Rodrigo G de Souza, Zhiyuan Yu, Humberto Hernandez, Claudia M Trujillo-Vargas, Andrea Lee, Kelsey E Mauk, Jiyang Cai, Milton R Alves, Cintia S de Paiva
Inflammation and oxidative stress accompany aging. This study aimed to investigate the interplay between oxidative stress and inflammation in the lacrimal gland (LG). C57BL/6 (B6) mice were used at 2-3, 12, and 24 months of age (2-3M, 12M, and 24M, respectively). Nuclear factor erythroid derived-2-related factor 2 (Nrf2)-/- and their corresponding wild-type (WT) mice were used at 2-3M and 12-13M. A separate group of 15.5-17M B6 mice received a diet containing an Nrf2 inducer (Oltipraz) for 8 weeks. Aged B6 LGs showed significantly greater focus score, higher levels of MHC II, IFN-γ, IL-1β, TNF-α, and Cathepsin S (Ctss) mRNA transcripts, and greater nitrotyrosine and 4-hydroxyneal (4-HNE) protein...
November 4, 2020: American Journal of Pathology