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three-dimensional culture

Yamin Yang, Chansoo Choi, Guorong Xie, Jong-Deok Park, Shao Ke, Jong-Sung Yu, Juanjuan Zhou, Bongsu Lim
Biofilm-coated electrodes and outer cell membrane-mimicked electrodes were examined to verify an extracellular electron transfer mechanism using Marcus theory for a donor-acceptor electron transfer. Redox couple-bound membrane electrodes were prepared by impregnating redox coenzymes into Nafion films on carbon cloth electrodes. The electron transfer was believed to occur sequentially from acetate to nicotinamide adenine dinucleotide (NAD), c-type cytochrome, flavin mononucleotide (FMN) (or riboflavin (RBF)) and the anode substrate...
February 8, 2019: Bioelectrochemistry
Silvia Codenotti, Fiorella Faggi, Roberto Ronca, Paola Chiodelli, Elisabetta Grillo, Michele Guescini, Francesca Megiorni, Francesco Marampon, Alessandro Fanzani
Rhabdomyosarcoma (RMS) is a pediatric soft tissue tumor classified in two major subtypes namely embryonal and alveolar, which have distinctive histopathological and genetic signatures and worse outcomes in the presence of metastases. Here, in order to evaluate the role of Caveolin-1 (Cav-1) in embryonal RMS dissemination, we employed an experimental in vivo metastasis assay using immunodeficient NOD/SCID mice. We found that the intravenous injection of human RD cells engineered for Cav-1 overexpression promoted the formation of lung metastases compared to parental cells...
February 13, 2019: Cancer Letters
Hongbo Tang, Johanna F A Husch, Yang Zhang, John A Jansen, Fang Yang, Jeroen J J P van den Beucken
The effects of immune cells, in particular macrophages, on the behavior of mesenchymal stem cells (MSCs) have recently gained much attention for MSCs-based tissue-engineered constructs. This study aimed to evaluate the effect of monocytes/macrophages on the osteogenic differentiation of adipose-derived mesenchymal stromal cells (ADMSCs) in three-dimensional (3D) co-cultures. For this, we co-cultured THP-1 monocytes, M1 macrophages or M2 macrophages with ADMSCs on 3D poly (lactic-co-glycolic) acid (PLGA)/polycaprolactone (PCL) scaffolds using osteogenic medium for up to 42 days...
February 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
Cara L Croft, Pedro E Cruz, Daniel H Ryu, Carolina Ceballos-Diaz, Kevin H Strang, Brittany M Woody, Wen-Lang Lin, Michael Deture, Edgardo Rodríguez-Lebrón, Dennis W Dickson, Paramita Chakrabarty, Yona Levites, Benoit I Giasson, Todd E Golde
It has been challenging to produce ex vivo models of the inclusion pathologies that are hallmark pathologies of many neurodegenerative diseases. Using three-dimensional mouse brain slice cultures (BSCs), we have developed a paradigm that rapidly and robustly recapitulates mature neurofibrillary inclusion and Lewy body formation found in Alzheimer's and Parkinson's disease, respectively. This was achieved by transducing the BSCs with recombinant adeno-associated viruses (rAAVs) that express α-synuclein or variants of tau...
February 15, 2019: Journal of Experimental Medicine
Yi Zhao, Ranjith Kumar Kankala, Shi-Bin Wang, Ai-Zheng Chen
With advantageous features such as minimizing the cost, time, and sample size requirements, organ-on-a-chip (OOC) systems have garnered enormous interest from researchers for their ability for real-time monitoring of physical parameters by mimicking the in vivo microenvironment and the precise responses of xenobiotics, i.e., drug efficacy and toxicity over conventional two-dimensional (2D) and three-dimensional (3D) cell cultures, as well as animal models. Recent advancements of OOC systems have evidenced the fabrication of 'multi-organ-on-chip' (MOC) models, which connect separated organ chambers together to resemble an ideal pharmacokinetic and pharmacodynamic (PK-PD) model for monitoring the complex interactions between multiple organs and the resultant dynamic responses of multiple organs to pharmaceutical compounds...
February 14, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Laura Nehrenheim, Silja Raschke, Anja Stefanski, Mareike Barth, Jessica Isabel Selig, Andreas Barbian, Alicia Fernández-Colino, Kai Stühler, Petra Mela, Alexander Albert, Artur Lichtenberg, Payam Akhyari
 Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood. Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments. 
 Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves...
