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IL-8 melanocyte

Anna Niezgoda, Andrzej Winnicki, Tomasz Kosmalski, Bogna Kowaliszyn, Jerzy Krysiński, Rafał Czajkowski
BACKGROUND: Vitiligo is a chronic skin disorder presenting with depigmentation, the pathogenesis of which is complex but the autoimmune theory is now preferred. Multiple immunologic processes, including stimulation of the T-helper (Th)1 response, formation of autoreactive melanocyte-specific CD8+ T lymphocytes, a decrease in the blood concentration of T regulatory (Treg) cells, and an increase in interleukin (IL)-17 and interferon (IFN) concentration, have been shown to contribute to vitiligo progression and maintenance...
January 25, 2019: Trials
Genji Imokawa
In the first review, we summarized the biological effects of the xanthophyll carotenoid astaxanthin (AX) to prevent UV-induced cutaneous inflammation, abnormal keratinization, pigmentation and wrinkling in a manner independent of the depletion of reactive oxygen species. In this manuscript, we review what is known about the intracellular signaling mechanisms that are involved in those effects in keratinocytes and in melanocytes. Our research has characterized the intracellular stress signaling mechanism(s) that are involved in the up-regulated expression of genes encoding cyclooxygenase (COX2), interleukin (IL)-8, granulocyte macrophage colony stimulatory factor (GM-CSF) and transglutaminase (TGase)1 in UVB-exposed keratinocytes as well as in the stimulated transcription and/or translation of melanogenic factors, including microphthalmia-associated transcription factor (MITF), in stem cell factor (SCF)-treated melanocytes...
October 13, 2018: Photochemistry and Photobiology
Adrian V Cioanca, Peter J McCluskey, Steven S Eamegdool, Michele C Madigan
Toll-like receptors (TLRs) are a class of pattern recognition receptors that sense highly conserved pathogen associated antigenic determinants, triggering an innate immune response and subsequently instructing the adaptive immune system so that together, the pathogen can be eliminated. TLRs are widely distributed in human ocular tissues and cell types, and are active players in ocular inflammation. To date, the presence and function of TLRs on human choroidal melanocytes (HCMs), the most abundant choroidal cell type, have not been characterized...
August 2018: Experimental Eye Research
Sneha Mitra, Sritama De Sarkar, Ayan Pradhan, Ayan K Pati, Richeek Pradhan, Debolina Mondal, Sumit Sen, Arghyaprasun Ghosh, Suparna Chatterjee, Mitali Chatterjee
Vitiligo is an autoimmune depigmenting skin disease characterised by loss of melanocytes wherein oxidative stress is proposed to be the initial triggering factor with subsequent immune dysregulation. This study aimed to evaluate the relationship, if any, between the generation of reactive oxygen species (ROS), markers of oxidative damage and circulating cytokines in patients with active vitiligo. The generation of ROS in erythrocytes and neutrophils was significantly higher in patients with active vitiligo than healthy controls...
December 2017: Free Radical Research
Prashiela Manga, Nada Elbuluk, Seth J Orlow
Vitiligo, an acquired depigmentation disorder, manifests as white macules on the skin and can cause significant psychological stress and stigmatization. Recent advances have shed light on key components that drive disease onset and progression as well as therapeutic approaches. Vitiligo can be triggered by stress to the melanin pigment-producing cells of the skin, the melanocytes. The triggers, which range from sunburn to mechanical trauma and chemical exposures, ultimately cause an autoimmune response that targets melanocytes, driving progressive skin depigmentation...
2016: F1000Research
Markus Böhm, Mara Apel, Torsten Lowin, Julia Lorenz, Zsuzsa Jenei-Lanzl, Silvia Capellino, Heba Dosoki, Thomas A Luger, Rainer H Straub, Susanne Grässel
INTRODUCTION: The synovium is a target for neuropeptides. Melanocortins have attained particular attention as they elicit antiinflammatory effects. Although synovial fluid from patients with rheumatic diseases contains α-melanocyte-stimulating hormone (α-MSH) it is unknown whether synovial fibroblasts generate α-MSH and respond to melanocortins. METHODS: Synovial tissue was obtained from osteoarthritis (OA) patients. Cells were isolated and prepared either as primary mixed synoviocytes or propagated as synovial fibroblasts (OASFs)...
September 15, 2016: Biochemical Pharmacology
Jong Il Park, Ha Yeon Lee, Ji Eun Lee, Cheol Hwan Myung, Jae Sung Hwang
Melanosomes are lysosome-related organelles with specialized capabilities of melanin synthesis and movement mediated by the Rab27a-Melanophilin-MyosinVa protein complex. In this study, we found that 2-methyl-naphtho[1,2,3-de]quinolin-8-one (MNQO) induced melanosome aggregation around the nucleus in melan-a melanocytes and in melan-a melanocytes/SP-1 keratinocyte co-cultures without inducing toxicity or changing the melanin content. Western blot and real-time PCR analyses showed that MNQO decreased expression of the Rab27a, Melanophilin and MyosinVa proteins and mRNAs, respectively, in melan-a melanocytes...
