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Clostridium butyricum;Fermentation

Anna Detman, Damian Mielecki, Aleksandra Chojnacka, Agnieszka Salamon, Mieczysław K Błaszczyk, Anna Sikora
BACKGROUND: Interactions between microorganisms during specific steps of anaerobic digestion determine metabolic pathways in bioreactors and consequently the efficiency of fermentation processes. This study focuses on conversion of lactate and acetate to butyrate by bacteria of dark fermentation. The recently recognized flavin-based electron bifurcation as a mode of energy coupling by anaerobes increases our knowledge of anaerobic lactate oxidation and butyrate formation. RESULTS: Microbial communities from dark fermentation bioreactors or pure culture of Clostridium butyricum are able to convert lactate and acetate to butyrate in batch experiments...
February 13, 2019: Microbial Cell Factories
Haidong Li, Xiangli Tian, Kun Zhao, Wenwen Jiang, Shuanglin Dong
A 42-day feeding trial was conducted to evaluate the effects of diet supplemented with various additives from Clostridium butyricum (fermentation supernatant, FS; live cells, LC; cell-free extract, CE; spray-dried spores, DS; mixture of live cells and supernatant, LCS) on the growth, intestinal morphology, disease resistance, immune gene expression and mTOR signaling-related gene expression in Litopenaeus vannamai. The feeding trial showed that the final weight and specific growth rate of the shrimp were improved significantly while the feed conversion ratio were reduced significantly in LC, CE, DS and LCS groups compared to the control...
December 29, 2018: Fish & Shellfish Immunology
Ibukun Ogunade, Hank Schweickart, Kenneth Andries, Jerusha Lay, James Adeyemi
To identify differences in rumen function as a result of feeding monensin to beef cattle, rumen fluid metagenomics and metabolomics analyses were used to evaluate the functional attributes and metabolites of rumen microbiota in beef steers fed no or 200 mg/d of monensin. Eight rumen-fistulated steers were used in the study for a period of 53 days. Rumen fluid samples were collected on the last day of the experiment. Monensin increased the relative abundance of Selenomonas sp. ND2010, Prevotella dentalis , Hallella seregens , Parabacteroides distasonis , Propionispira raffinosivorans , and Prevotella brevis , but reduced the relative abundance of Robinsoniella sp...
November 17, 2018: Animals: An Open Access Journal From MDPI
Li-Wen Su, Yeong-Hsiang Cheng, Felix Shih-Hsiang Hsiao, Jin-Cheng Han, Yu-Hsiang Yu
Soybean meal is the main vegetable protein source in animal feed. Soybean meal contains several anti-nutritional factors, which directly affect digestion and absorption of soy protein, thereby reducing growth performance and value in animals. Fermented soybean meal is rich in probiotics and functional metabolites, which facilitates soybean protein digestion, absorption and utilization in piglets. However, the mixed solid-state fermentation (SSF) conditions of soybean meal remain to be optimized. In this study, we investigated the optimal parameters for SSF of soybean meal by Lactobacillus species and Clostridium butyricum ...
2018: Polish Journal of Microbiology
Yeseul Shin, Jayoung Paek, Ah Young Son, Hongik Kim, Joong-Ki Kook, Woon Kee Paek, Young Hyo Chang
Strain YH-tan31T is a Gram-stain-positive, obligately anaerobic, spore-forming and mesophilic bacterium. The cells are rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium oryzae DSM 28571T (97 % sequence identity), followed by Clostridium cellulovorans DSM 3052T (92.4 %) and Clostridium butyricum KCTC 5387T (92.1 %). The G+C content of the isolate was 30...
October 25, 2018: International Journal of Systematic and Evolutionary Microbiology
Surajit De Mandal, Sambanduram Samarjit Singh, Rajendra Bose Muthukumaran, Kawl Thanzami, Vinod Kumar, Nachimuthu Senthil Kumar
Fermented pork fat (sa-um) is traditionally and extensively consumed in Northeast Indian region for several decades. However, no scientific reports are available regarding its nutritional value as well as its potential health risks. The objective of this work was essentially the characterization of sa-um using a polyphasic approach, viz., physicochemical, electrospray ionization-mass spectrometry (ESI+ -MS) and metagenomic analysis in order to gain an understanding of the nutrient contents and microbial population diversity...