February 15, 2019: Biomedical Materials
Jarel K Gandhi, Travis Knudsen, Matthew Hill, Bhaskar Roy, Lori Bachman, Cynthia Pfannkoch-Andrews, Karina N Schmidt, Muriel M Metko, Michael J Ackerman, Zachary Resch, Jose S Pulido, Alan D Marmorstein
Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e.g., laminin) for expansion in adherent cell culture for use in cell therapy. To address this, we investigated the potential of culturing iPSCs on fibrin hydrogels for three-dimensional applications and further examined the use of fibrinogen, the soluble precursor protein, as a coating substrate for traditional adherent cell culture...
February 15, 2019: Stem Cells Translational Medicine
Pan Xu, Elhaseen Elamin, Montserrat Elizalde, Paul P H A Bours, Marieke J Pierik, Ad A M Masclee, Daisy M A E Jonkers
Intestinal epithelial barrier is affected by multiple factors, such as tumour necrosis factor-α (TNF-α). Plasma concentration of TNF-α is higher in patients with Crohn's disease (CD) than healthy controls (HC) and correlates positively with disease activity. This study aimed to determine the effect of plasma from active, inactive CD patients on intestinal barrier function and to investigate the underlying mechanism. Plasma samples were collected from CD patients and HC. 3D Caco-2 cysts were treated with plasma or TNF-α, with or without pre-incubation of adalimumab (a monoclonal antibody that antagonizes TNF-α) or JNK inhibitor SP600125...
February 14, 2019: Scientific Reports
Ignacio Albert-Smet, Asier Marcos-Vidal, Juan José Vaquero, Manuel Desco, Arrate Muñoz-Barrutia, Jorge Ripoll
Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the millimeter scale). Such microscopes distinguish themselves for having very low phototoxicity levels and high spatial and temporal resolution, properties that make them ideal for a large range of applications. These include the study of cellular dynamics, in particular cellular motion which is essential to processes such as tumor metastasis and tissue development...
2019: Frontiers in Neuroanatomy
Kolene E Bailey, Tyler J D'Ovidio, Nicole Manning, Melanie Koenigshoff, Chelsea M Magin
RATIONALE: Continued reliance on preclinical animal models and traditional human cell culture experiments conducted on supraphysiologically stiff surfaces in two dimensions often limits the translation of cellular and molecular mechanistic findings into effective therapies for patients suffering from chronic obstructive pulmonary disease (COPD). COPD ranks as the fourth highest cause of mortality in the United States, and thus it is imperative to improve ex vivo models of this devastating disease...
December 2018: Annals of the American Thoracic Society
Kolin C Hribar, Christopher J Wheeler, Alexey Bazarov, Kunal Varshneya, Ryosuke Yamada, Padraig Buckley, Chirag G Patil
A cell culture platform that enables ex vivo tissue growth from patients or patient-derived xenograft (PDX) models and assesses sensitivity to approved therapies (e.g. Temozolomide, TMZ) in a clinically relevant timeframe would be very useful in translational research and personalized medicine. Here, we present a novel three-dimensional (3D) ECM hydrogel system, VersaGel, for assaying ex vivo growth and therapeutic response with standard image microscopy. Specifically, multicellular spheroids deriving from either five glioblastoma (GBM) patients or a renal cell carcinoma (RCC) PDX model were incorporated into VersaGel and treated with TMZ and several other therapies, guided by the most recent advances in GBM treatment...
February 12, 2019: Molecular Cancer Therapeutics
Mirelis Santos-Cancel, Laura Walker Simpson, Jennie B Leach, Ryan J White
In this manuscript, we describe the development and application of electrochemical, aptamer-based (E-AB) sensors directly interfaced with astrocytes in three-dimensional (3D) cell culture to monitor stimulated release of adenosine triphosphate (ATP). The aptamer-based sensor couples specific detection of ATP, selective performance directly in cell culture media, and seconds time resolution using squarewave voltammetry for quantitative ATP-release measurements. More specifically, we demonstrate the ability to quantitatively monitor ATP release into the extracellular environment after stimulation by the addition of calcium (Ca2+), ionomycin, and glutamate...
