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Xiao-Chen Ma, Si-Yu Zhu, Miao-Miao Luo, Xue-Chao Hu, Cheng Peng, He Huang, Lu-Jing Ren
Menaquinone-7 (MK-7) plays an important role in blood clotting, cardiovascular disease and anti-osteoporosis, and has been wildly used in the food additives and pharmaceutical industries. The aim of this study was to investigate the mechanism of menaquinone-7 biosynthesis in response to different oxygen supplies in Bacillus natto. The differences of fermentation performance, intracellular metabolites, oxidative stress reaction and enzyme activities of Bacillus natto R127 were analyzed under different KLa. Glycerol consumption rate and MK-7 yield at 24...
February 13, 2019: Bioprocess and Biosystems Engineering
Chih-Yung Chiu, Mei-Ling Cheng, Kin-Sun Wong, Shen-Hao Lai, Meng Han Chiang, Ming-Han Tsai, Gigin Lin
Fibrin formation in infectious parapneumonic effusion (IPE) characterizes complicated parapneumonic effusion and is important for providing guidelines for management of IPEs that require aggressive interventions. We aim to identify metabolic mechanisms associated with bacterial invasion, inflammatory cytokines, and biochemical markers in cases of fibrinous infectious pleural effusions in children with pneumonia. Pleural fluid metabolites were determined by 1H-nuclear magnetic resonance (NMR) spectroscopy. Metabolites contributed for the separation between fibrinous and non-fibrinous IPEs were identified using supervised partial least squares-discriminant analysis (Q2/R2 = 0...
February 13, 2019: Journal of Proteome Research
Oleksandr Anatolievich Volodchenko, Leonid Vladimirovich Podrigalo, Sergii Sidorovich Iermakov, Małgorzata Teresa Żychowska, Władysław Jagiełło
The study aimed to determine the suitability of testing the saliva of kickboxing athletes to show changes in biochemical parameters in dynamic of training. 8 elite male athletes (mean age 17.29± 0.31 years, body mass 66.82± 3.46kg, with 5.62±0.96 years of training experience) participated in the study. Indicators of lipid peroxidation and glycolysis (the concentration of lactic acid and pyruvic acid) were defined before and after a training session. Significant increases in indicators of lipid peroxidation activity indicators and the concentration of lactic acid (4-fold) were observed; analysis of correlation matrices confirms the absence of expressed changes...
2019: BioMed Research International
Yurika Katsu-Jiménez, Carmela Vázquez-Calvo, Camilla Maffezzini, Maria Halldin, Xiaoxiao Peng, Christoph Freyer, Anna Wredenberg, Alfredo Giménez-Cassina, Anna Wedell, Elias S J Arnér
Thioredoxin Interacting Protein (TXNIP) is an alpha arrestin that can bind to and inhibit the antioxidant protein thioredoxin (TXN). TXNIP expression is induced by glucose, promotes ß-cell apoptosis in the pancreas and deletion of its gene in mouse models protects against diabetes. TXNIP is currently studied as a potential new target for antidiabetic drug therapy. Here we describe a family with a mutation in the TXNIP gene leading to non-detectable expression of TXNIP protein. Symptoms of affected family members include lactic acidosis and low serum methionine levels...
February 12, 2019: Diabetes
Shuping Du, Yuanyuan Zhang, Jing Yang, Xiaoyan Liu, Yuqing Wang, Bo Xu, Ji Jia
Curcumin protects neuronal cells exposed to β amyloid (Aβ); the mechanism, however, is still obscure. The aim of this study is to determine whether the type 2 superoxide dismutase (SOD2) mediates curcumin-induced protective effects in Aβ-treated neuronal cells. In this study, the HT22 neuronal cells were exposed to Aβ to imitate neuronal injury in Alzheimer's disease (AD). After 24-h treatment, 10 μM Aβ decreased cell viability and mitochondrial functions, including mitochondrial complex activities and mitochondrial membrane potential (MMP), and also downregulated anti-oxidants SOD2, glutathione (GSH), and catalase (CAT) levels (P < 0...
February 12, 2019: Journal of Molecular Neuroscience: MN
Ingrid M Quintana, Johannes Gibhardt, Asan Turdiev, Elke Hammer, Fabian M Commichau, Vincent T Lee, Christian Magni, Jörg Stülke
Cyclic di-adenosine monophosphate (c-di-AMP) is a second messenger involved in diverse metabolic processes including osmolyte uptake, cell wall homeostasis, as well as antibiotic and heat resistance. In Lactococcus lactis , a lactic acid bacterium which is used in the dairy industry and as a cell factory in biotechnological processes, the only reported interaction partners of c-di-AMP are the pyruvate carboxylase and BusR, the transcription regulator of the busAB operon for glycine betaine uptake. However, recent studies uncovered a major role of c-di-AMP in the control of potassium homeostasis, and potassium as the signal that triggers c-di-AMP synthesis...
