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Junnan Gu, Jennifer M Thomas-Ahner, Kenneth M Riedl, Michael T Bailey, Yael Vodovotz, Steven J Schwartz, Steven K Clinton
SCOPE: Black raspberries (BRB) are a rich source of bioactive phytochemicals, including anthocyanins and ellagitannins. These phytochemicals are poorly absorbed and we hypothesize that upon reaching the colon they may be transformed by gut microbiota into various metabolites that may impact the colonic mucosa or upon absorption have systemic bioactivity. The objective of this study was to define the impact of a BRB-containing diet on the colon microbiome in mice and quantify the phytochemical metabolites in the colon contents and the circulation...
February 14, 2019: Molecular Nutrition & Food Research
A Girardeau, C Puentes, S Keravec, P Peteuil, I C Trelea, F Fonseca
AIM: Investigate the effect of a broad spectrum of culture conditions on the acidification activity and viability of Carnobacterium maltaromaticum CNCM I-3298, the main technological properties that determine the shelf-life of biological Time-Temperature Integrator labels. METHODS AND RESULTS: Cells were cultivated at different temperatures (20 to 37 °C) and pH (6 to 9·5) according to a modified central composite design and harvested at increasing times up to 10 h of stationary phase...
February 14, 2019: Journal of Applied Microbiology
Tingzhang Hu, Yuanli Liu, Shanshan Zhu, Juan Qin, Wenping Li, Nong Zhou
The group 5 LEA (late embryogenesis abundant) proteins are an atypical LEA protein group, which is associated with resistance to multiple stresses. In this study, OsLea14-A gene was isolated from Oryza sativa L., which encodes a 5C LEA protein with 151 amino acids. The qPCR analysis showed that OsLea14-A expressed in all tissues and organs at all times. The expression of OsLea14-A in the panicles of plumping stage were dramatically increased. The heterologous expression of OsLea14-A in Escherichia coli improved its growth performance under salinity, desiccation, high temperature, and freeze-thaw stresses...
February 14, 2019: Environmental Science and Pollution Research International
Stine Gry Kristensen, Susanne Elisabeth Pors, Liv la Cour Poulsen, Signe Taasti Andersen, Yu Wakimoto, Claus Yding Andersen
INTRODUCTION: Young women with a cancer diagnosis often have very little time to decide whether or not to commence on fertility preserving strategies before initiating potentially sterilizing cancer treatment. Minimizing the interval from opting for fertility preservation to completion of the procedure will reduce the potential risk of delaying cancer treatment. In the current study, we have evaluated the period of time from referral to ovarian tissue cryopreservation (OTC) to actual freezing of the tissue in a cohort of Danish women...
February 13, 2019: Acta Obstetricia et Gynecologica Scandinavica
Alessandra Alteri, Valerio Pisaturo, Daniela Nogueira, Arianna D'Angelo
The aim of this review is to provide current knowledge on fertility preservation for nonmedical reasons in women willing to postpone childbearing. The topic is highly debatable starting from the disagreement about its terminology, the number of eggs necessary to predict chances of success, and the safety and socio/ethical point of view. Cost analysis and discrepancies among countries recommendations and regulations are described to confirm the controversies and unsolved issues around this very interesting topic...
February 13, 2019: Acta Obstetricia et Gynecologica Scandinavica
Qianxin Wang, Zhiyong Qian, Bo Liu, Jinglong Liu, Liang Zhang, Juan Xu
In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side. PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings. AgNPs were characterized using a transmission electron microscope. The morphologies of the composite dressing were examined under a scanning electron microscope. The silver content of the dressing was measured by inductively coupled plasma mass spectrometry...
February 12, 2019: Journal of Biomaterials Science. Polymer Edition
Marja Roitto, Sirkka Sutinen, Ai-Fang Wang, Timo Domisch, Tarja Lehto, Tapani Repo
Soil waterlogging is predicted to increase in the future climate in boreal regions due to increased precipitation. Snowmelt periods in winter may also become more common and further increase the amount of water in soil. It is not well known how waterlogging and soil freezing during winter affect the physiology, phenology and growth of trees. Our aim was to study the below- and aboveground responses of Scots pine (Pinus sylvestris L.) saplings to waterlogging (WL) in frozen (Fr) and unfrozen (NoFr) soils in a growth chamber experiment...
