Keywords Clostridium butyricum;Fermen...

Clostridium butyricum;Fermentation
Rongrong Li, Di Jiang, Mingli Zheng, Pengjiao Tian, Menghu Zheng, Chuncheng Xu
This study was conducted to examine the effects of Lactobacillus plantarum (LP) and sucrose (S) on clostridial community dynamics and correlation between clostridia and other bacteria in alfalfa silage during ensiling. Fresh alfalfa was directly ensiled without (CK) or with additives (LP, S, LP + S) for 7, 14, 28 and 56 days. Clostridial and bacterial communities were evaluated by next-generation sequencing. Severe clostridial fermentation occurred in CK, as evidenced by the high contents of butyric acid, ammonia nitrogen, and clostridia counts, whereas all additives, particularly LP + S, decreased silage pH and restrained clostridial fermentation...
October 20, 2020: Scientific Reports
Junhua Yun, Hossain M Zabed, Yufei Zhang, Amreesh Parvez, Guoyan Zhang, Xianghui Qi
The necessity of costly co-enzyme B12 for the activity of glycerol dehydratase (GDHt) is considered as a major bottleneck in sustainable bioproduction of 1,3-propanediol (1,3-PD) from glycerol. Here, an E. coil Rosetta-dhaB1-dhaB2 strain was constructed by overexpressing a B12 -independent GDHt (dhaB1) and its activating factor (dhaB2) from Clostridium butyricum. Subsequently, it was used in designing a co-culture with E. coli BL21-dhaT that overexpressed 1,3-PD oxidoreductase (dhaT), to produce 1,3-PD during co-fermentation of glycerol and glucose...
October 6, 2020: Bioresource Technology
Di-Wei Zheng, Run-Qing Li, Jia-Xin An, Tian-Qiu Xie, Zi-Yi Han, Rui Xu, Yu Fang, Xian-Zheng Zhang
While microbial-based therapy has been considered as an effective strategy for treating diseases such as colon cancer, its safety remains the biggest challenge. Here, probiotics and prebiotics, which possess ideal biocompatibility and are extensively used as additives in food and pharmaceutical products, are combined to construct a safe microbiota-modulating material. Through the host-guest chemistry between commercial Clostridium butyricum and chemically modified prebiotic dextran, prebiotics-encapsulated probiotic spores (spores-dex) are prepared...
October 1, 2020: Advanced Materials
Diana N Obanda, Claudia Husseneder, Anne M Raggio, Ryan Page, Brian Marx, Rhett W Stout, Justin Guice, Diana Coulon, Michael J Keenan
OBJECTIVES: Gut microbiota profiles contribute to differences in obesity phenotype. We examined the abundance of the species Clostridium butyricum in relation to obesity phenotype. METHODS: In outbred Sprague -Dawley rats we examined effects of dietary fat, resistant starch (RS), and a microbiota transplant on obesity phenotype. Using targeted qPCR, we examined the abundance of total gut bacteria and C. butyricum in relation to the propensity of obesity prone and obesity resistant rats to accumulate abdominal fat...
June 15, 2020: Nutrition
Ai-Hui Zhang, Kai-Yi Zhu, Xiao-Yan Zhuang, Lang-Xing Liao, Shi-Yang Huang, Chuan-Yi Yao, Bai-Shan Fang
With the aggravation of environmental pollution and energy crisis, the sustainable microbial fermentation process of converting glycerol to 1,3-propanediol (1,3-PDO) has become an attractive alternative. However, the difficulty in the online measurement of glycerol and 1,3-PDO creates a barrier to the fermentation process and then leads to the residual glycerol and therefore, its wastage. Thus, in the present study, the four-input artificial neural network (ANN) model was developed successfully to predict the concentration of glycerol, 1,3-PDO, and biomass with high accuracy...
July 17, 2020: Biotechnology and Bioengineering
Young-Bo Sim, Ju-Hyeong Jung, Jong-Hun Park, Péter Bakonyi, Sang-Hyoun Kim
This study examined the effect of shear velocity on biohydrogen producing dynamic membrane bioreactor (DMBR) containing 50 µm polyester mesh as supporting material. Increase of shear velocity up to 6.75 m/h enhanced hydrogen production performance as well as biomass retention in both suspended and attached forms, while wash-out was found at a shear velocity of 11.69 m/h. The highest average HPR, HY, suspended biomass, and attached biomass were 26.56 ± 1.49 L/L-d, 1.78 ± 0.10 mol H2 /mol glucoseadded , 9...
