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https://read.qxmd.com/read/30779780/novel-polyclonal-antibody-based-rapid-gold-sandwich-immunochromatographic-strip-for-detecting-the-major-royal-jelly-protein-1-mrjp1-in-honey
#1
Yifan Zhang, Yong Chen, Yiting Cai, Zongyan Cui, Jinjie Zhang, Xiaohou Wang, Lirong Shen
Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal...
2019: PloS One
https://read.qxmd.com/read/30778744/prokaryotic-expression-purification-and-evaluation-of-goatpox-virus-orf117-protein-as-a-diagnostic-antigen-in-indirect-elisa-to-detect-goatpox
#2
M Dashprakash, G Venkatesan, Amit Kumar, M Sankar, Sargam Arya, M A Ramakrishnan, A B Pandey, B Mondal
Goatpox is an economically significant transboundary viral disease of goats that is caused by goatpox virus (GTPV). This study describes the prokaryotic expression of the GTPV ORF117 protein, a homologue of vaccinia virus A27L, and evaluation of its diagnostic potential in ELISA. The GTPV ORF117 gene was cloned into the pET32a vector to express recombinant ORF117 protein (rA27L) in E. coli BL21-CodonPlus (DE3)-RIPL. The bacterial expression of the protein was confirmed by western blot analysis using anti-GTPV polyclonal antibodies that detected rA27L, which is ~ 35 kDa in size...
February 18, 2019: Archives of Virology
https://read.qxmd.com/read/30778384/peptide-functionalization-of-gold-nanoparticles-for-the-detection-of-carcinoembryonic-antigen-in-blood-plasma-via-spr-based-biosensor
#3
Maria Laura Ermini, Xue Chadtová Song, Tomáš Špringer, Jiří Homola
Nanoparticles functionalized with specific biological recognition molecules play a major role for sensor response enhancement in surface plasmon resonance (SPR) based biosensors. The functionalization procedure of such nanoparticles is crucial, since it influences their interactions with the environment and determines their applicability to biomolecular detection in complex matrices. In this work we show how the ζ-potential (Zpot) of bio-functionalized gold spherical NPs (Bio-NPs) is related to the SPR sensor response enhancement of an immune-sandwich-assay for the detection of the carcinoembryonic antigen (CEA), a cancer marker for colorectal carcinomas...
2019: Frontiers in Chemistry
https://read.qxmd.com/read/30776575/performance-evaluation-of-the-hologic-aptima-hcv-quant-dx-assay-for-detection-of-hcv-rna-from-dried-blood-spots
#4
Beth Catlett, Alex Carrera, Mitchell Starr, Tanya L Applegate, Peter Lowe, H Philip Cunningham, Jason Grebely
BACKGROUND: The availability of effective direct-acting antiviral therapy for hepatitis C virus (HCV) has led to a need for simplified diagnostic pathways. Barriers to treatment uptake, specifically in people who inject drugs and in remote and resource limited settings, may be overcome by utilizing novel collection methods, such as dried blood spots (DBS). However, there are currently no registered assays for HCV RNA testing from DBS samples. OBJECTIVES: To evaluate the sensitivity and specificity of the Aptima HCV Dx Quant assay for HCV RNA detection in DBS samples STUDY DESIGN: 107 paired venepuncture and DBS samples from HCV antibody positive individuals were analyzed for HCV RNA on the Aptima HCV Dx Quant and Roche CAP/CTM (gold standard) HCV assays...
January 25, 2019: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://read.qxmd.com/read/30776321/the-use-of-erpy-like-recombinant-protein-from-leptospira-interrogans-in-the-development-of-an-immunodiagnostic-test-for-swine-leptospirosis
#5
Bárbara Couto Roloff Padilha, Henrique Queiroz Simão, Thaís Larré Oliveira, Daiane Drawanz Hartwig
Swine leptospirosis poses a major problem in the agricultural sector. The gold standard for serodiagnosis of leptospirosis is Microscopic Agglutination Test (MAT). However, the limitations of this technique make the search for alternative diagnostic methods inevitable. In the present study, ErpY-like recombinant protein (rErpY-like), produced in Escherichia coli and used as antigen in indirect enzyme-linked immunosorbent assay (ELISA), was evaluated for its efficacy as a novel diagnostic tool for swine leptospirosis...