February 11, 2019: Journal of Bacteriology
Leandro Janke, Bernadette Kathleen McCabe, Peter Harris, Andrew Hill, Seonmi Lee, Sören Weinrich, Serhiy Marchuk, Craig Baillie
Ensiling of sugarcane trash (SCT) and sugarcane stalks (SCS) was studied to assess the effects of molasses (MOL) and lactic acid bacteria (LAB) inoculant on methane potential. The experiment was run for 70 days and monitoring parameters were analyzed at days 0, 5, 15 and 70. Biochemical methane potential (BMP) tests performed with fresh and ensiled material at day 70 showed an increase in methane potential by 24.0%, 23.4%, 1.7% and 71.1% for SCSctr , SCTctr , SCTmol and SCTmol + lab , respectively. Such improved performance is explained by the formation of organic acids (mostly acetate and lactate) which were able to decrease the pH of the silages from 5...
January 31, 2019: Bioresource Technology
Susumu Muroya, Mika Oe, Koichi Ojima, Akira Watanabe
Objective: Meat quality attributes in postmortem muscle tissues depend on skeletal muscle metabolites. The objective of this study was to determine the key metabolic compounds and pathways associated with postmortem aging and beef quality in Japanese Black cattle (JB; a Japanese Wagyu breed with highly marbled beef). Methods: Lean portions of Longissimus thoracis (LT: loin) muscle in 3 JB steers were collected at 0, 1, and 14 days after slaughter. The metabolomic profiles of the samples were analyzed by capillary electrophoresis time-of-flight mass spectrometry, followed by statistical and multivariate analyses with bioinformatics resources...
January 4, 2019: Asian-Australasian Journal of Animal Sciences
Pussadee Tangwatcharin, Jiraroj Nithisantawakhup, Supaluk Sorapukdee
Objective: The aims of this study were to select one strain of Lactobacillus platarum for a potential indigenous safe starter culture with low level antibiotic resistant and low biogenic amine production and evaluate its effect on biogenic amines reduction in Moo som. Methods: Three strains of indigenous L. plantarum starter cultures (KL101, KL102 and KL103) were selected on the basis of their safety including antibiotic resistance and decarboxylase activity, and fermentation property as compared with a commercial starter culture (L...
February 7, 2019: Asian-Australasian Journal of Animal Sciences
Katarzyna Pielech-Przybylska, Maria Balcerek, Urszula Dziekońska-Kubczak, Barbara Pacholczyk-Sienicka, Grzegorz Ciepielowski, Łukasz Albrecht, Piotr Patelski
This study set out to assess the acetone content in rye sweet mashes prepared using the thermal-pressure method of starch liberation, and to investigate the formation of 2-propanol during the fermentation process. In the first set of experiments, we evaluated the correlation between the color and the content of acetone and furfural in industrially produced sweet mashes ( n = 37). The L * value was negatively correlated with the content of both acetone and furfural, while chromatic parameters a * and b * and the yellowness index (YI) had strong positive correlations with acetone (r > 0...
February 9, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Dan Zhang, Yiming Li, Yinuo Gao, Mbage Bawa, Mingxin Huo, Xiaohong Wang, Suiyi Zhu
A novel route was created to facilitate the degradation of diethyl phthalate (DEP) upon micellar polyoxometalate (POM) catalysts and H2 O2 . The best catalytic activity was obtained using [C16 H33 N(CH3 )3 ]H4 PMo10 V2 O40 (N-hexadecyl-N,N,N-trimethylammonium tetrahydrogen decamolybdo-divanadophosphate, abbreviated as (CTA)H4 PMoV) with 90.2% degradation efficiency within 30 min, while the chemical oxygen demand (COD) and total organic carbon (TOC) removal efficiency were about 77.7% and 74.3% within 40 min...
February 1, 2019: Journal of Hazardous Materials
Peijun Tian, Gang Wang, Jianxin Zhao, Hao Zhang, Wei Chen
Depression disorder is rapidly advancing worldwide, and therapeutic strategy through gut-brain axis has been proven to be effective in the treatment. Here we studied the effect of lactic acid bacteria (LAB) treatment on depression. C57BL/6J mice were administered with LAB during a 5-week chronic unpredictable mild stress. Bifidobacterium longum subsp. infantis E41 and Bifidobacterium breve M2CF22M7, which improved the expression of Tph1 and secretion of 5-hydroxytryptophan (5-HTP) in RIN14B cells, significantly reduced depressive behaviors of mice in the forced swim test, sucrose preference test and step-down test, as well as increased the level of 5-hydroxytryptamine and brain-derived neurotrophic factor concentration in brain...
January 18, 2019: Journal of Nutritional Biochemistry
Aki Inoue, Shinichi Iwasaki, Chisato Fujimoto, Makoto Kinoshita, Tatsuya Yamasoba
OBJECTIVE: To evaluate the progression of peripheral vestibular dysfunction in patients with an A-to-G point mutation at nucleotide pair 3243 in mitochondrial DNA (A3243G mutation). STUDY DESIGN: Retrospective patient series. SETTING: Tertiary referral center. PATIENTS: Six unrelated patients with an A3243G mutation (four mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes; and two maternally inherited diabetes and deafness; five females and one male; average age 41 ± 11...