February 7, 2019: Tree Physiology
Jianzhao Li, Xinhui Yan, Qinsong Yang, Yunjing Ma, Bo Yang, Juan Tian, Yuanwen Teng, Songling Bai
PpCBF2 directly binds to the promoters of PpCBF3 and PpCBF4 to activate their expressions and selectively regulates PpDAMs during the leaf bud endodormancy process of 'Wonhwang' pear (Pyrus pyrifolia). Endodormancy is critical for temperate plant survival under freezing winter conditions, and low temperature is a vital environmental factor in endodormancy regulation. A C-repeat binding factor (CBF) has been found to regulate important DAM transcription factors during endodormancy in pear (Pyrus pyrifolia). In this study, we analyzed the regulation of pear DAM genes by CBFs in further detail...
February 12, 2019: Plant Molecular Biology
Mari-Anne Vals, Angel Ashikov, Pilvi Ilves, Dagmar Loorits, Qiang Zeng, Rita Barone, Karin Huijben, Jolanta Sykut-Cegielska, Luísa Diogo, Abdallah F Elias, Robert S Greenwood, Stephanie Grunewald, Peter M van Hasselt, Jiddeke M van de Kamp, Grazia Mancini, Agnieszka Okninska, Sander Pajusalu, Pauline M Rudd, Cecilie F Rustad, Ramona Salvarinova, Bert B A de Vries, Nicole I Wolf, Bobby G Ng, Hudson H Freeze, Dirk J Lefeber, Katrin Õunap
SLC35A2-CDG is caused by mutations in the X-linked SLC35A2 gene encoding the UDP-galactose transporter. SLC35A2 mutations lead to hypogalactosylation of N-glycans. SLC35A2-CDG is characterized by severe neurological symptoms and, in many patients, early-onset epileptic encephalopathy. In view of the diagnostic challenges, we studied the clinical, neuroradiological, and biochemical features of 15 patients (11 females and 4 males) with SLC35A2-CDG from various centers. We describe nine novel pathogenic variations in SLC35A2...
January 12, 2019: Journal of Inherited Metabolic Disease
E Maris, A Ferrieres-Hoa, A Gala, A Coffy, E Vintejoux, N Ranisavljevic, S Hamamah
OBJECTIVE: It is already known that children born after slow frozen embryo replacement have a significantly higher birth weight compared to children born after fresh embryo transfer. Similar data have been reported related to frozen embryo transfer using an open vitrification system. However, few data relative to birth weight using a complete embryo closed vitrification system has been reported. The purpose of this study was to know if frozen embryo in closed vitrification system is associated with a higher birth weight compared to fresh embryo replacement...
February 8, 2019: Gynecologie, Obstetrique, Fertilite & Senologie
Kemal Ozgur, Hasan Bulut, Murat Berkkanoglu, Peter Humaidan, Kevin Coetzee
The present study was performed to investigate whether maternal body mass index (BMI) affected the live birth (LB) outcomes of frozen embryo transfers (FET) in patients who underwent freeze-all treatment cycles. The autologous intracytoplasmic sperm injection (ICSI) cycles with blastocyst freeze-all cycles performed between February 2015 and January 2016 were retrospectively investigated. The 1188 subsequent FET performed were grouped according to maternal BMI classes for analysis; underweight (<18.5 kg/m2 ; 3...
February 11, 2019: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
Paloma Serrano, Mashal Alawi, Jean-Pierre de Vera, Dirk Wagner
Numerous preflight investigations were necessary prior to the exposure experiment BIOMEX on the International Space Station to test the basic potential of selected microorganisms to resist or even to be active under Mars-like conditions. In this study, methanogenic archaea, which are anaerobic chemolithotrophic microorganisms whose lifestyle would allow metabolism under the conditions on early and recent Mars, were analyzed. Some strains from Siberian permafrost environments have shown a particular resistance...
February 2019: Astrobiology
Alison M Bacon, Chloe McDaid, Natasha Williams, Philip J Corr
BACKGROUND: Academic dishonesty (AD) is an increasing challenge for universities worldwide. The rise of the Internet has further increased opportunities for students to cheat. AIMS: In this study, we investigate the role of personality traits defined within Reinforcement Sensitivity Theory (RST) as potential determinants of AD. RST defines behaviour as resulting from approach (Reward Interest/reactivity, goal-drive, and Impulsivity) and avoidance (behavioural inhibition and Fight-Flight-Freeze) motivations...
February 11, 2019: British Journal of Educational Psychology
Daniel Clarke, Adi Idris, Nigel A J McMillan
Liposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage at room temperature (RT), 4°C or -20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures...
