Read by QxMD icon Read


Brian M Moran, Michael G Miskelly, Yasser H A Abdel-Wahab, Peter R Flatt, Aine M McKillop
The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injection, oral administration of Zn2+ (50 μmol/kg body weight) with glucose (18 mmol/kg) in lean mice reduced the glycaemic excursion by 25-34% (p<0.05-p<0.001) and enhanced glucose-induced insulin release by 46-48% (p<0...
December 1, 2018: Biological Chemistry
Zhiwen Liu, Hong Liu, Li Xiao, Guoyong Liu, Lin Sun, Liyu He
Diabetic kidney disease (DKD) is the leading cause of morbidity and mortality in individuals with diabetes, and it is the leading cause of end-stage renal disease (ESRD) worldwide. Stanniocalcin-1 (STC-1) is present in various tissues, and it has antioxidant and anti-apoptotic activities, which play a role in kidney protection, including diabetic nephropathy (DN). However, the mechanism that underlies these effects remains unknown. This study suggests that STC-1 ameliorates oxidative stress and cell apoptosis in the kidneys of db/db mice and high glucose (HG)-treated BUMPT cells by inhibiting Bnip3 expression through AMPK/Sirt3 pathway activation...
January 25, 2019: Laboratory Investigation; a Journal of Technical Methods and Pathology
Pengzhou Li, Liyong Zhu, Xiangwu Yang, Weizheng Li, Xulong Sun, Bo Yi, Shaihong Zhu
Type II diabetes is a complex, chronic, and progressive disease. Glucagon-like peptide-1 (7-36) amide (GLP-1) is a gut hormone released from the L cells which stimulate insulin secretion and promotes insulin gene expression and β-cell growth and differentiation. Elevated levels of hormones secreted by L cells are an essential reason for diabetes improvement. GLP-1 secretion has been reported to be regulated by farnesoid X receptor (FXR), a transcriptional sensor for bile acids which also acts on glucose metabolism...
December 7, 2018: Journal of Cellular Physiology
David Planes-Muñoz, Rubén López-Nicolás, Carlos A González-Bermúdez, Gaspar Ros-Berruezo, Carmen Frontela-Saseta
Plant extract activity can be modified by the digestion process. In order to assess the satiety effect of green tea and turmeric extracts, an in vitro gastrointestinal digestion process was performed and the STC-1 cell line was used. The enteroendocrine cell line was incubated (for 30, 60 and 120 minutes) with the digested and non-digested extracts measuring the secretion of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) by enzyme-linked immunosorbent assay (ELISA). The release of satiety hormones by the STC-1 cells showed similar or higher results for plant extracts compared to the positive controls reported as satiatogenic (proteins and short chain fatty acids)...
October 17, 2018: Food & Function
Marta Santos-Hernández, Daniel Tomé, Claire Gaudichon, Isidra Recio
The present study evaluates casein and whey protein gastrointestinal digests as inducers of CCK and GLP-1 secretion and expression in STC-1 cells. In vitro digests were characterized regarding protein, peptide and free amino acid content. Digests from the intestinal phase containing small size peptides and free amino acids behaved as more potent CCK inducers than digests from the gastric phase. However, GLP-1 release was maximized with casein gastric digests and whey protein intestinal digests. Human jejunal digests from the same substrates showed a comparable response, except for casein jejunal digests which exerted a higher effect than in vitro casein gastrointestinal digests...
September 19, 2018: Food & Function
Pengzhou Li, Liyong Zhu, Xiangwu Yang, Weizheng Li, Xulong Sun, Bo Yi, Shaihong Zhu
BACKGROUND/AIMS: Type II diabetes is a complex, chronic, and progressive disease. Glucagon-like peptide-1 (7-6) amide (GLP-1) is a gut hormone released from the L cells which stimulates insulin secretion, and promotes insulin gene expression and β-cell growth and differentiation. Elevated levels of hormone secreted by L cells are an important reason for diabetes improvement. GLP-1 secretion has been reported to be regulated by farnesoid X receptor (FXR), a transcriptional sensor for bile acids which also acts on glucose metabolism...