October 8, 2018: AMB Express
Jin-Jie Zhou, Jun-Tao Shen, Xiao-Li Wang, Ya-Qin Sun, Zhi-Long Xiu
Microbial consortium is an alternative for bioconversion of crude glycerol to value-added products whereas concerns about the process stability in long-term operation existed. The aim of this study is to evaluate the feasibility of using an anaerobic microbial consortium as inoculum for continuous conversion of crude glycerol to 1,3-propanediol (1,3-PDO). Performances of continuous fermentations with the consortium inoculum were evaluated under different dilution rates and glycerol feed concentrations. The highest 1,3-PDO production of 57...
October 2018: Applied Microbiology and Biotechnology
Jong-Hun Park, Young-Bo Sim, Gopalakrishnan Kumar, Parthiban Anburajan, Jeong-Hoon Park, Hee-Deung Park, Sang-Hyoun Kim
This study investigated the kinetic parameters of high-rate continuous performance and biofilm layer formation in a H2 -producing dynamic membrane bioreactor, composed of a continuously stirred tank reactor along with an external module containing polyester mesh with a pore size of 100 µm. A maximum H2 production rate of 48.9 L/L-day and hydrogen yield of 2.8 mol/mol glucoseadded were attained at a hydraulic retention time of 3 h. The maximum specific growth rate and Monod constant were estimated as 14...
August 2018: Bioresource Technology
Tiago Pinto, Luísa Gouveia, Joana Ortigueira, Ganesh D Saratale, Patrícia Moura
In this work, hydrogen (H2 ) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90...
August 2018: Journal of Bioscience and Bioengineering
Waraporn Apiwatanapiwat, Pilanee Vaithanomsat, Warunee Thanapase, Khanok Ratanakhanokchai, Akihiko Kosugi
Lignocellulosic biomass as co-substrate enhances the 1,3-propanediol (1,3-PD) production of anaerobic fermenters by increasing their conversion yield from glycerol. To improve 1,3-propanediol (1,3-PD) production by this efficient approach, Clostridium butyricum I5-42 was supplemented with lignocellulosic biomasses (starch free fiber (CPF) from cassava pulp and xylan) as co-substrates. The 1,3-PD production and growth of C. butyricum were considerably higher in glycerol plus CPF and xylan than in glycerol alone, whereas another major polysaccharide (cellulose co-substrate) failed to improve the 1,3-PD production...
June 2018: Journal of Bioscience and Bioengineering
Ai-Hui Zhang, Hao-Lin Liu, Shi-Yang Huang, You-Si Fu, Bai-Shan Fang
1,3-propanediol production by Clostridium butyricum is a low productivity process due to the long time seed cultivation and thus hinders its industrial scale production. In the present study, repeated batch fermentation coupled with activated carbon adsorption strategy was first established which conduced not only to saving the time of seed cultivation and enhancing the productivity, but also to reducing the costs for the seed cultivation to achieve the purpose of 1,3-propanediol continuous production. The concentration of 1,3-propanediol from first to fourth cycle was 42...
March 2018: Biotechnology and Bioengineering
Luis Miguel Serrano-Bermúdez, Andrés Fernando González Barrios, Costas D Maranas, Dolly Montoya
BACKGROUND: The increase in glycerol obtained as a byproduct of biodiesel has encouraged the production of new industrial products, such as 1,3-propanediol (PDO), using biotechnological transformation via bacteria like Clostridium butyricum. However, despite the increasing role of Clostridium butyricum as a bio-production platform, its metabolism remains poorly modeled. RESULTS: We reconstructed iCbu641, the first genome-scale metabolic (GSM) model of a PDO producer Clostridium strain, which included 641 genes, 365 enzymes, 891 reactions, and 701 metabolites...
June 1, 2017: BMC Systems Biology
Parthiban Anburajan, Jong-Hun Park, Periyasamy Sivagurunathan, Arivalagan Pugazhendhi, Gopalakrishnan Kumar, Chang-Su Choi, Sang-Hyoun Kim
This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pretreated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3±0.1 mol/mol hexoseadded, was achieved at 3 h HRT...
