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Adam M Sandor, Robin S Lindsay, Nathan Dyjack, Jennifer C Whitesell, Cydney Rios, Brenda J Bradley, Kathryn Haskins, David V Serreze, Aron M Geurts, Yi-Guang Chen, Max A Seibold, Jordan Jacobelli, Rachel S Friedman
Type 1 diabetes (T1D) is a T cell mediated autoimmune disease that affects more than 19 million people with incidence increasing rapidly worldwide. For T cells to effectively drive T1D, they must first traffic to the islets and extravasate through the islet vasculature. Understanding the cues that lead to T cell entry into inflamed islets is important because diagnosed T1D patients already have established immune infiltration of their islets. Here we show that CD11c+ cells are a key mediator of T cell trafficking to infiltrated islets in non-obese diabetic (NOD) mice...
2019: Frontiers in Immunology
Alina Uifalean, Hermann Rath, Elke Hammer, Corina Ionescu, Cristina Adela Iuga, Michael Lalk
PURPOSE: The aim of this study was to evaluate the anti-angiogenic properties of soy isoflavones using two breast cancer cell lines, by measuring the concentration of 30 cytokines involved in angiogenesis using a multiplex glass slide ELISA-based array. METHODS: Estrogen-dependent MCF-7 cells and estrogen-independent MDA-MB-231 cells were exposed to genistein (Gen), daidzein (Dai) and a soy seed extract (Ext) for 72 hrs, at selected concentration levels. The conditioned medium was analyzed using a glass slide, multiplex sandwich ELISA-based platform with fluorescent detection which allowed the identification and the quantification of 30 angiogenesis-related cytokines...
December 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Qi Xiao, Xiaoyan Zhu, Shaonan Yang, Jing Wang, Ruihua Yin, Jinyang Song, Aijun Ma, Xudong Pan
Endothelial inflammation characterizes the early stages of atherosclerosis. CXCL16 is a protein that functions as both a chemokine and adhesion molecule, playing a crucial role in the pathogenesis of atherosclerosis. However, it is uncertain if LPS, a major inducer of inflammation, affects CXCL16 expression in endothelial cells and whether miR-146a, a negative regulator of atherosclerosis, participates in this process. The present study showed that exposure of human umbilical vein endothelial cells (HUVECs) to LPS induced the overexpression of CXCL16, TLR4 and NF-κB, and this induction was blocked by the TLR4 inhibitor TAK-242...
January 30, 2019: International Immunopharmacology
Jana C Mossanen, Marlene Kohlhepp, Alexander Wehr, Oliver Krenkel, Anke Liepelt, Anjali A Roeth, Diana Möckel, Felix Heymann, Twan Lammers, Nikolaus Gassler, Juliane Hermann, Joachim Jankowski, Ulf P Neumann, Tom Luedde, Christian Trautwein, Frank Tacke
BACKGROUND & AIMS: Inflammation in the liver provokes fibrosis but inflammation is also important for tumor surveillance. Inhibitors of chemokine pathways, such as CXCL16 and CXCR6 regulation of lymphocyte trafficking, are being tested as anti-fibrotic agents, but their effects on development of hepatocellular carcinoma (HCC) are unclear. We assessed the roles of CXCR6-dependent immune mechanisms in hepatocarcinogenesis. METHODS: C57BL/6J wild-type (WT) mice and CXCR6-deficient mice (Cxcr6eGfp/eGfp ) were given injections of diethylnitrosamine (DEN) to induce liver cancer, and α-galactosylceramide to activate natural killer T (NKT) cells...
January 30, 2019: Gastroenterology
Ragni H Mørch, Ingrid Dieset, Ann Færden, Elina J Reponen, Sigrun Hope, Eva Z Hoseth, Erlend S Gardsjord, Monica Aas, Trude Iversen, Inge Joa, Gunnar Morken, Ingrid Agartz, Ingrid Melle, Pål Aukrust, Srdjan Djurovic, Thor Ueland, Ole A Andreassen
BACKGROUND: Inflammation and immune activation have been implicated in the pathogenesis of severe mental disorders and cardiovascular disease (CVD). Despite high level of comorbidity, many studies of the immune system in severe mental disorders have not systematically taken cardiometabolic risk factors into account. METHODS: We investigated if inflammatory markers were increased in schizophrenia (SCZ) and affective (AFF) disorders independently of comorbid CVD risk factors...
January 28, 2019: Psychological Medicine
Xiao-Qiu Wang, Wen-Jie Zhou, Xin-Xin Hou, Qiang Fu, Da-Jin Li
In this article, published online 27 March 2018, in the "ACKNOWLEDGEMENTS", it should be supplemented as follows: "This study was funded by a grant from the National Natural Science Foundation of China, number 81490744, awarded to Da-Jin Li." The authors regret the omission.
January 22, 2019: Cellular & Molecular Immunology
M A El-Gamasy, W El-Naghy
One of the challenges of treating patients with lupus nephritis (LN) is to assess disease activity. The aim of this study was to measure the urinary neutrophil gelatinase-associated lipocalin (uNGAL) and urinary soluble chemokine (C-X-C motif) ligand 16 (CXCL16) levels in children and adolescents with systemic lupus erythematosus (SLE) and investigate whether they are elevated in active LN. This study was conducted on 80 patients diagnosed as SLE by the Systemic Lupus International Collaborating Clinics criteria and 60 apparently healthy individuals as controls...
