Brianna Steiert, Shelby E Andersen, Paige N McCaslin, Cherilyn A Elwell, Robert Faris, Xavier Tijerina, Parker Smith, Quinn Eldridge, Brian S Imai, Justine V Arrington, Peter M Yau, Kathleen M Mirrashidi, Jeffrey R Johnson, Erik Verschueren, John Von Dollen, Gwendolyn M Jang, Nevan J Krogan, Joanne N Engel, Mary M Weber
Chlamydia trachomatis ( C.t .), the leading cause of bacterial sexually transmitted infections, employs a type III secretion system (T3SS) to translocate two classes of effectors, inclusion membrane proteins and conventional T3SS (cT3SS) effectors, into the host cell to counter host defense mechanisms. Here we employed three assays to directly evaluate secretion during infection, validating secretion for 23 cT3SS effectors. As bioinformatic analyses have been largely unrevealing, we conducted affinity purification-mass spectrometry to identify host targets and gain insights into the functions of these effectors, identifying high confidence interacting partners for 21 cT3SS effectors...
April 25, 2024: bioRxiv