Jianping Zhang, Jayaveeramuthu Nirmala, Shisheng Chen, Matthias Jost, Burkhard Steuernagel, Mirka Karafiatova, Tim Hewitt, Hongna Li, Erena Edae, Keshav Sharma, Sami Hoxha, Dhara Bhatt, Rea Antoniou-Kourounioti, Peter Dodds, Brande B H Wulff, Jaroslav Dolezel, Michael Ayliffe, Colin Hiebert, Robert McIntosh, Jorge Dubcovsky, Peng Zhang, Matthew N Rouse, Evans Lagudah
Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h. Each allele encodes a highly related nucleotide-binding site leucine-rich repeat (NB-LRR) type immune receptor, containing an unusual long LRR domain, that confers resistance to a unique spectrum of isolates of the wheat stem rust pathogen...
November 14, 2023: Nature Communications