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Chondrocytes septum nasal

Laura Lavernia, Wendy E Brown, Brian J F Wong, Jerry C Hu, Kyriacos A Athanasiou
Nasal cartilage pathologies are common; for example, up to 80% of people are afflicted by deviated nasal septum conditions. Because cartilage provides the supportive framework of the nose, afflicted patients suffer low quality of life. To correct pathologies, graft cartilage is often required. Grafts are currently sourced from the patient's septum, ear, or rib. However, their use yields donor site morbidity and is limited by tissue quantity and quality. Additionally, rhinoplasty revision rates exceed 15%, exacerbating the shortage of graft cartilage...
February 19, 2019: Acta Biomaterialia
Achim von Bomhard, Alexander Elsaesser, Ricarda Riepl, Katharina Pippich, Joseph Faust, Silke Schwarz, Ludwig Koerber, Roman Breiter, Nicole Rotter
Autologous cartilage as donor tissue for various surgical reconstructions such as nasal septum regeneration is limited and associated with donor site morbidity. Our goal was to evaluate a new resorbable chondroconductive biomaterial made of decellularized porcine nasal septum cartilage compared with autologous native auricular cartilage as the gold standard. In order to examine the material and determine its long-term outcome further, we used subcutaneous implantation and septal implantation in an orthotopic rabbit model...
January 25, 2019: Journal of Cranio-maxillo-facial Surgery
Hesham Aly Helal, Mohamed Abdel Mohsen Ghanem, Ahmed Mohamed Al-Badawy, Mohamed Mammdouh Abdel Haleem, Manal Hassan Mousa
Background: Rhinoplasty in the elderly requires different surgical approaches due to the morphological and structural changes affecting the nose by time. Objectives: In this study we aim to evaluate the age-related cellular and architectural changes of nasal cartilages and soft tissue attachments. Methods: This prospective study included 80 patients undergoing rhinoplasty. Patients were divided into 2 groups according to age. Group I included 40 patients with an age range of 19-39 years...
September 21, 2018: Aesthetic Surgery Journal
Ricardo de Souza Tesch, Esther Rieko Takamori, Karla Menezes, Rosana Bizon Vieira Carias, Cláudio Leonardo Milione Dutra, Marcelo de Freitas Aguiar, Tânia Salgado de Sousa Torraca, Alexandra Cristina Senegaglia, Cármen Lúcia Kuniyoshi Rebelatto, Debora Regina Daga, Paulo Roberto Slud Brofman, Radovan Borojevic
BACKGROUND: Upon orthognathic mandibular advancement surgery the adjacent soft tissues can displace the distal bone segment and increase the load on the temporomandibular joint causing loss of its integrity. Remodeling of the condyle and temporal fossa with destruction of condylar cartilage and subchondral bone leads to postsurgical condylar resorption, with arthralgia and functional limitations. Patients with severe lesions are refractory to conservative treatments, leading to more invasive therapies that range from simple arthrocentesis to open surgery and prosthesis...
April 7, 2018: Stem Cell Research & Therapy
Yong Wan, Matthew B Rogers, Heather L Szabo-Rogers
The nose is the central feature of the amniote face. In adults, the nose is a structurally and functionally complex organ that consists of bone, cartilage, glands and ducts. In an ongoing expression screen in our lab, we found several novel markers for specific tissues in the nasal region. Here, using in situ hybridization expression experiments, we report that Alx1, Ap-2β, Crispld1, Eya4, Moxd1, and Penk have tissue specific expression during murine nasal development. At E11.5, we observed that Alx1, Ap-2β, Crispld1, and Eya4 are expressed in the medial and lateral nasal prominences...
January 2018: Gene Expression Patterns: GEP
Wenliang Chen, Changhua Li, Maoxiu Peng, Bingju Xie, Lei Zhang, Xiaojun Tang
Cell based tissue engineering serves as a promising strategy for articular cartilage repair, which remains a challenge both for researchers and clinicians. The aim of this research was to assess the potential of autologous nasal chondrocytes (NCs) combined with alginate hydrogel as injectable constructs for rabbit articular cartilage repair. Autologous nasal chondrocytes were isolated from rabbit nasal septum, expanded either on monolayer or in 3D alginate hydrogel. In vitro, DNA quantification revealed that NCs can proliferate stable in 3D alginate matrix, but slower than that cultured in monolayer...
