium NE81 as an evolutionarily conserved lamin-like protein, both on the sequence and functional level. Here, we show on the structural level that the Dictyostelium NE81 is also capable of assembling into filaments, just as metazoan lamin filament assemblies...
February 16, 2019: Cells
Francesca Chiarini, Camilla Evangelisti, Vittoria Cenni, Antonietta Fazio, Francesca Paganelli, Alberto M Martelli, Giovanna Lattanzi
The mechanistic target of rapamycin (mTOR) is a ubiquitous serine/threonine kinase that regulates anabolic and catabolic processes, in response to environmental inputs. The existence of mTOR in numerous cell compartments explains its specific ability to sense stress, execute growth signals, and regulate autophagy. mTOR signaling deregulation is closely related to aging and age-related disorders, among which progeroid laminopathies represent genetically characterized clinical entities with well-defined phenotypes...
February 15, 2019: International Journal of Molecular Sciences
Zhuxiu Qian, Yiwen Xu, Chaofan Zheng, Anping Zhang, Jianqiang Sun
The emissions of brominated flame retardants (BFRs) from consumer products have been considered the major to the ubiquitous occurrence of contaminants in indoor environments. Direct contact with dust covering the surface of source materials in a real environment could introduce significant uncertainty. This study investigated the effects of dust coverage on the emissions of four BFRs, including 1, 2, 5, 6, 9, and 10-hexabromocyclododecane (HBCD), bis(2-ethyl-1-hexyl) tetrabromophthalate (BEHTBP), tetrabromobisphenol A (TBBPA), and hexabromobenzene (HBBZ), from decorative laminate, cotton sound insulation, PVC floor, and carpet...
February 19, 2019: Environmental Monitoring and Assessment
Ergin Beyret, Hsin-Kai Liao, Mako Yamamoto, Reyna Hernandez-Benitez, Yunpeng Fu, Galina Erikson, Pradeep Reddy, Juan Carlos Izpisua Belmonte
Hutchinson-Gilford progeria syndrome (HGPS) is a rare lethal genetic disorder characterized by symptoms reminiscent of accelerated aging. The major underlying genetic cause is a substitution mutation in the gene coding for lamin A, causing the production of a toxic isoform called progerin. Here we show that reduction of lamin A/progerin by a single-dose systemic administration of adeno-associated virus-delivered CRISPR-Cas9 components suppresses HGPS in a mouse model.
February 18, 2019: Nature Medicine
Giangiacomo Minak, Tommaso M Brugo, Cristiano Fragassa, Ana Pavlovic, Felipe V de Camargo, Nicola Zavatta
Cruisers are multi-occupant solar vehicles that are conceived to compete in long-range (over 3,000 km) solar races based on the best compromise between the energy consumption and the payload. They must comply to the race's rules regarding the overall dimensions, the solar panel size, functionality, and safety and structural requirements, while the shape, the materials, the powertrain, and the mechanics are considered at the discretion of the designer. In this work, the most relevant aspects of the structural design process of a full-carbon fiber-reinforced plastic solar vehicle are detailed...
January 30, 2019: Journal of Visualized Experiments: JoVE
S M Wang, Z Li, G B Wang, H Q Ye, Y S Liu, D Tong, W H Gao, Y S Zhou
OBJECTIVE: To establish a complete workflow of digital design and manufacturing occlusal splint for sleep bruxism, which can be preliminarily applied in clinical use, thus observe the clinical efficacy. METHODS: Twenty-four patients with sleep bruxism were recruited in the study and randomly divided into two groups by using random number tables. Digital-occlusal-splint (experimental group) treatment plan and traditional-occlusal-splint (control-group) treatment plan were carried out for each group, respectively...
February 18, 2019: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
Y J Ge, X Q Liu
OBJECTIVE: To assess and compare the effects of loupes and microscope on laminate veneer preparation of the first practitioner from the aspects of efficiency, quality and accuracy of preparation, and preference. METHODS: Twenty young prosthodontists from the Department of Prosthodontics, Peking University School and Hospital of Stomatology were recruited into this study, which was prospective, single blind, self-control trials. The participants had no experience of using dental magnification devices...
February 18, 2019: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
C Michael Honey, Zurab Ivanishvili, Christopher R Honey, Manraj K S Heran
OBJECTIVEThe location of the human spinothalamic tract (STT) in the anterolateral spinal cord has been known for more than a century. The exact nature of the neuronal fiber lamination within the STT, however, remains controversial. After correlating in vivo macrostimulation-induced pain/temperature sensation during percutaneous cervical cordotomy with simultaneous CT imaging of the electrode tip location, the authors present a modern description of the somatotopy of the human cervical STT.METHODSTwenty patients underwent CT-guided percutaneous cervical cordotomy to alleviate contralateral medication-refractory cancer pain...
February 15, 2019: Journal of Neurosurgery. Spine
Alessia Prato, Marco L Longana, Ambreen Hussain, Michael R Wisnom
This work experimentally explores the post-impact behaviour of thin-ply angle-ply pseudo-ductile carbon fibre laminates subjected to tensile load. Indentation and low-speed impact tests were performed on standard tensile test specimens. Non-destructive tests were used to investigate the damage propagation. Digital Image Correlation (DIC) was adopted to detect the strain distribution during tensile tests. Post-damage pseudo-ductile behaviour was retained in angle-ply hybrid composites subjected to tensile loading conditions...
