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endothelial to mesenchymal

Maha Abdelmonem, Nancy N Shahin, Laila A Rashed, Hebat Allah A Amin, Ashraf A Shamaa, Amira A Shaheen
Stem cell therapy represents a promising therapeutic avenue for cardiac disorders, including heart failure. Although stem cell transplantation showed encouraging preliminary results, the outcomes of clinical studies are still unsatisfactory. This study aimed to compare the outcomes of two therapeutic approaches, in vivo co-delivery of sodium hydrogen sulfide (NaHS) concomitant with bone marrow-derived mesenchymal stem cell (BMSC) transplantation and in vitro preconditioning of BMSCs with NaHS, both of which are intended to promote the success of stem cell therapy in rats with isoprenaline-induced heart failure...
February 18, 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
I B Sokolova, G I Lobov
Intracerebral transplantation of mesenchymal stem cells to 6- and 12-month-old SHR rats induced angiogenesis in the pia mater. In 6-months-old SHR rats, perfusion in the brain tissue after cell transplantation considerably increased, while in 12-month-old rats it remained practically unchanged. We also observed marked activation of regulatory processes in the cerebral vascular system, most pronounced in 12-month-old rats. Neurogenic and myogenic tone of cerebral vessels increased significantly, while endothelium-dependent tone slightly decreased...
February 19, 2019: Bulletin of Experimental Biology and Medicine
Mohamed H Abumaree, Seham Al Harthy, Abdullah M Al Subayyil, Manal A Alshabibi, Fawaz M Abomaray, Tanvier Khatlani, Bill Kalionis, Mohammed F El-Muzaini, Mohammed A Al Jumah, Dunia Jawdat, Abdullah O Alawad, Ahmed S AlAskar
Placental mesenchymal stem cells from maternal decidua basalis tissue (DBMSCs) are promising cells for tissue repair because of their multilineage differentiation and ability to protect endothelial cells from injury. Here, we examined DBMSC interaction with macrophages and whether this interaction could modulate the characteristics and functions of these macrophages. We induced monocytes to differentiate into M1-like macrophages in the presence of DBMSCs. DBMSC effects on differentiation were evaluated using microscopy, flow cytometry, and ELISA...
February 18, 2019: Cells
Qiuchen Qi, Yanhong Hou, Ang Li, Yueyue Sun, Siying Li, Zhongxi Zhao
This study was aimed to investigate the anti-tumor, anti-metastasis and immunomodulatory effects of Yifei Tongluo (YFTL), a Chinese herbal formula, in Lewis lung carcinoma mice and to explore the underlying mechanisms. LLC cells were inoculated subcutaneously in C57BL/6 mice to establish the Lewis lung carcinoma model. We observed that YFTL effectively inhibited tumor growth and prolonged the overall survival of tumor-bearing mice. Additionally, YFTL treatment resulted in a significantly decreased number of surface lung metastatic lesions compared with the model control group...
February 18, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Saba Aslani, Mahboubeh Kabiri, Mousa Kehtari, Hana Hanaee-Ahvaz
As the incidence of small-diameter vascular graft (SDVG) occlusion is considerably high, a great amount of research is focused on constructing a more biocompatible graft. The absence of a biocompatible surface in the lumen of the engineered grafts that can support confluent lining with endothelial cells (ECs) can cause thrombosis and graft failure. Blood clot formation is mainly because of the lack of an integrated endothelium. The most effective approach to combat this problem would be using natural extracellular matrix constituents as a mimic of endothelial basement membrane along with applying anticoagulant agents to provide local antithrombotic effects...
February 18, 2019: Journal of Cellular Physiology
Bo Ye, Liwen Li, Haodong Xu, Yiping Chen, Faqian Li
Bone morphogenetic protein (BMP) and Wnt pathways regulate cell proliferation and differentiation, but how these two pathways interact and mediate their nuclear actions in the heart, especially during late cardiac development, remains poorly defined. T-cell factor (TCF) and lymphoid enhancer factor (LEF) family transcriptional factors, including Lef1, Tcf7, Tcf7l1, and Tcf7l2, are important nuclear mediators of canonical Wnt/β-catenin signaling throughout cardiac development. We reveal that these TCF/LEF family members direct heart maturation through distinct temporal and spatial control...