November 2018: Indian Journal of Nephrology
Junqiong Huang, Huan Yue, Tao Jiang, Jing Gao, Yu Shi, Bin Shi, Xiaoxue Wu, Xiaoqin Gou
Interleukin 31 (IL-31) is a four-helix cytokine made predominantly by Th2 CD4+ T cells. It was initially identified as being associated with the promotion of atopic dermatitis, where increased levels of IL-31 levels have been found and IL-31 induced the expression of proinflammatory cytokines and chemokines in a human bronchial epithelial cell line. However, subsequent study has shown that IL-31RA knockout mice developed exacerbated type 2 inflammation in the lung following infection with Schistosoma mansoni eggs...
January 15, 2019: Biology Open
Jie Mei, Yuan Yan, Shi-Yuan Li, Wen-Jie Zhou, Qun Zhang, Ming-Qing Li, Hai-Xiang Sun
Decidualization renders the endometrium transiently receptive to an implanting blastocyst although the underlying mechanisms remain incompletely understood. The aim of this study was to determine the role of chemokine CXCL16 and its receptor CXCR6 in the decidualization during pregnancy. Here, the expression of CXCL16 was investigated in endometrial tissues, decidua, and placenta in this study. Compared with endometrial tissue, protein expression of CXCL16 was significantly higher in tissues from the fertile control samples, especially in villus...
January 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
Jinou Wang, Chang Liu, Xiaoying Chang, Yafei Qi, Zhi Zhu, Xianghong Yang
Background: Peritoneal metastasis frequently occurs in patients with advanced ovarian cancer and is the main basis for a poor prognosis. The mechanism underlying preferential ovarian cancer spread to the peritoneum is not well understood. Methods: Herein, we investigated the significance and mechanism underlying fibrosis of mesothelial cells promoting peritoneal implantation of ovarian cancer. We have assessed the mesothelial cell fibroblast transformation process in peritoneal tissues of omentum and fibrotic mesothelial cell release of chemokines to promote dissemination by scanning electron microscopy, ELISA, Western blot, and Transwell chamber assay...
2018: Cancer Management and Research
Anouk A J Hamers, Huy Q Dinh, Graham D Thomas, Paola Marcovecchio, Amy Blatchley, Catherine S Nakao, Cheryl Kim, Chantel McSkimming, Angela M Taylor, Anh T Nguyen, Coleen A McNamara, Catherine C Hedrick
Objective- Three distinct human monocyte subsets have been identified based on the surface marker expression of CD14 and CD16. We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high dimensionality of mass cytometry together with the FlowSOM clustering algorithm to accurately identify and define monocyte subsets in blood of healthy human subjects and those with coronary artery disease (CAD). To study the behavior and functionality of the newly defined monocyte subsets, we performed RNA sequencing, transwell migration, and efferocytosis assays...
November 15, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
Francesca Lepore, Giuseppina D'Alessandro, Fabrizio Antonangeli, Antonio Santoro, Vincenzo Esposito, Cristina Limatola, Flavia Trettel
Microglia are patrolling cells that sense changes in the brain microenvironment and respond acquiring distinct phenotypes that can be either beneficial or detrimental for brain homeostasis. Anti-inflammatory microglia release soluble factors that might promote brain repair; however, in glioma, anti-inflammatory microglia dampen immune response and promote a brain microenvironment that foster tumor growth and invasion. The chemokine CXCL16 is expressed in the brain, where it is neuroprotective against brain ischemia, and it has been found to be over-expressed in glioblastoma (GBM)...
2018: Frontiers in Immunology
Jaemin Lee, Tae Heung Kang, Wonbeak Yoo, Hyunji Choi, Seongyea Jo, Kyungsu Kong, Sang-Rae Lee, Sun-Uk Kim, Ji-Su Kim, Duck Cho, Janghwan Kim, Jeong-Yoon Kim, Eun-Soo Kwon, Seokho Kim
Natural killer (NK) cells are primary immune cells that target cancer cells and can be used as a therapeutic agent against pancreatic cancer (PC). Despite their usefulness, NK-cell therapy is limited by tumor cell inhibition of NK-cell homing to tumor sites, thereby preventing a sustained antitumor immune response. One approach to successful cancer immunotherapy is to increase trafficking of NK cells to tumor tissues. Here, we developed an antibody-based NK cell-homing protein, named NK cell-recruiting protein-conjugated antibody (NRP-body)...
