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mesenchymal stem cells, stem cells,

Mutsuko Minata, Alessandra Audia, Junfeng Shi, Songjian Lu, Joshua Bernstock, Marat S Pavlyukov, Arvid Das, Sung-Hak Kim, Yong Jae Shin, Yeri Lee, Harim Koo, Kirti Snigdha, Indrayani Waghmare, Xing Guo, Ahmed Mohyeldin, Daniel Gallego-Perez, Jia Wang, Dongquan Chen, Peng Cheng, Farah Mukheef, Minerva Contreras, Joel F Reyes, Brian Vaillant, Erik P Sulman, Shi-Yuan Cheng, James M Markert, Bakhos A Tannous, Xinghua Lu, Madhuri Kango-Singh, L James Lee, Do-Hyun Nam, Ichiro Nakano, Krishna P Bhat
Unresectable glioblastoma (GBM) cells in the invading tumor edge can act as seeds for recurrence. The molecular and phenotypic properties of these cells remain elusive. Here, we report that the invading edge and tumor core have two distinct types of glioma stem-like cells (GSCs) that resemble proneural (PN) and mesenchymal (MES) subtypes, respectively. Upon exposure to ionizing radiation (IR), GSCs, initially enriched for a CD133+ PN signature, transition to a CD109+ MES subtype in a C/EBP-β-dependent manner...
February 12, 2019: Cell Reports
Daniel J Weiss
As the prevalence and impact of lung diseases continue to increase worldwide, new therapeutic strategies are desperately needed. Advances in lung-regenerative medicine, a broad field encompassing stem cells, cell-based therapies, and a range of bioengineering approaches, offer new insights into and new techniques for studying lung physiology and pathophysiology. This provides a platform for the development of novel therapeutic approaches. Applicability to chronic obstructive pulmonary disease of recent advances and applications in cell-based therapies, predominantly those with mesenchymal stromal cell-based approaches, and bioengineering approaches for lung diseases are reviewed...
December 2018: Annals of the American Thoracic Society
Maria Grazia Raucci, Ines Fasolino, Maria Caporali, Manuel Serrano-Ruiz, Alessandra Soriente, Maurizio Peruzzini, Luigi Ambrosio
Nowadays chemotherapy is the main treatment for osteosarcoma disease, even if limited by the lack of selectivity between healthy and cancer cells during the inhibition of cell division. Herein, we propose the use of few-layer black phosphorous (2D bP) as an alternative tool for osteosarcoma treatment and report how 2D bP can stimulate newly forming bone tissue generation after osteosarcoma resection. In our study, we have developed an in vitro model to evaluate the efficacy of 2D bP material with and without near-infrared light irradiation treatment and we have demonstrated that the presence of 2D bP without treatment inhibits the metabolic activity of osteosarcoma cells (SAOS-2), while inducing both the proliferation and the osteogenic differentiation of human pre-osteoblast cells (HOb) and mesenchymal stem (hMSC) cells...
February 13, 2019: ACS Applied Materials & Interfaces
Ayda Pouyafar, Aysa Rezabakhsh, Reza Rahbarghazi, Milad Zadi Heydarabad, Elhameh Shokrollahi, Emel Sokullu, Majid Khaksar, Alireza Nourazarian, Çığır Biray Avci
In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in endothelial induction medium containing sulindac and resveratrol. Expression of endothelial markers such as the von Willebrand factor (vWF) and vascular endothelial cadherin (VE-cadherin) and genes participating in mesenchymal-to-epithelial transition was studied by real-time PCR assay...
February 13, 2019: Cell and Tissue Research
Weerachai Singhatanadgit, Montree Toso, Boonsong Pratheepsawangwong, Alongkorn Pimpin, Werayut Srituravanich
Long-term clinical success of a titanium implant not only depends upon osseointegration between implant and bone surface but also on the response of host immune cells. Following implantation of biomaterials, an inflammatory response, including T lymphocyte response, is ostensibly initiated by implant-cell interaction. However, little is known about the responses of T lymphocytes to titanium dioxide nanotubes. The present study aimed to explore the effect of titanium dioxide nanotubes on T lymphocytes in vitro and its biological consequences...
February 2019: Journal of Biomaterials Applications
Jie Gu, Yuxia Wang, Juan Gao, Shengnan Yuan, Yajing Chu, Yanhan Li, Weiping Yuan, Xiaomin Wang
Tet2 (member 2 of the Tet family) plays an important role in DNA demethylation modification, epigenetic regulation, and hematopoiesis. In our previous study, we found that Tet2 knockout mice progressively developed lymphocytic leukemia and myeloid leukemia with aging. However,the role of Tet2 in bone marrow microenvironment is unclear. Here in this study, we found that more Tet2-/- mesenchymal stem cells (MSCs) from bone marrow were in the G2/M cell cycle stages. The division time of Tet2-/- MSCs was shorter than that of the control cells...
