Wei Zhao, Yi Tang, Yang Yang, Min Wang, Haiyang Yu
Certain mechanical stimuli-particularly low-magnitude, high-frequency vibration-could induce bone marrow stem cell osteogenic differentiation and promote bone formation via Wnt signaling pathway, although the molecular mechanism is still unclear. In this study, we found that miR-335-5p is significantly upregulated after low-magnitude, high-frequency vibration, which suppresses the expression of the Wnt signaling inhibitor Dickkopf-related protein 1. Inhibition of miR-335-5p greatly reduced the osteogenic differentiation...
2019: Journal of Environmental Pathology, Toxicology and Oncology
Virginie Bottero, Judith A Potashkin
BACKGROUND: Dementia is a major public health concern affecting approximately 47 million people worldwide. Mild cognitive impairment (MCI) is one form of dementia that affects an individual's memory with or without affecting their daily life. Alzheimer's disease dementia (ADD) is a more severe form of dementia that usually affects elderly individuals. It remains unclear whether MCI is a distinct disorder from or an early stage of ADD. METHODS: Gene expression data from blood were analyzed to identify potential biomarkers that may be useful for distinguishing between these two forms of dementia...
October 30, 2019: International Journal of Molecular Sciences
Patryk Zarecki, Matthias Hackl, Johannes Grillari, Miguel Debono, Richard Eastell
CONTEXT: Vertebral fractures are the hallmark of osteoporosis. MicroRNAs (miRNAs) are a prominent class of gene regulators likely to affect bone homeostasis, including bone remodelling and fracture healing by altering gene expression in bone cells. OBJECTIVE: This study sought to compare the levels of circulating miRNAs in older women with osteoporotic vertebral fractures, and/or low BMD and healthy controls, and to correlate miRNAs expression levels with BTMs. DESIGN: A single-site, case-control, observational, cross-sectional study at a university hospital...
October 24, 2019: Bone
Wenwen Du, Haicheng Tang, Zhe Lei, Jianjie Zhu, Yuanyuan Zeng, Zeyi Liu, Jian-An Huang
BACKGROUND: Significant evidence has shown that the miRNA pathway is an important component in the downstream signaling cascades of TGF-β1 pathway. Our previous study has indicated that miR-335-5p expression was significantly down-regulated and acted as a vital player in the metastasis of non-small cell lung cancer (NSCLC), however the underlying mechanism remained unclear. METHODS: The differential expression level of miR-335-5p and ROCK1 were determined by qRT-PCR and IHC analysis in human tissue samples with or without lymph node metastasis...
October 21, 2019: Respiratory Research
Jennie Ong, Anke van den Berg, Alen Faiz, Ilse M Boudewijn, Wim Timens, Cornelis J Vermeulen, Brian G Oliver, Klaas Kok, Martijn M Terpstra, Maarten van den Berge, Corry-Anke Brandsma, Joost Kluiver
Cigarette smoking causes lung inflammation and tissue damage. Lung fibroblasts play a major role in tissue repair. Previous studies have reported smoking-associated changes in fibroblast responses and methylation patterns. Our aim was to identify the effect of current smoking on miRNA expression in primary lung fibroblasts. Small RNA sequencing was performed on lung fibroblasts from nine current and six ex-smokers with normal lung function. MiR-335-5p and miR-335-3p were significantly downregulated in lung fibroblasts from current compared to ex-smokers (false discovery rate (FDR) <0...
October 18, 2019: International Journal of Molecular Sciences
María Marcuello, Saray Duran-Sanchon, Lorena Moreno, Juan José Lozano, Luis Bujanda, Antoni Castells, Meritxell Gironella
Early detection of colorectal cancer (CRC) and its precancerous lesion, advanced adenomas (AA), is critical to improve CRC incidence and prognosis. Circulating microRNAs (miRNAs or miR) are promising non-invasive biomarkers for cancer detection. Our previous results showed that a plasma 6-miRNA signature (miR-15b-5p, miR-18a-5p, miR-29a-3p, miR-335-5p, miR-19a-3p and miR-19b-3p) could distinguish between CRC or AA and healthy individuals (controls). However, its diagnostic performance in serum is unknown. In this exploratory study we aim to evaluate the diagnostic performance of the 6-miRNA signature in serum samples in a cohort of individuals participating in Barcelona's CRC Screening Programme...
October 12, 2019: Cancers
Junxiang Lian, Xingwen Wu, Yao Liu, Wei Qiu, Xiaofang Zhu, Xiaoxuan Wang, Shu Meng, Paloma Valverde, Bjorn Steffensen, Qisheng Tu, Jian Pan, Jake Chen
BACKGROUND AND OBJECTIVE: Periodontitis is a prevalent oral disease responsible for tooth loss. MicroRNAs have been proven crucial in bone disorders over the past decades. Promotive effect on osteogenic activities by microRNA-335-5p (miR-335-5p) has been well demonstrated, but its role involved in the pathogenesis of periodontitis remains elusive. In this study, we established experimental periodontitis (EP) on transgenic mice overexpressing miR-335-5p (335-Tg) to investigate the novel effects of miR-335-5p on periodontal inflammation and bone loss...