February 12, 2019: ACS Chemical Neuroscience
Fabrizio Fontana, Michela Raimondi, Monica Marzagalli, Michele Sommariva, Patrizia Limonta, Nicoletta Gagliano
Three-dimensional (3D) cell cultures allow the mimic of functions of living tissues andprovide key information encoded in tissue architecture. Considered the pivotal role of epithelial-tomesenchymaltransition (EMT) in carcinoma progression, including prostate cancer (PCa), weaimed at investigating the effect of the 3D arrangement on the expression of some key markers ofEMT in cultured human prostate cancer (PCa) cells, to better understand PCa cell behavior. PC3 andDU145 PCa cells were cultured in RPMI cell culture medium either in 2D-monolayers or in 3Dspheroids...
February 11, 2019: Cells
Diego Marescotti, Tommaso Serchi, Karsta Luettich, Yang Xiang, Elisa Moschini, Marja Talikka, Florian Martin, Karine Baumer, Remi Dulize, Dariusz Peric, David Bornand, Emmanuel Guedj, Alain Sewer, Sebastian Cambier, Servane Contal, Aline Chary, Arno C Gutleb, Stefan Frentzel, Nikoloai V Ivanov, Manuel C Peitsch, Julia Hoeng
To more accurately model inhalation toxicity in vitro, we developed a tetra-culture system that combines lung alveolar epithelial cells, endothelial cells, macrophages, and mast cells in a three-dimensional orientation. We characterized the influence of the added complexity using network perturbation analysis and gene expression data. This will allow us to gain insight into the steady-state profile of the assembled, complete three-dimensional model using all four cell types and of simpler models of one, two, or three cell types...
February 12, 2019: ALTEX
Hirotoshi Miyoshi, Chiaki Sato, Yuichiro Shimizu, Misa Morita
With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2 weeks of coculture in a serum-containing medium without the addition of cytokines...
February 12, 2019: International Journal of Artificial Organs
Theresa Chow, Jennifer Whiteley, Ian M Rogers
Decellularization is the process by which resident cells are lysed and cellular debris is removed from the tissue, leaving behind the extracellular matrix scaffold. The extracellular matrix scaffold can be used for three-dimensional culturing of cells. Here we describe methods of decellularizing whole and thick sections of mouse kidneys using a 0.1% sodium dodecyl sulfate (SDS) detergent solution and strategies to repopulate whole and thick sections of decellularized mouse kidneys with cells.
2019: Methods in Molecular Biology
Ulla Saarela, Ilya Skovorodkin
Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions-3D and the time.
2019: Methods in Molecular Biology
Kai Zhang, Aki Manninen
Cells in tissues in vivo face a very different microenvironment than typical cultured cells plated on a plastic dish. Already several decades ago, cell biologists observed that cell lines show dramatically different morphology and growth characteristics when embedded into three-dimensional (3D) substrates or standard tissue culture plates (Montesano R, Schaller G, Orci L, Cell. 66:697-711, 1991; Barcellos-Hoff MH, Aggeler J, Ram TG, Bissell MJ, Development. 105:223-235, 1989; Simian M, Bissell MJ, J Cell Biol...
2019: Methods in Molecular Biology
Khalid I W Kane, Edinson Lucumi Moreno, Siham Hachi, Moriz Walter, Javier Jarazo, Miguel A P Oliveira, Thomas Hankemeier, Paul Vulto, Jens C Schwamborn, Martin Thoma, Ronan M T Fleming
Parkinson's disease is a slowly progressive neurodegenerative disease characterised by dysfunction and death of selectively vulnerable midbrain dopaminergic neurons and the development of human in vitro cellular models of the disease is a major challenge in Parkinson's disease research. We constructed an automated cell culture platform optimised for long-term maintenance and monitoring of different cells in three dimensional microfluidic cell culture devices. The system can be flexibly adapted to various experimental protocols and features time-lapse imaging microscopy for quality control and electrophysiology monitoring to assess cellular activity...
February 11, 2019: Scientific Reports
Taylor Smith, Kevin Affram, Errol Bulumko, Edward Agyare
Purpose: The studies investigate the anticancer activity of 5-fluorouracil (5-FU)-hyaluronidase (Hase) enzyme-loaded chitosan nanoparticles (5-FUChnps) using three-dimensional (3D) spheroid HCT-116 culture. Hase-loaded nanoparticles (Chnps) have recently been used to improve the efficacy of chemotherapeutic drugs for cancer treatment. It has been found that administration of Hase improves tumor vessel densities and increase perfusion within tumor. Methods: Particle size and zeta potential of the nanoparticles were determined using a particle size analyzer while Fourier transform infrared (FTIR) was used to investigate the interactions among the various components making up the chitosan nanoparticles...
October 2018: Journal of Nature and Science
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