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STC-1 cell fatty acids

David Planes-Muñoz, Rubén López-Nicolás, Carlos A González-Bermúdez, Gaspar Ros-Berruezo, Carmen Frontela-Saseta
Plant extract activity can be modified by the digestion process. In order to assess the satiety effect of green tea and turmeric extracts, an in vitro gastrointestinal digestion process was performed and the STC-1 cell line was used. The enteroendocrine cell line was incubated (for 30, 60 and 120 minutes) with the digested and non-digested extracts measuring the secretion of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) by enzyme-linked immunosorbent assay (ELISA). The release of satiety hormones by the STC-1 cells showed similar or higher results for plant extracts compared to the positive controls reported as satiatogenic (proteins and short chain fatty acids)...
October 17, 2018: Food & Function
Tohru Hira, Shono Ogasawara, Asuka Yahagi, Minami Kamachi, Jiaxin Li, Saki Nishimura, Masayoshi Sakaino, Takatoshi Yamashita, Shigenobu Kishino, Jun Ogawa, Hiroshi Hara
SCOPE: The secretion of gut hormones, such as cholecystokinin (CCK) is stimulated by fatty acids. Although a chain length-dependent mechanism has been proposed, other structural relationships to releasing activity remain unclear. We aimed to elucidate specific structures in fatty acids that are responsible for their CCK-releasing activity, and related sensing mechanisms in enteroendocrine cells. METHODS AND RESULTS: CCK secretory activities were examined in a murine CCK-producing cell line STC-1 by exposing the cells to various modified fatty acids produced by gut lactic acid bacteria...
October 2018: Molecular Nutrition & Food Research
L Shcherbina, A Lindqvist, A-H Thorén Fischer, E Ahlqvist, E Zhang, S E Falkmer, E Renström, J Koffert, H Honka, N Wierup
Impaired incretin effect is a culprit in Type 2 Diabetes. Cocaine- and amphetamine-regulated transcript (CART) is a regulatory peptide controlling pancreatic islet hormone secretion and beta-cell survival. Here we studied the potential expression of CART in enteroendocrine cells and examined the role of CART as a regulator of incretin secretion and expression. CART expression was found in glucose-dependent insulinotropic polypeptide (GIP)-producing K-cells and glucagon-like peptide-1 (GLP-1)-producing L-cells in human duodenum and jejunum and circulating CART levels were increased 60 min after a meal in humans...
November 15, 2018: Molecular and Cellular Endocrinology
Shunsuke Yamane, Norio Harada, Nobuya Inagaki
Gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide (GIP) is one of the incretins, which are gastrointestinal hormones released in response to nutrient ingestion and potentiate glucose-stimulated insulin secretion. Single fat ingestion stimulates GIP secretion from enteroendocrine K cells; chronic high-fat diet (HFD) loading enhances GIP secretion and induces obesity in mice in a GIP-dependent manner. However, the mechanisms of GIP secretion from K cells in response to fat ingestion and GIP hypersecretion in HFD-induced obesity are not well understood...
April 2016: Journal of Diabetes Investigation
Ai-Jun Li, Qing Wang, Thu T Dinh, Steve M Simasko, Sue Ritter
Mercaptoacetate (MA) is an orexigenic agent reported to block fatty acid (FA) oxidation. Recently, however, we reported evidence from isolated nodose ganglion neurons that MA antagonizes the G protein-coupled long- and medium-chain FA receptor GPR40. GPR40 mediates FA-induced secretion of the satietogenic incretin peptide glucagon-like peptide 1 (GLP-1), by enteroendocrine L cells, as well as FA-induced enhancement of glucose-stimulated insulin secretion. Our results in cultured nodose neurons suggest that MA would also block GPR40 in enteroendocrine cells controlling GLP-1 secretion...