2018: Cellular Physiology and Biochemistry
Tohru Hira, Shono Ogasawara, Asuka Yahagi, Minami Kamachi, Jiaxin Li, Saki Nishimura, Masayoshi Sakaino, Takatoshi Yamashita, Shigenobu Kishino, Jun Ogawa, Hiroshi Hara
SCOPE: The secretion of gut hormones, such as cholecystokinin (CCK) is stimulated by fatty acids. Although a chain length-dependent mechanism has been proposed, other structural relationships to releasing activity remain unclear. We aimed to elucidate specific structures in fatty acids that are responsible for their CCK-releasing activity, and related sensing mechanisms in enteroendocrine cells. METHODS AND RESULTS: CCK secretory activities were examined in a murine CCK-producing cell line STC-1 by exposing the cells to various modified fatty acids produced by gut lactic acid bacteria...
October 2018: Molecular Nutrition & Food Research
Hening Zhai, Zhi Li, Miao Peng, Zhaoqi Huang, Tingfeng Qin, Linxi Chen, Hanbing Li, Heng Zhang, Weizhen Zhang, Geyang Xu
BACKGROUND: The mechanism by which Roux-en-Y Gastric Bypass (RYGB) increases the secretion of glucagon-like peptide-1 (GLP-1) remains incompletely defined. Here we investigated whether TGR5-mTORC1 signaling mediates the RYGB-induced alteration in GLP-1 production in mice and human beings. METHODS: Circulating bile acids, TGR5-mTORC1 signaling, GLP-1 synthesis and secretion were determined in lean or obese male C57BL/6 mice with or without RYGB operation, as well as in normal glycemic subjects, obese patients with type 2 diabetes before and after RYGB...
June 2018: EBioMedicine
Yuefeng Zhang, Shifei Huang, Pengping Li, Qing Chen, Yongzhou Li, Yizhao Zhou, Lantian Wang, Muxing Kang, Bo Zhang, Bin Yang, Xin Dong, Yulian Wu
One hallmark of pancreatic cancer (PC) is the high prevalence of pancreatic cancer-associated diabetes mellitus (PC-DM), but the mechanisms remain to be elucidated. Patients with PC who are diagnosed with new-onset diabetes/prediabetes have recently been shown to display significantly lower levels of glucose-dependent insulinotropic peptide (GIP) secreted mainly by enteroendocrine cells. We hypothesized that PC-derived exosomes are responsible for the decreased levels of incretins in patients with PC-DM. In this study, exosomes were successfully isolated from PANC-1, MIA PaCa-2 and SW620 cells and characterized...
September 1, 2018: Cancer Letters
Sheng Huang, Yayun Chi, Yi Qin, Ziliang Wang, Bingqiu Xiu, Yonghui Su, Rong Guo, Liang Guo, Hefen Sun, Chujia Zeng, Shuling Zhou, Xin Hu, Sheng Liu, Zhimin Shao, Zhaohui Wu, Wei Jin, Jiong Wu
Macrophage-capping protein (CAPG) has been shown to promote cancer cell metastasis, although the mechanism remains poorly understood. Methods: Breast cancer (BC) tissue microarray was used to test the role of CAPG in the prognosis of BC patients. Xenograft mice model was used to validate the metastasis promotion role of CAPG in vivo. Gene expression array, chromatin immunoprecipitation and luciferase report assay were performed to search for the target genes of CAPG. Protein immunoprecipitation, MS/MS analysis, tissue microarray and histone methyltransferase assay were used to explore the mechanism of CAPG regulating stanniocalcin 1 (STC-1) transcription...
