keyword
MENU ▼
Read by QxMD icon Read
search

vibrio pathogen

keyword
https://read.qxmd.com/read/30782660/evolutionary-model-of-cluster-divergence-of-the-emergent-marine-pathogen-vibrio-vulnificus-from-genotype-to-ecotype
#1
Mario López-Pérez, Jane M Jayakumar, Jose M Haro-Moreno, Asier Zaragoza-Solas, Geethika Reddi, Francisco Rodriguez-Valera, Orr H Shapiro, Munirul Alam, Salvador Almagro-Moreno
Vibrio vulnificus , an opportunistic pathogen, is the causative agent of a life-threatening septicemia and a rising problem for aquaculture worldwide. The genetic factors that differentiate its clinical and environmental strains remain enigmatic. Furthermore, clinical strains have emerged from every clade of V. vulnificus In this work, we investigated the underlying genomic properties and population dynamics of the V. vulnificus species from an evolutionary and ecological point of view. Genome comparisons and bioinformatic analyses of 113  V...
February 19, 2019: MBio
https://read.qxmd.com/read/30778145/export-of-a-vibrio-parahaemolyticus-toxin-by-the-sec-and-type-iii-secretion-machineries-in-tandem
#2
Shigeaki Matsuda, Ryu Okada, Sarunporn Tandhavanant, Hirotaka Hiyoshi, Kazuyoshi Gotoh, Tetsuya Iida, Toshio Kodama
Many Gram-negative pathogens utilize dedicated secretion systems to export virulence factors such as exotoxins and effectors1-4 . Several exotoxins are synthesized as precursors containing amino-terminal Sec signal peptides and are exported through the inner-membrane-bound Sec machinery to the periplasm, followed by secretion across the outer membrane to the exterior using a type II secretion system (T2SS)3,5 . Here, we report that thermostable direct haemolysin (TDH), an exotoxin of the food-borne pathogen Vibrio parahaemolyticus, can be exported through the type III secretion system (T3SS), which engages in one-step secretion of effectors4 , despite possessing a Sec signal peptide and being mainly secreted via the T2SS...
February 18, 2019: Nature Microbiology
https://read.qxmd.com/read/30774628/identification-of-in-vivo-essential-genes-of-vibrio-vulnificus-for-establishment-of-wound-infection-by-signature-tagged-mutagenesis
#3
Kohei Yamazaki, Takashige Kashimoto, Mio Morita, Takehiro Kado, Kaho Matsuda, Moeko Yamasaki, Shunji Ueno
Vibrio vulnificus can cause severe necrotic lesions within a short time. Recently, it has been reported that the numbers of wound infection cases in healthy hosts are increasing, for which surgical procedures are essential in many instances to eliminate the pathogen owing to its rapid proliferation. However, the mechanisms by which V. vulnificus can achieve wound infection in healthy hosts have not been elucidated. Here, we advance a systematic understanding of V. vulnificus wound infection through genome-wide identification of the relevant genes...
2019: Frontiers in Microbiology
https://read.qxmd.com/read/30767412/the-regulation-of-oxidative-phosphorylation-pathway-on-vibrio-alginolyticus-adhesion-under-adversities
#4
Li Huang, Lixing Huang, Lingmin Zhao, Yingxue Qin, Yongquan Su, Qingpi Yan
Vibrio alginolyticus is one of the most important pathogens in mariculture and leading to heavy losses. After treatment with Cu2+ , Pb2+ , and low pH, the expression of oxidative phosphorylation pathway genes, including coxA, coxB, coxC, ccoN, ccoO, and ccoQ, was found commonly downregulated by RNA-seq as well as quantitative real-time PCR. RNAi significantly reduced the expression of coxA, coxB, coxC, ccoN, ccoO, and ccoQ in V. alginolyticus. Compared with the wild-type strain, the adhesion abilities of RNAi strains of V...
February 14, 2019: MicrobiologyOpen
https://read.qxmd.com/read/30763616/synergistic-effect-of-a-combined-live-vibrio-anguillarum-and-edwardsiella-piscicida-vaccine-in-turbot
#5
Pengcheng Bao, Xiang Sun, Qin Liu, Yuanxing Zhang, Xiaohong Liu
In aquaculture, more than one pathogen usually be isolated from the sick fish, creating an urgent need for developing combined vaccines to control fish disease caused by multiple pathogens simultaneously. In our previous work, two live attenuated vaccines against Vibrio anguillarum and Edwardsiella piscicida were vaccinated in turbot, exhibiting an efficient protection. However, some immunological processes such as antigenic competition, antigenic cross-reaction and antigen induced suppression during combined vaccination are unknown...