April 15, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Arivarasu N Anbazhagan, Shubha Priyamvada, Tarunmeet Gujral, Sumit Bhattacharyya, Waddah A Alrefai, Pradeep K Dudeja, Alip Borthakur
GPR120 (free fatty acid receptor-4) is a G protein-coupled receptor for medium- and long-chain unsaturated fatty acids, including ω-3 fatty acids. Recent studies have shown GPR120 to play cardinal roles in metabolic disorders via modulation of gut hormone secretion and insulin sensitivity and to exert anti-inflammatory effects in macrophages and adipose tissues. However, information on anti-inflammatory role of GPR120 at the level of intestinal epithelium is very limited. Current studies demonstrated differential levels of GPR120 mRNA and protein along the length of the human, mouse, and rat intestine and delineated distinct anti-inflammatory responses following GPR120 activation in model human intestinal epithelial Caco-2 cells, but not in model mouse intestinal epithelial endocrine cell line STC-1...
April 1, 2016: American Journal of Physiology. Cell Physiology
Alastair Khodabukus, Keith Baar
Satellite cells derived from fast and slow muscles have been shown to adopt contractile and metabolic properties of their parent muscle. Mouse muscle shows less distinctive fiber-type profiles than rat or rabbit muscle. Therefore, in this study we sought to determine whether three-dimensional muscle constructs engineered from slow soleus (SOL) and fast tibialis anterior (TA) from mice would adopt the contractile and metabolic properties of their parent muscle. Time-to-peak tension (TPT) and half-relaxation time (1/2RT) was significantly slower in SOL constructs...
August 2015: Journal of Cellular Physiology
Mehmet Hakan Ozdener, Selvakumar Subramaniam, Sinju Sundaresan, Omar Sery, Toshihiro Hashimoto, Yoshinori Asakawa, Philippe Besnard, Nada A Abumrad, Naim Akhtar Khan
BACKGROUND & AIMS: It is important to increase our understanding of gustatory detection of dietary fat and its contribution to fat preference. We studied the roles of the fat taste receptors CD36 and GPR120 and their interactions via Ca(2+) signaling in fungiform taste bud cells (TBC). METHODS: We measured Ca(2+) signaling in human TBC, transfected with small interfering RNAs against messenger RNAs encoding CD36 and GPR120 (or control small interfering RNAs). We also studied Ca(2+) signaling in TBC from CD36(-/-) mice and from wild-type lean and obese mice...
April 2014: Gastroenterology
Shingo Nakajima, Tohru Hira, Asuka Yahagi, Chigusa Nishiyama, Takatoshi Yamashita, Jun Imagi, Hiroshi Hara
SCOPE: Cholecystokinin (CCK) producing cells sense luminal contents to regulate the exocrine pancreas, gastric motility, and appetite. Although long-chain fatty acids (FAs, ≥ C12) are well known to stimulate CCK secretion, the CCK-releasing activities of other aliphatic compounds, such as aldehydes (Alds) or alcohols (Alcs), have not been studied. METHODS AND RESULTS: We tested the CCK-releasing activities of various aliphatic compounds with various carbon chain lengths (C3-C13) and degrees of unsaturation in the enteroendocrine cell line STC-1...
May 2014: Molecular Nutrition & Food Research
Brian D Hudson, Maria E Due-Hansen, Elisabeth Christiansen, Anna Mette Hansen, Amanda E Mackenzie, Hannah Murdoch, Sunil K Pandey, Richard J Ward, Rudi Marquez, Irina G Tikhonova, Trond Ulven, Graeme Milligan
FFA2 is a G protein-coupled receptor that responds to short chain fatty acids and has generated interest as a therapeutic target for metabolic and inflammatory conditions. However, definition of its functions has been slowed by a dearth of selective ligands that can distinguish it from the closely related FFA3. At present, the only selective ligands described for FFA2 suffer from poor potency, altered signaling due to allosteric modes of action, or a lack of function at non-human orthologs of the receptor. To address the need for novel selective ligands, we synthesized two compounds potentially having FFA2 activity and examined the molecular basis of their function...
