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Outer membrane vesicle

Adam C Cooke, Alexander V Nello, Robert K Ernst, Jeffrey W Schertzer
Outer Membrane Vesicles (OMVs) are ubiquitous in bacterial environments and enable interactions within and between species. OMVs are observed in lab-grown and environmental biofilms, but our understanding of their function comes primarily from planktonic studies. Planktonic OMVs assist in toxin delivery, cell-cell communication, horizontal gene transfer, small RNA trafficking, and immune system evasion. Previous studies reported differences in size and proteomic cargo between planktonic and agar plate biofilm OMVs, suggesting possible differences in function between OMV types...
2019: PloS One
Paul D Veith, Michelle D Glew, Dhana G Gorasia, Dina Chen, Neil M O'Brien-Simpson, Eric C Reynolds
The identification and localisation of outer membrane proteins (Omps) and lipoproteins in pathogenic treponemes such as T. denticola (periodontitis) and T. pallidum (syphilis) has been challenging. In this study, label-free quantitative proteomics using MaxQuant was applied to naturally produced outer membrane vesicles (OMVs) and cellular fractions to identify 1448 T. denticola proteins. Of these, 90 proteins were localised to the outer membrane (OM) comprising 59 lipoproteins, 25 β-barrel proteins and six other putative OM-associated proteins...
February 14, 2019: Journal of Proteome Research
Simone C Barbosa, Thatyane M Nobre, Diogo Volpati, Eduardo M Cilli, Daniel S Correa, Osvaldo N Oliveira
Antimicrobial peptides are a promising class of new antibiotics with the ability to kill bacteria by disrupting their cell membrane, which is especially difficult for Gram-negative bacteria whose cell wall contains an outer layer of lipopolysaccharides (LPS). Here we show that the cyclic decapeptide Labaditin (Lo), with proven activity against the Gram-positive Staphylococcus aureus and Streptococcus mutans, is not able to kill the Gram-negative Salmonella enterica serovar Typhimurium (S.e.s. Typhimurium). We found that Lo induced significant changes in the surface pressure isotherms of Langmuir monolayers representing the Salmonella enterica serovar Typhimurium inner membrane (S...
February 13, 2019: Scientific Reports
Eun Seon Chung, Kwan Soo Ko
Objectives: Persister cells following antibiotic exposure may cause failure of antibiotic treatment. The synergistic effects of antibiotic combinations with respect to eliminating persister cells were investigated based on their characteristics. Methods: For Acinetobacter baumannii clinical isolates, persister assays were performed using colistin, amikacin, imipenem and ciprofloxacin in various ways, including exposure to antibiotics in combination and sequentially...
February 12, 2019: Journal of Antimicrobial Chemotherapy
Zhou Ling, Chen Dayong, Yu Denggao, Wang Yiting, Fang Liaoqiong, Wang Zhibiao
BACKGROUND Recent studies have shown that Escherichia coli induced digestive tract diseases may be related to outer membrane vesicles (OMVs) induced intestinal double-strand breaks (DSBs) in intestinal epithelial cells. This study aimed to compare the impact of OMVs forces on DSBs in intestinal epithelial Caco-2 cells, and provide a new treatment for digestive diseases caused by E. coli. MATERIAL AND METHODS E.coli OMVs were prepared and co-cultured with Caco-2 cells. The uptake of OMVs by Caco-2 cells was observed by confocal microscopy...
February 11, 2019: Medical Science Monitor Basic Research
Pengfei Li, Andrew J Goodwin, James A Cook, Perry V Halushka, Xian K Zhang, Hongkuan Fan
Our previous data demonstrated that Friend leukemia virus integration 1 (Fli-1), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine sepsis by regulating essential pyroptosis markers including caspase-1. However, whether Fli-1 regulates caspase-1 expression levels in vitro and how Fli-1 regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed Fli-1 significantly increased caspase-1 and IL-18 expression levels in cultured mouse lung pericytes...
February 7, 2019: Molecular Immunology
Maurizio Ronci, Sonia Del Prete, Valentina Puca, Simone Carradori, Vincenzo Carginale, Raffaella Muraro, Gabriella Mincione, Antonio Aceto, Francesca Sisto, Claudiu T Supuran, Rossella Grande, Clemente Capasso
The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC belonging to the α- and β-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the α-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H...
December 2019: Journal of Enzyme Inhibition and Medicinal Chemistry
Outi L Lyytinen, Daria Starkova, Minna M Poranen
BACKGROUND: Cystoviruses have a phospholipid envelope around their nucleocapsid. Such a feature is unique among bacterial viruses (i.e., bacteriophages) and the mechanisms of virion envelopment within a bacterial host are largely unknown. The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. The phi6 major envelope protein P9 and the non-structural protein P12 are essential for the envelopment of its virions...
