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Leukemia expression

Wafaa M Rashed, Ali M Hammad, Anas M Saad, Kyrillus S Shohdy
Several studies detected abnormal mi-RNAs expression levels in childhood Acute Lymphoblastic Leukemia (ALL) with potential diagnostic value. We conducted a systematic search on certain microRNAs in childhood ALL. We included 17 studies with a total of 928 ALL children and 307 controls. Ten studies provided miRNAs expression levels and seven provided frequency data. Sensitivity and specificity of a single miRNA ranged from 46.55% to 100% and from 71.8% to 100%, respectively. The highest diagnostic odds ratio (DOR) was for the diagnostic panel (miR-128a and miR-223) reaching 546 [95% CI: 73...
April 2019: Critical Reviews in Oncology/hematology
Giulia Daniele, Alberto L'Abbate, Antonella Turchiano, Orazio Palumbo, Massimo Carella, Crocifissa Lo Cunsolo, Paolo Iuzzolino, Angelo Lonoce, María Hernández-Sánchez, Carla Minoia, Patrizia Leone, Jesus Maria Hernandez-Rivas, Clelia Tiziana Storlazzi
The identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). Nevertheless, the identification of novel additional molecular alterations is of great interest, opening to new prognostic and therapeutic strategies for such heterogeneous hematological disease. We here describe a patient affected by CLL with a mutated IGHV status, showing a balanced t(1;3)(q23.1;q21.3) translocation and a der(18)t(1;18)(q24.2;p11.32), accompanying the recurrent 13q14 heterozygous deletion in all analyzed cells at onset...
March 15, 2019: Clinical and Experimental Medicine
Ramin Radpour, Carsten Riether, Cedric Simillion, Sabine Höpner, Rémy Bruggmann, Adrian F Ochsenbein
CD8+ T cell immunosurveillance is crucial in solid tumors and T cell dysfunction leads to tumor progression. In contrast, the role of CD8+ T cells in the control of leukemia is less clear. We characterized the molecular signature of leukemia stem/progenitor cells (LSPCs) and paired CD8+ T cells in patients with acute myeloid leukemia (AML). Epigenetic alterations via histone deacetylation reduced the expression of immune-related genes in bone marrow (BM)-infiltrating CD8+ T cells. Surprisingly, a silenced gene expression pattern in CD8+ T cells significantly correlated with an improved prognosis...
March 15, 2019: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Zengqian Hui, Zhao Liu, Aili He, Yinxia Chen, Pengyu Zhang, Bo Lei, Huan Yao, Yong Yu, Rui Liang, Zhanning Li, Wanggang Zhang
Understanding the mechanisms of malignancy in acute myeloid leukemia (AML) cell is important for the targeted treatment and drug development. We found that visfatin, a 52-kDa adipokine, can positively regulate the proliferation of AML cells. Targeted inhibition of visfatin via its specific siRNAs or inhibitor can suppress the proliferation of AML cells. Further, knockdown of visfatin can increase the doxorubicin (Dox) and cisplatin (CDDP) sensitivity of AML cells. Among the tested six cytokines, si-visfatin can decrease the expression of interleukin-17 (IL-17)...
March 12, 2019: European Journal of Pharmacology
Zachariah Payne Sellers, Lukasz Bolkun, Janusz Kloczko, Marzena Liliana Wojtaszewska, Krzysztof Lewandowski, Marcin Moniuszko, Mariusz Z Ratajczak, Gabriela Schneider
BACKGROUND: The delta-like non-canonical Notch ligand 1 (DLK1)-maternally expressed 3(MEG3) locus (DLK1-MEG3 locus) plays a critical role in the maintenance and differentiation of hematopoietic stem cells. Accumulating evidence implicates the imprinted genes from this locus, DLK1 and MEG3, in the development and progression of acute myeloid leukemia (AML). However, the contribution of this locus to the treatment response of patients and their survival is unknown. METHODS: DNA methylation of select CG dinucleotide-containing amplicons (CpG sites) within the DLK1-MEG3 locus and within differentially methylated regions of other imprinted loci was assessed in the mononuclear cells of 45 AML patients by combined bisulfite restriction analysis...
March 15, 2019: Clinical Epigenetics
Feng Zhang, Jiajia Li, Junfeng Zhu, Lin Liu, Kai Zhu, Shuang Cheng, RuDi Lv, Pingping Zhang
BACKGROUND: The present study aimed to investigate the underlying role of interferon-regulatory factor 2 (IRF2)-inositol polyphosphate-4-phosphatase, type-II (INPP4B) axis in the regulation of autophagy in acute myeloid leukemia (AML) cells. METHODS: Quantitative real time PCR (QRT-PCR) and western blot were performed to determine the expression levels of IRF2, INPP4B and autophagy-related markers in AML cell lines. Autophagy was assessed by elevated Beclin-1 expression, the conversion of light chain 3 (LC3)-I to LC3-II, downregulated p62 expression and green fluorescent protein (GFP)-LC3 puncta formation...