June 14, 2013: Journal of Biological Chemistry
Sinju Sundaresan, Rafiq Shahid, Terrence E Riehl, Rashmi Chandra, Fatiha Nassir, William F Stenson, Rodger A Liddle, Nada A Abumrad
Genetic variants in the fatty acid (FA) translocase FAT/CD36 associate with abnormal postprandial lipids and influence risk for the metabolic syndrome. CD36 is abundant on apical enterocyte membranes in the proximal small intestine, where it facilitates FA uptake and FA-initiated signaling. We explored whether CD36 signaling influences FA-mediated secretion of cholecystokinin (CCK) and secretin, peptides released by enteroendocrine cells (EECs) in the duodenum/jejunum, which regulate events important for fat digestion and homeostasis...
March 2013: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Katharine V Hand, Christine M Bruen, Fiona O'Halloran, Harsh Panwar, Danielle Calderwood, Linda Giblin, Brian D Green
PURPOSE: Peptide YY (PYY) is a gastrointestinal hormone with physiological actions regulating appetite and energy homoeostasis. The cellular mechanisms by which nutrients stimulate PYY secretion from intestinal enteroendocrine cells are still being elucidated. METHODS: This study comprehensively evaluated the suitability of intestinal STC-1 cells as an in vitro model of PYY secretion. PYY concentrations (both intracellular and in culture media) with other intestinal peptides (CCK, GLP-1 and GIP) demonstrated that PYY is a prominent product of STC-1 cells...
June 2013: European Journal of Nutrition
Bhavik P Shah, Pin Liu, Tian Yu, Dane R Hansen, Timothy A Gilbertson
Fatty acid-induced stimulation of enteroendocrine cells leads to release of the hormones such as cholecystokinin (CCK) that contribute to satiety. Recently, the fatty acid activated G protein-coupled receptor GPR120 has been shown to mediate long-chain unsaturated free fatty acid-induced CCK release from the enteroendocrine cell line, STC-1, yet the downstream signaling pathway remains unclear. Here we show that linoleic acid (LA) elicits membrane depolarization and an intracellular calcium rise in STC-1 cells and that these responses are significantly reduced when activity of G proteins or phospholipase C is blocked...
January 1, 2012: American Journal of Physiology. Cell Physiology
Qi Sun, Akira Hirasawa, Takafumi Hara, Ikuo Kimura, Tetsuya Adachi, Takeo Awaji, Masaji Ishiguro, Takayoshi Suzuki, Naoki Miyata, Gozoh Tsujimoto
GPR120 is a G protein-coupled receptor expressed preferentially in the intestinal tract and adipose tissue, that has been implicated in mediating free fatty acid-stimulated glucagon-like peptide-1 (GLP-1) secretion. To develop GPR120-specific agonists, a series of compounds (denoted as NCG compounds) derived from a peroxisome proliferator-activated receptor γ agonist were synthesized, and their structure-activity relationships as GPR120 agonists were explored. To examine the agonistic activities of these newly synthesized NCG compounds, and of compounds already shown to have GPR120 agonistic activity (grifolic acid and MEDICA16), we conducted docking simulation in a GPR120 homology model that was developed on the basis of a photoactivated model derived from the crystal structure of bovine rhodopsin...
November 2010: Molecular Pharmacology
Katharine V Hand, Christine M Bruen, Fiona O'Halloran, Linda Giblin, Brian D Green
Cholecystokinin (CCK) is a peptide hormone secreted from the I-cells of the intestine and it has important physiological actions related to appetite regulation and satiety. In this study we used STC-1 cells to investigate the effects of common dietary-derived fatty acids (FAs) on I-cell secretory function and metabolism. We extend earlier studies by measuring the acute and chronic effects of 11 FAs on CCK secretion, cellular CCK content, CCK mRNA levels, cellular DNA synthesis, cellular viability and cytotoxicity...