February 7, 2019: Microbial Cell Factories
Natalia Saiz-Ros, Rafal Czapiewski, Ilaria Epifano, Andrew Stevenson, Selene K Swanson, Charles R Dixon, Dario B Zamora, Marion McElwee, Swetha Vijayakrishnan, Christine A Richardson, Li Dong, David A Kelly, Lior Pytowski, Martin W Goldberg, Laurence Florens, Sheila V Graham, Eric C Schirmer
The primary envelopment/de-envelopment of Herpes viruses during nuclear exit is poorly understood. In Herpes simplex virus type-1 (HSV-1), proteins pUL31 and pUL34 are critical, while pUS3 and some others contribute; however, efficient membrane fusion may require additional host proteins. We postulated that vesicle fusion proteins present in the nuclear envelope might facilitate primary envelopment and/or de-envelopment fusion with the outer nuclear membrane. Indeed, a subpopulation of vesicle-associated membrane protein-associated protein B (VAPB), a known vesicle trafficking protein, was present in the nuclear membrane co-locating with pUL34...
February 3, 2019: Cells
Wei Zhang-Sun, François Tercé, Remy Burcelin, Alexey Novikov, Matteo Serino, Martine Caroff
The identification of a functional molecular moiety relating the lipopolysaccharides (LPSs) to their capacity to induce inflammation-mediated metabolic diseases needed to be performed. We previously described a proportional increase in the relative abundance of the 16SrDNA bacterial gene from the genus Ralstonia, within the microbiota from the adipose tissue stroma vascular fraction of obese patients, suggesting a causal role of the bacteria. Therefore, we first characterized the structures of the lipids A, the inflammatory inducing moieties of LPSs, of three Ralstonia species: Ralstonia eutropha, R...
January 28, 2019: Biochimie
Ying Wen, Shouwen Chen, Zhiwei Jiang, Zhuang Wang, Jinchao Tan, Tianjian Hu, Qiyao Wang, Xiangshan Zhou, Yuanxing Zhang, Qin Liu, Dahai Yang
Inflammasomes are important innate immune components in mammals. However, the bacterial factors modulating inflammasome activation in fish, and the mechanisms by which they alter fish immune defenses, remain to be investigated. In this work, a mutant of the fish pathogen Edwardsiella piscicida (E. piscicida), called 0909I, was shown to overexpress hemolysin, which could induce a robust pyroptotic-like cell death dependent on caspase-5-like-activity during infection in fish non-phagocyte cells. E. piscicida hemolysin was found to mainly associate with bacterial outer membrane vesicles (OMVs), which were internalized into the fish cells via a dynamin-dependent endocytosis and induced pyroptotic-like cell death...
January 31, 2019: Cellular Microbiology
Anja Pfalzgraff, Günther Weindl
Lipopolysaccharide (LPS) sensing in the cytosol by the noncanonical inflammasome leads to pyroptosis and NLRP3 inflammasome activation. This mechanism may be more critical for sepsis development than recognition of LPS by Toll-like receptor 4. LPS is directly binding to its intracellular receptor caspase-4/5/11, mediated by outer membrane vesicles and guanylate-binding proteins that deliver LPS to the cytosol and mediate access of caspases to LPS. Caspase-11-dependent cleavage of gasdermin D is discussed as a link between LPS-induced activation of caspases and pyroptosis or NLRP3 inflammasome activation...
January 25, 2019: Trends in Pharmacological Sciences
Anthony W Confer, Sahlu Ayalew
Mannheimia haemolytica is the major cause of severe pneumonia in bovine respiratory disease (BRD). Early M. haemolytica bacterins were either ineffective or even enhanced disease in vaccinated cattle, which led to studies of the bacterium's virulence factors and potential immunogens to determine ways to improve vaccines. Studies have focused on the capsule, lipopolysaccharide, various adhesins, extracellular enzymes, outer membrane proteins, and leukotoxin (LKT) resulting in a strong database for understanding immune responses to the bacterium and production of more efficacious vaccines...
December 2018: Animal Health Research Reviews
Christina Wodlej, Sabrina Riedl, Beate Rinner, Regina Leber, Carina Drechsler, Dennis R Voelker, Jae-Yeon Choi, Karl Lohner, Dagmar Zweytick
R-DIM-P-LF11-322 and DIM-LF11-318, derived from the cationic human host defense peptide lactoferricin show antitumor activity against human melanoma. While R-DIM-P-LF11-322 interacts specifically with cancer cells, the non-specific DIM-LF11-318 exhibits as well activity against non-neoplastic cells. Recently we have shown that cancer cells expose the negatively charged lipid phosphatidylserine (PS) in the outer leaflet of the plasma membrane, while non-cancer cells just expose zwitterionic or neutral lipids, such as phosphatidylcholine (PC) or cholesterol...