March 15, 2019: Biological Research
Insha Ullah, Sudhir Paul, Zhenjie Hong, You-Gan Wang
MOTIVATION: Under two biologically different conditions, we are often interested in identifying differentially expressed genes. It is usually the case that the assumption of equal variances on the two groups is violated for many genes where a large number of them are required to be filtered or ranked. In these cases, exact tests are unavailable and the Welch's approximate test is most reliable one. The Welch's test involves two layers of approximations: approximating the distribution of the statistic by a t-distribution, which in turn depends on an approximate degrees of freedom...
March 15, 2019: Bioinformatics
Jannick Theobald, Mohamed A Abu El Maaty, Nico Kusterer, Bernhard Wetterauer, Michael Wink, Xinlai Cheng, Stefan Wölfl
Organ-on-chip platforms provide models that allow the representation of human physiological processes in cell-based miniaturized systems. Potential pre-clinical applications include drug testing and toxicity studies. Here we describe the use of a multi-compartment micro-fluidic chip to recapitulate hepatic vitamin D metabolism (vitamin D to 25-hydroxyvitamin D) and renal bio-activation (25-hydroxyvitamin D to 1,25-dihydroxyvitamin D) in humans. In contrast to cultivation in conventional tissue culture settings, on-chip cultivation of HepG2 and RPTEC cells in interconnected chambers, used to mimic the liver and kidneys, respectively, resulted in the enhanced expression of vitamin D metabolizing enzymes (CYP2R1, CYP27B1 and CYP24A1)...
March 15, 2019: Scientific Reports
Stefano Casola, Laura Perucho, Claudio Tripodo, Paola Sindaco, Maurilio Ponzoni, Fabio Facchetti
Surface expression of a functional B cell antigen receptor (BCR) is essential for the survival and proliferation of mature B cells. Most types of B-cell lymphoproliferative disorders retain surface BCR expression, including B-cell non-Hodgkin lymphomas (B-NHL) and chronic lymphocytic leukemia (CLL). Targeting BCR effectors in B-NHL cell lines in vitro has indicated that this signaling axis is crucial for malignant B cell growth. This has led to the development of inhibitors of BCR signaling, which are currently used for the treatment of CLL and several B-NHL subtypes...
March 2019: Immunological Reviews
Alex Pezzotta, Mara Mazzola, Marco Spreafico, Anna Marozzi, Anna Pistocchi
The genes of the cohesin complex exert different functions, ranging from the adhesion of sister chromatids during the cell cycle, DNA repair, gene expression and chromatin architecture remodeling. In recent years, the improvement of DNA sequencing technologies allows the identification of cohesin mutations in different tumors such as acute myeloid leukemia (AML), acute megakaryoblastic leukemia (AMKL), and myelodysplastic syndromes (MDS). However, the role of cohesin dysfunction in cancer insurgence remains elusive...
2019: Frontiers in Cell and Developmental Biology
Femke M Hormann, Alex Q Hoogkamer, H Berna Beverloo, Aurélie Boeree, Ilse Dingjan, Moniek M Wattel, Ronald W Stam, Gabriele Escherich, Rob Pieters, Monique L den Boer, Judith M Boer
No abstract text is available yet for this article.
March 14, 2019: Haematologica
Inez Johanna, Trudy Straetemans, Sabine Heijhuurs, Tineke Aarts-Riemens, Håkan Norell, Laura Bongiovanni, Alain de Bruin, Zsolt Sebestyen, Jürgen Kuball
BACKGROUND: γ9δ2T cells, which express Vγ9 and Vδ2 chains of the T cell receptor (TCR), mediate cancer immune surveillance by sensing early metabolic changes in malignant leukemic blast and not their healthy hematopoietic stem counterparts via the γ9δ2TCR targeting joined conformational and spatial changes of CD277 at the cell membrane (CD277J). This concept led to the development of next generation CAR-T cells, so-called TEGs: αβT cells Engineered to express a defined γδTCR...
March 12, 2019: Journal for Immunotherapy of Cancer
Alexander V Lavrov, Ekaterina Yu Chelysheva, Elmira P Adilgereeva, Oleg A Shukhov, Svetlana A Smirnikhina, Konstantin S Kochergin-Nikitsky, Valentina D Yakushina, Grigory A Tsaur, Sergey V Mordanov, Anna G Turkina, Sergey I Kutsev
BACKGROUND: Approximately 5-20% of chronic myeloid leukemia (CML) patients demonstrate primary resistance or intolerance to imatinib. None of the existing predictive scores gives a good prognosis of TKI efficacy. Gene polymorphisms, expression and microRNAs are known to be involved in the pathogenesis of TKI resistance in CML. The aim of our study is to find new molecular markers of TKI therapy efficacy in CML patients. METHODS: Newly diagnosed patients with Ph+ CML in chronic phase were included in this study...
March 13, 2019: BMC Medical Genomics
Megha Sravani Bondada, Yongxiu Yao, Venugopal Nair
MicroRNAs (miRNAs) are small noncoding RNAs that fine-tune the responses of the cell by modulating the cell transcriptome and gene expression. MicroRNA 155 (miR-155) is a conserved multifunctional miRNA involved in multiple roles including the modulation of the immune responses. When deregulated, miR-155 can also contribute to cancer as has been demonstrated in several human malignancies such as diffuse large B cell lymphoma, chronic lymphocytic leukemia, as well as in Epstein⁻Barr virus (EBV)-induced B cell transformation...
