keyword
Keywords milk fat globule epidermal gro...

milk fat globule epidermal growth factor Ⅷ

https://read.qxmd.com/read/36596780/mfg-e8-stabilized-by-deubiquitinase-usp14-suppresses-cigarette-smoke-induced-ferroptosis-in-bronchial-epithelial-cells
#21
JOURNAL ARTICLE
Yanan Cui, Lijuan Luo, Zihang Zeng, Xiangming Liu, Tiao Li, Xue He, Yiming Ma, Weiwei Meng, Huihui Zeng, Yingjiao Long, Dandan Zong, Yan Chen
Milk fat globule epidermal growth factor 8 (MFG-E8) participates in a range of cellular processes, including reducing apoptosis and oxidative stress. However, its protective activity against cigarette smoke-induced ferroptosis in the pathogenesis of the chronic obstructive pulmonary disease (COPD) and the modulation of MFG-E8 remain unclear. Here, we showed that cigarette smoke diminished MFG-E8 protein levels but had no significant effect on its mRNA levels in lung tissues of humans and mice and in two human bronchial epithelial cell lines...
January 3, 2023: Cell Death & Disease
https://read.qxmd.com/read/36484512/effects-of-mfg-e8-expression-on-the-biological-characteristics-of-ovarian-cancer-cells-via-the-akt-mtor-s6k-signalling-pathway
#22
JOURNAL ARTICLE
Na Li, Yazhuo Wang, Lin Liu, Pei Wang, Xiaohua Wu
In this study, we assessed the effects of MFG-E8 on the biological characteristics of ovarian cancer cells and explored the underlying mechanisms. Human ovarian cancer SKOV3 cells were transfected with MFG-E8 siRNA or NC siRNA. CCK-8, cell adhesion, scratch-wound, and Transwell assays were used to detect changes in cell metastatic processes. Effects of MFG-E8 silencing on the proteins involved in AKT/mTOR/S6K signalling pathway were assessed using qRT-PCR and Western blotting. Transient silencing of MFG-E8 in SKOV3 cells decreased cell proliferation and downregulated the expression of CDK4, cyclin D1, and caspase-3 proteins...
December 9, 2022: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://read.qxmd.com/read/36463380/mfg-e8-has-guiding-significance-for-the-prognosis-and-treatment-of-sepsis
#23
JOURNAL ARTICLE
Wei Wu, Jing Wang, Jingdi Chen, Jiaming Lu, Yaojia Lao, Kai Huang, Jun Lin
Sepsis remains a significant clinical challenge. Ferroptosis is involved in the pathogenesis of sepsis. Ferroptosis is associated with oxidative stress, and excessive oxidative stress is suppressed by milk fat globule epidermal growth factor 8 (MFG-E8) under various conditions. However, the role of MFG-E8 in sepsis-induced ferroptosis and oxidative stress is still unclear. First, we collected blood samples from patients with sepsis and detected the expression of serum MFG-E8. Then, the relationship between serum concentrations of MFG-E8 and disease severity was detected...
December 3, 2022: Scientific Reports
https://read.qxmd.com/read/36267465/elevated-mfg-e8-in-csf-in-the-early-stage-indicates-rapid-recovery-of-mild-aneurysmal-sah-patients
#24
JOURNAL ARTICLE
Cong Pang, Zheng Peng, Xiaojian Li, Yongyue Gao, Xunzhi Liu, Han Wang, Yue Lu, Zong Zhuang, Qingrong Zhang, Wei Li, Chunhua Hang
Background: Aneurysmal subarachnoid hemorrhage (aSAH) can impair blood perfusion in brain tissue and cause adverse effects. Microglia, which are the inherent immune cells of the brain, significantly activate and play a role in phagocytosis, anti-inflammatory, proinflammatory, and damage repair in this process. Milk fat globule epidermal growth factor 8 (MFG-E8) is the bridging molecule of this process and mediates the activation and biological effects of microglia. Methods: We obtained cerebrospinal fluid (CSF) from patients with aSAH at various times (the third day, seventh day, and ninth day) as well as from patients in the control cohort...