September 21, 2019: Journal of Periodontal Research
Christian Lee Andersen, Rong Li, Xiaoqin Ye
Mycoestrogen zearalenone (ZEA) is a common food contaminant (ppb ~ low ppm levels) that can interfere with female reproduction. Previously we demonstrated disrupted mouse placental development by 40 ppm ZEA diet. MicroRNAs are sensitive to xenobiotics and have been implicated in placental physiology and pathology. We hypothesized that ZEA could dysregulate microRNA expression in the mouse placenta. Young mice were fed with 0 ppm (control), 4 ppm, and 40 ppm ZEA diets from 5.5 days post-coitum (D5.5). On D13...
September 2, 2019: Biology of Reproduction
Hamidreza Ebrahimiyan, Farhad Gharibdoost, Saeed Aslani, Hoda Kavosi, Faraneh Farsad, Ahmadreza Jamshidi, Mahdi Mahmoudi
Background : Survivin is an important anti-apoptotic protein and is involved in increasing auto-reactivity during the autoimmune diseases like Systemic sclerosis (SSc). Aims : In the current study, we investigate the expression level of total survivin (survivin-TS) and its three important variants alongside with evaluation of the expression level of important microRNAs (miRNAs) that are involved in survivin expression regulation. Methods : Peripheral blood mononuclear cells (PBMCs) were isolated from 50 healthy controls, 25 diffuse cutaneous SSc (DcSSc), and 25 limited cutaneous SSc (LcSSc) patients...
August 23, 2019: Modern Rheumatology
Jun Zou, Kun Wu, Chao Lin, Zhi-Gang Jie
Gastric cancer (GC) is one of the most common cancers in the world and remains a heavy burden of health worldwide. Adenylate cyclase 3 ( ADCY3 ) is a widely expressed membrane-associated protein in human tissues and has been identified to be a new molecular target of GC. Long non-coding RNAs (lncRNAs) have substantial influence on tumorigenesis and progression of tumors by binding to microRNAs (miRNAs). Therefore, this study is to clarify the mechanism by which LINC00319 sponges miR-335-5p to influence development of GC...
August 21, 2019: American Journal of Physiology. Gastrointestinal and Liver Physiology
Donglei Zhang, Ning Yang
PURPOSE: Colorectal cancer (CRC) is a common malignancy which has a high mortality rate around the world. The advancement of new therapeutic strategies is crucial for the efficient treatment of CRC. Many miRNAs play a central role in the progression of cancer cells. There are still few studies on miR-335-5p and CRC. In this study, the potential of miR-335-5p in CRC development and progression was explored. METHODS: The expression level of miR-335-5p in CRC cell lines and tissues was detected by quantitative real-time polymerase chain reaction (qRT-PCR) assay...
May 2019: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Lei Wang, Yajuan Zhao, Minyi Xu, Fangfang Zhou, Ji Yan
PURPOSE: B7-H3, a member of the B7 family of immune regulatory ligands, plays a critical role in the T cell-mediated immune response. It is broadly expressed in several human cancers and leads to poor prognosis. Nevertheless, the clinical significance of B7-H3 expression in colorectal cancer (CRC) remains unclear. METHODS: The serum B7-H3, B7-H1, cancer-associated carbohydrate antigen-50 (CA-50) and carcinoembryonic antigen (CEA) expressions in patients with CRC, benign gastrointestinal diseases, and healthy controls were measured by ELISA...
May 2019: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Maryam Kay, Bahram Mohammad Soltani, Fahimeh Hosseini Aghdaei, Hassan Ansari, Hossein Baharvand
BACKGROUND: WNT and TGFβ signaling pathways play critical regulatory roles in cardiomyocyte fate determination and differentiation. MiRNAs are also known to regulate different biological processes and signaling pathways. Here, we intended to find candidate miRNAs that are involved in cardiac differentiation through regulation of WNT and TGFβ signaling pathways. METHODS: Bioinformatics analysis suggested hsa-miR-335-3p and hsa-miR-335-5p as regulators of cardiac differentiation...
June 27, 2019: Stem Cell Research & Therapy
Haicheng Tang, Wenwen Du, Yongqian Jiang, Hongmiao Li, Hongjian Bo, Shu Song
Lung cancer is regarded as one of the dominant causes in cancer patients among men and women all over the world. Rho-associated coiled-coil forming protein kinase l (ROCK1) is characterized as pivotal downstream effectors of the small GTPase RhoA and reported to participate in tumor metastasis. miR-335-5p acts as tumor suppressor microRNA and is identified to be downregulated in tumor tissues. miR-335-5p/ROCK1 axis has been demonstrated to promote cell proliferation and metastasis in gastric cancer, hepatocellular carcinoma and so on...