February 11, 2019: Fish & Shellfish Immunology
https://read.qxmd.com/read/30762152/stenotrophomonas-maltophilia-ahl-degrading-strains-isolated-from-marine-invertebrate-microbiota-attenuate-the-virulence-of-pectobacterium-carotovorum-and-vibrio-coralliilyticus
#6
José Carlos Reina, Marta Torres, Inmaculada Llamas
Many Gram-negative aquacultural and agricultural pathogens control virulence factor expression through a quorum-sensing (QS) mechanism involving the production of N-acylhomoserine (AHL) signalling molecules. Thus, the interruption of QS systems by the enzymatic degradation of signalling molecules, known as quorum quenching (QQ), has been proposed as a novel strategy to combat these infections. Given that the symbiotic bacteria of marine invertebrates are considered to be an important source of new bioactive molecules, this study explores the presence of AHL-degrading bacteria among 827 strains previously isolated from the microbiota of anemones and holothurians...
February 14, 2019: Marine Biotechnology
https://read.qxmd.com/read/30761714/ecological-implications-of-gene-regulation-by-tfox-and-tfoy-among-diverse-vibrio-species
#7
Lisa C Metzger, Noémie Matthey, Candice Stoudmann, Esther J Collas, Melanie Blokesch
Bacteria of the genus Vibrio are common members of aquatic environments where they compete with other prokaryotes and defend themselves against grazing predators. A macromolecular protein complex called the type VI secretion system (T6SS) is used for both purposes. Previous research showed that the sole T6SS of the human pathogen V. cholerae is induced by extracellular (chitin) or intracellular (low c-di-GMP levels) cues and that these cues lead to distinctive signalling pathways for which the proteins TfoX and TfoY serve as master regulators...
February 14, 2019: Environmental Microbiology
https://read.qxmd.com/read/30760940/acetic-acid-remediation-of-anthropogenic-contamination-of-water-at-the-gbnerr-in-mississippi
#8
Ibrahim O Farah, Willis O Lyons, Zikri Arslan, Michelle Tucci, Paul B Tchounwou
Grand Bay National Estuarine Research Reserve (GBNERR) is an important ecosystem in the Mississippi Gulf Coast. The GBNERR may be a potential source for contamination with anthropogenic bacterial pathogens that may play a significant role in the causation of waterborne human diseases. The objective of this study was to evaluate the interaction of physicochemical and microbiological water quality parameters at the GBNERR to determine quantitative levels and establish the potential for remediation of post-contamination of water and seafood by human fecal pollution from anthropogenic sources at the reserve...
April 2018: Biomedical Sciences Instrumentation
https://read.qxmd.com/read/30753891/expression-and-active-testing-of-vp7-from-gcrv-grass-carp-reovirus-fused-with-cholera-toxin-b-subunit-in-rice-calli
#9
Qiusheng Zhang, Binglian Xu, Jiajia Pan, Danyang Liu, Ruoxian Lv, Dongchun Yan
Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass carp (Ctenopharyngodon idellus) production and results in high mortality in China. VP7 from GCRV is involved in viral infection and could be suitable for developing vaccines for the control of GCRV infection. To obtain a genetically engineered vaccine and a plant-based oral vaccine and to evaluate their immune efficacy as an oral vaccine against GCRV, cholera toxin B subunit (CTB) of Vibrio cholerae fused to VP7 (CTB-VP7) was transformed into BL21(DE3) for expression...
February 9, 2019: Protein Expression and Purification
https://read.qxmd.com/read/30753635/high-genetic-diversity-of-vibrio-parahaemolyticus-isolated-from-tidal-water-and-mud-of-southern-coast-of-south-korea
#10
Doris Y W Di, Hansub Shin, Dukki Han, Tatsuya Unno, Hor-Gil Hur
A horizontal, fluorophore-enhanced, repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique (HFERP) was adapted to examine the genotypic richness and source differentiation of Vibrio parahaemolyticus (n = 1,749) isolated from tidal water and mud of southern coast of South Korea. The number of unique genotypes observed from June (163, 51.9%), September (307, 63.9%), December (205, 73.8%), and February (136, 74.7%), indicating a high degree of genetic diversity. Contrary, lower genetic diversity was detected in April (99, 46...
