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Ross Kent, Neil Dixon
Since their discovery riboswitches have been attractive tools for the use-controlled regulation of gene expression in bacterial systems. Riboswitches facilitate small molecule mediated fine-tuning of protein expression, making these tools of great use to the synthetic biology community. However, the use of riboswitches is often restricted due to context dependent performance, and limited dynamic range. Here we report the drastic improvement of a previously developed orthogonal riboswitch, achieved through in vivo functional selection and optimisation of flanking coding and non-coding sequences...
March 21, 2019: ACS Synthetic Biology
G A Nugmanov, A Y Komkov, M V Saliutina, A A Minervina, Y B Lebedev, I Z Mamedov
Retroelements are considered as one of the important sources of genomic variability in modern humans. It is known that transposition activity of retroelements in germline cells generates new insertions in various genomic loci and sometimes results in genetic diseases. Retroelements activity in somatic cells is restricted by different cellular mechanisms; however, there is an evidence for it in some tissue types. Somatic insertions can trigger tumorigenesis or participate in normal functioning such as generation of neurons' plasticity...
January 2019: Molekuliarnaia Biologiia
Yuancheng Wang, Feng Wang, Sheng Xu, Riyuan Wang, Wenjing Chen, Kai Hou, Chi Tian, Fan Wang, Ping Zhao, Qingyou Xia
The multigene expression system is highly attractive to co-express multiple genes or multi-subunit complex-based genes for their functional studies, and in gene therapy and visual tracking of expressed proteins. However, the current multiple gene co-expression strategies usually suffer from severe inefficiency and unbalanced expression of multiple genes. Here, we report on an improved 2A self-cleaving peptide (2A)-based multigene expression system (2A-MGES), by introducing an optimized Kozak region (Ck) and altering the gene arrangement, both of which contributed to the efficient expression of two fluorescent protein genes in silkworm...
March 20, 2019: Molecular Genetics and Genomics: MGG
Xiaotuo Zhang, Yong Zhang, Taiyun Wang, Ziwei Li, Jinping Cheng, Haoran Ge, Qi Tang, Kun Chen, Li Liu, Chenyu Lu, Junqiang Guo, Binglian Zheng, Yun Zheng
Lariat is formed by excised intron, in which the 5' splice site joints with the branchpoint (BP) during splicing. Although lariat RNAs are usually degraded by DBR1 (RNA debranching enzyme 1), recent findings in animals showed that many lariat RNAs were widely detected under physiological conditions. In contrast, the features of BPs and to what extent lariat RNAs accumulate naturally are largely unexplored in plants. Here, we analyzed 948 RNA sequencing datasets to document plant BPs and lariat RNAs on a genome-wide scale...
March 20, 2019: Plant Cell
Zhongming Zhang, Yijing Zhang, Yan Wang, Shengchen Ding, Chenhui Wang, Li Gao, Alan Johnson, Baojian Xue
INTRODUCTION: Brain-derived neurotropic factor (BDNF) is expressed throughout the central nervous system and peripheral organs involved in the regulation of blood pressure, but the systemic effects of BDNF in the control of blood pressure are not well elucidated. MATERIALS AND METHODS: We utilized loxP flanked BDNF male mice to cross with nestin-Cre female mice to generate nerve system BDNF knockdown mice, nestin-BDNF (+/-), or injected Cre adenovirus into the subfornical organ to create subfornical organ BDNF knockdown mice...
January 2019: Journal of the Renin-angiotensin-aldosterone System: JRAAS
Jangampalli Adi Pradeepkiran, P Hemachandra Reddy
The purpose of our study is to identify phosphorylated tau (p-tau) inhibitors. P-tau has recently received great interest as a potential drug target in Alzheimer's disease (AD). The continuous failure of Aβ-targeted therapeutics recommends an alternative drug target to treat AD. There is increasing evidence and growing awareness of tau, which plays a central role in AD pathophysiology, including tangles formation, abnormal activation of phosphatases/kinases, leading p-tau aggregation in AD neurons. In the present study, we performed computational pharmacophore models, molecular docking, and simulation studies for p-tau in order to identify hyperphosphorylated sites...