2018: Theranostics
L Shcherbina, A Lindqvist, A-H Thorén Fischer, E Ahlqvist, E Zhang, S E Falkmer, E Renström, J Koffert, H Honka, N Wierup
Impaired incretin effect is a culprit in Type 2 Diabetes. Cocaine- and amphetamine-regulated transcript (CART) is a regulatory peptide controlling pancreatic islet hormone secretion and beta-cell survival. Here we studied the potential expression of CART in enteroendocrine cells and examined the role of CART as a regulator of incretin secretion and expression. CART expression was found in glucose-dependent insulinotropic polypeptide (GIP)-producing K-cells and glucagon-like peptide-1 (GLP-1)-producing L-cells in human duodenum and jejunum and circulating CART levels were increased 60 min after a meal in humans...
November 15, 2018: Molecular and Cellular Endocrinology
Min Zhao, Wankun Xie, Shu-Huai Tsai, Travis W Hein, Brent A Rocke, Lih Kuo, Robert H Rosa
Purpose: The purpose of this study was to investigate the impact of stanniocalcin-1 (STC-1), a photoreceptor-protective glycoprotein, on the development of choroidal neovascularization (CNV) in relation to VEGF and its main receptor (VEGFR2) expression after laser injury. Methods: In rats, CNV was induced by laser photocoagulation in both eyes, followed by intravitreal injection of STC-1 in the right eye and vehicle or denatured STC-1 injection in the left eye as control...
February 1, 2018: Investigative Ophthalmology & Visual Science
Fiona O'Halloran, Christine Bruen, Brian McGrath, Harriët Schellekens, Brian Murray, John F Cryan, Alan L Kelly, Paul L H McSweeney, Linda Giblin
In an effort to control weight gain, much attention has focused on the identification of bioactive peptides from food sources that induce satiety hormone secretion and increase the feeling of fullness. In this study, a screening platform identified a sodium caseinate hydrolysate, LFC25, that significantly increased calcium signalling in the enteroendocrine cell line, STC-1, and as a result increased secretion of the satiety hormone, GLP-1, in a dose-dependent manner. Administration of this hydrolysate to mice reduced the cumulative food intake over an eight hour period...
June 30, 2018: Food Chemistry
Jesse Zhan, Jonathan Weng, Brian G Hunt, W Sean Davidson, Min Liu, Chunmin C Lo
Cholecystokinin (CCK) and apolipoprotein A-IV (ApoA-IV) are gastrointestinal peptides that play an important role in controlling energy homeostasis. Lymphatic ApoA-IV and plasma CCK secretion are mediated via a chylomicron formation-dependent pathway during a dietary lipid infusion. Given their similar roles as satiating proteins, the present study examines how the two peptides interact in their function. Specifically, this study sought to understand how ApoA-IV regulates CCK secretion. For this purpose, Cck gene expression in the small intestines of ApoA-IV knockout (ApoA-IV-KO) and wild-type (WT) mice were compared under an array of feeding conditions...
May 1, 2018: Physiology & Behavior
Xiao Yue, Jie Liang, Fu Gu, Dongshu Du, Fuxue Chen
Glucagon-like peptide-1 (GLP-1) is involved in the regulation of insulin secretion and glucose homeostasis. GLP-1 release is stimulated when berberine interacts with a novel G protein family (TAS2Rs) in enteroendocrine cells. In this study, we used STC-1 cells and examined a marked increase in Ca2+ in response to various bitter compounds. Ca2+ responses to traditional Chinese medicine extracts, including berberine, phellodendrine and coptisine, in STC-1 cells were suppressed by the phospholipase C (PLC) inhibitor U-73122, suggesting the involvement of bitter taste receptors in changing the physiological status of enteroendocrine cells in a PLC-dependent manner...
