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cytometry part B clinical cytometr

Donatella Raspadori, Paola Pacelli, Anna Sicuranza, Elisabetta Abruzzese, Alessandra Iurlo, Daniele Cattaneo, Antonella Gozzini, Sara Galimberti, Claudia Baratè, Patrizia Pregno, Maura Nicolosi, Federica Sorà, Mario Annunziata, Luigiana Luciano, Giovanni Caocci, Sabrina Moretti, Nicola Sgherza, Claudio Fozza, Sabina Russo, Emilio Usala, Marina A Liberati, Sara Ciofini, Monika M Trawinska, Alessandro Gozzetti, Monica Bocchia
BACKGROUND: Recent investigations in chronic myeloid leukemia (CML) have focused on the identification and characterization of leukemic stem cells (LSCs). These cells reside within the CD34+ /CD38─ /Lin─ fraction and score positive for CD26 (dipeptidylpeptidase IV) a marker, expressed in both bone marrow (BM) and peripheral blood (PB) samples, that discriminates CML cells from normal hematopoietic stem cells (HSCs) or from LSCs of other myeloid neoplasms. CD26 evaluation could be a useful tool to improve the identification of CML LCSs by using flow-cytometry assay...
February 3, 2019: Cytometry. Part B, Clinical Cytometry
Sushmita Sinha, Michael P Crawford, Sterling B Ortega, Nitin J Karandikar
The immune system plays a major pathological and regulatory role in multiple sclerosis (MS) and, therefore, is a focus of extensive research. Animal models of MS have been crucial in understanding the pathological processes in MS and developing certain treatments, however, all crucial aspects of the human disease may not be appropriately modeled. With the exception of detecting oligoclonal bands and IgG synthesis in cerebrospinal fluids of MS patients, there has not been major progress in the development of immunologic tests that can be used for diagnosis of MS...
January 2015: Journal of Multiple Sclerosis
Kevin L Holmes, Benjamin Fontes, Philip Hogarth, Richard Konz, Simon Monard, Charles H Pletcher, Robert B Wadley, Ingrid Schmid, Stephen P Perfetto
Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument operators and the environment from biohazards arising from the use of sorters. To this end the International Society of Analytical Cytology (ISAC) was proactive in establishing biosafety guidelines in 1997 (Schmid et al., Cytometry 1997;28:99-117) and subsequently published revised biosafety standards for cell sorting of unfixed samples in 2007 (Schmid et al...
May 2014: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
L Simon, L Liu, K Murphy, S Ge, J Hotaling, K I Aston, B Emery, D T Carrell
STUDY QUESTION: Is there an association between sperm DNA damage, measured by three different assays, sperm nuclear protein content and clinical outcomes in assisted reproduction treatment (ART)? SUMMARY ANSWER: Sperm DNA damage measured by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) and the Comet assay were significantly associated with ART outcomes in our single institution study. WHAT IS KNOWN ALREADY: Abnormal protamine expression is known to be associated with sperm DNA damage and male infertility...
May 2014: Human Reproduction
Colm B Collins, Carol M Aherne, Stefan F Ehrentraut, Mark E Gerich, Eóin N McNamee, Martine C McManus, Matthew D P Lebsack, Paul Jedlicka, Tania Azam, Edwin F de Zoeten, Charles A Dinarello, Jesús Rivera-Nieves
BACKGROUND: Fecal alpha-1-antitrypsin (AAT) clearance has been a marker of clinical disease severity in inflammatory bowel diseases (IBDs) for many years. Although AAT deficiency is more often associated with lung and liver pathologies, AAT-deficient patients with concomitant IBD have been shown to develop more aggressive disease and rapid progression to surgery. Although recent studies have highlighted the pleiotropic anti-inflammatory functions of AAT, including reducing proinflammatory cytokine production and suppressing immune cell activation, its potential therapeutic role in IBD has not been described...
August 2013: Inflammatory Bowel Diseases
B Greig, M Stetler-Stevenson, J Lea
BACKGROUND: Flow cytometric immunophenotpying (FCI) of cerebrospinal fluid (CSF) and other paucicellular fluids has been demonstrated to have increased sensitivity in detection of lymphoma and leukemia when compared to cytomorphology [(1) de Graaf et al., Cytometry Part B 2011, 80B:271-281; (2) Szamosi et al., CLSI Document H56-A-Body Fluid Analysis for Cellular Composition; Approved Guideline, Wayne, PA: Clinical and Laboratory Standards Institute, 2006; (3) Kraan et al., Flow Cytometric Immunophenotyping of Cerebrospinal Fluid...