February 15, 2019: Materials
Jianfeng Dai, Shangbin Xi, Dongna Li
A multi-physics coupling numerical model of the curing process is proposed for the thermosetting resin composites in this paper, and the modified "cure hardening instantaneously linear elastic (CHILE)" model and viscoelastic model are adopted to forecast residual stress and deformation during the curing process. The thermophysical properties of both models are evolved in line with temperature and degree of cure (DOC). Accordingly, the numerical simulation results are improved to be more accurate. Additionally, the elastic modulus of the materials is calibrated to be equal to the modulus of viscoelastic relaxation by a defined function of time in the CHILE model...
February 14, 2019: Materials
Md Imamul Islam, Pandian Nagakannan, Olamide Ogungbola, Jelena Djordjevic, Benedict C Albensi, Eftekhar Eftekharpour
Recent reports in pathophysiology of neurodegenerative diseases (ND) have linked nuclear lamina degradation/deficits to neuronal cell death. Lamin-B1 damage is specifically involved in this process leading to nuclear envelope invagination and heterochromatin rearrangement. The underlying mechanisms involved in these events are not yet defined. In this study, while examining the effect of Thioredoxin-1(Trx1) inhibition on cell death in a model of oxidative stress, we noted robust nuclear invagination in SH-SY5Y cells...
February 12, 2019: Free Radical Biology & Medicine
Elisabetta Mattioli, Davide Andrenacci, Antonello Mai, Sergio Valente, Joke Robijns, Winnok H De Vos, Cristina Capanni, Giovanna Lattanzi
We recently identified lamin A/C as a docking molecule for human histone deacetylase 2 (HDAC2) and showed involvement of HDAC2-lamin A/C complexes in the DNA damage response. We further showed that lamin A/C-HDAC2 interaction is altered in Hutchinson-Gilford Progeria syndrome and other progeroid laminopathies. Here, we show that both inhibitors of lamin A maturation and small molecules inhibiting HDAC activity affect lamin A/C interaction with HDAC2. While statins, which inhibit prelamin A processing, reduce protein interaction, HDAC inhibitors strengthen protein binding...
2019: Frontiers in Cell and Developmental Biology
Yong-Jie Zhang, Lin Guo, Patrick K Gonzales, Tania F Gendron, Yanwei Wu, Karen Jansen-West, Aliesha D O'Raw, Sarah R Pickles, Mercedes Prudencio, Yari Carlomagno, Mariam A Gachechiladze, Connor Ludwig, Ruilin Tian, Jeannie Chew, Michael DeTure, Wen-Lang Lin, Jimei Tong, Lillian M Daughrity, Mei Yue, Yuping Song, Jonathan W Andersen, Monica Castanedes-Casey, Aishe Kurti, Abhishek Datta, Giovanna Antognetti, Alexander McCampbell, Rosa Rademakers, Björn Oskarsson, Dennis W Dickson, Martin Kampmann, Michael E Ward, John D Fryer, Christopher D Link, James Shorter, Leonard Petrucelli
How hexanucleotide GGGGCC (G4 C2 ) repeat expansions in C9orf72 cause frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) is not understood. We developed a mouse model engineered to express poly(PR), a proline-arginine (PR) dipeptide repeat protein synthesized from expanded G4 C2 repeats. The expression of green fluorescent protein-conjugated (PR)50 (a 50-repeat PR protein) throughout the mouse brain yielded progressive brain atrophy, neuron loss, loss of poly(PR)-positive cells, and gliosis, culminating in motor and memory impairments...
February 15, 2019: Science
Bruce Nmezi, Jianquan Xu, Rao Fu, Travis J Armiger, Guillermo Rodriguez-Bey, Juliana S Powell, Hongqiang Ma, Mara Sullivan, Yiping Tu, Natalie Y Chen, Stephen G Young, Donna B Stolz, Kris Noel Dahl, Yang Liu, Quasar S Padiath
The nuclear lamina is an intermediate filament meshwork adjacent to the inner nuclear membrane (INM) that plays a critical role in maintaining nuclear shape and regulating gene expression through chromatin interactions. Studies have demonstrated that A- and B-type lamins, the filamentous proteins that make up the nuclear lamina, form independent but interacting networks. However, whether these lamin subtypes exhibit a distinct spatial organization or whether their organization has any functional consequences is unknown...
February 14, 2019: Proceedings of the National Academy of Sciences of the United States of America
Anna Kurpińska, Joanna Suraj, Emilia Bonar, Agnieszka Zakrzewska, Marta Stojak, Magdalena Sternak, Agnieszka Jasztal, Maria Walczak
INTRODUCTION: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4 T1 mammary adenocarcinoma. MATERIALS AND METHODS: The studies were performed on 7-8-week-old BALB/c female mice...
February 11, 2019: Experimental and Molecular Pathology
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