October 2018: Molecular and Cellular Biochemistry
Dipak Dilip Chaudhari, Rajbir Singh, Rashmi Hogarehalli Mallappa, Namita Rokana, Jai Kumar Kaushik, Rajesh Bajaj, Virender Kumar Batish, Sunita Grover
BACKGROUND & OBJECTIVES: Milk proteins play a beneficial role in the regulation of food intake, postprandial glycaemia and enteroendocrine hormone secretions and thus are receiving considerable attention for the management of metabolic inflammatory disorders such as type 2 diabetes mellitus (T2DM). The objective of this study was to evaluate the efficacy of peptide/s obtained from milk proteins (casein and whey) as well as from the milk fermented with Lactobacillus helveticus as secretagogues for gut hormones and to purify and characterize the active peptides...
September 2017: Indian Journal of Medical Research
Fei Wang, Xiudao Song, Liang Zhou, Guoqiang Liang, Fei Huang, Guorong Jiang, Lurong Zhang
CONTEXT: Sweet taste receptors (STRs) involve in regulating the release of glucose-stimulated glucagon-like peptide-1 (GLP-1). Our in vivo and in vitro studies found that 3-deoxyglucosone (3DG) inhibited glucose-stimulated GLP-1 secretion. OBJECTIVE: This study investigated the role of STRs in 3DG-induced inhibition of high glucose-stimulated GLP-1 secretion. METHODS: STC-1 cells were incubated with lactisole or 3DG for 1 h under 25 mM glucose conditions...
December 26, 2017: Archives of Physiology and Biochemistry
Liusong Yang, Lina Wang, Canjun Zhu, Junguo Wu, Yexian Yuan, Lulu Yu, Yaqiong Xu, Jingren Xu, Tao Wang, Zhengrui Liao, Songbo Wang, Xiaotong Zhu, Ping Gao, Yongliang Zhang, Xiuqi Wang, Qingyan Jiang, Gang Shu
Laminarin, a type of β-glucan isolated from brown seaweeds, exhibits verity of physiological activities, which include immunology modulation and antitumor function. To investigate the effect of laminarin on energy homeostasis, mice were orally administrated with laminarin to test food intake, fat deposition, and glucose homeostasis. Chronically, laminarin treatment significantly decreases high-fat-diet-induced body weight gain and fat deposition and reduces blood glucose level and glucose tolerance. Acutely, laminarin enhances serum glucagon-like peptide-1 (GLP-1) content and the mRNA expression level of proglucagon and prohormone convertase 1 in ileum...
November 21, 2017: Oncotarget
Linda Sundström, Susanna Myhre, Monika Sundqvist, Andrea Ahnmark, William McCoull, Piotr Raubo, Sam D Groombridge, Magnus Polla, Ann-Christin Nyström, Lisbeth Kristensson, Mats Någård, Maria Sörhede Winzell
The mechanism behind the glucose lowering effect occurring after specific activation of GPR120 is not completely understood. In this study, a potent and selective GPR120 agonist was developed and its pharmacological properties were compared with the previously described GPR120 agonist Metabolex-36. Effects of both compounds on signaling pathways and GLP-1 secretion were investigated in vitro. The acute glucose lowering effect was studied in lean wild-type and GPR120 null mice following oral or intravenous glucose tolerance tests...
2017: PloS One
Gavin W Roddy, Douglas Yasumura, Michael T Matthes, Marcel V Alavi, Sanford L Boye, Robert H Rosa, Michael P Fautsch, William W Hauswirth, Matthew M LaVail
Retinal degenerations, including age-related macular degeneration and the retinitis pigmentosa family of diseases, are among the leading causes of legal blindness in the United States. We previously found that Stanniocalcin-1 (STC-1) reduced photoreceptor loss in the S334ter-3 and Royal College of Surgeons rat models of retinal degeneration. The results were attributed in part to a reduction in oxidative stress. Herein, we tested the hypothesis that long-term delivery of STC-1 would provide therapeutic rescue in more chronic models of retinal degeneration...
December 2017: Experimental Eye Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"