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Valérie Guillet, Patricia Bordes, Cécile Bon, Julien Marcoux, Virginie Gervais, Ambre Julie Sala, Suzana Dos Reis, Nawel Slama, Israel Mares-Mejía, Anne-Marie Cirinesi, Laurent Maveyraud, Pierre Genevaux, Lionel Mourey
SecB chaperones assist protein export by binding both unfolded proteins and the SecA motor. Certain SecB homologs can also control toxin-antitoxin (TA) systems known to modulate bacterial growth in response to stress. In such TA-chaperone (TAC) systems, SecB assists the folding and prevents degradation of the antitoxin, thus facilitating toxin inhibition. Chaperone dependency is conferred by a C-terminal extension in the antitoxin known as chaperone addiction (ChAD) sequence, which makes the antitoxin aggregation-prone and prevents toxin inhibition...
February 15, 2019: Nature Communications
Celia van der Merwe, Neda Jahanshad, Josh W Cheung, Mary Mufford, Nynke A Groenewold, Nastassja Koen, Rajkumar Ramesar, Shareefa Dalvie, James A Knowles, Derrek P Hibar, Caroline M Nievergelt, Karestan C Koenen, Israel Liberzon, Kerry J Ressler, Sarah E Medland, Rajendra A Morey, Paul M Thompson, Dan J Stein
BACKGROUND: There have been considerable recent advances in understanding the genetic architecture of anxiety disorders and posttraumatic stress disorder (PTSD), as well as the underlying neurocircuitry of these disorders. However, there is little work on the concordance of genetic variations that increase risk for these conditions, and that influence subcortical brain structures. We undertook a genome-wide investigation of the overlap between the genetic influences from single nucleotide polymorphisms (SNPs) on volumes of subcortical brain structures and genetic risk for anxiety disorders and PTSD...
February 15, 2019: Journal of Affective Disorders
Yu-Ting Zhuang, Rui Jiang, De-Fu Wu, Yong-Liang Yu, Jian-Hua Wang
It remains a real challenge to treat the industrial wastewater containing hardly-decomposed dyes and heavy metal ions. Herein, an environment-friendly hybrid hydrogel is prepared by a simple mixing of selenocarrageenan (SeCA) and graphene oxide (GO) under heating and stirring for 5 min without any other reagent. Such a selenocarrageenan hybrid graphene hydrogel (SeCA-GH) without volume shrinkage exhibits excellent adsorption capacity and selective binding to dyes and Hg2+ . The maximum adsorption capacity of methylene blue and Hg2+ on SeCA-GH is 168 mg g-1 and 331 mg·g-1 at 25 °C, respectively...
January 16, 2019: Journal of Colloid and Interface Science
Ali M Al-Subhi, Saskia A Hogenhout, Rashid A Al-Yahyai, Abdullah M Al-Sadi
Typical symptoms of phytoplasma infection were observed on 11 important crops in Oman that included alfalfa, sesame, chickpea, eggplant, tomato, spinach, rocket, carrot, squash, field pea, and faba bean. To identify the phytoplasmas in these crops, samples from infected and asymptomatic plants were collected, followed by amplifying and sequencing of the 16S ribosomal RNA, secA, tuf, imp, and SAP11 genes. We found that these sequences share >99% similarity with the peanut witches' broom subgroup (16SrII-D)...
March 2018: Plant Disease
Sri Karthika Shanmugam, Nicholas Backes, Yuanyuan Chen, Alexandra Belardo, Gregory J Phillips, Ross E Dalbey
Different attributes of membrane protein substrates have been proposed and characterized as translocation-pathway determinants. However, several gaps in our understanding of the mechanism of targeting, insertion and assembly of inner membrane proteins exist. Specifically, the role played by hydrophilic N-terminal tails in pathway selection is unclear. In this study we have evaluated length and charge density as translocase determinants using model proteins. Strikingly, the 36 residue N-tail of 2Pf3-Lep translocates independent of YidC-Sec...
January 9, 2019: Journal of Molecular Biology
John Young, Franck Duong
During post-translational translocation in E. coli, polypeptide substrates are driven across the membrane through the SecYEG protein conducting channel using the ATPase SecA, which binds to SecYEG and couples nucleotide hydrolysis to polypeptide movement. Recent studies suggest that SecA is a highly dynamic enzyme, able to repeatedly bind and dissociate from SecYEG during substrate translocation, yet other studies indicate that these dynamics - here referred to as "SecA processivity" - are not a requirement for transport...
