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Complement c4

Yuqing Lu, Liangqiang Zhou, Tejvansh Shenoy Imrit, Aiguo Liu
OBJECTIVE: To investigate the clinical characteristics, etiology, treatment outcomes, and prognostic factors of sudden sensorineural hearing loss (SSNHL) in children to guide the clinical diagnosis and treatment of SSNHL in the pediatric population. STUDY DESIGN: Retrospective case review. SETTING: Tertiary referral center. PATIENTS: Patients diagnosed with SSNHL from November 2011 to December 2017 with relatively complete clinical data...
April 2019: Otology & Neurotology
Yoshihisa Shimizu, Kiyoshi Ichihara
Background Many reports address the stability of biochemical analytes in serum. However, studies covering a wide range of storage temperatures are unavailable. Using equipment enabling precise temperature control, we investigated the effect of six different storage temperatures on serum analytes. Methods Serum specimens from seven healthy volunteers were obtained and divided into multiple aliquots for storage at -30, -20, -10, 0, 4, and 25 °C. On days 1, 3, 7, 14, 28 and 56, the aliquots stored at each temperature were relocated to a deep freezer maintained at -80 °C...
March 12, 2019: Clinical Chemistry and Laboratory Medicine: CCLM
Ingyu Park, Kyeongho Lee, Kausik Bishayee, Hong Jin Jeon, Hyosang Lee, Unjoo Lee
Scratching is a main behavioral response accompanied by acute and chronic itch conditions, and has been quantified as an objective correlate to assess itch in studies using laboratory animals. Scratching has been counted mostly by human annotators, which is a time-consuming and laborious process. It has been attempted to develop automated scoring methods using various strategies, but they often require specialized equipment, costly software, or implantation of device which may disturb animal behaviors. To complement limitations of those methods, we have adapted machine learning-based strategy to develop a novel automated and real-time method detecting mouse scratching from experimental movies captured using monochrome cameras such as a webcam...
February 2019: Experimental Neurobiology
Johannes von Kempis, Sabine Duetsch, Nicola Reuschling, Rahel Villiger, Peter M Villiger, Florence Vallelian, Dominik J Schaer, Ruediger B Mueller
AIMS OF THE STUDY: To describe patterns of systemic lupus erythematosus (SLE) care and the clinical effectiveness of belimumab plus standard of care therapy in a real-world clinical setting in Switzerland. METHODS: This multicentre, observational, retrospective cohort study included adults with SLE who initiated belimumab as part of their usual care at least six months before data analysis. The primary outcome was the overall clinical response, assessed by a physician on a Physician’s Global Assessment-like scale, to six months’ treatment with belimumab...
February 25, 2019: Swiss Medical Weekly
Raúl Teruel-Montoya, Ginés Luengo-Gil, Fernando Vallejo, José Enrique Yuste, Nataliya Bohdan, Nuria García-Barberá, Salvador Espín, Constantino Martínez, Juan Carlos Espín, Vicente Vicente, Irene Martínez-Martínez
Paroxysmal Nocturnal Hemoglobinuria (PNH) is a clonal disease of blood cells caused by the lack of glycosyl phosphatidyl inositol anchored proteins bound to the cell membrane. In consequence, erythrocytes lead to intravascular hemolysis upon complement activation, which promotes high risk of thrombosis, intravascular hemolytic anemia, and bone marrow failure in patients. The mechanisms of thrombosis in PNH are still poorly understood. Treatment with eculizumab reduces intravascular hemolysis and thrombotic risk, but not in all cases...
March 5, 2019: Scientific Reports
Maria Maślińska, Małgorzata Mańczak, Brygida Kwiatkowska
INTRODUCTION: The rheumatoid factor (RF) is present in numerous autoimmune disorders, although its role in many of them remains a subject of research. The study assesses the role of RF as an immunological and prognostic factor in the primary Sjögren's syndrome (pSS). METHODS: Seventy-five pSS patients (mean age 50.03 ± 15.1), 65 (87%) females, and 10 (13%) males. WBC, CRP, RF, ESR, gammaglobulins, C4, C3 component of complement, cryoglobulins, ANA, anti-SS-A, and anti-SS-B antibodies were determined...
February 27, 2019: Clinical Rheumatology
Abdulaziz Almutairi, Ziyad Alkathiri, Sulaiman M Al-Mayouf
Background: Children with lupus nephritis particularly, diffuse proliferative and membranous glomerulonephritis, may necessitate potent immunosuppressive medications and occasionally combined therapy. Objective: To report the beneficial effects of tacrolimus (TAC) in children with refractory lupus nephritis from a single tertiary pediatric rheumatology clinic. Methods: This is a retrospective case series of children with refractory lupus nephritis treated with TAC after failure of aggressive immunosuppressive treatment...