February 7, 2019: FEMS Microbiology Ecology
https://read.qxmd.com/read/30742313/quorum-sensing-is-required-for-full-virulence-of-vibrio-campbellii-towards-tiger-grouper-epinephelus-fuscoguttatus-larvae
#11
Noorashikin Md Noor, Tom Defoirdt, Norfarrah Alipiah, Murni Karim, Hassan Daud, Ikhsan Natrah
The link between quorum sensing in Vibrio campbellii and its virulence towards tiger grouper (Epinephelus fuscoguttatus) was investigated using V. campbellii wild type and quorum-sensing mutants with inactive quorum sensing or constitutively maximal quorum-sensing activity, and signal molecule synthase mutants. The results showed that wild-type V. campbellii is pathogenic to grouper larvae, causing more than 50% mortality after 4 days of challenge. Furthermore, the mortality of larvae challenged with the mutant with maximally active quorum sensing was significantly higher than that of larvae challenged with the wild type, whereas a higher survival was observed in the larvae challenged to the mutant with a completely inactive quorum-sensing system...
February 11, 2019: Journal of Fish Diseases
https://read.qxmd.com/read/30741961/particle-diffusometry-an-optical-detection-method-for-vibrio-cholerae-presence-in-environmental-water-samples
#12
Katherine N Clayton, Taylor J Moehling, Dong Hoon Lee, Steven T Wereley, Jacqueline C Linnes, Tamara L Kinzer-Ursem
There is a need for a rapid, robust, and sensitive biosensor to identify low concentrations of pathogens in their native sample matrix without enrichment or purification. Nucleic acid-based detection methods are widely accepted as the gold standard in diagnostics, but robust detection of low concentrations of pathogens remains challenging. Amplified nucleic acids produce more viscous solutions, which can be measured by combining these products with fluorescent particles and measuring the change in the particle diffusion coefficient using a technique known as particle diffusometry...
February 11, 2019: Scientific Reports
https://read.qxmd.com/read/30739247/an-outbreak-of-gastroenteritis-associated-with-gii-17-norovirus-contaminated-secondary-water-supply-system-in-wuhan-china-2017
#13
Xuan Zhou, De-Guang Kong, Jing Li, Bei-Bei Pang, Ying Zhao, Jun-Bo Zhou, Ting Zhang, Jun-Qiang Xu, Nobumichi Kobayashi, Yuan-Hong Wang
A gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses and pathogenic enteric bacteria by real-time RT-PCR and culture, respectively. Nucleotide sequences were determined by RT-PCR and direct sequencing...
February 9, 2019: Food and Environmental Virology
https://read.qxmd.com/read/30736316/pathogenic-and-non-pathogenic-microorganisms-in-the-rapid-alert-system-for-food-and-feed
#14
Marcin Pigłowski
The most frequently notified pathogenic microorganisms in the RASFF in 1980⁻2017 were Salmonella sp., Listeria , Escherichia and Vibrio , whereas, among the notified non-pathogenic microorganisms were unspecified microorganisms, Enterobacteriaceae, Salmonella sp. and Coliforms. Microorganisms were reported mainly in poultry meat, meat, fish, molluscs, crustaceans, fruits, vegetables, herbs, spices, nuts, milk, cereals (in food) and in feed materials and pet food (in feed). The number of notifications decreased at the turn of 2005 and 2006, but has steadily increased since then...
February 6, 2019: International Journal of Environmental Research and Public Health
https://read.qxmd.com/read/30735978/insight-into-the-diversity-of-antibiotic-resistance-genes-in-the-intestinal-bacteria-of-shrimp-penaeus-vannamei-by-culture-dependent-and-independent-approaches
#15
Kexin Liu, Jiamin Han, Surong Li, Liangting Liu, Weitie Lin, Jianfei Luo
Antibiotic resistance genes (ARGs) that distributed in antibiotic resistant bacteria (ARBs) are widespread in aquaculture and have great threats to the aquatic organism as well as to human. However, our understanding about the risk of ARGs to the health of aquatic organism is still limited. In the present study, we got a deep insight into the diversity of ARGs in the intestinal bacteria of shrimp by culture-dependent and independent approaches. Results of the PCR-based detection and culture-dependent analysis indicated that the tetracycline, sulfadiazine, quinolone and erythromycin resistance genes were prevalent in the commercial shrimps that bought from aquatic markets or supermarket...