March 30, 2020: Bioresource Technology
Suhong Li, Xiushan Zhang, Yan Li, Lu Tao, Tuoping Li
Clostridium butyricum, an anaerobic spore-forming bacillus, is a common human and animal gut commensal bacterium. Spore is an important structure for C. butyricum to tolerate environmental stress. However, it is not easy to form in common fermentation process of C. butyricum. In this study, the parameters for optimizing the spore formation of C. butyricum NN-2 were defined. The results showed that the pH value was a crucial factor that significantly affected the spore formation of C. butyricum NN-2. Down-regulation steps of pH value from 6...
July 2020: Archives of Microbiology
Meining Xing, Yifeng Wei, Gaoqun Hua, Mengya Li, Ankanahalli N Nanjaraj Urs, Feifei Wang, Yiling Hu, Weixiang Zhai, Yangping Liu, Ee Lui Ang, Huimin Zhao, Yan Zhang
Aminoethylsulfonate (taurine) is widespread in the environment, and highly abundant in the human body. Taurine and other aliphatic sulfonates serve as sulfur sources for diverse aerobic bacteria, which carry out cleavage of the inert sulfonate C-S bond through various O2 -dependent mechanisms. Taurine also serves as a sulfur source for certain strict anaerobic fermenting bacteria. However, the mechanism of C-S cleavage by these bacteria has long been a mystery. Here we report the biochemical characterization of an anaerobic pathway for taurine sulfur assimilation in a strain of Clostridium butyricum from the human gut...
July 26, 2019: Biochemical Journal
Yanan Yin, Jianlong Wang
This study explored the mechanisms of the enhanced hydrogen production from macroalgae by Fe2+ supplementation. Highest hydrogen yield of 19.47 mL/g VSadded was achieved at Fe2+ supplementation of 400 mg/L, which was 6.25 times of the control test. In depth analysis of substrate degradation, microbial distribution and metabolites formation was conducted. The results showed that Fe2+ -supplemented group was dominated by Clostridium butyricum (67.2%) and Ruminococcus gnavus (24.2%), which stimulated hydrogen generation and volatile organic acids accumulation...
November 2019: Bioresource Technology
Rahul Mangayil, Elena Efimova, Jukka Konttinen, Ville Santala
Crude glycerol is an excellent carbon source for bacterial production systems. Bacterial fermentation often generates by-products that can offer an additional carbon pool to improve the product profile for optimal valorization. In this study, the properties of two phylogenetically distinct bacteria, Acinetobacter baylyi ADP1 and Clostridium butyricum, were coupled in a one-pot batch process to co-produce 1,3 propanediol (PDO) and long-chain alkyl esters (wax esters, WEs) from crude glycerol. In the process, A...
July 11, 2019: New Biotechnology
Diana Obanda, Ryan Page, Justin Guice, Anne Raggio, Diana Coulon, Michael Keenan
Objectives: Gut microbiota profiles contribute to differences in obesity phenotype. The contribution of specific bacterial species has not been shown. In two sequential studies, we examined the abundance of total bacteria, and the species Clostridium butyricum in relation to the propensity of outbred Sprague-Dawley CD rats to accumulate abdominal fat. As a probiotic, C. butyricum is associated with enhanced butyrate production, reduced inflammation and prevention of diet induced weight gain...
June 2019: Current Developments in Nutrition
Jeong-Hoon Park, Do-Hyung Kim, Han-Shin Kim, George F Wells, Hee-Deung Park
In this work, the effects of granular activated carbon (GAC) supplementation on hydrogen fermentation were investigated in Clostridium butyricum. Maximum hydrogen production rate and ultimate hydrogen volume increased up to 1.7 and 1.9 fold, respectively, with 1 g/L GAC supplementation. Indicators of stable hydrogen production, such as acetate and butyrate formation increased with increasing GAC concentration. To identify the factors for hydrogen production enhancement, transcriptome analysis was conducted...
February 25, 2019: Bioresource Technology
Anna Detman, Damian Mielecki, Aleksandra Chojnacka, Agnieszka Salamon, Mieczysław K Błaszczyk, Anna Sikora
BACKGROUND: Interactions between microorganisms during specific steps of anaerobic digestion determine metabolic pathways in bioreactors and consequently the efficiency of fermentation processes. This study focuses on conversion of lactate and acetate to butyrate by bacteria of dark fermentation. The recently recognized flavin-based electron bifurcation as a mode of energy coupling by anaerobes increases our knowledge of anaerobic lactate oxidation and butyrate formation. RESULTS: Microbial communities from dark fermentation bioreactors or pure culture of Clostridium butyricum are able to convert lactate and acetate to butyrate in batch experiments...