March 2014: Cytometry. Part B, Clinical Cytometry
Gert-Jan van de Geijn, Vincent van Rees, Natasja van Pul-Bom, Erwin Birnie, Hans Janssen, Hans Pegels, Marlène Beunis, Tjin Njo
Differential white blood cell count (dWBC) is a frequently used diagnostic tool. For most patient samples an automated blood counter produces a five-part differential count. If this dWBC does not meet pre-set criteria, microscopic dWBC is performed. Microscopy is labor intensive and requires sustained training of technicians. Inter-observer variation and statistical variation are significant, due to limited numbers of cells counted. Flow cytometry is a candidate reference method for dWBC. Advantages are immunological definitions and large number of measured cells...
September 2011: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
T J Kiernan, B A Boilson, L Tesmer, A Harbuzariu, R D Simari, G W Barsness
BACKGROUND: Enhanced external counterpulsation (EECP) is associated with improvement in endothelial function, angina and quality of life in patients with symptomatic coronary artery disease, although the mechanisms underlying the observed clinical benefits are not completely clear. The purpose of this study was to examine the effects of EECP on circulating haematopoietic progenitor cells (HPCs) and endothelial progenitor cells (EPCs) in patients with refractory angina. We compared HPC and EPC counts between patients scheduled for EECP and patients with normal angiographic coronary arteries, with and without coronary endothelial dysfunction...
December 1, 2011: International Journal of Cardiology
Jing Li, Huiping Liu, Srinivasan Ramachandran, Gregory B Waypa, Jun-Jie Yin, Chang-Qing Li, Mei Han, Hsien-Hao Huang, Willard W Sillard, Terry L Vanden Hoek, Zuo-Hui Shao
Doxorubicin (Dox) is one of the most widely used and successful chemotherapeutic antitumor drugs. Its clinical application is highly limited due to its cumulative dose-related cardiotoxicity. Proposed mechanisms include the generation of reactive oxygen species (ROS)-mediated oxidative stress. Therefore, reducing oxidative stress should be protective against Dox-induced cardiotoxicity. To determine whether antioxidant, grape seed proanthocyanidin extract (GSPE) attenuates Dox-induced ROS generation and protects cardiomyocytes from Dox-induced oxidant injury, cultured primary cardiomyocytes were treated with doxorubicin (Dox, 10 microM) alone or GSPE (50 microg/ml) with Dox (10 microM) for 24 hours...
2010: American Journal of Chinese Medicine
Mousumi Rahman Qazi, Zhenlei Xia, Jasna Bogdanska, Shu-Ching Chang, Dave J Ehresman, John L Butenhoff, B Dean Nelson, Joseph W DePierre, Manuchehr Abedi-Valugerdi
We have previously shown that short-term, high-dose exposure of mice to the environmentally persistent perfluorooctanoate (PFOA) results in thymic and splenic atrophy and the attenuation of specific humoral immune responses. Here we characterize the effects of a 10-day treatment with different dietary doses (1-0.001%, w/w) of perfluorooctanesulfonate (PFOS), a similar fluorochemical, on the immune system of male C57BL/6 mice. At doses greater than 0.02%, PFOS induced clinical signs of toxicity in the animals, whereas at the concentration of 0...
June 16, 2009: Toxicology
M Schwartz, R Carlson, A Tipold
Steroid-responsive meningitis-arteritis (SRMA) is a systemic inflammatory disease of juvenile to young adult dogs with a relapsing course and most prominent manifestation in the cervical meninges. The most important laboratory finding is a marked neutrophilic pleocytosis. Integrin (CD11a, b, c) expression on polymorphonuclear cells (PMNs) was quantified by immunophenotyping and subsequent flow cytometric measurements. Values were determined for peripheral blood in the acute phase of SRMA (n=14) as well as during glucocorticosteroid treatment (n=16)...
December 15, 2008: Veterinary Immunology and Immunopathology
M A Ilham, S Winkler, E Coates, A Rizzello, T J Rees, A Asderakis
Pretransplantation crossmatching is an integral part of kidney transplantation. Flow cytometric crossmatch (FCXM) is more sensitive than complement-dependent cytotoxic crossmatch (CDC-XM). However, the clinical significance of positive FCXM with negative CDC-XM is controversial. We evaluated FCXM in 455 consecutive deceased donor renal transplants. All had a negative CDC-XM. There were 341 T-cell and B-cell FCXM negative and 38 T-cell and B-cell positive. There was a higher percentage of retransplantations and HLA mismatches (26...
July 2008: Transplantation Proceedings
Charlie Mantel, Ying Guo, Man Ryul Lee, Myung Kwan Han, Sara Rhorabough, Kye Seong Kim, Hal E Broxmeyer
It is widely accepted that mammalian cells enter the next G(1)-phase (G(1)) with 4N DNA after slippage from prolonged drug-induced mitotic block caused by activation of the transient spindle checkpoint. Understanding cell fate after mitotic slippage (MS) has significant clinical importance. The conclusion the MS cells enter 4N-G(1) is based on morphology and mitotic cyclin destruction. Definitive biochemical evidence for G(1) is scarce or unconvincing, in part because of methods of protein extraction required for immunoblot analysis that cannot take into account the cell cycle heterogeneity of cell cultures...