September 2018: International Journal of Pediatrics & Adolescent Medicine
Yanxia Gong, Wei Niu, Yanping Tang, Qingyu Zhang, Simiao Liu, Xi Liu, Xiao Wang, Yang Xu
The aim of the present study was to determine the influence of stress on the colonic mucosa and immune system and to further investigate the association between stress and development and pathogenesis of ulcerative colitis (UC). Mice were treated with 2,4,6-trinitrobenzenesulfonic acid to induce an animal model of UC, and stress was induced by water immersion and restraint. Subsequently, the disease activity index (DAI), secretory immunoglobulin A (sIgA), IgA, interleukin (IL)-6 and -8, tumor necrosis factor-α (TNF-α), complement component (C)3 and C4, and alterations in the colonic mucosa were observed...
March 2019: Experimental and Therapeutic Medicine
Mai Kawazoe, Kaichi Kaneko, Zento Yamada, Shotaro Masuoka, Satoshi Mizutani, Soichi Yamada, Kotaro Shikano, Hiroshi Sato, Makoto Kaburaki, Sei Muraoka, Shinichi Kawai, Toshihiro Nanki
OBJECTIVES: To assess the renal and non-renal efficacy of mycophenolate mofetil (MMF) in Japanese patients with systemic lupus erythematosus (SLE). METHODS: We conducted a retrospective study to assess the renal and non-renal efficacies of MMF in Japanese patients with systemic lupus erythematosus (SLE). We analyzed 14 patients with lupus nephritis (LN) who were given MMF, and 13 patients who received monthly intravenous cyclophosphamide (IVCY) as induction therapy, and a further 19 patients without LN who were treated with MMF, and 13 patients who took tacrolimus (TAC) to reduce glucocorticoid dosages...
February 18, 2019: Clinical Rheumatology
Li Chen, Yipeng Zhang
This study aims to investigate the effects of dietary Porphyra yezoensis polysaccharides (PPs) on growth performance and nonspecific immunity of juvenile grass carp (Ctenopharyngodon idella). Four hundreds of juvenile grass carps were randomly divided into four groups, i.e. one control group received basal diet and three treatment groups received diets supplemented with three different levels of PPs (1, 3, and 5 g kg-1 ) and tested for growth performance, survival percent, intestine digestive-enzyme activities, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione peroxidase (GPx) activity, serum total protein, lysozyme, alkaline phosphatase, complement C3 and complement C4 and resistance to Aeromonas hydrophila of juvenile grass carp...
February 9, 2019: Fish & Shellfish Immunology
Mu-Yang Li, Xin-Ming Zhu, Xiao-Tian Niu, Xiu-Mei Chen, Jia-Xin Tian, Yi-Di Kong, Dong-Ming Zhang, Lei Zhao, Gui-Qin Wang
The present study evaluated the effects of dietary Allium mongolicum Regel polysaccharide (AMRP) on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus. A basal diet was supplemented with AMRP at 0, 1, 1.5 or 2 g/kg feed for 56 days. After the 56 days feeding period, weight gain (WG), specific growth rate (SGR) and feed conversion ratio (FCR) were significantly increased or decreased (P < 0.05) by dietary AMRP, with the highest WG, SGR and the minimum FCR occurring in 1...
February 12, 2019: Molecular Biology Reports
Jiaxi Wang, Jie Li, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
The highly effective analysis of glycopeptides from complex biological samples is an attractive and critical topic all the time. In this study, a novel thickness-controlled hydrophilic Mg-metal organic frameworks (Mg-MOFs) coating-functionalized magnetic graphene composite (MagG@Mg-MOFs-1C) was prepared for the capture of the glycopeptides. The as-synthesized composite exhibits an ultralow limit of detection (0.1 fmol μL-1), a perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 500 : 500, w/w/w), and a high binding capacity (150 mg g-1), satisfying reusability and high recovery in the recognition of glycopeptides due to its outstanding characteristics including strong magnetic property, large surface area (617 m2 g-1), plenty of affinity sites, and excellent hydrophilicity...
February 11, 2019: Nanoscale
Rui Marinho, António Alves, Nuno Pignatelli, Vítor Nunes
A 24-year-old black male presented with a 1-week obstructive jaundice and intermittent abdominal pain, with no significant weight loss and an unsuspicious abdominal exam. Blood chemistry showed a cholestatic pattern but a complete immunological and tumoral panel (anti-smooth muscle antibody, anti-mitochondrial antibody, anti-nuclear antibody, anti-neutrophil cytoplasmic antibody, anti-Smith, anti-double-stranded-DNA antibody (anti-dsDNA), complement C3/C4, carcinoembryonic antigen, CA 19-9 and IgG4) were all within normal limits...
January 2019: Journal of Surgical Case Reports
M Feng, S L Zhang, Z J Liang, Y L Wang, X C Zhao, C Gao, H Guo, J Luo
OBJECTIVE: To investigate the diagnostic role of complement C3, complement C4, C-reactive protein (CRP), procalcitonin (PCT), white blood cell count (WBC), neutrophil CD64 (nCD64) index, lymphocyte subsets and their combination in differentiating bacterial infection from disease relapse in systemic lupus erythematosus (SLE). METHODS: The above biomarkers in 36 hospitalized SLE patients with bacterial infection and 45 with lupus flare without infection were retrospectively studied...
