Grégory Guirimand, Anthony Guihur, Catalina Perello, Michael Phillips, Samira Mahroug, Audrey Oudin, Thomas Dugé de Bernonville, Sébastien Besseau, Arnaud Lanoue, Nathalie Giglioli-Guivarc'h, Nicolas Papon, Benoit St-Pierre, Manuel Rodríguez-Concepcíon, Vincent Burlat, Vincent Courdavault
The Madagascar periwinkle ( Catharanthus roseus ) synthesizes the highly valuable monoterpene indole alkaloids (MIAs) through a long metabolic route initiated by the 2 C -methyl-D-erythritol 4-phosphate (MEP) pathway. In leaves, a complex compartmentation of the MIA biosynthetic pathway occurs at both the cellular and subcellular levels, notably for some gene products of the MEP pathway. To get a complete overview of the pathway organization, we cloned four genes encoding missing enzymes involved in the MEP pathway before conducting a systematic analysis of transcript distribution and protein subcellular localization...
April 7, 2020: Plants (Basel, Switzerland)