February 18, 2019: Laboratory Investigation; a Journal of Technical Methods and Pathology
Pulavendran Sivasami, Nabin Poudel, Maria Cristina Munteanu, Joanna Hudson, Pamela Lovern, Lin Liu, Tim Griffin, Myron E Hinsdale
BACKGROUND/OBJECTIVES: The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood. Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity. Therefore, PGs and their GAGs could be significant to adipose tissue homeostasis. The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis...
February 18, 2019: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Xiao Guo, Shenghai Lee, Peilong Cao
Background: Breast cancer (BC) has been the commonest malignant tumor with a low survival rate among woman. Long non-coding RNA hypoxia-inducible factor-1 alpha antisense RNA-2 (HIF1A-AS2) was correlated with various cancers. Purpose: The study aimed to investigate the roles and related underlying molecular mechanisms of HIF1A-AS2 in BC. Material and methods: Target relationships were speculated by Targetscan 7.0 and confirmed by dual luciferase reporter assay...
2019: OncoTargets and Therapy
Yang Liu, Eleni Dakou, Ying Meng, Luc Leyns
Isolated mouse embryonic stem cells (mESCs) retain the capacities to self-renew limitlessly and to give rise to all tissues of an adult mouse. A precise understanding of the relationships, mechanisms of action and functions of novel genes involved in mESCs differentiation is crucial to expand our knowledge of vertebrate development. The epithelial membrane protein 2 (EMP2) is a membrane-spanning protein found in epithelial and endothelial cell-cell junctions that has been implicated in the regulation of cell proliferation and migration in normal and tumor tissues...
February 14, 2019: Biochemical and Biophysical Research Communications
Ainara Benavente-Babace, Kristina Haase, Duncan J Stewart, Michel Godin
Endothelial progenitor cells (EPCs) and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focused on cell-encapsulation, to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules...
February 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
Charlotte Piard, Hannah Baker, Timur Kamalitdinov, John P Fisher
Bone tissue engineers are facing a daunting challenge when attempting to fabricate bigger constructs intended for use in the treatment of large bone defects, which is the vascularization of the graft. Cell-based approaches and, in particular, the use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) has been one of the most explored options. We present in this paper an alternative method to mimic the spatial pattern of HUVECs and hMSCs found in native osteons based on the use of extrusion-based 3D bioprinting (3DP)...
February 15, 2019: Biofabrication
Kenneth Kaushansky, Huichun Zhan
The hematopoietic niche is composed of endothelial cells, mesenchymal stromal cells of several types, and megakaryocytes, and functions to support the survival, proliferation, and differentiation of normal hematopoietic stem cells (HSCs). An abundance of evidence from a range of hematological malignancies supports the concept that the niche also participates in the pathogenesis of malignant hematopoiesis, differentially supporting malignant stem or progenitor cells over that of normal blood cell development...
February 14, 2019: Annals of the New York Academy of Sciences
Ramla Sana Khalid, Irfan Khan, Midhat Batool Zaidi, Nadia Naeem, Kanwal Haneef, Rida-E-Maria Qazi, Rakhshinda Habib, Tuba Shakil Malick, Anwar Ali, Asmat Salim
This study was aimed to enhance the healing potential of rat bone marrow mesenchymal stem cells against chronic diabetic wounds through interleukin-7 (IL-7) transfection. IL-7 plays an important role in wound healing and acts as a survival factor in some cell types. This study involves isolation, propagation and characterization of MSCs and their modification with IL-7 gene via retroviral transfection. Transfected MSCs were assessed for their effect on angiogenic genes by qPCR. Wound healing potential of transfected MSCs was analyzed by scratch assay in vitro and by transplanting these cells in rat diabetic wound models in vivo...
