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https://read.qxmd.com/read/30785503/bone-callus-formation-is-highly-disrupted-by-dietary-restriction-in-growing-rats-sustaining-a-femoral-fracture1
#1
Iara Inácio Botega, Ariane Zamarioli, Patrícia Madalena San Gregório Guedes, Raquel Assed Bezerra da Silva, João Paulo Mardegan Issa, Mariana Maloste Butezloff, Yara Terezinha Corrêa Silva Sousa, João Paulo Bianchi Ximenez, José Batista Volpon
PURPOSE: To evaluate the effects of food restriction on fracture healing in growing rats. METHODS: Sixty-eight male Wistar rats were assigned to two groups: (1) Control and (2) Dietary restriction. After weaning the dietary restricted animals were fed ad libitum for 42 days with 50% of the standard chow ingested by the control group. Subsequently, the animals underwent bone fracture at the diaphysis of the right femur, followed by surgical stabilization of bone fragments...
February 14, 2019: Acta Cirúrgica Brasileira
https://read.qxmd.com/read/30785308/mthpc-loaded-extracellular-vesicles-outperform-liposomal-and-free-mthpc-formulations-by-an-increased-stability-drug-delivery-efficiency-and-cytotoxic-effect-in-tridimensional-model-of-tumors
#2
Marie Millard, Ilya Yakavets, Max Piffoux, Amanda Brun, Florence Gazeau, Jean-Michel Guigner, Jordane Jasniewski, Henri-Pierre Lassalle, Claire Wilhelm, Lina Bezdetnaya
Efficient photodynamic therapy with meta-tetra(hydroxyphenyl)chlorine requires the application of specific nanoformulations. mTHPC liposomal formulation (Foslip® ) demonstrated favorable pharmacokinetics properties. However, rapid liposomes destruction in circulation and rapid mTHPC release impedes Foslip® applications. Alternatively, mTHPC nanovectorization using extracellular vesicles (EVs) could be an attractive option. EVs are naturally secreted by the organism to play a role in intercellular communication due to the capacity to transport proteins and nucleic acids...
November 2018: Drug Delivery
https://read.qxmd.com/read/30718645/optimization-of-pulmonary-vasculature-tridimensional-phenotyping-in-the-rat-fetus
#3
Emrah Aydin, Brittany Levy, Marc Oria, Hussam Nachabe, Foong-Yen Lim, Jose L Peiro
Comparative, functional, developmental, and some morphological studies on animal anatomy require accurate visualization of three-dimensional structures. Nowadays, several widely applicable methods exist for non-destructive whole-mount imaging of animal tissues. The purpose of this study was to optimize specimen preparation and develop a method for quantitative analysis of the total pulmonary vasculature in fetal rats. Tissues were harvested at E21 and fetuses fixed overnight in 4% paraformaldehyde/phosphate buffered saline...
February 4, 2019: Scientific Reports
https://read.qxmd.com/read/30710397/stabilizing-oxygen-lattice-and-reversible-oxygen-redox-chemistry-through-structural-dimensionality-in-li-rich-cathode-oxides
#4
Enyue Zhao, Qinghao Li, Fanqi Meng, Jue Liu, Junyang Wang, Lunhua He, Zheng Jiang, Qinghua Zhang, Xiqian Yu, Lin Gu, Wanli Yang, Hong Li, Fangwei Wang, Xuejie Huang
Lattice oxygen redox (l-OR) has become an essential complementation for the traditional transition-metal (TM) redox charge compensation to achieve the high capacity in Li-rich cathode oxides. However, the understanding of l-OR chemistry remains elusive, and a critical question is the structural effect on the stability of l-OR reactions. Herein, the coupling between l-OR and structure dimensionality is researched. We reveal that the oxygen lattice structure evolution upon the l-OR in Li-rich TM oxides with tridimensional (3D)-disordered cation framework is relatively stable, directly contrasting the obviously distorted oxygen lattice framework in Li-rich oxides with two-dimensional (2D)/3D-ordered cation structure...