2019: PloS One
Katja Koeppen, Roxanna Barnaby, Angelyca A Jackson, Scott A Gerber, Deborah A Hogan, Bruce A Stanton
Tobramycin is commonly used to treat Pseudomonas aeruginosa lung infections in patients with Cystic Fibrosis (CF). Tobramycin treatment leads to increased lung function and fewer clinical exacerbations in CF patients, and modestly reduces the density of P. aeruginosa in the lungs. P. aeruginosa resides primarily in the mucus overlying lung epithelial cells and secretes outer membrane vesicles (OMVs) that diffuse through the mucus and fuse with airway epithelial cells, thus delivering virulence factors into the cytoplasm that modify the innate immune response...
2019: PloS One
Jessica D Cecil, Natalie Sirisaengtaksin, Neil M O'Brien-Simpson, Anne Marie Krachler
Outer membrane vesicles (OMVs) are nanosized proteoliposomes derived from the outer membrane of Gram-negative bacteria. They are ubiquitously produced both in culture and during infection and are now recognized to play crucial roles during host-microbe interactions. OMVs can transport a broad range of chemically diverse cargoes, including lipids and lipopolysaccharides, membrane-embedded and associated proteins and small molecules, peptidoglycan, and nucleic acids. Particularly, virulence factors such as adhesins and toxins are often enriched in OMVs...
January 2019: Microbiology Spectrum
Anshul Sinha, Sammy Nyongesa, Charles Viau, Samantha Gruenheid, Frédéric J Veyrier, Hervé Le Moual
Outer membrane vesicles (OMVs) are naturally produced by Gram-negative bacteria by a bulging of the outer membrane (OM) and subsequent release in the environment. By serving as vehicles for various cargos, including proteins, nucleic acids and small metabolites, OMVs are central to interbacterial interactions and both symbiotic and pathogenic host bacterial interactions. However, despite their importance, the mechanism of OMV formation remains unclear. Recent evidence indicates that covalent modifications of lipopolysaccharide (LPS) influence OMV biogenesis...
January 22, 2019: Journal of Bacteriology
Eduardo de la Torre-Escudero, Jared Q Gerlach, Adam P S Bennett, Krystyna Cwiklinski, Heather L Jewhurst, Kathryn M Huson, Lokesh Joshi, Michelle Kilcoyne, Sandra O'Neill, John P Dalton, Mark W Robinson
Helminth parasites secrete extracellular vesicles (EVs) that can be internalised by host immune cells resulting in modulation of host immunity. While the molecular cargo of EVs have been characterised in many parasites, little is known about the surface-exposed molecules that participate in ligand-receptor interactions with the host cell surface to initiate vesicle docking and subsequent internalisation. Using a membrane-impermeable biotin reagent to capture proteins displayed on the outer membrane surface of two EV sub-populations (termed 15k and 120k EVs) released by adult F...
January 18, 2019: PLoS Neglected Tropical Diseases
Hui-Ming Zhang, Luke B Devine, Xue Xia, Christina E Offler, John W Patrick
Transfer cells (TCs) facilitate high rates of nutrient transport into, and within, the plant body. Their transport function is conferred by polarized wall ingrowth papillae, deposited upon a specialized uniform wall layer, that form a scaffold supporting an amplified area of plasma membrane enriched in nutrient transporters. We explored the question of whether lipid-enriched domains of the TC plasma membrane could serve as organizational platforms for proteins regulating the construction of the intricate TC wall labyrinth using developing Vicia faba cotyledons...
January 14, 2019: Journal of Experimental Botany
Amit Garle, Bridgette Maria Budhlall
Novel polymers containing quaternary functional groups, with and without (control copolymer) PEG side chains were synthesized and characterized for their ability to lyse the phospholipid membranes of liposome vesicles. Calcein loaded unilamellar vesicles comprised of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) was used as a red-blood cell membrane mimic, and a 80:20 (mol/mol) mixture of 1,2-dioleoyl-sn-glycero-3-phosphatidyl ethanolamine (DOPE), and 1,2-dioleoyl-sn- glycero-3-[phospho-rac-(1-glycerol)] (DOPG), was used to mimic the outer cell-membrane of gram negative bacteria, E...
January 15, 2019: Langmuir: the ACS Journal of Surfaces and Colloids
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