March 19, 2019: Cells
Usman Bashir, Amanda Weeks, Jayant S Goda, Muhammad Siddique, Vicky Goh, Gary J Cook
PURPOSE: Treatment of metastatic colorectal cancer frequently includes antiangiogenic agents such as bevacizumab. Size measurements are inadequate to assess treatment response to these agents, and newer response assessment criteria are needed. We aimed to evaluate F-FDG PET-derived texture parameters in a preclinical colorectal cancer model as alternative metrics of response to treatment with bevacizumab. MATERIALS AND METHODS: Fourteen CD1 athymic mice injected in the flank with 5×106 LS174T cells (human colorectal carcinoma) were either untreated controls (n=7) or bevacizumab treated (n=7)...
March 18, 2019: Nuclear Medicine Communications
Sophie Bishop, Marie-Hélène Tremblay, Alexandra Gellé, W G Skene
With the aim of achieving reversible oxidation and color tuning, the effect of the central aromatic on the spectroscopic, electrochemical, and spectrochemical properties of a series of electrochromic azomethine triads was investigated. The absorption of the alkylated thiophene derivatives was blue-shifted relative to their unalkylated counterparts when the central aromatic was either a bi- or terthiophene. It was further found that the alkylated thiophene derivatives had larger Stokes shifts than their unsubstituted counterparts...
March 20, 2019: Journal of Physical Chemistry. A
Talha Sarigoz, Yusuf Sevim, Inanc Samil Sarici, Omer Topuz, Tamer Ertan
BACKGROUND: The clinical approach to back/flank wounds has evolved over the years. The aim of this study was to discuss the potential of computed tomography tractography in patients with a stab wound to the back or flank. METHODS: A total of 25 stable patients with stab wounds confined to the back/flank region were enrolled in this retrospective study. After initial resuscitation and physical examination, tractography was performed at the site of the stab wound...
March 2019: Turkish Journal of Trauma & Emergency Surgery: TJTES
A F Nogueira, F Langeani, A L Netto-Ferreira
Hemiodus bimaculatus sp. nov., is described from tributaries of the Rio Juruena and Rio Teles Pires in the upper Rio Tapajós basin. The new species is diagnosed from most congeners, except Hemiodus jatuarana, by having a conspicuous circular or horizontally elongate dark blotch on the caudal peduncle (v. inconspicuous in H. iratapuru and absent in the other species). The new species differs from H. jatuarana by having a round midlateral spot on the flank (v. absent in H. jatuarana), 98-121 perforated scales in the lateral line (v...
March 20, 2019: Journal of Fish Biology
Rachel S Plummer, Catherine A Forestell
The goal of this study was to investigate how exposure to commercials featuring thin or plus-size models affects women's implicit cognitive responses to food. One hundred sixteen college-age women watched a neutral documentary that contained a commercial depicting either a thin woman (n = 39), a plus-size woman (n = 38), or content with no human actors (n = 39). After the documentary, women completed the Flanker task to measure their implicit attention to foods, the Affect Misattribution Procedure (AMP) to measure their implicit evaluation of food, and a measure of state body dissatisfaction...
March 16, 2019: Appetite
A Escudeiro, F Adega, T J Robinson, J S Heslop-Harrison, R Chaves
Repetitive satellite DNA (satDNA) sequences are abundant in eukaryote genomes, with a structural and functional role in centromeric function. We analysed the nucleotide sequence and chromosomal location of the five known cattle (Bos taurus) satDNA families in seven species from the tribe Tragelaphini (Bovinae subfamily). One of the families (SAT1.723) was present at the chromosomes' centromeres of the Tragelaphini species, as well in two more distantly related bovid species, Ovis aries and Capra hircus. Analysis of the interaction of SAT1...
March 19, 2019: Genome Biology and Evolution
Ashleigh M Maxcey, Laura Janakiefski, Emma Megla, Madison Smerdell, Samantha Stallkamp
A large body of literature agrees that accessing a target memory appears to trigger a difference-of-Gaussian memory activation pulse under which the target representation is activated and categorically flanking items are suppressed and forgotten. The nature of the underlying forgetting mechanism is far from settled, with support for several theories of forgetting. Here we argue the debate is partly fueled by different forgetting mechanisms underlying the forgetting of different memoranda. We capitalized on the unique aspect of the recognition-induced forgetting paradigm to test forgetting of both pictures and words in identical recognition-practice and restudy tasks...
March 18, 2019: Psychonomic Bulletin & Review
Lisa Valentina Eberhardt, Anke Huckauf
Crowding refers to the phenomenon of reduced recognition performance for peripherally presented targets that are flanked by similar stimuli. Crowding is known to vary with lateral distances (i.e., effects of target eccentricity and inter-character spacing). In the present experiment, we examined how crowding is affected by the distance of the stimuli in depth for natural viewing, i.e., for binocular observation of a real depth presentation. Superimposing the displays of two orthogonally arranged screens with a half-transparent mirror created real-depth presentation...
