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F J Rodríguez-Lozano, M Collado-González, C J Tomás-Catalá, D García-Bernal, S López, R E Oñate-Sánchez, J M Moraleda, L Murcia
OBJECTIVES: To evaluate in vitro the cementogenic potential and the biological effects of GuttaFlow Bioseal, GuttaFlow 2, MTA Fillapex and AH Plus on human periodontal ligament stem cells (hPDLSCs). METHODS: Cell viability, cell migration and cell morphology assays were performed using eluates of each material. To evaluate cell attachment, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The effects of endodontic sealers on cementum protein 1 (CEMP1), cementum-derived attachment protein (CAP), bone sialoprotein (BSP), ameloblastin (AMBN), amelogenin (AMELX) and alkaline phosphatase (ALP) gene expression on hPDLSCs were investigated by qPCR and immunofluorescence (IF)...
November 19, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Niko-Pekka Johannes Hoikkala, Xiaoju Wang, Leena Hupa, Jan-Henrik Smått, Jouko Peltonen, Pekka K Vallittu
Aim of the study was to evaluate apatite forming ability and ion dissolution of bioactive glass-ceramic (BGC) particles from novel polydimethylsiloxane (PDMS) based endodontic sealer Guttaflow Bioseal. Firstly, water sorption and solubility were determined for Guttaflow 2 and Guttaflow Bioseal (GB), the latter containing BGC filler particles. Mineral precipitations in simulated body fluid (SBF) were observed with SEM/EDX. Ion-release from the sealer was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) in Tris-buffer solution...
August 29, 2018: Dental Materials Journal
Małgorzata Pawińska, Elżbieta Łuczaj-Cepowicz, Grzegorz Szczurko, Anna Kierklo, Grażyna Marczuk-Kolada, Katarzyna Leszczyńska
BACKGROUND: Actinomyces species have a low virulence and pathogenicity, but under specific circumstances they may be involved in root canal and periapical tissue infections. OBJECTIVES: The aim of the study was to investigate the antibacterial activity of various root canal sealers on standardized strains of Actinomyces. MATERIAL AND METHODS: The materials tested in this study included AH Plus™ Jet (AH), Apexit® Plus (AP), Endomethasone N (EN), GuttaFlow® (GF), Hybrid Root SEAL (HB), MTA Fillapex (FL), Real® Seal (RCS), Roeko Seal Automix (RSA), Sealapex™ (SP), and Tubli-Seal™ (TS)...
August 7, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Vassiliki Taraslia, Ema Anastasiadou, Christina Lignou, Georgios Keratiotis, Anastasia Agrafioti, Evangelos G Kontakiotis
Objective: The aim of this study was to evaluate the viability of human periodontal ligament (PDL) cells on MTA-Fillapex, GuttaFlow 2, TotalFill Sealer, and BioRoot™ RCS in comparison to conventional epoxy resin-based (AH Plus) and zinc oxide-eugenol-based (Roth's 801) sealers. Materials and Methods: Sealers were divided into two groups, and five coverslips for each material per group were prepared. In the first group, PDLs were added immediately after the preparation of sealers (Fresh Group), and in the second, PDLs were added after 24 h...
April 2018: European Journal of Dentistry
Haridas Das Adhikari, Sakshi Jain
Background: Not only the gaps at dentin-sealer interface but also at core-sealer interface may jeopardize the outcome of root canal treatment. Aim: The aim of this in vitro scanning electron microscopic (SEM) study was to determine which root canal sealer among AH-Plus, GuttaFlow, and RealSeal provides a superior marginal adaptation with the core obturating material in the apical third region of root canals. Materials and Methods: Selected 30 human freshly extracted maxillary central incisors were biomechanically prepared, then divided equally into three groups and obturated with AH-Plus, GuttaFlow, and RealSeal using single cone obturation technique...
January 2018: Journal of Conservative Dentistry: JCD
Sakshi Jain, Haridas Das Adhikari
Background: Complete seal at dentin-sealer-core interface of the root canal is required to maintain long-term periapical health. Aim: The aim of this in vitro scanning electron microscopic (SEM) study was to determine which of the commonly used root canal sealer among AH-Plus, GuttaFlow, and RealSeal provides a superior marginal adaptation with the dentin in the apical third region of root canals. Materials and Methods: Selected 30 human freshly extracted maxillary central incisors were biomechanically prepared, then divided equally into three groups and obturated with AH-Plus, GuttaFlow, and RealSeal using single-cone obturation technique...
