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confocal and microscopy

Cheng Jiayi, Ning Tianyi, Teng Dan, Kang Tingguo, Wang Qingfeng, Zhang Qianqian
To explore the protective effect and mechanism of isorhamnetin against oxidative injury caused by H2 O2 to endothelial cell strain CRL1730 of human umbilical vein. H2 O2 and endothelial cell strain CRL1730 were used, as a model of injured endothelial cell. Three levels of crude drugs areorhamnetin, 22.8μg/ml, 11.4μg/mL and 5.7μg/mL was added to the injured cell strain CRL1730 respectively. The cell injury was measured in terms of necrotic rate, quantities of von Wilebr and factor (vWf) and thrombomodulin (TM), lactate dehydrogenase (LDH) and intracellular free calcium ions through flow cytometry, ELISA, fluorescent spectrometer and laser scanning confocal microscopy respectively...
January 2019: Pakistan Journal of Pharmaceutical Sciences
Francis Perton, Sébastien Harlepp, Gautier Follain, Ksenia Parkhomenko, Jacky G Goetz, Sylvie Bégin-Colin, Damien Mertz
The engineering of luminescent nanoplatforms for biomedical applications displaying ability for scaling-up, good colloidal stability in aqueous solutions, biocompatibility, and providing an easy detection in vivo by fluorescence methods while offering high potential of functionalities, is currently a challenge. The original strategy proposed here involves the use of large pore (ca. 15 nm) mesoporous silica (MS) nanoparticles (NPs) having a stellate morphology (denoted STMS) on which fluorescent InP/ZnS quantum dots (QDs) are covalently grafted with a high yield (≥90%)...
January 23, 2019: Journal of Colloid and Interface Science
Jianying Huang, Yuhong Liu, Lijun Yang, Fengyan Zhou
A sulfonated chitosan (SCS) was prepared via nucleophilic substitution and characterized by Fourier transform infrared spectroscopy, 1 H and 13 C NMR spectra, gel permeation chromatography, elemental analysis, and thermo gravimetric analysis. The inhibition activities of bacterial adhesion and biofilm formation against E. coli and S. aureus of SCS were assessed in comparison with those of unmodified chitosan hydrochloride (WCS) which was commercially available. The metabolic activity and secretion of exopolysaccharide in biofilms of E...
February 14, 2019: International Journal of Biological Macromolecules
Xiong-Jie Zhao, Yu-Ting Li, Yu-Ren Jiang, Bing-Qing Yang, Ce Liu, Zhi-Hong Liu
Hydrogen sulfide (H2 S) has been considered to be involved in cytoprotective processes and redox signaling. It is very meaningful to track and analyze it in mitochondria. Herein, we report a novel "turn-on" mitochondria-targeting near-infrared fluorescent probe (Mito-NIR-SH) for detection of H2 S in living cells, which was designed and synthesized by introducing 2,4-dinitrophenyl as fluorescence quenching group and H2 S response moiety into Changsha near-infrared fluorophore (CS-OH). The structure of the fluorophore and the probe were characterized by 1 H NMR, 13 C NMR and mass spectrometry...
May 15, 2019: Talanta
Zhenhua Li, Yuping Liu, Xuejin Huang, Changming Hu, Hongwei Wang, Lin Yuan, John L Brash, Hong Chen
Gene transfection, as an effective treatment for inherited and acquired life threatening diseases caused by genetic deficiencies and abnormalities, has evolved as a promising therapeutic strategy for cancer and other intractable diseases. Non-target-specific vectors will affect normal cells as well as pathogenic cells, resulting in a relative decrease in transfection efficiency and unnecessary cytotoxicity. In the present work, gold nanoparticles (AuNPs) functionalized with folate (FA)-modified polyethylenimine (PEI-FA) were prepared by a single step method (without additional reducing agent) for targeted gene transfection in tumor cells...
February 6, 2019: Colloids and Surfaces. B, Biointerfaces
Sascha Tafelski, Doaa Mohamed, Mohammed Shaqura, Chalid Assaf, Antje Beyer, Sascha Treskatsch, Michael Schäfer, Shaaban A Mousa
Evidence is accumulating that activation of mineralocorticoid (MR) and glucocorticoid (GR) receptors on peripheral sensory neurons modulates pain sensation. While the expression and exact anatomical localization of MR and GR in the various subpopulations of peripheral sensory neurons has been shown in animals, it is still unknown for the human skin. Therefore, we aimed to identify MR and GR mRNA and protein as well as the exact subpopulations of sensory neurons in human versus rat skin. Tissue samples from rat and human skin were subjected to RT-PCR, Western blot and double immunofluorescence confocal analysis of MR and GR with the neuronal markers calcitonin gene-related peptide (CGRP), neurofilament 200 (NF200) and tyrosine hydroxylase (TH)...