May 29, 2019: Journal of Cellular Physiology
F H Yerlikaya, U Can, M S Alpaydin, A Aribas
OBJECTIVES: The aim of the study was to investigate the relationship between plasma microRNA expression levels, which are associated with lipid metabolism and serum trace element levels in patients with primary hyperlipidemia. METHODS: This study was performed on 46 (21M / 25F) primary hyperlipidemia patients aged 25-65 years and 37 (18 M/19 F) healthy people aged 25-65 years. RESULTS: The following miRNAs were upregulated: miR-33a-5p, miR-370-5p, miR-378a-3p, miR-27a-3p, miR-27a-5p and miR-335-5p...
2019: Bratislavské Lekárske Listy
Ketan S Patil, Indranil Basak, Ingvild Dalen, Esthelle Hoedt, Johannes Lange, Kristin A Lunde, Ying Liu, Ole-Bjørn Tysnes, Lars Forsgren, Dag Aarsland, Thomas A Neubert, Jan Petter Larsen, Guido Alves, Simon Geir Møller
INTRODUCTION: As current clinical diagnostic protocols for Parkinson's disease (PD) may be prone to inaccuracies there is a need to identify and validate molecular biomarkers, such as circulating microRNAs, which will complement current practices and increase diagnostic accuracy. This study identifies, verifies and validates combinatory serum microRNA signatures as diagnostic classifiers of PD across different patient cohorts. METHODS: 370 PD (drug naïve) and control serum samples from the Norwegian ParkWest study were used for identification and verification of differential microRNA levels in PD which were validated in a blind study using 64 NY Parkinsonism in UMeå (NYPUM) study serum samples and tested for specificity in 48 Dementia Study of Western Norway (DemWest) study Alzheimer's disease (AD) serum samples using miRNA-microarrays, and quantitative (q) RT-PCR...
April 11, 2019: Parkinsonism & related Disorders
Henri Charrier, Marie Cuvelliez, Emilie Dubois-Deruy, Paul Mulder, Vincent Richard, Christophe Bauters, Florence Pinet
Heart failure (HF) has several etiologies including myocardial infarction (MI) and left ventricular remodeling (LVR), but its progression remains difficult to predict in clinical practice. Systems biology analyses of LVR after MI provide molecular insights into this event such as modulation of microRNA (miRNA) that could be used as a signature of HF progression. To define a miRNA signature of LVR after MI, we use 2 systems biology approaches, integrating either proteomic data generated from LV of post-MI rat induced by left coronary artery ligation or multi-omics data (proteins and non-coding RNAs) generated from plasma of post-MI patients from the REVE-2 study...
March 7, 2019: Non-Coding RNA
Yu Yang, Feng Wang, Hang Huang, Yan Zhang, Hui Xie, Tongyi Men
Background: Bladder cancer (BC) is among the most frequently occurring cancer types in the urinary system. In recent years, the importance of lncRNAs in BC has been acknowledged. SLCO4A1-AS1 is an oncogene in colorectal cancer. However, the role of SLCO4A1-AS1 in BC remains unknown. Materials and methods: The expression levels of SLCO4A1-AS1 in BC tissues were analyzed by qRT-PCR. The effects of SLCO4A1-AS1 knockdown on proliferation were determined by CCK8 assay...
2019: OncoTargets and Therapy
Sophie Dhorne-Pollet, Elisa Crisci, Nuria Mach, Patricia Renson, Florence Jaffrézic, Guillemette Marot, Tatiana Maroilley, Marco Moroldo, Jérôme Lecardonnel, Fany Blanc, Nicolas Bertho, Olivier Bourry, Elisabetta Giuffra
Host miRNAs are known to modulate the cell response to virus infections. We characterized the miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain of PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). We performed the immunoprecipitation of RISC (RNA-induced Silencing Complex) followed by microarray analysis of the RISC-bound miRNA targets (RIP-Chip) to evaluate the relative enrichment or depletion of expressed genes in RISC. The miRNA-mediated regulation occurred early after PRRSV infection and decreased fast (1,241 and 141 RISC-bound genes at 7 h and 10 h post-infection, respectively); it affected several cell functions with evidence of miRNA buffering of upregulated interferon-related genes...
February 28, 2019: Scientific Reports
Yixin Liu, Peng Lai, Juelin Deng, Qiukui Hao, Xinyi Li, Ming Yang, Hui Wang, Birong Dong
AIM: MiR-335-5p expression might induce endothelial cells (ECs) aging and target inhibit sKlotho. This study aimed to investigate whether oxidative stress evoked miR-335-5p expression and whether miR-335-5p-regulated ECs function through sKlotho. METHODS: The expression of miR-335-5p was detected in human umbilical vein endothelial cells treated with H2 O2 . Subsequently, endothelial function and sKlotho expression were measured in human umbilical vein endothelial cells treated with H2 O2 and transfected with miR-335-5p mimics or inhibitor sequences...
February 20, 2019: Biomarkers in Medicine
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