February 15, 2019: Acta Tropica
https://read.qxmd.com/read/30776214/microencapsulated-immunoassays-for-detection-of-cytokines-in-human-blood
#6
Ali Rahimian, Christian Siltanen, Hamid Feyzizarnagh, Patricio Escalante, Alexander Revzin
Cytokines are produced by leukocytes in blood and may be used as indicators of malignancies or infections. The objective of this study was to develop a strategy for immunosensing cytokines in whole, unprocessed human blood. Microfluidic droplet generation was employed to fabricate ∼400 μm diameter microcapsules with a hydrogel shell and an aqueous core containing sensing microbeads. The hydrogel shell was composed of poly(ethylene glycol) forming a thin (∼10 μm) immunoisolation layer protecting antibody-modified microbeads inside the capsule from immune cells on the outside...
February 18, 2019: ACS Sensors
https://read.qxmd.com/read/30774993/three-cell-deaths-and-a-funeral-macrophage-clearance-of-cells-undergoing-distinct-modes-of-cell-death
#7
Katharina Klöditz, Bengt Fadeel
Macrophage clearance of apoptotic cells has been extensively investigated, but less is known regarding the clearance of cells dying by other forms of programmed cell death, e.g., necroptosis or ferroptosis. Here, we established a model of three different cell deaths using the same cell line and the occurrence of distinct cell death modalities was verified by using the specific inhibitors, zVAD-fmk, necrostatin-1, and ferrostatin-1, respectively. Cell death was characterized by using transmission electron microscopy (TEM), the gold standard for the demarcation of different cell death modalities...
2019: Cell Death Discovery
https://read.qxmd.com/read/30771960/an-fluorescent-aptasensor-for-sensitive-detection-of-tumor-marker-based-on-the-fret-of-a-sandwich-structured-qds-afp-aunps
#8
Lu Zhou, Feihong Ji, Tao Zhang, Feng Wang, Yuchuan Li, Zhixin Yu, Xiaoping Jin, Bing Ruan
The detection of alpha-fetoprotein (AFP) is of great importance for hepatocellular carcinoma (HCC) diagnosis, but it needs to be further improved because of poor sensitivity and complicated operating steps. In this paper, a simple and sensitive homogeneous apatasensor for AFP has been developed based on Förster resonance energy transfer (FRET) where the AFP aptamer labeled luminescent CdTe quantum dots (QDs) as a donor and anti-AFP antibody functional gold nanoparticles (AuNPs) as an acceptor. In the presence of AFP, the bio-affinity between aptamer, target, and antibody made the QDs and AuNPs close enough, thus the fluorescence of CdTe QDs quenched though the FRET between QD and AuNP...
May 15, 2019: Talanta
https://read.qxmd.com/read/30771955/laser-ablation-icp-ms-for-simultaneous-quantitative-imaging-of-iron-and-ferroportin-in-hippocampus-of-human-brain-tissues-with-alzheimer-s-disease
#9
María Cruz-Alonso, Beatriz Fernandez, Ana Navarro, Susana Junceda, Aurora Astudillo, Rosario Pereiro
Laser ablation inductively coupled plasma - mass spectrometry (LA-ICP-MS) is proposed for a better understanding of metals and proteins distribution in micrometre structures of human brain tissues. Simultaneous absolute quantitative imaging of Fe and ferroportin (FPN), in 5 µm thick tissue sections of the stratum pyramidale of hippocampus CA1 region, was carried out for Alzheimer disease (AD) patients and healthy controls (HC). For the imaging of FPN by LA-ICP-MS, antibodies were labelled via carbodiimide crosslinking with fluorescent gold nanoclusters (AuNCs) of 2...
May 15, 2019: Talanta
https://read.qxmd.com/read/30764933/immunosensor-for-the-diagnostics-of-autoimmune-hemolytic-anemia-aiha-based-on-immobilization-of-a-monoclonal-antibody-on-a-layer-of-silk-fibroin
#10
Marli L Moraes, Lais R Lima, Josy C Vicentini-Oliveira, Aparecida Vitória G de Souza, Osvaldo N Oliveira, Elenice Deffune, Sidney J L Ribeiro
The diagnostics of the autoimmune hemolytic anemia (AIHA), a rare disease caused by autoantibody-induced hemolysis, is still prone to false positives for it is based on visual observation in the so-called Direct Coombs test. In this study, we developed a specific IgG hemolysis immunosensor produced with layer-by-layer (LbL) films containing a monoclonal antibody against human immunoglobulin (mAbIMUG) deposited along with a layer of silk fibroin (SF) derived from Bombyx mori cocoons. Adsorption of mAbIMUG on a SF layer was confirmed by the fluorescence emission band at 326 nm...