July 6, 2016: Scientific Reports
Alessandra Tuccitto, Marcella Tazzari, Valeria Beretta, Francesca Rini, Claudia Miranda, Angela Greco, Mario Santinami, Roberto Patuzzo, Barbara Vergani, Antonello Villa, Giacomo Manenti, Loredana Cleris, Daniele Giardiello, Malcolm Alison, Licia Rivoltini, Chiara Castelli, Michela Perego
Melanoma is a highly heterogeneous tumor for which recent evidence supports a model of dynamic stemness. Melanoma cells might temporally acquire tumor-initiating properties or switch from a status of tumor-initiating cells (TICs) to a more differentiated one depending on the tumor context. However, factors driving these functional changes are still unknown. We focused on the role of cyto/chemokines in shaping TICs isolated directly from tumor specimens of two melanoma patients, namely Me14346S and Me15888S...
October 2016: Stem Cells
Ana Belén Gómez-SanMiguel, María Ángeles Villanúa, Ana Isabel Martín, Asunción López-Calderón
Sepsis induces anorexia and muscle wasting secondary to an increase in muscle proteolysis. Melanocyte stimulating hormones (MSH) is a family of peptides that have potent anti-inflammatory effects. Melanocortin receptor-3 (MC3-R) has been reported as the predominant anti-inflammatory receptor for melanocortins. The aim of this work was to analyse whether activation of MC3-R, by administration of its agonist D-Trp(8)-γMSH, is able to modify the response of skeletal muscle to inflammation induced by lipopolysaccharide endotoxin (LPS) or TNFα...
2016: PloS One
Lijun Sun, Shengjun Pan, Yuejin Yang, Jingying Sun, Daoyan Liang, Xin Wang, Xin Xie, Jun Hu
Toll-like receptors play essential roles in the modulation of melanogenesis, which has been implicated in the pathogenesis of hyper- or hypopigmentation-related diseases. However, little is currently known regarding the role of TLR9 in human melanocytes. TLR9 recognizes unmethylated cytosine-phosphate-guanine motif-containing oligodeoxynucleotides, and cytosine-phosphate-guanine ODN2006 acts as an hTLR9 agonist. The aim of the present study was to investigate the effect of cytosine-phosphate-guanine ODN2006 on melanogenesis in the human melanocyte cells...
August 2016: Experimental Biology and Medicine
Ana Belen Gómez-SanMiguel, Ana Isabel Martín, María Paz Nieto-Bona, Carmen Fernández-Galaz, María Ángeles Villanúa, Asunción López-Calderón
BACKGROUND: Chronic inflammatory diseases induce cachexia that increases mortality and morbidity of the illness. Adjuvant-induced arthritis is an experimental model of rheumatoid arthritis that is associated with body weight loss and muscle wasting. Alpha-melanocyte stimulating hormone has an anti-inflammatory effect in arthritic rats and decreases muscle wasting. The aim of this work was to elucidate whether the anti-cachectic action of alpha-melanocyte stimulating hormone is mediated by the melanocortin receptor type 3 pathway...
March 2016: Journal of Cachexia, Sarcopenia and Muscle
Shih-Chun Chao, Tommaso Vagaggini, Chan-Wei Nien, Sheng-Chieh Huang, Hung-Yu Lin
The effects of lutein and zeaxanthin on lipopolysaccharide- (LPS-) induced secretion of IL-8 by uveal melanocytes (UM) were tested in cultured human UM. MTT assay revealed that LPS (0.01-1 μg/mL) and lutein and zeaxanthin (1-10 μM) did not influence the cell viability of cultured UM. LPS caused a dose-dependent increase of secretion of IL-8 by cultured UM. Lutein and zeaxanthin did not affect the constitutive secretion of IL-8. However, lutein and zeaxanthin decreased LPS-induced secretion of IL-8 in cultured UM in a dose-dependent manner...
2015: Journal of Ophthalmology
Adriana Rodríguez-Arámbula, Bertha Torres-Álvarez, Diego Cortés-García, Cornelia Fuentes-Ahumada, Juan Pablo Castanedo-Cázares
The pathogenesis of melasma, a common, photo-induced hyperpigmentary disorder, is not clearly understood. Significant factors linked to melasma are ultraviolet radiation exposure and genetic predisposition. Histological analysis has demonstrated that melasma is caused by a network of cellular interactions among melanocytes, keratinocytes, mast cells, fibroblasts, and dermal vasculature exhibits, features similar to chronic sun damage. Dermal inflammation caused by ultraviolet radiation might play an important role in the hyperpigmentation and reactivation of melasma lesions through the production of melanogenic cytokines and growth factors...