2022: Disease Markers
https://read.qxmd.com/read/36163411/preventing-sars-cov-2-infection-using-anti-spike-nanobody-ifn-%C3%AE-conjugated-exosomes
#25
JOURNAL ARTICLE
Xiabing Lyu, Shota Imai, Tomoyoshi Yamano, Rikinari Hanayama
PURPOSE: To inhibit the transmission of SARS-CoV-2, we developed engineered exosomes that were conjugated with anti-spike nanobodies and type I interferon β (IFN-β). We evaluated the efficacy and potency of nanobody-IFN-β conjugated exosomes to treatment of SARS-CoV-2 infection. METHODS: Milk fat globule epidermal growth factor 8 (MFG-E8) is a glycoprotein that binds to phosphatidylserine (PS) exposed on the exosomes. We generated nanobody-IFN-β conjugated exosomes by fusing an anti-spike nanobody and IFN-β with MFG-E8...
September 26, 2022: Pharmaceutical Research
https://read.qxmd.com/read/36000422/inflammatory-role-of-milk-fat-globule-epidermal-growth-factor-viii-in-age-associated-arterial-remodeling
#26
JOURNAL ARTICLE
Leng Ni, Lijuan Liu, Wanqu Zhu, Richard Telljohann, Jing Zhang, Robert E Monticone, Kimberly R McGraw, Changwei Liu, Christopher H Morrell, Pablo Garrido-Gil, Jose Luis Labandeira-Garcia, Edward G Lakatta, Mingyi Wang
Background Age-associated aortic remodeling includes a marked increase in intimal medial thickness (IMT), associated with signs of inflammation. Although aortic wall milk fat globule-epidermal growth factor VIII (MFG-E8) increases with age, and is associated with aortic inflammation, it is not known whether MFG-E8 is required for the age-associated increase in aortic IMT. Here, we tested whether MFG-E8 is required for the age-associated increase in aortic IMT. Methods and Results To determine the role of MFG-E8 in the age-associated increase of IMT, we compared aortic remodeling in adult (20-week) and aged (96-week) MFG-E8 (-/-) knockout and age matched wild-type (WT) littermate mice...
September 6, 2022: Journal of the American Heart Association
https://read.qxmd.com/read/35905868/sphingosine-1-phosphate-receptor-2-promotes-erythrocyte-clearance-by-vascular-smooth-muscle-cells-in-intraplaque-hemorrhage-through-mfg-e8-production
#27
JOURNAL ARTICLE
Daorong Pan, Wen Wu, Guangfeng Zuo, Xiangrong Xie, Hui Li, Xiaomin Ren, Chaohua Kong, Wenying Zhou, Zihan Zhang, Martin Waterfall, Shaoliang Chen
Intraplaque hemorrhage (IPH) accelerates atherosclerosis progression. To scavenge excessive red blood cells (RBCs), vascular smooth muscle cells (VSMCs) with great plasticity may function as phagocytes. Here, we investigated the erythrophagocytosis function of VSMCs and possible regulations involved. Based on transcriptional microarray analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that genes up-regulated in human carotid atheroma with IPH were enriched in functions of phagocytic activities, while those down-regulated were enriched in VSMCs contraction function...
July 26, 2022: Cellular Signalling
https://read.qxmd.com/read/35769208/mfg-e8-knockout-aggravated-nonalcoholic-steatohepatitis-by-promoting-the-activation-of-tlr4-nf-%C3%AE%C2%BA-b-signaling-in-mice
#28
JOURNAL ARTICLE
Jun Hu, Hui Du, Yinglin Yuan, Peipei Guo, Junxia Yang, Xinru Yin, Jin Liu, Shengwang Wu, Jingyuan Wan, Xia Gong
Nonalcoholic steatohepatitis (NASH) is the common liver disease characterized by hepatic steatosis, inflammation, and fibrosis; there are no approved drugs to treat this disease because of incomplete understanding of pathophysiological mechanisms of NASH. Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), a multifunctional glycoprotein, has shown anti-inflammation and antifibrosis. Here, MFG-E8 was shown to play a key role in NASH progression. Using methionine and choline deficient (MCD) diet-fed mice, we found MFG-E8 knockout exacerbated hepatic damage and steatosis as indicated by increased plasma transaminases activities and hepatic histopathologic change, higher hepatic triglycerides (TGs), and lipid accumulation...