February 2, 2019: Lupus
Marta Monedero, José A Infantes-Lorenzo, Inmaculada Moreno, Ana Belén Martín, Rocio I R Macias, Alfredo Toraño, Mercedes Domínguez
To study complement function in mammalian leishmanioses, we developed mouse monoclonal antibodies to the human complement system components C1q, C4, factor D, factor H, factor B, properdin, C5 and C9. Antibody specificity was determined by indirect and capture ELISA and by Western blot. In flow cytometry analysis, seven antibodies recognized the cognate component on human serum-opsonized Leishmania promastigotes. Antibody reactivity was screened against promastigotes opsonized with sera of nine mammalian genera: pig, guinea pig, goat, rabbit, cat, dog, hamster, jird and rat...
February 2019: Comparative Immunology, Microbiology and Infectious Diseases
Yoshinori Endo, Kazuhiro Hasegawa, Ryo Nomura, Hidetaka Arishima, Ken-Ichiro Kikuta, Taro Yamashita, Yasuteru Inoue, Mitsuharu Ueda, Yukio Ando, Mark R Wilson, Tadanori Hamano, Yasunari Nakamoto, Hironobu Naiki
Sporadic cerebral amyloid angiopathy (CAA) is characterized by cerebrovascular amyloid-β (Aβ) deposition, which leads to lobar hemorrhage and dementia. Biological molecules affecting the development of CAA have not been fully characterized. In this study, we performed proteome analysis of biopsied leptomeningeal and cortical vessels obtained from 6 CAA patients and 5 non-CAA patients who underwent surgery for large lobar hemorrhages. We found that 6 proteins, including Aβ, apolipoprotein E (apoE), clusterin (CLU), albumin, complement C4 and vitronectin were significantly upregulated in the vessels of CAA patients as compared to non-CAA patients...
January 28, 2019: Acta Neuropathologica Communications
Francianne M Amorim, Maurício L Nobre, Larissa S Nascimento, Alesson M Miranda, Glória R G Monteiro, Francisco P Freire-Neto, Maria do Carmo Palmeira Queiroz, José W Queiroz, Malcolm S Duthie, Marcos R Costa, Steven G Reed, Warren D Johnson, Kathryn M Dupnik, Selma M B Jeronimo
BACKGROUND: Leprosy is a treatable infectious disease caused by Mycobacterium leprae. However, there is additional morbidity from leprosy-associated pathologic immune reactions, reversal reaction (RR) and erythema nodosum leprosum (ENL), which occur in 1 in 3 people with leprosy, even with effective treatment of M. leprae. There is currently no predictive marker in use to indicate which people with leprosy will develop these debilitating immune reactions. Our peripheral blood mononuclear cell (PBMC) transcriptome analysis revealed that activation of the classical complement pathway is common to both RR and ENL...
January 28, 2019: PLoS Neglected Tropical Diseases
Changhua Qu, Juan Zhang, Xiumei Zhang, Jiexin Du, Baifang Su, Hong Li
Combined detection of antinuclear antibody (ANA), anti-double-stranded DNA (ds-DNA) antibody and complements C3 and C4 in the diagnosis of systemic lupus erythematosus (SLE) was analyzed. One hundred and ninety-four patients with SLE admitted to Yantaishan Hospital of Yantai from January 2012 to December 2017 were selected as SLE group. A total of 106 patients with non-SLE rheumatic disease were selected as disease control group and 120 healthy subjects as healthy control group. The ANA and anti-ds-DNA antibodies were detected by ELISA and complement C3 and C4 were detected by rate nephelometry...
February 2019: Experimental and Therapeutic Medicine
Travis Mackoy, Nicholas A Mauro, Ralph Allen Wheeler
Static structure factors (S(q)) for many ionic liquids show low-wavenumber peaks whose intensities increase with increasing temperature. The greater peak intensities might seem to indicate increasing intermediate-range order with increasing temperature. Molecular dynamics (MD) simulations for a representative ionic liquid, 1-butyl-1-methyl-pyrrolidinium bis(trifluoromethylsulfonyl)imide (C4 C1 pyrrTFSI), were used to calculate S(q) and partial S(q) (cation-cation, anion-anion, and cation-anion) at 298 K, 363 K, and 500 K...
January 23, 2019: Journal of Physical Chemistry. B
Y C Liang, Y Yao, R J Zhang, M Shao, X L Sun, G X Shi, C Gao, D Yu, J He
Objective: To investigate the role of T follicular helper (Tfh) subsets and T follicular helper effector memory (Tfhem) cells in circulation of patients with systemic lupus erythematosus (SLE), and explore their roles in SLE disease activity index as biomarkers. Methods: This study enrolled 64 patients with SLE and 15 healthy controls. In peripheral blood from patients with SLE and health controls, the percentage of Tfhem (CD3(+)CD4(+)CD45RA(-)CXCR5(+)CCR7(low)PD-1(high)) cells, Tfh (CD3(+)CD4(+)CD127(high)CD25(l)ow CD45RA(-)CXCR5(+)) subset: Tfh1 (CXCR3(+)CCR6(-)Tfh), Tfh2 (CXCR3(-)CCR6(+) Tfh), Tfh17 (CXCR3(-)CCR6(+) Tfh), were detected by flow cytometry...
January 15, 2019: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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