March 2018: Cell and Tissue Banking
Karoliina Pelttari, Marcus Mumme, Andrea Barbero, Ivan Martin
Cells deriving from neural crest are generally acknowledged during embryonic development for their multipotency and plasticity, accounting for their capacity to generate various cell and tissue types even across germ layers. At least partial preservation of some of these properties in adulthood makes neural crest derived cells of large interest for regenerative purposes. Chondrocytes from fully mature nasal septum cartilage in adults are also derivatives of neural crest cells and were recently demonstrated to be able not only to maintain functionality across serial cloning, as surrogate self-renewal test, but also to respond and adapt to heterotopic transplantation sites...
October 2017: Current Opinion in Biotechnology
Juliana Marulanda, Hazem Eimar, Marc D McKee, Michelle Berkvens, Valentin Nelea, Hassem Roman, Teresa Borrás, Faleh Tamimi, Mathieu Ferron, Monzur Murshed
Genetic and environmental factors may lead to abnormal growth of the orofacial skeleton, affecting the overall structure of the face. In this study, we investigated the craniofacial abnormalities in a mouse model for Keutel syndrome, a rare genetic disease caused by loss-of-function mutations in the matrix Gla protein ( MGP ) gene. Keutel syndrome patients show diffuse ectopic calcification of cartilaginous tissues and impaired midface development. Our comparative cephalometric analyses of micro-computed tomography images revealed a severe midface hypoplasia in Mgp -/- mice...
July 7, 2017: Journal of Biological Chemistry
Srujana Vedicherla, Conor Timothy Buckley
Laboratory based processing and expansion to yield adequate cell numbers had been the standard in Autologous Disc Chondrocyte Transplantation (ADCT), Allogeneic Juvenile Chondrocyte Implantation (NuQu®), and Matrix-Induced Autologous Chondrocyte Implantation (MACI). Optimizing cell isolation is a key challenge in terms of obtaining adequate cell numbers while maintaining a vibrant cell population capable of subsequent proliferation and matrix elaboration. However, typical cell yields from a cartilage digest are highly variable between donors and based on user competency...
2017: BioMed Research International
Loraine L Y Chiu, William T H To, John M Lee, Stephen D Waldman
OBJECTIVE: Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demonstrate the application of the bioreactor to generate large-sized tissues from a small population of primary human nasoseptal chondrocytes...
March 2017: Laryngoscope
Pietro Gentile, Maria G Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
BACKGROUND: Developing cartilage constructs with injectability, appropriate matrix composition, and persistent cartilaginous phenotype remains an enduring challenge in cartilage repair. The combined use of autologous chondrocyte micrografts and platelet-rich plasma (PRP) is an alternative that opens a new era in this field. METHODS: At the Department of Plastic and Reconstructive Surgery, University of Rome Tor Vergata, Italy, 11 patients underwent nasal alar reconstruction with chondrocyte micrografts gently poured onto PRP in solid form...
October 2016: Plastic and Reconstructive Surgery. Global Open
Marcus Mumme, Andrea Barbero, Sylvie Miot, Anke Wixmerten, Sandra Feliciano, Francine Wolf, Adelaide M Asnaghi, Daniel Baumhoer, Oliver Bieri, Martin Kretzschmar, Geert Pagenstert, Martin Haug, Dirk J Schaefer, Ivan Martin, Marcel Jakob
BACKGROUND: Articular cartilage injuries have poor repair capacity, leading to progressive joint damage, and cannot be restored predictably by either conventional treatments or advanced therapies based on implantation of articular chondrocytes. Compared with articular chondrocytes, chondrocytes derived from the nasal septum have superior and more reproducible capacity to generate hyaline-like cartilage tissues, with the plasticity to adapt to a joint environment. We aimed to assess whether engineered autologous nasal chondrocyte-based cartilage grafts allow safe and functional restoration of knee cartilage defects...
October 22, 2016: Lancet
Katie Bardsley, Agnieska Kwarciak, Christine Freeman, Ian Brook, Paul Hatton, Aileen Crawford
The regeneration of large bone defects remains clinically challenging. The aim of our study was to use a rat model to use nasal chondrocytes to engineer a hypertrophic cartilage tissue which could be remodelled into bone in vivo by endochondral ossification. Primary adult rat nasal chondrocytes were isolated from the nasal septum, the cell numbers expanded in monolayer culture and the cells cultured in vitro on polyglycolic acid scaffolds in chondrogenic medium for culture periods of 5-10 weeks. Hypertrophic differentiation was assessed by determining the temporal expression of key marker genes and proteins involved in hypertrophic cartilage formation...