January 2, 2019: Journal of Biological Chemistry
Robin A Corey, Zainab Ahdash, Anokhi Shah, Euan Pyle, William John Allen, Tomas Fessl, Janet E Lovett, Argyris Politis, Ian Collinson
Transport of proteins across membranes is a fundamental process, achieved in every cell by the 'Sec' translocon. In prokaryotes, SecYEG associates with the motor ATPase SecA to carry out translocation for pre-protein secretion. Previously, we proposed a Brownian ratchet model for transport, whereby the free energy of ATP-turnover favours the directional diffusion of the polypeptide [Allen et al. eLife 2016]. Here, we show that ATP enhances this process by modulating secondary structure formation within the translocating protein...
January 2, 2019: ELife
Susana Seca, Diana Miranda, Daniela Cardoso, Bernice Nogueira, Henry J Greten, António Cabrita, Manuel Alves Rodrigues
OBJECTIVE: To identify and synthesize the most recent available evidence of effectiveness of acupuncture on pain, physical function and health-related quality of life (HRQoL) in patients with rheumatoid arthritis (RA). METHODS: A comprehensive search of 12 Western and Chinese databases was undertaken from their inception up to end of 2016. Randomized controlled trials (RCTs), concerning patients with RA treated with needle acupuncture, written in English, Portuguese, German or Chinese were included...
December 19, 2018: Chinese Journal of Integrative Medicine
Niels Vandenberk, Spyridoula Karamanou, Athina G Portaliou, Valentina Zorzini, Johan Hofkens, Jelle Hendrix, Anastassios Economou
SecA converts ATP energy to protein translocation work. Together with the membrane-embedded SecY channel it forms the bacterial protein translocase. How secretory proteins bind to SecA and drive conformational cascades to promote their secretion remains unknown. To address this, we focus on the preprotein binding domain (PBD) of SecA. PBD crystalizes in three distinct states, swiveling around its narrow stem. Here, we examined whether PBD displays intrinsic dynamics in solution using single-molecule Förster resonance energy transfer (smFRET)...
October 29, 2018: Structure
Nagaraju Chada, Kanokporn Chattrakun, Brendan P Marsh, Chunfeng Mao, Priya Bariya, Gavin M King
SecA is the critical adenosine triphosphatase that drives preprotein transport through the translocon, SecYEG, in Escherichia coli . This process is thought to be regulated by conformational changes of specific domains of SecA, but real-time, real-space measurement of these changes is lacking. We use single-molecule atomic force microscopy (AFM) to visualize nucleotide-dependent conformations and conformational dynamics of SecA. Distinct topographical populations were observed in the presence of specific nucleotides...
October 2018: Science Advances
Djenisa H A Rocha, Ana M L Seca, Diana C G A Pinto
Isolation, finding or discovery of novel anticancer agents is very important for cancer treatment, and seaweeds are one of the largest producers of chemically active metabolites with valuable cytotoxic properties, and therefore can be used as new chemotherapeutic agents or source of inspiration to develop new ones. Identification of the more potent and selective anticancer components isolated from brown, green and red seaweeds, as well as studies of their mode of action is very attractive and constitute a small but relevant progress for pharmacological applications...
October 26, 2018: Marine Drugs
Axel Andres, Graziano Oldani, Thierry Berney, Philippe Compagnon, Pål-Dag Line, Christian Toso
Liver transplantation (LT) has become the standard of care for selected primary and secondary malignancies. Considered a contra-indication to transplantation until recently, unresectable colorectal liver metastases (CRLM) have gained interest since the publication of the SECA trial by the University of Oslo. It showed a 5-year overall survival of 60%, comparable to the one of standard transplant indication. This report generated multiple questions about the place of LT for CRLM and gave raise to several trials aiming at answering them...
2018: Translational Gastroenterology and Hepatology
Susana Seca, Miguel Patrício, Sebastian Kirch, Giovanna Franconi, António S Cabrita, Henry J Greten
BACKGROUND: Rheumatoid arthritis (RA) is characterized by pain, functional disability, poor quality of life (QoL), high socioeconomic impact, and annual costs of over $56 billion in the United States. Acupuncture (AC) is widely in use; however, studies show severe methodological shortcomings, did not consider the functional diagnosis for the allocation of acupoints and their results showed no differences between verum and control groups. OBJECTIVE: The authors aimed to objectively assess the safety and efficacy of AC treatments for RA...