March 18, 2019: Attention, Perception & Psychophysics
Jae Uk Chong, Chang Moo Kang
PURPOSE: Laparoscopic approaches to enucleation of the pancreas have been frequently described. Here we present a case of robotic single-site plus one port pancreas enucleation. To our knowledge, this enucleation surgical technique is the first to be reported in the medical literature. METHODS: A 46-year-old male patient without previous medical or surgical history was incidentally diagnosed with a pancreatic mass during evaluation of intermittent right flank pain...
March 18, 2019: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
Eleni Liapis, Maxime Bour, Pauline Triponney, Thomas Jové, Jean-Ralph Zahar, Benoît Valot, Katy Jeannot, Patrick Plésiat
A novel carbapenem-hydrolyzing beta-lactamase, called IMP-63, was identified in three clonally distinct strains of Pseudomonas aeruginosa and two strains of Pseudomonas putida isolated within a 4 year timeframe in three French hospitals. The bla IMP-63 gene that encodes this carbapenemase turned out to be located in the variable region of four integrons (In 1297 , In 1574 , In 1573 , and In 1572 ) and to coexist with novel or rare gene cassettes ( fosM , gcu170 , gcuF1 ) and insertion elements (IS Psp7v , IS Pa16v )...
2019: Frontiers in Microbiology
Shaoya Li, Jingying Li, Yubing He, Meilian Xu, Jiahui Zhang, Wenming Du, Yunde Zhao, Lanqin Xia
One of the main obstacles to gene replacement in plants is efficient delivery of a donor repair template (DRT) into the nucleus for homology-directed DNA repair (HDR) of double-stranded DNA breaks. Production of RNA templates in vivo for transcript-templated HDR (TT-HDR) could overcome this problem, but primary transcripts are often processed and transported to the cytosol, rendering them unavailable for HDR. We show that coupling CRISPR-Cpf1 (CRISPR from Prevotella and Francisella 1) to a CRISPR RNA (crRNA) array flanked with ribozymes, along with a DRT flanked with either ribozymes or crRNA targets, produces primary transcripts that self-process to release the crRNAs and DRT inside the nucleus...
March 18, 2019: Nature Biotechnology
Wei-Wei Chen, Qi Yang, Xiao-Yao Li, Xiao-Lei Shi, Na Pu, Guo-Tao Lu, Zhi-Hui Tong, Jian-Min Chen, Wei-Qin Li
BACKGROUND: Hypertriglyceridemia (HTG) is one of the most common etiologies of acute pancreatitis (AP). Variants in five genes involved in the regulation of plasma lipid metabolism, namely LPL, APOA5, APOC2, GPIHBP1 and LMF1, have been frequently reported to cause or predispose to HTG. METHODS: A Han Chinese patient with HTG-induced AP was assessed for genetic variants by Sanger sequencing of the entire coding and flanking sequences of the above five genes. RESULTS: The patient was a 32-year-old man with severe obesity (Body Mass Index = 35) and heavy smoking (ten cigarettes per day for more than ten years)...
March 18, 2019: Lipids in Health and Disease
Chunzhi Huang, Xu Hu, Zhaosheng Hou, Jianbo Ji, Zhonghao Li, Yuxia Luan
Graphene oxide (GO), as a drug delivery carrier, has attracted considerable attention because of its interesting properties. However, GO tends to aggregate in aqueous solution. Amphiphilic molecules are usually necessary to stabilize GO. The introduction of these non-functional macromolecules on the one hand reduces drug loading, but on the other hand may cause unpredictable side effects. This study proposes a new strategy for stabilizing GO with a functional photothermal agent, IR820 (new indocyanine green) derivative...
March 9, 2019: Journal of Colloid and Interface Science
Pius L Mwambene, Martina Kyallo, Eunice Machuka, Dedan Githae, Roger Pelle
Unraveling the genetic diversity of livestock species is central to understanding their value and importance for conservation and improvement in diverse production environments. In developing countries, information on genetic attributes of many livestock species is unfortunately scanty to support well-informed decision-making upon relevant management strategies. This study aimed at investigating allelic variability, genetic diversity, and genetic relationships of 10 indigenous chicken ecotypes from Southern Highlands of Tanzania using the Major Histocompatibility Complex-linked LEI0258 marker...
March 16, 2019: Poultry Science
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