February 1, 2019: Angewandte Chemie
https://read.qxmd.com/read/30689659/low-field-magnetic-resonance-imaging-of-the-equine-distal-interphalangeal-joint-comparison-between-weight-bearing-and-non-weight-bearing-conditions
#5
Laurence Evrard, Fabrice Audigié, Lélia Bertoni, Sandrine Jacquet, Jean-Marie Denoix, Valeria Busoni
This descriptive study aimed to compare the magnetic resonance appearance of the distal interphalangeal joint articular cartilage between standing weight-bearing and non-weight-bearing conditions. Ten forefeet of live horses were scanned in a standing low-field magnetic resonance system (0.27 T). After euthanasia for reasons unrelated to the study, the non-weight-bearing isolated feet were scanned in a vertical positioning reproducing limb orientation in live horses. The same acquisition settings as during the weight-bearing examination were used...
2019: PloS One
https://read.qxmd.com/read/30689295/spatial-organization-of-the-gene-expression-hardware-in-pseudomonas-putida
#6
Juhyun Kim, Angel Goñi-Moreno, Belén Calles, Víctor de Lorenzo
The localization of ribosomes, RNA polymerase (RNAP) and the nucleoid of Pseudomonas putida cells has been inspected with genetic, microscopical and physiological approaches. To this end, strains of P. putida were constructed with fluorescent tags to either ribosomal proteins or the RNAP or both. Their relative positions in respect to the bacterial DNA revealed the separation of the ribosomal pool from the nucleoid, which however co-localized entirely with the tridimensional distribution of RNAP. This split was kept under all growth conditions: exponential vs...
January 28, 2019: Environmental Microbiology
https://read.qxmd.com/read/30684292/exome-sequencing-identifies-a-de-novo-foxa2-variant-in-a-patient-with-syndromic-diabetes
#7
Caroline Stekelenburg, Karine Gerster, Jean-Louis Blouin, Mariarosaria Lang-Muritano, Michel Guipponi, Federico Santoni, Valerie M Schwitzgebel
OBJECTIVE: When diabetes is associated with congenital malformations, without autoimmune antibodies, a genetic cause is suspected. Here, we aimed to identify a defective gene that led to diabetes. RESEARCH DESIGN AND METHODS: We performed an exome analysis of an index case and his healthy parents. RESULTS: The child presented with childhood-onset diabetes, congenital hypopituitarism, cardiac malformation, and anal atresia. A DNA analysis revealed a heterozygous de novo pathogenic variant in the developmental transcription factor, forkhead box A2 (FOXA2)...
January 26, 2019: Pediatric Diabetes
https://read.qxmd.com/read/30681075/characterization-of-a-novel-decellularized-bone-marrow-scaffold-as-an-inductive-environment-for-hematopoietic-stem-cells
#8
Juares E Romero Bianco, Renata Giardini Rosa, Ada Congrains-Castillo, Paulo P Joazeiro, Stephen D Waldman, Joanna F Weber, Sara Teresinha Olalla Saad
Due to the increasing demand for a bone marrow study model, we developed a natural scaffold from decellularized bovine bone marrow (DeBM). The obtained bioscaffold was analyzed after the decellularization process; histological staining, scanning and transmission electron microscopy confirmed the preservation of its native 3D-architecture; including blood vessels and cell niches as well as the integrity of important components of the extracellular matrix; Collagen III, IV and fibronectin. In addition to biochemical composition, physical properties of the bone marrow were also conserved...
January 25, 2019: Biomaterials Science
https://read.qxmd.com/read/30680808/engineering-nio-nife-ldh-intersection-to-bypass-scaling-relationship-for-oxygen-evolution-reaction-via-dynamic-tridimensional-adsorption-of-intermediates
#9
Zhi-Wen Gao, Jie-Yu Liu, Xue-Min Chen, Xue-Li Zheng, Jing Mao, Hui Liu, Tian Ma, Lan Li, Wei-Chao Wang, Xi-Wen Du
Oxygen evolution reaction (OER) is a pivotal reaction in many technologies for renewable energy, such as water splitting, metal-air batteries, and regenerative fuel cells. However, this reaction is known to be kinetically sluggish and proceeds at rather high overpotential due to the universal scaling relationship, namely, the adsorption energies of intermediates are linearly correlated and cannot be optimized simultaneously. Several approaches have been proposed to break the scaling relationship by introducing additional active sites; however, positive experimental results are still absent...
January 25, 2019: Advanced Materials
https://read.qxmd.com/read/30660239/corrigendum-to-tridimensional-analysis-of-rotatory-subluxation-and-sagittal-spinopelvic-alignment-in-the-setting-of-adult-spinal-deformity-spine-deformity-5-4-2017-255-264
#10
Emmanuelle Ferrero, Renaud Lafage, Bassel G Diebo, Vincent Challier, Brice Illharreborde, Frank Schwab, Wafa Skalli, Pierre Guigui, Virginie Lafage
No abstract text is available yet for this article.