February 15, 2008: Cell Cycle
B H Davis, J T Holden, M C Bene, M J Borowitz, R C Braylan, D Cornfield, W Gorczyca, R Lee, R Maiese, A Orfao, D Wells, B L Wood, M Stetler-Stevenson
The clinical indications for diagnostic flow cytometry studies are an evolving consensus, as the knowledge of antigenic definition of hematolymphoid malignancies and the prognostic significance of antigen expression evolves. Additionally the standard of care is not routinely communicated to practicing clinicians and diagnostic services, especially as may relate to new technologies. Accordingly there is often uncertainty on the part of clinicians, payers of medical services, diagnostic physicians and scientists as to the appropriate use of diagnostic flow cytometry...
2007: Cytometry. Part B, Clinical Cytometry
Segaran Pillai, Christine Pillai, Lester A Mitscher, Raymond Cooper
INTRODUCTION: When directly exposed to various echinacea fractions, human leukocytes ex vivo are strongly stimulated to proliferate and to produce immunostimulation and inflammatory cytokines. A comparison of fractions containing lipoidal small molecules and high-molecular-weight water-soluble polysaccharides indicates that the latter are substantially more potent as immunostimulants. Echinacea purpurea (L.) Moench, E. angustifolia DC, and E. pallida (Nutt.), Nutt. extracts, and each plant part contain significantly potent constituents...
July 2007: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
Cherie H Dunphy
CONTEXT: Gene expression (GE) analyses using microarrays have become an important part of biomedical and clinical research in hematolymphoid malignancies. However, the methods are time-consuming and costly for routine clinical practice. OBJECTIVES: To review the literature regarding GE data that may provide important information regarding pathogenesis and that may be extrapolated for use in diagnosing and prognosticating lymphomas and leukemias; to present GE findings in Hodgkin and non-Hodgkin lymphomas, acute leukemias, and chronic myeloid leukemia in detail; and to summarize the practical clinical applications in tables that are referenced throughout the text...
April 2006: Archives of Pathology & Laboratory Medicine
Jan W Gratama, Jan J Cornelissen
The construction and use of Class I human leucocyte antigen (HLA) tetramers has, for the first time, allowed the direct enumeration of CD8(+) T lymphocytes specific for the antigen of interest. Tetramer staining can be combined with functional assays of antigen-specific T cells measuring their production of intracellular cytokines after short-term stimulation with antigen. The advantages of flow cytometric tetramer-based assays are their short turn-around time and their amenability to standardisation. Currently, their main limitation is that only a limited number of Class I HLA tetramers are available...
2003: BioDrugs: Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy
E Koníková, J Kusenda
In order to define the possible role of the MDM2 gene in the pathogenesis of human leukemia, the expression of MDM2 protein was examined in samples of fixed-permeabilized peripheral blood (PB) or bone marrow (BM) cells of leukemic patients by using flow cytometry. The present study showed, that normal PB and BM cells expressed low levels of MDM2. Overexpression of this protein was more frequently found in leukemic cells, namely in samples of patients with advanced, than those in incipient clinical stage of disease at examination...
2003: Neoplasma
Peng Wang, Shu-Hui Sun, Phyllis B Silver, Chi-Chao Chan, Rajeev K Agarwal, Barbara Wiggert, Leonard D Kohn, Gordon A Jamieson, Rachel R Caspi
Methimazole (methyl-mercapto-imidazole, MMI), a compound used clinically in therapy of Graves' thyroiditis, was found to inhibit development of several autoimmune diseases in animal models. It was suggested on the basis of in vitro data that inhibition is through down-regulation of interferon-gamma (IFN-gamma)-induced expression of major histocompatibility complex class I and class II molecules. Here, we investigate the effect of MMI on experimental autoimmune uveoretinitis (EAU) and study its mechanism(s)...
January 2003: Journal of Leukocyte Biology
Xianzhi Zhang, Eeva Moilanen, Ian M Adcock, Mark A Lindsay, Hannu Kankaanranta
Mometasone is a potent synthetic glucocorticoid, which is under development as an inhaled preparation for the treatment of asthma. Previous studies have suggested that glucocorticoids have direct effects on human eosinophil and neutrophil apoptosis. The present study was designed to characterize the effects of mometasone on constitutive apoptosis and cytokine-afforded survival in isolated human eosinophils and neutrophils. The isolated eosinophils or neutrophils were cultured in vitro, and apoptosis was assessed by flow cytometric analysis of relative DNA content, by annexin-V binding and morphological analysis...
August 16, 2002: Life Sciences
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