February 5, 2019: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/30734601/inhibition-of-bacterial-%C3%AE-%C3%AE-and-%C3%AE-class-carbonic-anhydrases-with-selenazoles-incorporating-benzenesulfonamide-moieties
#16
Andrea Angeli, Mariana Pinteala, Stelian S Maier, Sonia Del Prete, Clemente Capasso, Bogdan C Simionescu, Claudiu T Supuran
A series of benzenesulfonamides incorporating selenazoles with diverse substitution patterns were investigated as inhibitors of six bacterial carbonic anhydrases (CAs, EC 4.2.1.1) from bacterial pathogens, such as Helicobacter pylori (hpCAα was the investigated enzyme), Vibrio cholerae (all the three CAs from this pathogen were considered, VchCAα, VchCAβ and VchCAγ) and Burkholderia pseudomallei (with its two CAs, BpsCAβ and BpsCAγ). All these sulfonamides were effective CA inhibitors, with potencies in the low micromolar or submicromolar range, making them attractive as lead compounds for designing antibacterials with a novel mechanism of action, which could counteract the extensive resistance problem observed with many clinically used antibiotics...
December 2019: Journal of Enzyme Inhibition and Medicinal Chemistry
https://read.qxmd.com/read/30721777/vibrio-parahaemolyticus-flagellin-induces-cytokines-expression-via-toll-like-receptor-5-pathway-in-orange-spotted-grouper-epinephelus-coioides
#17
Liangge He, Yaosi Liang, Xue Yu, Wan Peng, Jianan He, Lijun Fu, Haoran Lin, Yong Zhang, Danqi Lu
Vibrio parahaemolyticus is the major pathogen of vibriosis in aquatic animals and causes inflammation that may be related to tissue damage. Here, we have established a V. parahaemolyticus flagellin stimulation model using grouper spleen (GS) cell line. Purified V. parahaemolyticus flagellin was used to stimulate GS cells. Our results showed that the mRNA levels of orange-spotted grouper (Epinephelus coioides) toll-like receptor 5M (EcTLR5M), EcTLR5S and downstream cytokines, such as interferon-γ2 (IFN-γ2), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were all significantly increased after stimulation with V...
February 2, 2019: Fish & Shellfish Immunology
https://read.qxmd.com/read/30721047/colorimetric-aptasensor-based-on-truncated-aptamer-and-trivalent-dnazyme-for-vibrio-parahemolyticus-determination
#18
Yuhan Sun, Nuo Duan, Pengfei Ma, Yao Liang, Xiaoyin Zhu, Zhouping Wang
In this work, after optimizing the original aptamer sequence by truncation and site-directed mutagenesis, a simple and sensitive colorimetric aptasensor was established for detecting the widespread food-borne pathogen Vibrio parahemolyticus ( V. parahemolyticus). The detection strategy was based on the competition for an V. parahemolyticus specific aptamer between its complementary DNA (cDNA) and V. parahemolyticus. The aptamer-conjugated magnetic nanoparticles (MNPs) were used as capture probes, and the G-quadruplex (G4) DNAzyme was employed as signal amplifying element...
February 5, 2019: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/30716630/storm-runoff-differentially-influences-the-nutrient-concentrations-and-microbial-contamination-at-two-distinct-beaches-in-northern-china
#19
Yike He, Yaodong He, Biswarup Sen, Hao Li, Jiaqian Li, Yongfeng Zhang, Jianle Zhang, Sunny C Jiang, Guangyi Wang
With the escalating coastal development and loss of vegetated landscape, the volume of storm runoff increases significantly in Chinese coastal cities. To protect human health and valuable recreational resources, it is necessary to develop a quantitative understanding of coastal pollution. Here we studied the influence of storm runoff on the nutrients and microbial pathogens at two popular bathing beaches in northern China. Dongshan Beach, located near the mouth of an urban river, is influenced by non-point source pollution while Tiger-Rock Beach, a coastal beach, is primarily influenced by a point source from a storm drain outfall...
January 29, 2019: Science of the Total Environment
https://read.qxmd.com/read/30712137/coupled-changes-of-bacterial-community-and-function-in-the-gut-of-mud-crab-scylla-paramamosain-in-response-to-baimang-disease
#20
Yiqin Deng, Changhong Cheng, Jiawei Xie, Songlin Liu, Hongling Ma, Juan Feng, Youlu Su, Zhixun Guo
Increasing evidence has revealed a close association between intestinal bacterial community and hosts health. However, it is unclear whether and what extend Baimang disease alters the intestinal microbiota in mud crab (Scylla paramamosain). Here, we conducted intestinal contents Illumina sequencing of healthy and Baimang diseased mud crab (S. paramamosain) to understand bacterial community variations among health status. In addition, bacterial functional predication was used to investigate whether and how the bacteria variations further change their functions? The phyla of Proteobacteria, Fusobacteria, Cyanobacteria, Tenericutes, Firmicutes, Bacteroidetes, and Spirochaetae constituted over 96...
February 2, 2019: AMB Express
keyword
keyword
163292
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"