January 2017: Biomaterials
A F Elsaesser, S Schwarz, H Joos, L Koerber, R E Brenner, N Rotter
BACKGROUND: Progenitor cells display interesting features for tissue repair and reconstruction. In the last years, such cells have been identified in different cartilage types. In this study, we isolated a migrative subpopulation of adult human nasoseptal chondrocytes with progenitor cell features by outgrowth from human nasal septum cartilage. These putative progenitor cells were comparatively characterized with mesenchymal stem cells (MSC) and human nasal septum chondrocytes with respect to their cellular characteristics as well as surface marker profile using flow cytometric analyses...
2016: Cell & Bioscience
Abbas Shafiee, Mahboubeh Kabiri, Lida Langroudi, Masoud Soleimani, Jafar Ai
Cell-based therapy is being considered as a promising approach to regenerate damaged cartilage. Though, autologous chondrocyte implantation is the most effective strategy currently in use, but is hampered by some drawbacks seeking comprehensive research to surmount existing limitations or introducing alternative cell sources. In this study, we aimed to evaluate and compare the in vitro characteristics and chondrogenic capacity of some easily available adult cell sources for use in cartilage repair which includes: bone marrow-derived mesenchymal stem cells (MSC), adipose tissue-derived MSC, articular chondrocyte progenitors, and nasal septum-derived progenitors...
March 2016: Journal of Biomedical Materials Research. Part A
M Rita Correro-Shahgaldian, Chafik Ghayor, Nicholas D Spencer, Franz E Weber, Luigi M Gallo
Mechanical stress is listed as a main risk factor for cartilage degradation. The aim of this study was to investigate the biological response of cartilage to dynamic loading such as plowing. Cartilage strips harvested from bovine nasal septum were submitted to plowing using a cylindrical indenter, applying a constant normal force in the vertical axis and moving at constant speed in the horizontal axis. After plowing, cell viability, gene expression and glycosaminoglycan (GAG) release were measured with conventional assays...
2014: Cells, Tissues, Organs
Benjamin E Pippenger, Manuela Ventura, Karoliina Pelttari, Sandra Feliciano, Claude Jaquiery, Arnaud Scherberich, X Frank Walboomers, Andrea Barbero, Ivan Martin
Nasal chondrocytes (NC) derive from the same multipotent embryological segment that gives rise to the majority of the maxillofacial bone and have been reported to differentiate into osteoblast-like cells in vitro. In this study, we assessed the capacity of adult human NC, appropriately primed towards hypertrophic or osteoblastic differentiation, to form bone tissue in vivo. Hypertrophic induction of NC-based micromass pellets formed mineralized cartilaginous tissues rich in type X collagen, but upon implantation into subcutaneous pockets of nude mice remained avascular and reverted to stable hyaline-cartilage...
June 2015: Journal of Cellular and Molecular Medicine
Ayman A Al Dayeh, Susan W Herring
The growth of the nasal septal cartilage is believed to be a driving force of midfacial growth. Cellular proliferation is an important contributor to growth of the cartilage, but this factor has been rarely investigated. The current study was undertaken to assess the proliferation and cellular density in the septal cartilage of fast-growing juvenile minipigs. Six minipigs averaging 4.4 ± 1 months old were injected with 5'-bromo-2'-deoxyuridine (BrdU), a thymidine analog, 24 h before death. The septal cartilage was sectioned in the coronal plane and reacted for BrdU...
December 2014: Journal of Anatomy
I Tsukamoto, M Akagi, S Inoue, K Yamagishi, S Mori, S Asada
In 2013, we reported that local renin-angiotensin system (local RAS) components express during the hypertrophic differentiation of chondrocytes and can modulate it, using ATDC5 cell line that involves differentiation from mesenchymal stem cells to calcified hypertrophic chondrocytes. However, the expressions of local RAS components in normal chondrocytes have not been revealed yet. The purpose of this study is to examine the expression of the local RAS components in chondrocytes in vivo and the conditions allowing the expression...
2014: European Journal of Histochemistry: EJH
Katharina Stoelzel, Benjamin Kohl, Mariann Hoyer, Carola Meier, Agnieszka J Szczepek, Heidi Olze, Gundula Schulze-Tanzil
Nasal sprays were introduced several years ago to support the treatment of allergic rhinitis. These sprays may come in direct contact with directly exposed nasoseptal cartilage (e.g. is case of nasoseptal perforation). To date, no studies investigated the effects of nasal sprays on cartilage tissues and cells. Therefore, our aim was to analyze the influence of two different nasal spray types (thixotropic and liposomal) on the vitality of nasoseptal chondrocytes. Human chondrocytes were isolated from surgically dissected tissues...
April 2015: European Archives of Oto-rhino-laryngology
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