October 16, 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
Donald Oliver
Characterization of Sec-dependent bacterial protein transport has often relied on an in vitro protein translocation system comprised in part of E. coli inverted inner membrane vesicles or more recently purified SecYEG translocons reconstituted into liposomes using mostly a single substrate (proOmpA). A paper published in this issue of the Journal of Bacteriology (P. Bariya and L. Randall, J Bacteriol, this issue) finds that inclusion of SecA protein during SecYEG proteoliposome reconstitution dramatically improves the number of active translocons: both an experimentally useful and intriguing result that may arise from SecA membrane integration properties as discussed here...
October 15, 2018: Journal of Bacteriology
Kaitlin Day, Alastair Kwok, Alison Evans, Fernanda Mata, Antonio Verdejo-Garcia, Kathryn Hart, Leigh C Ward, Helen Truby
This study aimed to compare the use of the bioelectrical impedance device (BIA) seca® mBCA 515 using dual X-ray absorptiometry (DXA) as a reference method, for body composition assessment in adults across the spectrum of body mass indices. It explores the utility of simple anthropometric measures (the waist height ratio (WHtR) and waist circumference (WC)) for the assessment of obesity. In the morning after an overnight fast (10 h), 30 participants underwent a body composition DXA (GE iDXA) scan, BIA (seca 515), and anthropometric measures...
October 10, 2018: Nutrients
Min Ji Yeon, Min Ho Lee, Do Hyun Kim, Ji Yeong Yang, Hyun Jun Woo, Hye Jin Kwon, Cheol Moon, Sa-Hyun Kim, Jong-Bae Kim
Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H...
October 5, 2018: Bioscience, Biotechnology, and Biochemistry
Priya Bariya, Linda L Randall
In all cells a highly conserved channel transports proteins across membranes. In E.coli , that channel is SecYEG. Many investigations of this protein complex have used purified SecYEG reconstituted into proteoliposomes. How faithfully do activities of reconstituted systems reflect the properties of SecYEG in the native membrane environment? We investigated by comparing three in vitro systems: the native membrane environment of inner membrane vesicles and two methods of reconstitution. One method was the widely used reconstitution of SecYEG alone into lipid bilayers...
October 1, 2018: Journal of Bacteriology
Isabel Ernst, Maximilian Haase, Stefan Ernst, Shuguang Yuan, Andreas Kuhn, Sebastian Leptihn
SecA is an essential molecular motor for the translocation of proteins across the membrane via the bacterial Sec secretion system. While the Sec system is found in all cells from archaea to multicellular eukaryotes, the SecA protein is mainly found in bacteria. The mechanism of how the motor protein works on a molecular level is still under dispute but it is well established that SecA binds ATP and uses its hydrolysis for the translocation of substrates. In this work, we addressed the question of which conformational changes the protein might undergo during protein translocation...
2018: Communications biology
Venkata Raveendra Pothineni, Mansi B Parekh, Mustafeez Mujtaba Babar, Aditya Ambati, Peter Maguire, Mohammed Inayathullah, Kwang-Min Kim, Lobat Tayebi, Hari-Hara Sk Potula, Jayakumar Rajadas
Background: Lyme disease accounts for >90% of all vector-borne disease cases in the United States and affect ~300,000 persons annually in North America. Though traditional tetracycline antibiotic therapy is generally prescribed for Lyme disease, still 10%-20% of patients treated with current antibiotic therapy still show lingering symptoms. Methods: In order to identify new drugs, we have evaluated four cephalosporins as a therapeutic alternative to commonly used antibiotics for the treatment of Lyme disease by using microdilution techniques like minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC)...
2018: Drug Design, Development and Therapy
Katherine González-Ruíz, María Medrano, Jorge Enrique Correa-Bautista, Antonio García-Hermoso, Daniel Humberto Prieto-Benavides, Alejandra Tordecilla-Sanders, César Agostinis-Sobrinho, María Correa-Rodríguez, Jacqueline Schmidt Rio-Valle, Emilio González-Jiménez, Robinson Ramírez-Vélez
Dual-energy X-ray absorptiometry (DXA) has been considered a reference method for measuring body fat percentage (BF%) in children and adolescents with an excess of adiposity. However, given that the DXA technique is impractical for routine field use, there is a need to investigate other methods that can accurately determine BF%. We studied the accuracy of bioelectrical impedance analysis (BIA) technology, including foot-to-foot and hand-to-foot impedance, and Slaughter skinfold-thickness equations in the measurement of BF%, compared with DXA, in a population of Latin American children and adolescents with an excess of adiposity...
August 14, 2018: Nutrients
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