February 13, 2019: Brain Research
Xiaolan Zhu, Huiling Shen, Xinming Yin, Meiling Yang, Hong Wei, Qi Chen, Fan Feng, Yueqin Liu, Wenlin Xu, Yuefeng Li
BACKGROUND: How exosomal microRNAs (miRNAs) derived from macrophages contribute to the development of drug resistance in the context of the hypoxic tumor microenvironment in epithelial ovarian cancer (EOC) remains poorly understood. METHODS: The miRNA levels were detected by qRT-PCR. Protein levels of HIF-1α, CD163 and PTEN-PI3K/AKT pathway were assessed by Western blot (WB) and Immunohistochemistry (IHC). Exosomes were isolated, and then confirmed by Transmission electron microscopy (TEM), Nanoparticle Tracking Analysis (NTA) and WB...
February 15, 2019: Journal of Experimental & Clinical Cancer Research: CR
Thwe Zin Ei, Yasushi Shimada, Ahmed Abdou, Alireza Sadr, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami
OBJECTIVE: The purpose of this study was to detect and investigate the association of enamel microcracks with demineralization at proximal contact areas of premolars, using 3D swept-source optical coherence tomography (SS-OCT). METHODS: Extracted maxillary and mandibular premolars (n=50 each), without any visible tooth cracks, were examined for demineralization of interproximal contact areas, using the International Caries Detection and Assessment System (ICDAS)...
February 12, 2019: Dental Materials: Official Publication of the Academy of Dental Materials
Kevin Ho, Cristobal Morfin, Katarzyna Slowinska
The in vitro efficacy of cancer prodrugs varies significantly between malignant cell lines. The most commonly identified problems relate to delivery: uptake mechanism, endosomal entrapment, and drug release. Here we present the study of collagen/cell penetrating hybrid (COL/CPP) peptide carriers intended to deliver paclitaxel to the hypopharyngeal carcinoma (FaDu) cells. Confocal microscopy imaging revealed the surprising response of FaDu cell to COL/CPP in comparison to previously studied cancer cell lines: hybrid peptides that carry both COL and CPP domain adsorb on the FaDu cell surface...
February 14, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Marie-Christine Gerbod-Giannone, Laurence Dallet, Grégoire Naudin, Annelise Sahin, Marion Decossas, Sylvie Poussard, Olivier Lambert
Atherosclerosis is a lipid disease characterized by accumulation of low density lipoprotein (LDL) in the artery wall. The transport of LDL across the endothelium of coronary artery is an initiating event of atherosclerosis, whose mechanism remains poorly understood. In the last decade, it has been shown that in caveolin-1 (Cav-1) deficient mice, LDL infiltration in aorta wall is decreased and CD36 expression in aortas is down-regulated, leading to regression of atherosclerotic lesions. In the present study, we show that native LDL endocytosis is decreased in endothelial cells deficient in Cav-1 or CD36...
February 12, 2019: Biochimica et biophysica acta. General subjects
Shouxiang Zhang, Mengjie Liu, Lewis Yi Fong Tan, Quentin Hong, Ze Liang Pow, Tze Cin Owyong, Siyang Ding, Wallace Wing Ho Wong, Yuning Hong
Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide-functionalized tetraphenylethene (TPE)-derivatized fluorescent probe, TPE-NMI, which shows fluorescence turn-on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by using flow cytometry and visualized with confocal microscopy...
February 15, 2019: Chemistry, An Asian Journal
Joshua Ziegler, Rachael Klaiss, Andrew Blaikie, David Joseph Miller, Viva Horowitz, Benjamin J Aleman
Quantum emitters (QEs) in 2D hexagonal boron nitride (hBN) are extremely bright and are stable at high temperature and under harsh chemical conditions. Because of they reside within an atomically thin 2D material, these QEs have a unique potential to couple strongly to hybrid optoelectromechanical and quantum devices. However, this potential for coupling has been underexplored because of challenges in nanofabrication and patterning of hBN QEs. Motivated by recent studies showing that QEs in hBN tend to form at edges, we use a focused ion beam (FIB) to mill an array of patterned holes into hBN...
February 15, 2019: Nano Letters
Jinxin Zhang, Shujie Yang, Chuyi Chen, Jessica H Hartman, Po-Hsun Huang, Lin Wang, Zhenhua Tian, Peiran Zhang, David Faulkenberry, Joel N Meyer, Tony Jun Huang
Controllable, precise, and stable rotational manipulation of model organisms is valuable in many biomedical, bioengineering, and biophysics applications. We present an acoustofluidic chip capable of rotating Caenorhabditis elegans (C. elegans) in both static and continuous flow in a controllable manner. Rotational manipulation was achieved by exposing C. elegans to a surface acoustic wave (SAW) field that generated a vortex distribution inside a microchannel. By selectively activating interdigital transducers, we achieved bidirectional rotation of C...