October 2015: American Journal of Dermatopathology
Sunhyo Ryu, Andrew Johnson, Yoonkyung Park, Beomjoon Kim, David Norris, Cheryl A Armstrong, Peter I Song
Alpha-melanocyte stimulating hormone (α-MSH) is a highly conserved 13-aa neuropeptide derived from pro-opiomelanocortin by post-translational processing, which has been reported to exhibit potent anti-inflammatory activity and a wide range of immunosuppressive activities in the skin. However, the regulatory effect of α-MSH is not completely clear in cutaneous innate immunity. In this study, we investigate the functional regulation of α-MSH in TLR2-mediated inflammatory responses in normal human keratinocytes (HKs)...
2015: PloS One
Young Il Kim, Seung-Won Park, In Jung Kang, Min Kyung Shin, Mu-Hyoung Lee
Activins are dimeric growth and differentiation factors that belong to the transforming growth factor (TGF)-β superfamily of structurally related signaling proteins. In the present study, we examined the mechanisms through which activin regulates the lipopolysaccharide (LPS)-induced transcription of Toll-like receptors (TLRs), cytokines and inducible nitric oxide synthase (iNOS) in human melanocytes, as well as the involvement of nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signaling...
October 2015: International Journal of Molecular Medicine
Franco Capsoni, Anna Maria Ongari, Caterina Lonati, Riccardo Accetta, Stefano Gatti, Anna Catania
BACKGROUND: Alpha-melanocyte-stimulating-hormone (α-MSH) has marked anti-inflammatory potential. Proinflammatory cytokines are critical mediators of the disturbed cartilage homeostasis in osteoarthritis, inhibiting anabolic activities and increasing catabolic activities in chondrocytes. Since human chondrocytes express α-MSH receptors, we evaluated the role of the peptide in modulating chondrocyte production of pro-inflammatory cytokines, matrix metalloproteinases (MMPs), tissue inhibitors of MMPs (TIMPs), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in response to interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)...
June 21, 2015: BMC Musculoskeletal Disorders
Jin Hee Kim, Se Mi Lee, Cheol Hwan Myung, Kyung Rhim Lee, Seung Min Hyun, Ji Eun Lee, Young Sun Park, Se Rim Jeon, Jong Il Park, Sung Eun Chang, Jae Sung Hwang
β-Lapachone is an ortho naphthoquinone obtained from the bark of the lapacho tree (Tabebuia avellanedae), which has been used medicinally for centuries. The purpose of this study was to investigate the effects of β-lapachone on inhibitory mechanism of melanogenesis. β-Lapachone inhibited melanin synthesis and tyrosinase activity at 0.8 μM in melan-a cells. Also, β-lapachone reduced the expression of tyrosinase and tyrosinase-related protein-1 at transcriptional and translational levels. The decreased expression of tyrosinase and tyrosinase-related protein-1 might result from the reduced microphthalmia-associated transcription factor (MITF) level which regulates major melanogenic proteins...
April 2015: Archives of Dermatological Research
Ondřej Kodet, Lukáš Lacina, Eliška Krejčí, Barbora Dvořánková, Miloš Grim, Jiří Štork, Daniela Kodetová, Čestmír Vlček, Jana Šáchová, Michal Kolář, Hynek Strnad, Karel Smetana
BACKGROUND: Nodular melanoma is one of the most life threatening tumors with still poor therapeutic outcome. Similarly to other tumors, permissive microenvironment is essential for melanoma progression. Features of this microenvironment are arising from molecular crosstalk between the melanoma cells (MC) and the surrounding cell populations in the context of skin tissue. Here, we study the effect of melanoma cells on human primary keratinocytes (HPK). Presence of MC is as an important modulator of the tumor microenvironment and we compare it to the effect of nonmalignant lowly differentiated cells also originating from neural crest (NCSC)...
January 5, 2015: Molecular Cancer
M Nadim, D Auriol, N Lamerant-FayeL, F Lefèvre, L Dubanet, G Redziniak, C Kieda, C Grillon
OBJECTIVE: Polyphenols are strong antioxidant molecules allowing prevention of skin photo-ageing damages, but their use is limited due to low solubility and toxicity towards skin cells. We postulated that enzymatic glucosylation could improve their solubility, stability and, consequently, their efficacy. The aim of this work was to study changes induced by addition of a glucose moiety on two polyphenols displaying very different chemical structures [caffeic acid (CA), epigallocatechin-3-gallate (EGCG) and there glucosylated form, Glc-CA and Glc-EGCG] by assessing their cytotoxic properties and their antioxidant and anti-inflammatory activities...
December 2014: International Journal of Cosmetic Science
Dan-Ning Hu, Mingchao Bi, David Y Zhang, Fei Ye, Steven A McCormick, Chi-Chao Chan
PURPOSE: Melanocytes are one of the major cellular components in the uvea. Interleukin-8/CXCL8 and monocyte chemoattractant protein-1 (MCP-1/CCL2) are the two most important proinflammatory chemokines. We studied the constitutive and lipopolysaccharide (LPS)-induced expression of IL-8 and MCP-1 in cultured human uveal melanocytes (UM) and explored the relevant signal pathways. METHODS: Conditioned media and cells were collected from UM cultured in medium with and without stimulation of LPS...
September 2014: Investigative Ophthalmology & Visual Science
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