2022: Mediators of Inflammation
https://read.qxmd.com/read/35681775/high-levels-of-mfg-e8-confer-a-good-prognosis-in-prostate-and-renal-cancer-patients
#29
JOURNAL ARTICLE
Karen Geoffroy, Patrick Laplante, Sylvie Clairefond, Feryel Azzi, Dominique Trudel, Jean-Baptiste Lattouf, John Stagg, Fred Saad, Anne-Marie Mes-Masson, Marie-Claude Bourgeois-Daigneault, Jean-François Cailhier
Milk fat globule-epidermal growth factor-8 (MFG-E8) is a glycoprotein secreted by different cell types, including apoptotic cells and activated macrophages. MFG-E8 is highly expressed in a variety of cancers and is classically associated with tumor growth and poor patient prognosis through reprogramming of macrophages into the pro-tumoral/pro-angiogenic M2 phenotype. To date, correlations between levels of MFG-E8 and patient survival in prostate and renal cancers remain unclear. Here, we quantified MFG-E8 and CD68/CD206 expression by immunofluorescence staining in tissue microarrays constructed from renal ( n = 190) and prostate ( n = 274) cancer patient specimens...
June 4, 2022: Cancers
https://read.qxmd.com/read/35597930/%C3%AE-v%C3%AE-3-targeted-sevs-for-efficient-intracellular-delivery-of-proteins-using-mfg-e8
#30
JOURNAL ARTICLE
Junxin Mai, Kai Wang, Chenxuexuan Liu, Sheng Xiong, Qiuling Xie
BACKGROUND: Small extracellular vesicles (sEVs) are nanometer-sized membranous particles shed by many types of cells and can transfer a multitude of cargos between cells. Recent studies of sEVs have been focusing on their potential to be novel drug carriers due to natural composition and other promising characteristics. However, there are challenges in sEVs-based drug delivery, one of which is the inefficient loading of drugs into sEVs, especially for large biomolecules. RESULTS: In this study, we proposed a membrane-associated protein, milk fat globule-epidermal growth factor 8 protein (MFG-E8), to produce αvβ3-targeted sEVs with high delivery efficiency of interested protein...
May 21, 2022: BMC Biotechnology
https://read.qxmd.com/read/35581508/milk-fat-globule-epidermal-growth-factor-8-a-potential-regulator-of-cutaneous-wound-healing
#31
REVIEW
Yun Xiao, Ying Tang, Xiao Shu, Jinshuo Zhu, Xinan Wu, Yuan Zhang
Destroying the integrity of the skin may causes disability and even death from injury or illness. Wound healing is a core mechanism to maintain skin barrier function. Milk fat-globule epidermal growth factor 8 (MFG-E8) is a key factor in wound healing and is involved in regulating blood coagulation, mediating macrophage uptake of apoptotic cells, shifting macrophages from an inflammatory to an anti-inflammatory phenotype, promoting angiogenesis, enhancing vascular endothelial growth factor (VEGF) signaling, and assisting wound tissue perfusion...
May 17, 2022: Molecular Biology Reports
https://read.qxmd.com/read/35523889/invariant-nkt-cell-augmented-gm-csf-secreting-tumor-vaccine-is-effective-in-advanced-prostate-cancer-model
#32
JOURNAL ARTICLE
Bindu Varghese, Lydia Lynch, Lianne E Vriend, Dobrin Draganov, Justice M Clark, Haydn T Kissick, Sharlin Varghese, Martin G Sanda, Glenn Dranoff, M Simo Arredouani, Steven P Balk, Mark A Exley
Invariant natural killer T cells (iNKT cells) express a semi-invariant T cell receptor that recognizes certain glycolipids (including α-galactosylceramide, αGC) bound to CD1d, and can induce potent antitumor responses. Here, we assessed whether αGC could enhance the efficacy of a GM-CSF-producing tumor cell vaccine in the transgenic SV40 T antigen-driven TRAMP prostate cancer model. In healthy mice, we initially found that optimal T cell responses were obtained with αGC-pulsed TRAMP-C2 cells secreting GM-CSF and milk fat globule epidermal growth factor protein-8 (MFG-E8) with an RGD to RGE mutation (GM-CSF/RGE TRAMP-C2), combined with systemic low dose IL-12...