February 15, 2019: Lab on a Chip
Mio Yoshida, Yuichi Kozawa, Shunichi Sato
We demonstrate that the spatial resolution in confocal laser scanning microscopy is remarkably improved by simple image subtraction between images acquired by higher-order transverse modes of a cylindrical vector beam, referred to as radially and azimuthally polarized Laguerre-Gaussian mode beams. Two types of combinations of vector mode beams suitable for subtraction imaging are derived by a systematical study based on numerical calculations. The spatial resolution of about 100 nm is experimentally achieved without the degradation of image quality...
February 15, 2019: Optics Letters
DeGui Sun, Hongpeng Shang, Huilin Jiang
Surface roughness is one of the important parameters affecting the optical scattering loss of dielectric waveguides. Despite extensive research interests in correlating optical losses with surface roughness, not much research focus has been dedicated to the study of an accurate metrology and measurement standard for the characterization of waveguide roughness. In this Letter, we report an effective metrology for the measurement of waveguide surface roughness, using confocal laser scanning microscopy (CLSM)...
February 15, 2019: Optics Letters
Hongrae Kim, Sunhye Lee, Hyunjin Kim, Hee Jin Chang, Yongdoo Choi, Hong Man Yoon, Myeong-Cherl Kook, So-Youn Jung, Youngmi Kwon, Juehyung Kang, Hongki Yoo, Incheon Song, Seungrag Lee, Dae Kyung Sohn
With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real-time diagnosis during surgery. In this paper, we propose a fluorescence staining method using Hoechst 33342 and Eosin that does not require tissue freezing and slicing...
February 14, 2019: Microscopy Research and Technique
André R S Garraffoni, Thiago Q Araújo, Anete P Lourenço, Loretta Guidi, Maria Balsamo
The order Macrodasyida (Gastrotricha) includes over 350 marine species, and only 3 freshwater species (Marinellina flagellata, Redudasys fornerise, R. neotemperatus). Herein we describe a new freshwater species of Macrodasyida, Redudasys brasiliensis sp. nov., from Brazil through an integrative taxonomic approach. The external morphology and internal anatomy were investigated using differential interference contrast microscopy, confocal microscopy, scanning and transmission electron microscopy. The systematization of the new taxon was inferred by nuclear (18S and 28S) and mitochondrial (COI) genes, and its intra-order relationships were assessed using data from most of available macrodasyids...
February 14, 2019: Scientific Reports
Elisabet Augé, Ingo Bechmann, Núria Llor, Jordi Vilaplana, Martin Krueger, Carme Pelegrí
Corpora amylacea are spherical bodies of unknown origin and function, which accumulate in the human brain during the aging process and neurodegenerative disorders. In recent work, we reported that they contain some neo-epitopes that are recognized by natural IgMs, revealing a possible link between them and the natural immune system. Here, we performed an ultrastructural study complemented with confocal microscopy in order to shed light on the formation of corpora amylacea and to precisely localize the neo-epitopes...
February 14, 2019: Scientific Reports
Samadrita Bhattacharyya, Jialei Duan, Lin Wang, Boxun Li, Minoti Bhakta, Antonio Fernandez-Perez, Gary C Hon, Nikhil V Munshi
The atrioventricular node (AVN) coordinates the timing of atrial and ventricular contraction to optimize cardiac performance. To study this critical function using mouse genetics, however, new reagents are needed that allow AVN-specific manipulation. Here we describe a novel Gjd3-CreEGFP mouse line that successfully recombines floxed alleles within the AVN beginning at E12.5. These mice have been engineered to express CreEGFP under the control of endogenous Gjd3 regulatory elements without perturbing native protein expression...
February 14, 2019: Scientific Reports
Ming-Shih Hwang, Michael G Strainic, Elliot Pohlmann, Elzbieta Pluskota, Diana L Ramirez-Bergeron, Edward F Plow, M Edward Medof
Purified vascular endothelial cell (EC) growth factor receptor-2 (VEGFR2) auto-phosphorylates upon VEGF-A occupation in vitro arguing that VEGR2 confers its mitotic and viability signaling in and of itself. Herein, we show that in ECs, VEGFR2 function requires concurrent C3a/C5a receptor (C3ar1/C5ar1) and IL-6 receptor (IL-6R)-gp130 co-signaling. C3ar1/C5ar1 or IL-6R blockade totally abolished VEGFR2 auto-phosphorylation, downstream Src, ERK, AKT, mTOR, and STAT3 activation, and EC cell cycle entry. VEGF-A augmented production of C3a/C5a/IL-6 and their receptors via a two-step p-Tyk2/p-STAT3 process...
February 14, 2019: Journal of Cell Science
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