January 2018: Journal of Conservative Dentistry: JCD
Grzegorz Szczurko, Małgorzata Pawińska, Elżbieta Łuczaj-Cepowicz, Anna Kierklo, Grażyna Marczuk-Kolada, Adam Hołownia
The aim of the study was to compare ex vivo the toxic effects of six root canal sealers immediately after mixing or setting on human periodontal ligament fibroblasts (HPdLF). Freshly mixed (I group) or set (allowed to dry for 24 h) (II group) specimens of AH Plus Jet (AH), Apexit Plus (AP), MTA Fillapex (FL), GuttaFlow (GF), MetaSEAL Soft (META), and Tubli-Seal (TS) were prepared. HPdLF were exposed for 24 h to the specimens. 3-(4,5-dimethylthiazolo-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the effect of the root canal sealers on mitochondrial metabolic activity...
July 2018: Odontology
Vasileios Kapralos, Andreas Koutroulis, Dag Ørstavik, Pia Titterud Sunde, Håkon Valen Rukke
INTRODUCTION: The aim of this study was to investigate the antibacterial activity of 4 endodontic sealers against bacteria planktonic grown or in biofilms commonly detected from persistent and secondary endodontic infections. METHODS: The antibacterial activity of the sealers AH Plus, TotalFill BC sealer, RoekoSeal, and Guttaflow 2 was investigated for planktonic grown and 24-hour-old biofilms of Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, and Streptococcus mutans...
January 2018: Journal of Endodontics
Mario Tanomaru-Filho, Fernanda Ferrari Esteves Torres, Gisselle Moraima Chávez-Andrade, Madelise de Almeida, Luciana Guilherme Navarro, Liviu Steier, Juliane Maria Guerreiro-Tanomaru
INTRODUCTION: This study evaluated setting time (ST), radiopacity, pH, flow, solubility, and volumetric change (VC) of a silicone, gutta-percha, and bioactive glass-based sealer, GuttaFlow Bioseal (GFB), and a calcium silicate-based sealer, TotalFill BC Sealer (TFBC), in comparison with AH Plus. METHODS: ST and flow were evaluated in accordance with the ISO 6876 Standard. pH was evaluated after different time intervals (1, 3, 7, 14, 21, and 28 days). Radiopacity was evaluated by radiographic analysis in millimeters of aluminum...
December 2017: Journal of Endodontics
Rafael Verardino de Camargo, Yara Terezinha Corrêa Silva-Sousa, Rodrigo Presotto Ferreira da Rosa, Jardel Francisco Mazzi-Chaves, Fabiane Carneiro Lopes, Liviu Steier, Manoel Damião Sousa-Neto
To assess the physicochemical properties of AH Plus, GuttaFlow 2, GuttaFlow BioSeal, and MM Seal, five samples of each root canal sealer were evaluated to determine their setting time (ST), dimensional change (DC), solubility (SL), flow (FL), and radiopacity (RD) according to American National Standards Institute/American Dental Association (ANSI/ADA) Specification 57. The distilled and deionized water obtained from the SL test were subjected to atomic absorption spectrometry to observe the presence of Ca2+, K+, and Na+ ions...
August 21, 2017: Brazilian Oral Research
Gokhan Saygili, Suna Saygili, Ibrahim Tuglu, Ismail Davut Capar
INTRODUCTION: The aim of the present in vitro study was to evaluate the cytotoxicity of different sealers including GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex on L929 murine fibroblasts. METHODS AND MATERIALS: Samples of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex were fabricated in Teflon disks of 5 mm diameter and 3 mm thickness. L929 fibroblasts were exposed to the extracts of these materials for 3, 24, 72 and 168 h at 37(°)C with 5% CO2...