February 13, 2019: Microbial Cell Factories
Haidong Li, Xiangli Tian, Kun Zhao, Wenwen Jiang, Shuanglin Dong
A 42-day feeding trial was conducted to evaluate the effects of diet supplemented with various additives from Clostridium butyricum (fermentation supernatant, FS; live cells, LC; cell-free extract, CE; spray-dried spores, DS; mixture of live cells and supernatant, LCS) on the growth, intestinal morphology, disease resistance, immune gene expression and mTOR signaling-related gene expression in Litopenaeus vannamai. The feeding trial showed that the final weight and specific growth rate of the shrimp were improved significantly while the feed conversion ratio were reduced significantly in LC, CE, DS and LCS groups compared to the control...
December 29, 2018: Fish & Shellfish Immunology
Ibukun Ogunade, Hank Schweickart, Kenneth Andries, Jerusha Lay, James Adeyemi
To identify differences in rumen function as a result of feeding monensin to beef cattle, rumen fluid metagenomics and metabolomics analyses were used to evaluate the functional attributes and metabolites of rumen microbiota in beef steers fed no or 200 mg/d of monensin. Eight rumen-fistulated steers were used in the study for a period of 53 days. Rumen fluid samples were collected on the last day of the experiment. Monensin increased the relative abundance of Selenomonas sp. ND2010, Prevotella dentalis , Hallella seregens , Parabacteroides distasonis , Propionispira raffinosivorans , and Prevotella brevis , but reduced the relative abundance of Robinsoniella sp...
November 17, 2018: Animals: An Open Access Journal From MDPI
Li-Wen Su, Yeong-Hsiang Cheng, Felix Shih-Hsiang Hsiao, Jin-Cheng Han, Yu-Hsiang Yu
Soybean meal is the main vegetable protein source in animal feed. Soybean meal contains several anti-nutritional factors, which directly affect digestion and absorption of soy protein, thereby reducing growth performance and value in animals. Fermented soybean meal is rich in probiotics and functional metabolites, which facilitates soybean protein digestion, absorption and utilization in piglets. However, the mixed solid-state fermentation (SSF) conditions of soybean meal remain to be optimized. In this study, we investigated the optimal parameters for SSF of soybean meal by Lactobacillus species and Clostridium butyricum ...
2018: Polish Journal of Microbiology
Yeseul Shin, Jayoung Paek, Ah Young Son, Hongik Kim, Joong-Ki Kook, Woon Kee Paek, Young Hyo Chang
Strain YH-tan31T is a Gram-stain-positive, obligately anaerobic, spore-forming and mesophilic bacterium. The cells are rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium oryzae DSM 28571T (97 % sequence identity), followed by Clostridium cellulovorans DSM 3052T (92.4 %) and Clostridium butyricum KCTC 5387T (92.1 %). The G+C content of the isolate was 30...
October 25, 2018: International Journal of Systematic and Evolutionary Microbiology
Surajit De Mandal, Sambanduram Samarjit Singh, Rajendra Bose Muthukumaran, Kawl Thanzami, Vinod Kumar, Nachimuthu Senthil Kumar
Fermented pork fat (sa-um) is traditionally and extensively consumed in Northeast Indian region for several decades. However, no scientific reports are available regarding its nutritional value as well as its potential health risks. The objective of this work was essentially the characterization of sa-um using a polyphasic approach, viz., physicochemical, electrospray ionization-mass spectrometry (ESI+ -MS) and metagenomic analysis in order to gain an understanding of the nutrient contents and microbial population diversity...
October 8, 2018: AMB Express
Jin-Jie Zhou, Jun-Tao Shen, Xiao-Li Wang, Ya-Qin Sun, Zhi-Long Xiu
Microbial consortium is an alternative for bioconversion of crude glycerol to value-added products whereas concerns about the process stability in long-term operation existed. The aim of this study is to evaluate the feasibility of using an anaerobic microbial consortium as inoculum for continuous conversion of crude glycerol to 1,3-propanediol (1,3-PDO). Performances of continuous fermentations with the consortium inoculum were evaluated under different dilution rates and glycerol feed concentrations. The highest 1,3-PDO production of 57...
October 2018: Applied Microbiology and Biotechnology
Jong-Hun Park, Young-Bo Sim, Gopalakrishnan Kumar, Parthiban Anburajan, Jeong-Hoon Park, Hee-Deung Park, Sang-Hyoun Kim
This study investigated the kinetic parameters of high-rate continuous performance and biofilm layer formation in a H2 -producing dynamic membrane bioreactor, composed of a continuously stirred tank reactor along with an external module containing polyester mesh with a pore size of 100 µm. A maximum H2 production rate of 48.9 L/L-day and hydrogen yield of 2.8 mol/mol glucoseadded were attained at a hydraulic retention time of 3 h. The maximum specific growth rate and Monod constant were estimated as 14...
August 2018: Bioresource Technology
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