February 13, 2019: Wound Repair and Regeneration
Irit Meivar-Levy, Fatima Zoabi, Gil Nardini, Eugenia Manevitz-Mendelson, Gil S Leichner, Oranit Zadok, Michael Gurevich, Eytan Mor, Simona Dima, Irinel Popescu, Aviv Barzilai, Sarah Ferber, Shoshana Greenberger
BACKGROUND: Insulin-dependent diabetes is a multifactorial disorder that could be theoretically cured by functional pancreatic islets and insulin-producing cell (IPC) implantation. Regenerative medicine approaches include the potential for growing tissues and organs in the laboratory and transplanting them when the body cannot heal itself. However, several obstacles remain to be overcome in order to bring regenerative medicine approach for diabetes closer to its clinical implementation; the cells generated in vitro are typically of heterogenic and immature nature and the site of implantation should be readily vascularized for the implanted cells to survive in vivo...
February 13, 2019: Stem Cell Research & Therapy
Christa F Gaskill, Jonathan A Kropski, Bradley W Richmond, Robert F Foronjy, M Mark Taketo, Susan M Majka
RATIONALE: Chronic obstructive pulmonary disease (COPD) is the third leading cause of mortality in the United States. COPD and associated emphysematous loss of tissue structure often is exacerbated by vasculopathy, which substantially worsens prognosis and limits survival. Vasculopathy is characterized by remodeling and loss of microvessels. Evidence has also highlighted a role for the alterations to the microvasculature during the early pathogenesis and heterogeneity of disease, although the underlying mechanisms are not defined...
December 2018: Annals of the American Thoracic Society
Ayda Pouyafar, Aysa Rezabakhsh, Reza Rahbarghazi, Milad Zadi Heydarabad, Elhameh Shokrollahi, Emel Sokullu, Majid Khaksar, Alireza Nourazarian, Çığır Biray Avci
In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in endothelial induction medium containing sulindac and resveratrol. Expression of endothelial markers such as the von Willebrand factor (vWF) and vascular endothelial cadherin (VE-cadherin) and genes participating in mesenchymal-to-epithelial transition was studied by real-time PCR assay...
February 13, 2019: Cell and Tissue Research
Olivia Kérourédan, Jean-Michel Bourget, Murielle Rémy, Sylvie Crauste-Manciet, Jérôme Kalisky, Sylvain Catros, Noëlie B Thébaud, Raphaël Devillard
Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells...
February 12, 2019: Journal of Materials Science. Materials in Medicine
Amita Ajit, Santhoshkumar Thankayyan Retnabai, Lissy K Krishnan
Biotechnological interventions to engineer favorable biochemical environments that facilitate wound repair through quick attainment of vasculature are of immense demand. Potential of angiogenic factors (AF), notably vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (Hif-1α) and the paracrine role of human adipose derived mesenchymal stromal cells (hADMSCs) in establishing vasculature has been reported. Independent application of AF or hADMSCs has led to clinical failure, pertaining to poor means of delivery, deprived bioavailability and insufficiency to drive adequate angiogenesis, respectively...
February 12, 2019: Tissue Engineering. Part C, Methods
Sandra Pinho, Paul S Frenette
The haematopoietic stem cell (HSC) microenvironment in the bone marrow, termed the niche, ensures haematopoietic homeostasis by controlling the proliferation, self-renewal, differentiation and migration of HSCs and progenitor cells at steady state and in response to emergencies and injury. Improved methods for HSC isolation, driven by advances in single-cell and molecular technologies, have led to a better understanding of their behaviour, heterogeneity and lineage fate and of the niche cells and signals that regulate their function...
February 11, 2019: Nature Reviews. Molecular Cell Biology
Lewis S C Ward, Lozan Sheriff, Jennifer L Marshall, Julia E Manning, Alexander Brill, Gerard B Nash, Helen M McGettrick
Mesenchymal stromal cells (MSC) up-regulate podoplanin at sites of infection, chronic inflammation, and cancer. We investigated the functional consequences of podoplanin expression on the migratory potential of MSC and their interactions with circulating platelets. Expression of podoplanin significantly enhanced the migration of MSC compared to MSC lacking podoplanin. Rac-1 inhibition altered the membrane localisation of podoplanin and in turn significantly reduced MSC migration. Blocking Rac-1 activity had no effect on the migration of MSC lacking podoplanin, indicating it was responsible for regulation of migration through podoplanin...
February 11, 2019: Journal of Cell Science
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