March 2019: Spine Deformity
https://read.qxmd.com/read/30660003/tensile-behavior-and-structural-characterization-of-pig-dermis
#11
Andrei Pissarenko, Wen Yang, Haocheng Quan, Katherine A Brown, Alun Williams, William G Proud, Marc A Meyers
Skin, the outermost layer of the body, fulfills a broad range of functions, protecting internal organs from damage and infection, while regulating the body's temperature and water content via the exchange of heat and fluids. It must be able to withstand and recover from extensive deformation and damage that can occur during growth, movement, and potential injuries. A detailed investigation of the evolution of the collagen architecture of the dermis as a function of deformation is conducted, which reveals new aspects that help us to understand the mechanical response of skin...
January 16, 2019: Acta Biomaterialia
https://read.qxmd.com/read/30654316/modelling-and-molecular-docking-studies-of-the-cytoplasmic-domain-of-wsc-family-full-length-ras2p-and-therapeutic-antifungal-compounds
#12
Vladimir Vélez-Segarra, Kelvin Carrasquillo-Carrión, Jorge J Santini-González, Yabdiel A Ramos-Valerio, Luis E Vázquez-Quiñones, Abiel Roche-Lima, José R Rodríguez-Medina, Elsie I Parés-Matos
Saccharomyces cerevisiae, the budding yeast, must remodel initial cell shape and cell wall integrity during vegetative growth and pheromone-induced morphogenesis. The cell wall remodeling is monitored and regulated by the cell wall integrity (CWI) signaling pathway. Wsc1p, together with Wsc2p and Wsc3p, belongs to a family of highly O-glycosylated cell surface proteins that function as stress sensors of the cell wall in S. cerevisiae. These cell surface proteins have the main role of activating the CWI signaling pathway by stimulating the small G-protein Rho1p, which subsequently activates protein kinase C (Pkc1p) and a mitogen activated protein (MAP) kinase cascade that activates downstream transcription factors of stress-response genes...
January 3, 2019: Computational Biology and Chemistry
https://read.qxmd.com/read/30652170/organogels-from-trehalose-difatty-ester-amphiphiles
#13
G Hibert, M Fauquignon, J-F Le Meins, D Pintori, E Grau, S Lecommandoux, H Cramail
Saccharide diesters have been recently shown to be excellent gelators of vegetable oils. In this paper, different fatty acid trehalose diesters were synthesized by a selective enzymatic transesterification performed only on the primary hydroxyl group of the trehalose. The resulting trehalose diesters demonstrated their ability to self-assemble in a large variety of edible vegetable oils with a minimum gelation concentration of 0.25 wt%/v. Microscopic analysis and X-ray scattering studies indicate that the gels are obtained by the self-assembly of trehalose diesters in crystalline fibers constituting the tridimensional network...
January 17, 2019: Soft Matter
https://read.qxmd.com/read/30645258/ronchi-and-moir%C3%A3-patterns-for-testing-spherical-and-aspherical-surfaces-using-deflectometry
#14
J A Arriaga-Hernández, A Jaramillo-Núñez
The aim of this paper is to compare the Ronchi test and the Moiré deflectometry applied to a new pattern. To do so, we used two Ronchi patterns (typical Ronchigrams) produced by two identical Ronchi rulings angularly displaced and placed close to the curvature radius of a mirror. The result obtained by superposing both Ronchigrams is our new pattern, a Moiré pattern of the Ronchigram (Moiré-Ronchigram). The Zernike aberration polynomial coefficients of the tridimensional wavefront for both mirrors are obtained as a result...
December 1, 2018: Applied Optics
https://read.qxmd.com/read/30642733/pretreatment-tumor-volume-and-tumor-sphericity-as-prognostic-factors-in-patients-with-oral-cavity-squamous-cell-carcinoma
#15
Achille Tarsitano, Francesco Ricotta, Laura Cercenelli, Barbara Bortolani, Salvatore Battaglia, Elisabetta Lucchi, Claudio Marchetti, Emanuela Marcelli
PURPOSE: This study was designed as a retrospective observational study, focusing on the correlation between the preoperative CT-scan tumor volume, tumor sphericity, and the disease-related prognosis. METHODS: A total of 30 consecutive patients, affected by primary oral cancer, were retrospectively identified from our oral cancer database. The preoperative images (DICOM data) for the study population were uploaded into a modular software package designed to convert patients' medical images into 3D digital models...