March 13, 2019: Non-Coding RNA
Leila Hadadi, Morteza Hafezi, Ali Akbar Amirzargar, Ramazan Ali Sharifian, Saeid Abediankenari, Hossein Asgarian-Omran
BACKGROUND: In this study, the expression pattern of NKp30 and T cell immunoglobulin and mucin domain-containing molecule-3 (Tim-3), as candidates for activating and inhibitory receptors of NK cells, were evaluated in patients with chronic lymphocytic leukemia (CLL). PATIENTS AND METHODS: 24 CLL patients and 19 healthy controls were enrolled. Fresh peripheral blood was collected from all subjects and stained with fluorochrome-conjugated antibodies. The frequency of CD56+/CD3-/NKp30+ and CD56+/CD3-/Tim-3+ cells was determined by multicolor flow cytometry...
March 14, 2019: Oncology Research and Treatment
Jun-Jie Lei, Hui-Qing Li, Zhi-Huai Mo, Ke-Jia Liu, Ling-Juan Zhu, Chun-Yi Li, Wen-Li Chen, Lei Zhang
The effective therapeutic approach of cerebral infarction is limited because of its underlying complexity. Recently, multiple long noncoding RNAs (lncRNAs) have been identified in the pathogenesis of cerebral infarction. Here, the current study aims to explore the interaction among lncRNA cyclin-dependent kinase inhibitor-2B-antisense RNA 1 (CDKN2B-AS1), transcription factor B-cell lymphoma/leukemia 11A (BCL11A), and MAPKK kinase kinase 1 (MAP4K1) and further investigate whether they affect cerebral infarction progression...
March 14, 2019: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Arezoo Rahimian, Majid Mahdavi, Reza Rahbarghazi, Hojjatollah Nozad Charoudeh
OBJECTIVE: Many experiments have revealed the anti-tumor activity of spiro-quinazolinone derivatives on different cell types. Exposing KG1-a cells to N-(4- tert- butyl- 4'- oxo- 1'H- spiro [cyclohexane- 1, 2'- quinazoline]- 3'(4'H)- yl)- 4- methyl benzenesulfonamide (4t-CHQ), as an active sub-component of spiro-quinazolinone benzenesulfonamides, the experiment investigated the possible mechanisms that manifest the role of 4t-CHQ in leukemic KG1-a progenitor cells. Mechanistically, the inhibitory effects of 4t-CHQ on KG1-a cells emerges from its madulating function on expression of Bax/Bcl2 and suvinin proteins...
March 13, 2019: Anti-cancer Agents in Medicinal Chemistry
Hacer B Afacan-Öztürk, Mesude Falay, Murat Albayrak, Abdulkerim Yıldız, Ҫiğdem Pala Öztürk, Senem Maral, Gülsüm Özet
BACKGROUND: Immunophenotyping has a central role in CLL. However, CLL is a very heterogenous disease, both morphologically and immunophenotypically; thus, its diagnosis may prove a challenge. We investigated CD81 ex-pression in the differential diagnosis of CLL and MCL. METHODS: We retrospectively examined CD81 expression with 8 color Multiparameter Flow cytometry devices in 101 CLL and 19 MCL cases. RESULTS: We found negative CD81 expression in CLL cases whereas it was positive in MCL cases...
March 1, 2019: Clinical Laboratory
Mohammad Ahmadvand, Mahsa Eskandari, Golnaz Khakpour, Hossein Pashaiefar, Saba Manoochehrabadi, Marjan Yaghmaie, Mostafa Montazer-Zohour, Anoosh Naghavi
<i>Background:</i> Chronic lymphocytic leukemia (CLL) is a type of malignancy in which the bone marrow makes too many lymphocytes. MicroRNAs (miRNAs) are endogenous short (~22-nucleotides) non-protein-coding regulatory RNA molecules with key roles in cellular and molecular processes linked to different cancers including CLL. Re-cently, some investigations have demonstrated that miR-125a downregulation is correlated with the expression of P53, NRG1 and ERBB2. <i>Methods:</i> In this study, samples including 38 patients with CLL and 25 healthy individuals were collected...
March 1, 2019: Clinical Laboratory
Erica Giuliani, Maria Giovanna Desimio, Margherita Doria
The hexamethylene bisacetamide (HMBA) anticancer drug was dismissed due to limited efficacy in leukemic patients but it may re-enter into the clinics in HIV-1 eradication strategies because of its recently disclosed capacity to reactivate latent virus. Here, we investigated the impact of HMBA on the cytotoxicity of natural killer (NK) cells against acute T lymphoblastic leukemia (T-ALL) cells or HIV-1-infected T cells that exit from latency. We show that in T-ALL cells HMBA upmodulated MICB and ULBP2 ligands for the NKG2D activating receptor...
March 13, 2019: Scientific Reports
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