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exosome esterase

Hesam Saghaei Bagheri, Monireh Mousavi, Aysa Rezabakhsh, Jafar Rezaie, Seyed Hossein Rasta, Alireza Nourazarian, Çigir Biray Avci, Habib Tajalli, Mehdi Talebi, Ahmad Oryan, Majid Khaksar, Masoumeh Kazemi, Seyed Mahdi Nassiri, Shahrooz Ghaderi, Bakiye Goker Bagca, Reza Rahbarghazi, Emel Sokullu
The distinct role of low-level laser irradiation (LLLI) on endothelial exosome biogenesis remains unclear. We hypothesize that laser irradiation of high dose in human endothelial cells (ECs) contributes to the modulation of exosome biogenesis via Wnt signaling pathway. When human ECs were treated with LLLI at a power density of 80 J/cm2 , the survival rate reduced. The potential of irradiated cells to release exosomes was increased significantly by expressing genes CD63, Alix, Rab27a, and b. This occurrence coincided with an enhanced acetylcholine esterase activity, pseudopodia formation, and reduced zeta potential value 24 h post-irradiation...
July 2018: Lasers in Medical Science
Chang Lau, Yong Kim, David Chia, Nadine Spielmann, Guido Eibl, David Elashoff, Fang Wei, Yi-Ling Lin, Aune Moro, Tristan Grogan, Samantha Chiang, Eric Feinstein, Christopher Schafer, James Farrell, David T W Wong
Recent studies have demonstrated that discriminatory salivary biomarkers can be readily detected upon the development of systemic diseases such as pancreatic cancer, breast cancer, lung cancer, and ovarian cancer. However, the utility of salivary biomarkers for the detection of systemic diseases has been undermined due to the absence of the biological and mechanistic rationale as to why distal diseases from the oral cavity would lead to the development of discriminatory biomarkers in saliva. Here, we examine the hypothesis that pancreatic tumor-derived exosomes are mechanistically involved in the development of pancreatic cancer-discriminatory salivary transcriptomic biomarkers...
September 13, 2013: Journal of Biological Chemistry
Robert Gastpar, Mathias Gehrmann, Maria A Bausero, Alexzander Asea, Catharina Gross, Josef A Schroeder, Gabriele Multhoff
Detergent-soluble membrane vesicles are actively released by human pancreas (Colo-/Colo+) and colon (CX-/CX+) carcinoma sublines, differing in their capacity to present heat shock protein 70 (Hsp70)/Bag-4 on their plasma membranes. Floating properties, acetylcholine esterase activity, and protein composition characterized them as exosomes. An enrichment of Rab-4 documented their intracellular transport route from early endosomes to the plasma membrane. After solubilization, comparable amounts of cytosolic proteins, including tubulin, Hsp70, Hsc70, and Bag-4, but not ER-residing Grp94 and calnexin, were detectable in tumor-derived exosomes...
June 15, 2005: Cancer Research
Zhihong Cheng, Yuying Liu, Chernhoe Wang, Roy Parker, Haiwei Song
Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p...
October 2004: Protein Science
B Bodey, B Bodey, H E Kaiser
During mammalian ontogenesis, the thymic "pure" endodermal epithelial anlage develops and differentiates into a complex cellular microenvironment. Beginning the 7-8th week of intrauterine development, thymic epithelial cells chemotactically regulate (induce) numerous waves of migration of stem cells into the thymus, including the CD34+, yolk sac-derived, committed hematopoietic stem cells. In vitro experiments have established that CD34+ CD38dim human thymocytes differentiate into T lymphocytes when co-cultured with mouse fetal thymic organs...
July 1997: In Vivo
R M Johnstone, J Ahn
During the maturation of the mammalian reticulocyte to the erythrocyte, 50nm bodies (called exosomes) are released from the cell. These exosomes carry the transferrin receptor (TFR) as well as other plasma membrane and lysosomal activities which the cells are known to lose during maturation. Using an antibody attached to an iron core bead, we have shown that other plasma membrane activities (nucleoside transporter and acetylcholine esterase) are packaged in the same exosome as the transferrin receptor. This population of exosomes is devoid of lysosomal enzymes...
1990: Biomedica Biochimica Acta
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