May 2010: Molecular Nutrition & Food Research
Lei Qiao, Chun-Sheng Chen, Shu-Cheng Zhang
OBJECTIVE: To investigate the expression and activity of fatty acid amide hydrolase (FAAH) in the colon and its role in children with slow transit constipation (STC). METHODS: Patients were divided into constipation group (n=21) and control group (n=15). The constipation group was consistent with the diagnostic criteria for STC. Western blotting, immunohistochemistry and real-time PCR were used to examine the FAAH expression in surgical specimen of colon. The location and distribution of FAAH and cannabinoid receptor type 1 (CB1) were detected by immunofluorescence double staining...
January 2010: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Takafumi Hara, Akira Hirasawa, Qi Sun, Keiko Sadakane, Chisato Itsubo, Tomoyo Iga, Tetsuya Adachi, Taka-aki Koshimizu, Toshihiro Hashimoto, Yoshinori Asakawa, Gozoh Tsujimoto
GPR120 and GPR40 are G-protein-coupled receptors whose endogenous ligands are medium- and long-chain free fatty acids, and they are thought to play an important physiological role in insulin release. Despite recent progress in understanding their roles, much still remains unclear about their pharmacology, and few specific ligands for GPR120 and GPR40 besides medium- to long-chain fatty acids have been reported so far. To identify new selective ligands for these receptors, more than 80 natural compounds were screened, together with a reference compound MEDICA16, which is known to activate GPR40, by monitoring the extracellular regulated kinase (ERK) and [Ca(2+)](i) responses in inducible and stable expression cell lines for GPR40 and GPR120, respectively...
September 2009: Naunyn-Schmiedeberg's Archives of Pharmacology
M C P Geraedts, F J Troost, W H M Saris
Dietary modulation of the response of gut satiety hormones, which partly regulate food intake, provides a promising treatment for overweight and obesity. Gut-derived cell lines such as STC-1 are widely used to investigate these hormonal responses to nutrients. To date, no peptide-YY (PYY) secreting cell line has been identified. The aim of this study was to investigate whether STC-1 cells are able to secrete PYY and if so, whether dietary compounds can modulate PYY secretion. The effects of fatty acid types C4:0, C12:0, C14:0, C16:0, and C18:0 on PYY release were investigated by measuring PYY in the supernatant after 30, 60, 90, and 120 min of incubation, respectively, using RIA assays...
March 2009: Journal of Food Science
Wilrike J Pasman, Jos Heimerikx, Carina M Rubingh, Robin van den Berg, Marianne O'Shea, Luisa Gambelli, Henk F J Hendriks, Alexandra W C Einerhand, Corey Scott, Hiskias G Keizer, Louise I Mennen
Appetite suppressants may be one strategy in the fight against obesity. This study evaluated whether Korean pine nut free fatty acids (FFA) and triglycerides (TG) work as an appetite suppressant. Korean pine nut FFA were evaluated in STC-1 cell culture for their ability to increase cholecystokinin (CCK-8) secretion vs. several other dietary fatty acids from Italian stone pine nut fatty acids, oleic acid, linoleic acid, alpha-linolenic acid, and capric acid used as a control. At 50 muM concentration, Korean pine nut FFA produced the greatest amount of CCK-8 release (493 pg/ml) relative to the other fatty acids and control (46 pg/ml)...
2008: Lipids in Health and Disease
Toshiki Tanaka, Susumu Katsuma, Tetsuya Adachi, Taka-aki Koshimizu, Akira Hirasawa, Gozoh Tsujimoto
The ingestion of fat induces secretion of the gut peptide hormone cholecystokinin (CCK); however, the mechanism responsible for lipid-induced CCK release remains unknown. Recently, a group of free fatty acid (FFA) receptors, which includes the long-chain FFA receptors GPR120 and GPR40, has been identified. In this study, we examined whether these FFA receptors mediate lipid-induced CCK release in the mouse. We first observed that intra-gastric administration of long-chain FFAs increased plasma CCK levels. Using mouse enteroendocrine STC-1 cells as a model system, we further studied the mechanism of this FFA-induced CCK secretion...
June 2008: Naunyn-Schmiedeberg's Archives of Pharmacology
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