July 1, 2019: Journal of Nanoscience and Nanotechnology
https://read.qxmd.com/read/30760184/development-of-a-gold-nanoparticle-labeled-sandwich-format-lateral-flow-immunoassay-kit-for-the-detection-of-tropical-house-dust-mite-suidasia-pontifica
#11
Mark Angelo V Ngu, Jose H Bergantin, John Donnie A Ramos
The house dust mite Suidasia pontifica (Sp) is an important source of allergens in tropical regions that trigger IgE-mediated allergic reactions such as allergic asthma, atopic dermatitis and allergic rhinitis. Detection of Sp-specific proteins are important in the management and prevention of allergic diseases. The study aimed to provide a proof of concept for a gold nanoparticle-labeled sandwich format lateral flow immunoassay (LFIA) kit for the detection of Sp-specific proteins. Protein A chromatography-purified rabbit anti-Sp polyclonal antibodies were labeled with gold nanoparticles (AuNP) synthesized from chloroauric acid using the citrate reduction method, then dispensed on a glass fiber pad...
February 12, 2019: Protein and Peptide Letters
https://read.qxmd.com/read/30758174/facile-synthesis-of-cu2o-tio2-ptcu-nanocomposites-as-signal-amplification-strategy-for-the-insulin-detection
#12
Faying Li, Jinhui Feng, Zengqiang Gao, Li Shi, Dan Wu, Bin Du, Qin Wei
A novel ultrasensitive sandwich-type electrochemical immunosensor was proposed for the quantitative detection of insulin, a representative biomarker for diabetes. To this end, molybdenum disulfide nanosheets loaded gold nanoparticles (MoS2/Au NPs) were used as substrates to modify bare glassy carbon electrodes (GCE). The MoS2/Au NPs not only present superior biocompatible and large specific surface area to enhance the loading capacity of primary antibody (Ab1), but also present good electrical conductivity to accelerate electron transfer rate...
February 13, 2019: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/30744018/ultrasensitive-immunosensor-array-for-tnf-%C3%AE-detection-in-artificial-saliva-using-polymer-coated-magnetic-microparticles-onto-screen-printed-gold-electrode
#13
Lassaad Barhoumi, Francesca G Bellagambi, Federico M Vivaldi, Abdoullatif Baraket, Yohann Clément, Nadia Zine, Mounir Ben Ali, Abdelhamid Elaissari, Abdelhamid Errachid
Tumor necrosis factor-α (TNF-α) is a biomarker of inflammation that occurs in patients suffering from heart failure (HF). Saliva can be sampled in a non-invasive way, and it is currently gaining importance as matrix alternative to blood in diagnostic and therapy monitoring. This work presents the development of an immunosensor array based on eight screen-printed gold electrodes to detect TNF-α in saliva samples. Two different functionalization strategies of electrodes were compared. In the first, anti-TNF-α antibodies were chemically bonded onto the electrode by functionalization with 4-carboxymethylaniline...
February 8, 2019: Sensors
https://read.qxmd.com/read/30740097/evaluation-of-a-novel-immunoassay-for-quantification-of-c1q-for-clinical-diagnostic-use
#14
Kerstin Sandholm, Barbro Persson, Lillemor Skattum, Gösta Eggertsen, Dag Nyman, Iva Gunnarsson, Elisabet Svenungson, Bo Nilsson, Kristina N Ekdahl
Objectives: C1q is a valuable biomarker of disease activity in systemic lupus erythematosus (SLE). The "gold standard" assay, rocket immunoelectrophoresis (RIE), is time-consuming, and thus a shift to soluble immune precipitation techniques such as nephelometry has occurred. However, quantification of C1q with these techniques has been questioned as a result of the antibody binding properties of C1q. In the present work, we have compared results using various techniques (RIE, nephelometry, and ELISA) and have developed and validated a new magnetic bead-based sandwich immunoassay (MBSI)...