March 2019: Otology & Neurotology
Krista Bohnert, Andrew Dorizas, Paul Lorenc, Neil S Sadick
BACKGROUND: Poly-L-lactic acid (PLLA) is an injectable filler used for restoring facial fat volume loss. OBJECTIVE: To evaluate the effect of repeated PLLA injections on skin quality. METHODS: Forty healthy women were enrolled in this randomized, controlled, double-blind, multicenter study. Eligible subjects received 3 treatments every 4 weeks with either PLLA (treatment group) or saline (control group) injections, into both sides of the face...
February 6, 2019: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Mari Kuwajima, Masahide Goto, Koyuru Kurane, Hiroko Shimbo, Narumi Omika, Eriko F Jimbo, Kazuhiro Muramatsu, Makiko Tajika, Masaru Shimura, Kei Murayama, Kenji Kurosawa, Takanori Yamagata, Hitoshi Osaka
Mutations in the mitochondrial tRNAMet gene have been reported in only five patients to date, all of whom presented with muscle weakness and exercise intolerance as signs of myopathy. We herein report the case of a 12-year-old girl with focal epilepsy since the age of eight years. At age 11, the patient developed sudden visual disturbances and headaches accompanied by recurrent, stroke-like episodes with lactic acidosis (pH 7.279, lactic acid 11.6 mmol/L). The patient frequently developed a delirious state, exhibited regression of intellectual ability...
February 7, 2019: Brain & Development
Gobinath Chandrakasan, Adriana-Inés Rodríguez-Hernández, Ma Del Rocío López-Cuellar, Heidi-María Palma-Rodríguez, Norberto Chavarría-Hernández
The encapsulation of bacteriocins from lactic acid bacteria has involved several methods to protect them from unfavourable environmental conditions and incompatibilities. This review encompasses different methods for the encapsulation of bacteriocins and their applications in both food and pharmaceutical fields. Based on the bibliometric analysis of publications from well-reputed journals including different available patents during the period from 1996 to 2017, 135 articles and 60 patents were collected. Continent-wise contributions to the bacteriocins encapsulation research were carried out by America (52%), Asia (29%) and Europe (19%); with the United States of America, Brazil, Thailand and Italy the countries with major contributions...
February 9, 2019: Biotechnology Letters
Ufuk Bagci, Sine Ozmen Togay, Ayhan Temiz, Mustafa Ay
As potential probiotic traits of human milk-isolated bacteria have increasingly been recognized, this study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum. Among 118 human milk- and colostrum-isolated lactic cocci, only 29 were identified as Enterococcus. Of these, only four Enterococcus faecium isolates exhibited bacteriocigenic activity against several pathogenic Gram-positive bacteria, including Listeria monocytogenes...
February 9, 2019: Folia Microbiologica
Chang-Jiang Lyu, Lu Liu, Jun Huang, Wei-Rui Zhao, Sheng Hu, Le-He Mei, Shan-Jing Yao
γ-Aminobutyrate (GABA) is an important chemical in pharmaceutical field. The use of lactic acid bacteria as biocatalysts for the conversion of l-monosodium glutamate (MSG) into GABA opens interesting perspectives for the production of this functional compound. In this work, an engineered GABA high-producing strain Lactobacillus brevis GadAΔC14 was constructed by overexpressing a C-terminally truncated GadA mutant, which is active in expanded pH range. After comparison with agar and κ-carrageenan, gellan gum was selected as the optimal immobilization support, and the properties of L...
February 7, 2019: Journal of Bioscience and Bioengineering
Magdalena Espino, Manuel Solari, María de Los Ángeles Fernández, Joana Boiteux, María Roxana Gómez, María Fernanda Silva
Candida albicans is an opportunistic pathogenic yeast commonly found in mouth, gastrointestinal tract and vagina. Under certain conditions, it causes skin, mucosal and systemic infections. With growing concern over the emergence of resistant strains to conventional antifungals, the development of novel antifungal agents for the management of this pathogen is an urgent need. In the present work, novel bioextracts from folk medicinal plants were directly used as active ingredient in a topical formulation for dermal candidiasis...
January 30, 2019: Journal of Pharmaceutical and Biomedical Analysis
Yasutoshi Koga, Nataliya Povalko, Eisuke Inoue, Kazutaka Nashiki, Masashi Tanaka
Biomarkers and two clinical rating scales-the Japanese mitochondrial disease-rating scale (JMDRS) and Newcastle mitochondrial disease adult scale (NMDAS)-are clinically used when treating patients with mitochondrial disease. We explored the biomarker(s) and clinical rating scale(s) that are appropriate in preparing the protocol for a future clinical trial of sodium pyruvate (SP) therapy. A 48-week, prospective, single-centre, exploratory, clinical study enrolled 11 Japanese adult patients with genetically, biochemically, and clinically confirmed mitochondrial disease; they had intractable lactic acidosis and received SP (0...
February 6, 2019: Mitochondrion
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