January 25, 2019: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Zhong-Bao Ruan, Ge-Cai Chen, Rui Zhang, Li Zhu
So far, there were no reports on circular RNA (circRNA) expression profiles in the differentiation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte-like cells induced by 5-aza. In this study, hUCMSCs were isolated from umbilical cords and induced with 5-aza for 14 days. Immunofluorescence staining, real-time reverse transcription polymerase chain reaction (RT-PCR), and western blot of cardiac troponin I and α-sarcomeric actin on hUCMSCs between Days 14 and 0 were performed...
February 12, 2019: Journal of Cellular Physiology
Seong Hwan Kim, Chul-Won Ha, Yong-Beom Park, Eunwoo Nam, Jung-Eun Lee, Han-Jun Lee
INTRODUCTION: Mesenchymal stem cells (MSCs) have gained popularity for articular cartilage repair. However, efficacy of intra-articular MSCs in osteoarthritis remains unclear. In the setting of a meta-analysis of randomized controlled trials (RCTs), we aimed to investigate the efficacy of intra-articular MSCs on clinical outcomes and cartilage repair in patients with knee osteoarthritis. MATERIALS AND METHODS: PubMed, EMBASE, Cochrane Library, CINAHL, and Scopus were searched from inception to March 31, 2017...
February 11, 2019: Archives of Orthopaedic and Trauma Surgery
Min Hu, Wenzheng Guo, Yueling Liao, Dongliang Xu, Beibei Sun, Hongyong Song, Tong Wang, Yanbin Kuang, Bo Jing, Kaimi Li, Jing Ling, Feng Yao, Jiong Deng
Induction of cancer stem cell (CSC) characters and epithelial mesenchymal transition (EMT) features are crucial in tumor initiation, progression and metastasis. However, underlying mechanisms remain incompletely understood. Here, we showed that ENPP1 plays an important role in inducing and maintaining EMT phenotypes and CSC features in lung cancer. ENPP1 is upregulated in lung cancer cells. ENPP1-knockdown in lung cancer HCC827 cells and A549 cells resulted in suppressed colonogenic formation, anchorage-independent growth in vitro, and tumorigenicity in vivo...
2019: American Journal of Cancer Research
Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco
Type 2 diabetes (T2D) is a major metabolic disease and a key epigenetic risk factor for the development of additional clinical complications. Among them, periodontitis (PD), a severe inflammatory disease ascribable to a dysregulated physiology and composition of the oral microbiota, represents one of the most relevant complications. Periodontitis can impact the structure of the tooth and likely the stem and progenitor cell pool, which actively contributes to the periodontal microenvironment and homeostasis...
2019: Stem Cells International
Zucheng Luo, Fangfang Wu, Enxing Xue, Linlin Huang, Ping Yan, Xiaoyun Pan, Yulong Zhou
Bone marrow derived stem cells (BMSCs) transplantation are viewed as a promising therapeutic candidate for spinal cord injury (SCI). However, the inflammatory microenvironment in the spinal cord following SCI limits the survival and efficacy of transplanted BMSCs. In this study, we investigate whether injured neuronal cells derived exosomes would influence the survival of transplanted BMSCs after SCI. In order to mimic the microenvironment in SCI that the neuronal cells or transplanted BMSCs suffer in vivo, PC12 cells conditioned medium and PC12 cell's exosomes collected from H2 O2 -treated PC12 cell's culture medium were cultured with BMSCs under oxidative stress in vitro...
February 12, 2019: Cell Death & Disease
Weijie Zhong, Zhigang Zhu, Xin Xu, Hui Zhang, Huabao Xiong, Qingshan Li, Yaming Wei
BACKGROUND: The drug-resistance and relapse of diffuse large B-cell lymphoma (DLBCL), which are related to mesenchymal stem cells (MSCs), have become increasingly common. However, the underlying mechanisms remain elusive. METHODS: CCK 8 assay, colony formation assay, and xenograft mouse model were used to investigate the effects of hBMSCs on DLBCL growth. Immunohistochemistry, qRT-PCR, and ELISA were used to study the expressions of IL-6 and IL-17A. Flow cytometry was used to analyze Th17 cells and Treg cells expressions...
February 12, 2019: Journal of Experimental & Clinical Cancer Research: CR
Tomasz Kolenda, Kacper Guglas, Anna Teresiak, Renata Bliźniak, Katarzyna Lamperska
INTRODUCTION: Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of epithelial-to-mesenchymal transition (EMT). However, it is unclear if let-7d and miR-205 together influence cancer cells. AIM: To determine if let-7d and miR-205 expression levels influence HNSCC patient outcome...
