Mirella Belleri, Giuseppe Paganini, Daniela Coltrini, Roberto Ronca, Daniela Zizioli, Michela Corsini, Andrea Barbieri, Elisabetta Grillo, Stefano Calza, Roberto Bresciani, Eugenio Maiorano, Mauro G Mastropasqua, Tiziana Annese, Arianna Giacomini, Domenico Ribatti, Josefina Casas, Thierry Levade, Gemma Fabrias, Marco Presta
Disturbance of sphingolipid metabolism may represent a novel therapeutic target in metastatic melanoma, the most lethal form of skin cancer. Beta-galactosylceramidase (GALC) removes beta-galactose from galactosylceramide and other sphingolipids. In this study, we show that downregulation of galcb, a zebrafish orthologue of human GALC, affects melanoblast and melanocyte differentiation in zebrafish embryos, suggesting a possible role for GALC in melanoma. On this basis, the impact of GALC expression in murine B16-F10 and human A2058 melanoma cells was investigated following its silencing or upregulation...
September 30, 2020: Cancer Research