Yaoqiang Shi, Qi Tan, Tao Gong, Qing-Yuan Li, Ya Zhu, Xiaoqiong Duan, Chunhui Yang, Jia-Wei Ding, Shilin Li, He Xie, Yujia Li, Limin Chen
Pathogen infections including Shigella flexneri have posed a significant threat to human health for numerous years. Although culturing and qPCR were the gold standards for pathogen detection, time-consuming and instrument-dependent restrict their application in rapid diagnosis and economically less-developed regions. Thus, it is urgently needed to develop rapid, simple, sensitive, accurate, and low-cost detection methods for pathogen detection. In this study, an immunomagnetic beads-recombinase polymerase amplification-CRISPR/Cas12a (IMB-RPA-CRISPR/Cas12a) method was built based on a cascaded signal amplification strategy for ultra-specific, ultra-sensitive, and visual detection of S...
April 17, 2024: Mikrochimica Acta