September 2017: Journal of Bioscience and Bioengineering
Jin-Jie Zhou, Jun-Tao Shen, Li-Li Jiang, Ya-Qin Sun, Ying Mu, Zhi-Long Xiu
Crude glycerol is an ideal feedstock for bioproduction of 1,3-propanediol (1,3-PDO) while pure culture always shows low substrate tolerance and limited productivity. In this study, an anaerobic microbial consortium for conversion of crude glycerol was selected and its 1,3-PDO production capacity was evaluated. The consortium was obtained from anaerobic activated sludge by 19 serial transfers and mainly consisted of 94.64% Clostridiaceae and 4.47% Peptostreptococcaceae. The consortium adapted well with high glycerol concentration of 120 g/L as well as wide substrate concentration fluctuation from 15 to 80 g/L, producing 60...
August 2017: Applied Microbiology and Biotechnology
Zhao Kang Tee, Jamaliah Md Jahim, Jian Ping Tan, Byung Hong Kim
Calcium carbonate was evaluated as a replacement for the base during the fermentation of glycerol by a highly productive strain of 1,3-propanediol (PDO), viz., Clostridium butyricum JKT37. Due to its high specific growth rate (µmax=0.53h(-1)), 40g/L of glycerol was completely converted into 19.6g/L of PDO in merely 7h of batch fermentation, leaving only acetate and butyrate as the by-products. The accumulation of these volatile fatty acids was circumvented with the addition of calcium carbonate as the pH neutraliser before the fermentation was inoculated...
June 2017: Bioresource Technology
Dan Jiang, Zhen Fang, Siew-Xian Chin, Xiao-Fei Tian, Tong-Chao Su
Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0...
June 2, 2016: Scientific Reports
Waraporn Apiwatanapiwat, Pilanee Vaithanomsat, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai, Akihiko Kosugi
To improve its 1,3-propanediol (1,3-PD) production, Clostridium butyricum was cultivated on glycerol medium supplemented with cassava pulp (CP). At small concentrations, the CP improved the 1,3-PD productivity of C. butyricum from (0.25±0.01)g/L/h (glycerol alone) to (0.43±0.02)g/L/h (glycerol+2g/L CP) after 24h fermentation.
July 20, 2016: Journal of Biotechnology
Chunjie Zhu, Baishan Fang, Shizhen Wang
The effects of culture conditions on the kinetic behavior of 1,3-propanediol (PD) fermentation were investigated with a kinetic model. First, with initial glycerol concentration (S0) increasing, μmax and PD inhibition increased. Glycerol assimilation was harder and a little glycerol was consumed on cell maintenance at high S0. Second, with yeast extract concentration increasing, PD inhibition decreased. However, μmax decreased and glycerol assimilation became harder. It seems that the stimulus effect of yeast extract resulted from decreased PD inhibition...
July 2016: Bioresource Technology
Matti S Kannisto, Rahul K Mangayil, Ankita Shrivastava-Bhattacharya, Brett I Pletschke, Matti T Karp, Ville P Santala
BACKGROUND: Pretreatment of lignocellulosic biomass can produce inhibitory compounds that are harmful for microorganisms used in the production of biofuels and other chemicals from lignocellulosic sugars. Selective inhibitor removal can be achieved with biodetoxification where microorganisms catabolize the inhibitors without consuming the sugars. We engineered the strictly aerobic Acinetobacter baylyi ADP1 for detoxification of lignocellulosic hydrolysates by removing the gene for glucose dehydrogenase, gcd, which catalyzes the first step in its glucose catabolism...
2015: Biotechnology for Biofuels
N Cassir, S Benamar, B La Scola
Clostridium butyricum, a strictly anaerobic spore-forming bacillus, is a common human and animal gut commensal bacterium, and is also frequently found in the environment. Whereas non-toxigenic strains are currently used as probiotics in Asia, other strains have been implicated in pathological conditions, such as botulism in infants or necrotizing enterocolitis in preterm neonates. In terms of the latter, within the same species, different strains have antagonist effects on the intestinal mucosa. In particular, short-chain fatty acids, which are products of carbohydrate fermentation, have a dose-dependent paradoxical effect...
January 2016: Clinical Microbiology and Infection
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