December 4, 2018: Cancer Immunology Research
Yan Li, Shihao Chen, Yafei Chi, Yiran Yang, Xiwen Chen, Huating Wang, Zhe Lv, Jingjing Wang, Linjie Yuan, Ping Huang, Kewu Huang, Chris J Corrigan, Wei Wang, Sun Ying
ILC2s are implicated in asthma pathogenesis, but little is known about the mechanisms underlying their accumulation in airways. We investigated the time course of ILC2 accumulation in different tissues in murine models of asthma induced by a serial per-nasal challenge with ovalbumin (OVA), house dust mice (HDM), IL-25 and IL-33 and explored the potential roles of ILC2-attracting chemokines in this phenomenon. Flow cytometry was used to enumerate ILC2s at various time points. The effects of cytokines and chemokines on ILC2 migration were measured in vitro using a chemotaxis assay and in vivo using small animal imaging...
November 22, 2018: Cellular & Molecular Immunology
Christa Trattnig, Muammer Üçal, Carmen Tam-Amersdorfer, Angela Bucko, Ulrike Zefferer, Gerda Grünbacher, Markus Absenger-Novak, Kristin Anna Öhlinger, Klaus Kraitsy, Daniel Hamberger, Ute Schaefer, Silke Patz
MiR-451a is best known for its role in erythropoiesis and for its tumour suppressor features. Here we show a role for miR-451a in neuronal differentiation through analysis of endogenous and ectopically expressed or silenced miR-451a in Ntera2/D1 cells during neuronal differentiation. Furthermore, we compared neuronal differentiation in the dentate gyrus of hippocampus of miR-451a-/- and wild type mice. MiR-451a overexpression in lentiviral transduced Ntera2/D1 cells was associated with a significant shifting of mRNA expression of the developmental markers Nestin, βIII Tubulin, NF200, DCX and MAP2 to earlier developmental time points, compared to control vector transduced cells...
2018: PloS One
Takashi Kato, Man Hagiyama, Akihiko Ito
A disintegrin and metalloproteinases (ADAMs) are a Zn2+ -dependent transmembrane and secreted metalloprotease superfamily, so-called "molecular scissors," and they consist of an N-terminal signal sequence, a prodomain, zinc-binding metalloprotease domain, disintegrin domain, cysteine-rich domain, transmembrane domain and cytoplasmic tail. ADAMs perform proteolytic processing of the ectodomains of diverse transmembrane molecules into bioactive mediators. This review summarizes on their most well-known members, ADAM10 and 17, focusing on the kidneys...
2018: Frontiers in Cell and Developmental Biology
Gunnthorunn Sigurdardottir, Anna-Karin Ekman, Deepti Verma, Charlotta Enerbäck
BACKGROUND: In psoriasis, a common immune-mediated disease affecting 2-3% of the population worldwide, there is an increased prevalence of extracutaneous diseases including obesity, the metabolic syndrome, and cardiovascular disease. This is believed to be linked to systemic inflammation. In previous studies, we have explored various markers in plasma and serum to characterize the ongoing systemic inflammation in psoriasis patients compared to controls. We have identified several markers that were altered in psoriasis patients, but which all were unresponsive to narrowband UVB (NB-UVB) treatment...
2018: Dermatology: International Journal for Clinical and Investigative Dermatology
Kun Ling Ma, Tie Kai Gong, Ze Bo Hu, Yang Zhang, Gui Hua Wang, Liang Liu, Pei Pei Chen, Jian Lu, Chen Chen Lu, Bi Cheng Liu
BACKGROUND: Increased plasma level of lipoprotein(a) (Lpa) is a risk factor of cardiovascular diseases. This study aimed to explore the role of Lpa in the progression of atherosclerosis in patients with end-stage renal disease (ESRD) and to investigate whether its potential mechanism is mediated by CXC chemokine ligand 16 (CXCL16) and low-density lipoprotein receptor (LDLr). METHODS: This is a retrospective clinical study. From January 2015 to April 2016, forty-six ESRD patients from Danyang First People's Hospital were investigated...
August 2, 2018: BMC Nephrology
Lina Jiang, Mei Yang, Xi Li, Yijin Wang, Guangde Zhou, Jingmin Zhao
Context: Nonalcoholic fatty liver disease (NAFLD) is a focus of attention because of its prevalence. CXC motif ligand 16 (CXCL16) has been studied in inflammatory and metabolic diseases. Objective: To investigate the role of CXCL16 in steatosis and fibrosis in patients with NAFLD. Design: Liver specimens and sera of patients with NAFLD were collected from 2012 to 2017. Setting: Beijing 302 Hospital. Participants: 117 patients with NAFLD and 15 healthy controls...
November 1, 2018: Journal of Clinical Endocrinology and Metabolism
Lan Hong, Shengtan Wang, Wei Li, Dongcai Wu, Wangsheng Chen
This study investigated the underlying mechanism by which C-X-C motif chemokine ligand 16 (CXCL16)/C-X-C motif chemokine receptor 6 (CXCR6) signaling is activated by tumor-associated macrophages and assists in regulating the metastasis of ovarian carcinoma. Specimens of ovarian carcinoma tissue and adjacent tissue were collected from 20 ovarian carcinoma patients. Human THP-1 cells were induced to differentiate into macrophages, which were then co-cultured with SKOV3 cells and low concentrations of tumor necrosis factor-α (TNF-α) to simulate the inflammatory microenvironment of ovarian carcinoma...
September 2018: Pathology, Research and Practice
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