2019: PloS One
Thiago Guimarães Osório, Saverio Iacopino, Hugo-Enrique Coutiño, Erwin Ströker, Juan Sieira, Francesca Salghetti, Varnavas Varnavas, Muryo Terasawa, Gaetano Paparella, Lucio Capulzini, Riccardo Maj, Yves De Greef, Pedro Brugada, Carlo de Asmundis, Gian-Battista Chierchia
PURPOSE: The purpose of this study was to clarify the behavior of the luminal esophageal temperature (LET) in a cohort of patients undergoing second-generation cryoballoon (CB-A) for pulmonary vein isolation (PVI) and additional left atrium posterior wall (LAPW) ablation by means of CB-A. METHODS: Thirty patients with symptomatic persistent AF (PersAF), having undergone PVI + LAPW cryoballoon ablation with LET monitoring. RESULTS: Interruption of the application due to a LET below 15 °C occurred in 5 patients (16...
February 7, 2019: Journal of Interventional Cardiac Electrophysiology: An International Journal of Arrhythmias and Pacing
Susan E Anthony, Brent J Sinclair
Although many arthropods are freeze tolerant (able to withstand internal ice), small-bodied terrestrial arthropods such as mites are thought to be constrained to freeze avoidance. We field-collected active adult red velvet mites, Allothrombium sp. (Trombidiidae), in winter in Southwestern Ontario, Canada, where temperatures drop below -20°C. These mites froze between -3.6° and -9.2°C and survived internal ice formation. All late-winter mites survived being frozen for 24 h at -9°C, and 50% survived 1 wk...
March 2019: Physiological and Biochemical Zoology: PBZ
Nicolaas I Bohnen, Prabesh Kanel, Zhi Zhou, Robert A Koeppe, Kirk A Frey, William T Dauer, Roger L Albin, Martijn L T M Müller
OBJECTIVE: Postural instability and gait difficulties (PIGD) represent debilitating disturbances in Parkinson's disease (PD). Prior acetylcholinesterase PET imaging studies implicate cholinergic changes as significant contributors to PIGD features. These studies were limited in quantification of striatal cholinergic synapse integrity. Vesicular acetylcholine transporter (VAChT) PET ligands are better suited for evaluation of high binding areas. We examined associations between regional VAChT expression and freezing of gait (FoG) and falls...
February 5, 2019: Annals of Neurology
Sumate Tantratian, Apinya Sae-Ngow, Chidphong Pradistsuwan, Chuenjit Prakitchaiwattana, Charida Pukahuta
Kluyveromyces marxianus is an aerobic yeast and is interested to be applied in many industries. This research was aimed to study the effect of the sterol alternative to ergosterol on the freezing stress of K. marxianus UBU1-11, a thermotolerant yeast. The 0-9 mgL-1 stigmasterol were added to the YM broth and applied for culturing. The growth of all conditions were not interfered by the addition of stigmasterol. The intra-cellular sterol content was detected in the medium with 5 mgL-1 stigmasterol and higher, where the maximum content was 0...
February 1, 2019: Journal of Bioscience and Bioengineering
Emma Browne, Frank Peeters, Melanie Priston, P T Marquis
INTRODUCTION: The British Antarctic Survey Medical Unit works in a very remote area of the world, with several Antarctic bases receiving only a single annual resupply of consumable goods. Pharmaceuticals supplied in this manner will often be approaching or past the end of their nominal shelf life before the following year's resupply. Drugs are transported from the UK via ship; the hold is not temperature controlled, and the ship crosses through the tropics (air temperature 25-30°C for approximately 3 wk)...
February 1, 2019: Wilderness & Environmental Medicine
Xin-Yu Wang, Zhen Jin, Xiao Ling, Chang-Shun Liu, Xiao-Mei Tan
A specific and selective UPLC-MS/MS method was developed and validated for the simultaneous determination of isoflavonoids(3'-hydroxy puerarin, puerarin, daidzin, daidzein, genistin, genistein), flavonoids (baicalin, baicalein, wogonoside, wogonin, liquiritin)and alkaloids(berberine, jatrorrhizine, palmatine)(14 bioactive compounds) of Gegen Qinlian Decoction(GQD) in plasma. The pharmacokinetics characteristics of 14 bioactive compounds were study after oral administration of GQD at a single dose to rats. Prednisolone was used as the internal standard of liquiritin, and naringin was used as the internal standard of the other thirteen analytes...
December 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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