February 13, 2019: Journal of Biomedical Science
Atefeh Pesaraklou, Nasser Mahdavi-Shahri, Halimeh Hassanzadeh, Masoud Ghasemi, Mahboubeh Kazemi, Nasser Sanjar Mousavi, Maryam M Matin
Today advancements in nanotechnology have made extensive progress in tissue engineering. Application of cerium oxide nanoparticles (CeO2) has improved regenerative medicine due to their antioxidant properties. In this study, nanoparticles were used to increase the efficacy of skin substitutes. Human skin samples were decellularized using four methods and studied via histological stainings and DNA content analyses. Then CeO2 dispersing and its stability were investigated. The prepared acellular dermal matrices (ADMs) were immersed in CeO2 suspension and their effects were evaluated on growth of cultured human adipose derived-mesenchymal stem cells (hAd-MSCs) using MTT assay and histological methods...
February 12, 2019: Biomedical Materials
Miina Ojansivu, Ahmad Rashad, Astrid Elisabet Ahlinder, Jonathan Massera, Ayush Mishra, Kristin Syverud, Anna Finne-Wistrand, Susanna Miettinen, Kamal Mustafa
A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was to evaluate the influence of wood-based cellulose nanofibrils (CNF) and bioactive glass on the rheological properties of gelatin-alginate bioinks and the initial responses of bone cells embedded in these inks. CNF modulated the flow behavior of the hydrogels, thus improving their printability. Chemical characterization by SEM-EDX and ion release analysis confirmed the reactivity of the BaG in the hydrogels...
February 12, 2019: Biofabrication
Bin Lu, Dao-Yu Zhu, Jun-Hui Yin, He Xu, Changqing Zhang, Qin-Fei Ke, You-Shui Gao, Ya-Ping Guo
Hierarchically porous structures and bioactive compositions of artificial biomaterials play a positive role in bone defect healing and new bone regeneration. Herein, cerium oxide nanoparticles-modified bioglass (Ce-BG) scaffolds were firstly constructed by the incorporation of hollow mesoporous Ce-BG microspheres in CTS via a freeze-drying technology. The interconnected macropores in Ce-BG scaffolds facilitated the in-growth of bone cells/tissues from material surfaces into the interiors, while the hollow cores and mesopore shells in Ce-BG microspheres provides more active sites for bone mineralization...
February 12, 2019: Biofabrication
Jinxuan Zhao, Xueling Li, Jiaxin Hu, Fu Chen, Shuaihua Qiao, Xuan Sun, Ling Gao, Jun Xie, Biao Xu
Aims: Mesenchymal stem cells (MSCs) gradually become attractive candidates for cardiac inflammation modulation, yet understanding of the mechanism remains elusive. Strikingly, recent studies indicated that exosomes secreted by MSCs might be a novel mechanism for the beneficial effect of MSCs transplantation after myocardial infarction. We therefore explored the role of MSC-derived exosomes (MSC-Exo) in the immunomodulation of macrophages after myocardial ischemia/reperfusion (I/R) and its implications in cardiac injury repair...
February 8, 2019: Cardiovascular Research
Deborah Stanco, Christian Caprara, Gianluca Ciardelli, Luca Mariotta, Mauro Gola, Greta Minonzio, Gianni Soldati
Adipose-derived stem cells (ASCs) are multipotent and immune-privileged mesenchymal cells, making them ideal candidates for therapeutic purposes to manage tendon disorders. Providing safe and regulated cell therapy products to patients requires adherence to good manufacturing practices. To this aim we investigated the in vitro tenogenic differentiation potential of ASCs using a chemically defined serum-free medium (SF) or a xenogenic-free human pooled platelet lysate medium (hPL) suitable for cell therapy and both supplemented with CTGF, TGFβ-3, BMP-12 and ascorbic acid (AA) soluble factors...
2019: PloS One
Olivia Kérourédan, Jean-Michel Bourget, Murielle Rémy, Sylvie Crauste-Manciet, Jérôme Kalisky, Sylvain Catros, Noëlie B Thébaud, Raphaël Devillard
Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells...
February 12, 2019: Journal of Materials Science. Materials in Medicine
Hanaa H Ahmed, Hadeer A Aglan, Mostafa Mabrouk, Ahmed A Abd-Rabou, Hanan H Beherei
The main target of this work was to explore the proliferative impact of selenium dioxide nanoparticles (SeO2 ) and selenium dioxide/titanium dioxide nanocomposites (Se/Ti (I), (II) and (III)) on mesenchymal stem cells (MSCs). For this purpose, SeO2 and Se/Ti (I), (II) and (III) were prepared by facile one step method and characterized by transmission electron microscopy (TEM), Zetasizer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) along with energy-dispersive X-ray spectrometry (EDX) with reference to SeO2 nanoparticles...
February 12, 2019: Journal of Materials Science. Materials in Medicine
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