2017: Iranian Endodontic Journal
Małgorzata Pawińska, Grzegorz Szczurko, Anna Kierklo, Jarosław Sidun
BACKGROUND: Alkaline pH is responsible for antibacterial activity and the stimulation of periapical tissue healing. It neutralizes the acidic environment of inflammatory tissues in the periapical region of the teeth and favors bone repair by activating tissue enzymes. OBJECTIVES: The aim of this study was to evaluate and compare in vitro the pH of 8 root canal filling materials (sealers and points) -AH Plus Jet (AH), Apexit Plus (AP), Endomethasone N (END), Epiphany (EP), GuttaFlow (GF), gutta-percha (G), Resilon (R), Tubliseal (T)...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Vibha Hegde, Laxmi Suresh Murkey
INTRODUCTION: The purpose of an endodontic obturation is to obtain a fluid tight hermetic seal of the entire root canal system. There has been an evolution of different materials and techniques to achieve this desired gap free fluid tight seal due to presence of anatomic complexity of the root canal system. AIM: To compare the microgap occurring in root canals obturated with hydrophilic versus hydrophobic systems using scanning electron microscope. MATERIALS AND METHODS: Sixty extracted human single-rooted premolars were decoronated, instrumented using NiTi rotary instruments...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
Chad W Lothamer, Ashley Anderson, Scott J Hetzel, Alex I Adrian, Christopher Snyder, Stephanie Goldschmidt, Jason W Soukup
An apical dye leakage test was utilized to compare 2 endodontic sealer systems commonly used in veterinary endodontic treatment. Rotary instrumentation followed by injection of 2 different sealers (GuttaFlow 2 and AH Plus) and a single master cone obturation technique were compared. Following obturation and restoration, specimens were immersed in India ink for 48 hours. A tooth mineral clearing technique was utilized to render the tooth samples transparent. Subsequently, the apices were evaluated for prevalence and magnitude of apical dye penetration under magnification...
June 2017: Journal of Veterinary Dentistry
Mar Collado-González, Christopher J Tomás-Catalá, Ricardo E Oñate-Sánchez, José M Moraleda, Francisco J Rodríguez-Lozano
INTRODUCTION: The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment. METHODS: Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer...
May 2017: Journal of Endodontics
Kursat Er, Ahmet Ayar, Omer-Faruk Kalkan, Sinan Canpolat, Tamer Tasdemir, Ulku Ozan
BACKGROUND: The aim of this study was to investigate the possible neurotoxic effects of 3 root canal sealers (RCSs) (AH Plus, GuttaFlow, iRoot SP) on cultured rat trigeminal ganglion (TG) neurons. MATERIAL AND METHODS: Primary cultures of TG neurons were obtained from 1 to 2-day old rats. Freshly mixed RCSs were incubated in sterile phosphate buffered saline and cells were incubated with supernatants of the RCSs for different time intervals (1-, 3-, 6- and 24-h; 1 or 1/10 diluted) and viability/cytotoxicity was tested by counting the number of live cells...
January 2017: Journal of Clinical and Experimental Dentistry
Flávia Emi Razera Baldasso, Patrícia Maria Poli Kopper, Renata Dornelles Morgental, Liviu Steier, José Antônio Poli de Figueiredo, Roberta Kochenborger Scarparo
Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another...
October 2016: Brazilian Dental Journal
Manuel Monterde, Antonio Pallarés, Cristina Cabanillas, Ignacio Zarzosa, Alejandro Victoria
AIM: A comparison was made between the root canal lateral condensation technique and other condensation techniques in which physical (temperature: Soft-Core®, Obtura II®) or chemical changes (condensation: Guttaflow®, Resilon®) may affect the dimensional stability of obturation and thus favor apical microleakage. MATERIALS AND METHODS: A total of 212 single-root teeth removed for orthodontic or periodontal reasons were randomized to 5 groups of 40 teeth each...
June 2014: Acta Stomatologica Croatica
Prajakta Patil, Vishnu P S Rathore, Chetan Hotkar, Snehal S Savgave, K Raghavendra, Priya Ingale
AIMS: The present study aimed to compare apical sealing ability between GuttaFlow and AH Plus. MATERIALS AND METHOD: Eighty extracted human maxillary anterior teeth with fully formed apex and straight root were collected for this study. The root canals were cleaned and shaped using a standard step back preparation to size 60# master apical file at the established working length and divided into four groups: Group 1, GuttaFlow sealer with gutta-percha; Group 2, AH Plus sealer with gutta-percha; Group 3, positive control group (Teeth were instrumented and left without obturation); Group 4, negative control group (Teeth were totally coated with nail varnish) Dye leakage was carried out...
July 2016: Journal of International Society of Preventive & Community Dentistry
Sedigheh Khedmat, Abbas Azari, Ahmad Reza Shamshiri, Mehdi Fadae, Hoorieh Bashizadeh Fakhar
INTRODUCTION: The aim of this in vitro study was to compare the efficacy of ProTaper retreatment (ProTaper R) and Mtwo retreatment (Mtwo R) files in removing gutta-percha and GuttaFlow from endodontically treated straight root canals. METHODS AND MATERIALS: The root canals of 60 human mandibular single-rooted premolars were prepared and randomly divided into two groups (n=30). In groups A and B the root canals were obturated using lateral condensation of gutta-percha plus AH 26 and GuttaFlow, respectively...
2016: Iranian Endodontic Journal
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