December 2022: Cancer Immunology, Immunotherapy: CII
https://read.qxmd.com/read/35503264/mfge8-decreased-neuronal-apoptosis-and-neuroinflammation-to-ameliorate-early-brain-injury-induced-by-subarachnoid-hemorrhage-through-the-inhibition-of-hmgb1
#33
JOURNAL ARTICLE
Jiaoxue Qiu, Wenna Li, Rutao Mu, Lingling Wang, Lei Guo, Lili Ma
AIM: Both MFGE8 and HMGB1 were vital players for aneurysmal subarachnoid hemorrhage. However, whether HMGB1 was served as the downstream target of MFGE8 was unknown. To test this new mechanism, we performed the SAH model in rats. METHOD: All treatments were injected intraventricularly into the right lateral ventricles. SAH grade, brain water content, and neurological function scores were evaluated. HMGB1 expression was studied by double immunofluorescence staining...
January 2022: Human & Experimental Toxicology
https://read.qxmd.com/read/35456911/mfg-e8-reduces-aortic-intimal-proliferation-in-a-murine-model-of-transplant-vasculopathy
#34
JOURNAL ARTICLE
Benoit Brilland, Patrick Laplante, Pamela Thebault, Karen Geoffroy, Marie-Joëlle Brissette, Mathieu Latour, Michaël Chassé, Shijie Qi, Marie-Josée Hébert, Héloïse Cardinal, Jean-François Cailhier
Transplant vasculopathy is characterized by endothelial apoptosis, which modulates the local microenvironment. Milk fat globule epidermal growth factor 8 (MFG-E8), which is released by apoptotic endothelial cells, limits tissue damage and inflammation by promoting anti-inflammatory macrophages. We aimed to study its role in transplant vasculopathy using the murine aortic allotransplantation model. BALB/c mice were transplanted with fully mismatched aortic transplants from MFG-E8 knockout (KO) or wild type (WT) C57BL/6J mice...
April 7, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/35456012/regenerating-skeletal-muscle-compensates-for-the-impaired-macrophage-functions-leading-to-normal-muscle-repair-in-retinol-saturase-null-mice
#35
JOURNAL ARTICLE
Nastaran Tarban, Hajnalka Halász, Péter Gogolák, Éva Garabuczi, Alexander R Moise, Krzysztof Palczewski, Zsolt Sarang, Zsuzsa Szondy
Skeletal muscle repair is initiated by local inflammation and involves the engulfment of dead cells (efferocytosis) by infiltrating macrophages at the injury site. Macrophages orchestrate the whole repair program, and efferocytosis is a key event not only for cell clearance but also for triggering the timed polarization of the inflammatory phenotype of macrophages into the healing one. While pro-inflammatory cytokines produced by the inflammatory macrophages induce satellite cell proliferation and differentiation into myoblasts, healing macrophages initiate the resolution of inflammation, angiogenesis, and extracellular matrix formation and drive myoblast fusion and myotube growth...
April 13, 2022: Cells
https://read.qxmd.com/read/35440618/mfg-e8-promotes-osteogenic-transdifferentiation-of-smooth-muscle-cells-and-vascular-calcification-by-regulating-tgf-%C3%AE-1-signaling
#36
JOURNAL ARTICLE
Hou-Yu Chiang, Pao-Hsien Chu, Shao-Chi Chen, Ting-Hein Lee
Vascular calcification occurs in arterial aging, atherosclerosis, diabetes mellitus, and chronic kidney disease. Transforming growth factor-β1 (TGF-β1) is a key modulator driving the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs), leading to vascular calcification. We hypothesize that milk fat globule-epidermal growth factor 8 (MFG-E8), a glycoprotein expressed in VSMCs, promotes the osteogenic transdifferentiation of VSMCs through the activation of TGF-β1-mediated signaling. We observe that the genetic deletion of MFG-E8 prevents calcium chloride-induced vascular calcification in common carotid arteries (CCAs)...