January 3, 2019: Journal of Cranio-maxillo-facial Surgery
https://read.qxmd.com/read/30632462/a-virtual-reality-environment-to-visualize-three-dimensional-patient-specific-models-by-a-mobile-head-mounted-display
#16
Maurizio Vertemati, Simone Cassin, Francesco Rizzetto, Angelo Vanzulli, Marco Elli, Gianluca Sampogna, Maurizio Gallieni
INTRODUCTION: With the availability of low-cost head-mounted displays (HMDs), virtual reality environments (VREs) are increasingly being used in medicine for teaching and clinical purposes. Our aim was to develop an interactive, user-friendly VRE for tridimensional visualization of patient-specific organs, establishing a workflow to transfer 3-dimensional (3D) models from imaging datasets to our immersive VRE. MATERIALS AND METHODS: This original VRE model was built using open-source software and a mobile HMD, Samsung Gear VR...
January 11, 2019: Surgical Innovation
https://read.qxmd.com/read/30620892/understanding-anatomy-of-the-petrous-pyramid-a-new-compartmental-approach
#17
Mamdouh Tawfik-Helika, Patrick Mertens, Guilherme Ribas, Martin Catala, Ramez Kirollos, Timothée Jacquesson
BACKGROUND: Surgical anatomy of the petrous pyramid has always been a challenge, especially in the beginning of the training process. Providing an easier, holistic approach can be of help to everyone with interest in learning and teaching skull base anatomy. We present the complex organization of the petrous pyramid anatomy using a new compartmental approach that is simple to understand and remember. METHODS: The contents of the petrous pyramid of eight temporal bones were exposed through progressive drilling of the superior surface...
January 5, 2019: World Neurosurgery
https://read.qxmd.com/read/30607867/preoperative-assessment-of-simple-and-complex-anorectal-fistulas-tridimensional-endoanal-ultrasound-magnetic-resonance-both
#18
Antonio Brillantino, Francesca Iacobellis, Alfonso Reginelli, Luigi Monaco, Biagio Sodano, Giuseppe Tufano, Antonio Tufano, Mauro Maglio, Maurizio De Palma, Natale Di Martino, Adolfo Renzi, Roberto Grassi
PURPOSE: The purpose of the study is to evaluate the diagnostic value of tridimensional endoanal ultrasound (3D-EAUS) and magnetic resonance (MR) in the preoperative assessment of both simple and complex anorectal fistulas. METHODS: All the patients referred for the treatment of anal fistulas were enrolled in this study and underwent, as preoperative assessment, anamnestic evaluation, clinical examination, and unenhanced and H2O2-enhanced 3D-EAUS and MR. The results of imaging evaluation were compared with surgical findings, considered as reference standard...
January 3, 2019: La Radiologia Medica
https://read.qxmd.com/read/30603935/continuity-and-change-in-psychopathic-traits-among-school-aged-children-with-conduct-problems
#19
Vincent Bégin, Michèle Déry, Yann Le Corff
The three dimensions of psychopathic traits in childhood have been consistently associated with high levels of conduct problems among children. However, little is known about the continuity and change in psychopathic traits among children with conduct problems. This study aimed to examine the homotypic continuity and change of the three dimensions of psychopathic traits from 8 to 14 years old among 370 children (40.3% girls) who were identified at school as having conduct problems at study inception. Results supported the structural continuity of the tridimensional model of psychopathic traits and revealed that the callous-unemotional dimension is more stable than the two other dimensions...
January 2, 2019: Child Psychiatry and Human Development
https://read.qxmd.com/read/30543402/3d-scaffolds-based-on-conductive-polymers-for-biomedical-applications
#20
Nuria Alegret, Antonio Dominguez-Alfaro, David Mecerreyes
3D scaffolds appear to be a cost-effective ultimate answer for biomedical applications, facilitating rapid results while providing an environment similar to in vivo tissue. These biomaterials offer large surface areas for cell or biomaterial attachment, proliferation, biosensing and drug delivery applications. Among 3D scaffolds, the ones based on conjugated polymers (CPs) and natural non-conductive polymers arranged in a 3D architecture provide tridimensionality to cellular culture along with a high surface area for cell adherence and proliferation as well electrical conductivity for stimulation or sensing...
December 13, 2018: Biomacromolecules
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