2019: Frontiers in Immunology
https://read.qxmd.com/read/30735354/avoiding-pre-isolation-step-in-exosome-analysis-direct-isolation-and-sensitive-detection-of-exosomes-using-gold-loaded-nanoporous-ferric-oxide-nanozymes
#15
Kseniia Boriachek, Mostafa Kamal Masud, Carlos Palma, Hoang-Phuong Phan, Yusuke Yamauchi, Md Shahriar A Hossain, Nam-Trung Nguyen, Carlos Salomon, Muhammad J A Shiddiky
Most of the current strategies for exosome analysis are time-consuming and largely dependent on pre-isolation step using a commercial extraction kit, which requires extensive sample manipulation, costly isolation, reagents, tedious procedures, and sophisticated equipment, and is prone to bias/artefacts. Herein we introduce a simple method for direct isolation and subsequent detection of a specific population of exosomes using an engineered superparamagnetic material with multi-functional properties, namely gold-loaded ferric oxide nanocubes (Au-NPFe2O3NC)...
February 8, 2019: Analytical Chemistry
https://read.qxmd.com/read/30735189/quantification-of-antibody-dependent-enhancement-of-the-zika-virus-in-primary-human-cells
#16
Sultan Asad, Fabiana Feitosa-Suntheimer, Alexander Gold, Berlin Londono-Renteria, Tonya M Colpitts
The recent emergence of the flavivirus Zika and neurological complications, such as Guillain-Barré syndrome and microcephaly in infants, has brought serious public safety concerns. Among the risk factors, antibody-dependent enhancement (ADE) poses the most significant threat, as the recent re-emergence of the Zika virus (ZIKV) is primarily in areas where the population has been exposed and is in a state of pre-immunity to other closely related flaviviruses, especially dengue virus (DENV). Here, we describe a protocol for quantifying the effect of human serum antibodies against DENV on ZIKV infection in primary human cells or cell lines...
January 18, 2019: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/30722881/ultrasensitive-detection-of-staphylococcal-enterotoxin-b-in-foodstuff-through-dual-signal-amplification-by-bio-barcode-and-real-time-pcr
#17
Yanyang Xu, Bingyang Huo, Chao Li, Yuan Peng, Sai Tian, Longxing Fan, Jialei Bai, Baoan Ning, Zhixian Gao
This study developed an ultrasensitive method involving (1) gold nanoparticles encoded with a double-stranded DNA as the first signal amplification and goat anti-staphylococcal enterotoxin B (SEB) polyclonal antibody and (2) magnetic microparticles coated with anti-SEB monoclonal antibody to detect SEB. Both functionalized nanoparticles can capture SEB in a sandwich system. The released DNA barcodes were then characterized through SYBR Green real-time polymerase chain reaction and resulted in the second signal amplification...
June 15, 2019: Food Chemistry
https://read.qxmd.com/read/30718327/author-response-video-neuroimages-paraneoplastic-spinal-myoclonus-associated-with-caspr2-antibodies
#18
Harrison Hines, Nick M Murray, Sarah Ahmad, Safwan Jaradeh, Carl A Gold
No abstract text is available yet for this article.
February 5, 2019: Neurology
https://read.qxmd.com/read/30717341/long-range-surface-plasmon-polariton-waveguide-biosensors-for-human-cardiac-troponin-i-detection
#19
Oleksiy Krupin, Pierre Berini
Straight long-range surface plasmon-polariton (LRSPP) waveguides as biosensors for label-free detection are discussed. The sensors consist of 5-μm-wide 35-nm-thick gold stripes embedded in a low-index optical-grade fluoropolymer (CYTOPTM ) with fluidic channels etched to the Au surface of the stripes. This work demonstrates the application of the LRSPP biosensors for the detection of human cardiac troponin I (cTnI) protein. cTnI is a biological marker for acute myocardial infarction (AMI), often referred to as a heart attack, which can be diagnosed by elevated levels of cTnI in patient blood...
February 2, 2019: Sensors
https://read.qxmd.com/read/30716591/gold-nanorod-enhances-dielectric-voltammetry-detection-of-c-reactive-protein-a-predictive-strategy-for-cardiac-failure
#20
Iswary Letchumanan, M K Md Arshad, S R Balakrishnan, Subash C B Gopinath
This paper primarily demonstrates the approach to enhance the sensing performance on antigen C-reactive protein (CRP) and anti-CRP antibody binding event. A nanogapped electrode structure with the gap of ~100 nm was modified by the anti-CRP antibody (Probe) to capture the available CRP. In order to increase the amount of antigen to be captured, a gold nanorod with 119 nm in length and 25 nm in width was integrated, to increase the surface area. A comparative study between the existence and non-existence of gold nanorod utilization was evaluated...
January 29, 2019: Biosensors & Bioelectronics
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