April 19, 2022: Communications Biology
https://read.qxmd.com/read/34953020/increased-mfg-e8-at-neuromuscular-junctions-is-an-exacerbating-factor-for-sarcopenia-associated-denervation
#37
JOURNAL ARTICLE
Madoka Ikemoto-Uezumi, Heying Zhou, Tamaki Kurosawa, Yuki Yoshimoto, Masashi Toyoda, Nobuo Kanazawa, Tatsu Nakazawa, Mitsuhiro Morita, Kunihiro Tsuchida, Akiyoshi Uezumi
Sarcopenia is an important health problem associated with adverse outcomes. Although the etiology of sarcopenia remains poorly understood, factors apart from muscle fibers, including humoral factors, might be involved. Here, we used cytokine antibody arrays to identify humoral factors involved in sarcopenia and found a significant increase in levels of milk fat globule epidermal growth factor 8 (MFG-E8) in skeletal muscle of aged mice, compared with young mice. We found that the increase in MFG-E8 protein at arterial walls and neuromuscular junctions (NMJs) in muscles of aged mice...
January 2022: Aging Cell
https://read.qxmd.com/read/34928287/identification-of-mitochondria-related-hub-genes-in-sarcopenia-and-functional-regulation-of-mfg-e8-on-ros-mediated-mitochondrial-dysfunction-and-cell-cycle-arrest
#38
JOURNAL ARTICLE
He Li, Kaifang Guan, DanDan Liu, Min Liu
Sarcopenia has high prevalence in the elderly population, but the genes and pathways related to aging in elderly patients with sarcopenia are poorly understood. Milk fat globule epidermal growth factor 8 (MFG-E8) is a peripheral membrane glycoprotein isolated from the milk fat globule membrane (MFGM). It has been found to exhibit various nutritional effects, including antibacterial, anti-cancer, anti-oxidant, anti-sarcopenia, and improving brain development and cognitive effects. This study aimed to investigate key differentially expressed genes (DEGs) and pathways associated with the progression of sarcopenia using bioinformatics analysis and in vitro myoblast experiment...
January 24, 2022: Food & Function
https://read.qxmd.com/read/34685562/cx3cl1-microparticles-induced-mfg-e8-enhances-apoptotic-cell-clearance-by-alveolar-macrophages
#39
JOURNAL ARTICLE
Wen-Hui Tsai, Shao-Chi Chang, Yu-Chieh Lin, Hui-Chi Hsu
During the resolution phase of acute lung injury, apoptotic cells release CX3CL1 as a "find-me" signal to attract alveolar macrophage transmigration toward apoptotic cells for phagocytosis. However, it is still not clear whether CX3CL1 has pro-phagocytic activity on alveolar macrophage. In this study, we investigated the role of apoptotic NB4 cells-derived CX3CL1(+) microparticles (apo-MP) on the phagocytic activity of NR8383 cells. We demonstrate that exogenous CX3CL1 and apo-MP enhanced the phagocytic activity of NR8383 cells in a CX3 CR1-dependent manner...
September 28, 2021: Cells
https://read.qxmd.com/read/34681627/extracellular-vesicles-from-human-teeth-stem-cells-trigger-atp-release-and-promote-migration-of-human-microglia-through-p2x4-receptor-mfg-e8-dependent-mechanisms
#40
JOURNAL ARTICLE
Ugnė Jonavičė, Diana Romenskaja, Karolina Kriaučiūnaitė, Akvilė Jarmalavičiūtė, Justina Pajarskienė, Vytautas Kašėta, Virginijus Tunaitis, Tarja Malm, Rashid Giniatulin, Augustas Pivoriūnas
Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate the neuroinflammatory response of microglia remains largely unexplored. Here, we addressed this issue by testing the action of EVs derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized human microglial cells. We found that EVs induced a rapid increase in intracellular Ca2+ and promoted significant ATP release in microglial cells after 20 min of treatment...
October 11, 2021: International Journal of Molecular Sciences
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