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F J Rodríguez-Lozano, M Collado-González, C J Tomás-Catalá, D García-Bernal, S López, R E Oñate-Sánchez, J M Moraleda, L Murcia
OBJECTIVES: To evaluate in vitro the cementogenic potential and the biological effects of GuttaFlow Bioseal, GuttaFlow 2, MTA Fillapex and AH Plus on human periodontal ligament stem cells (hPDLSCs). METHODS: Cell viability, cell migration and cell morphology assays were performed using eluates of each material. To evaluate cell attachment, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The effects of endodontic sealers on cementum protein 1 (CEMP1), cementum-derived attachment protein (CAP), bone sialoprotein (BSP), ameloblastin (AMBN), amelogenin (AMELX) and alkaline phosphatase (ALP) gene expression on hPDLSCs were investigated by qPCR and immunofluorescence (IF)...
November 19, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Niko-Pekka Johannes Hoikkala, Xiaoju Wang, Leena Hupa, Jan-Henrik Smått, Jouko Peltonen, Pekka K Vallittu
Aim of the study was to evaluate apatite forming ability and ion dissolution of bioactive glass-ceramic (BGC) particles from novel polydimethylsiloxane (PDMS) based endodontic sealer Guttaflow Bioseal. Firstly, water sorption and solubility were determined for Guttaflow 2 and Guttaflow Bioseal (GB), the latter containing BGC filler particles. Mineral precipitations in simulated body fluid (SBF) were observed with SEM/EDX. Ion-release from the sealer was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) in Tris-buffer solution...
August 29, 2018: Dental Materials Journal
Zahra Shourgashti, Hamid Keshvari, Hasan Torabzadeh, Mostafa Rostami, Shahin Bonakdar, Saeed Asgary
Introduction: The objective of this study was to evaluate the physical properties, cytotoxicity and sealing ability of HealApex _a new premixed calcium-silicate-phosphate-based biosealer_ in comparison with AH-26. Methods and Materials: Setting time, working time, film thickness, flow and radiopacity evaluation were performed according to ISO 6876 specification. L929 fibroblasts were incubated with the extracts of sealers and cytotoxicity was then evaluated using MTT assay...
2018: Iranian Endodontic Journal
Mario Tanomaru-Filho, Fernanda Ferrari Esteves Torres, Gisselle Moraima Chávez-Andrade, Madelise de Almeida, Luciana Guilherme Navarro, Liviu Steier, Juliane Maria Guerreiro-Tanomaru
INTRODUCTION: This study evaluated setting time (ST), radiopacity, pH, flow, solubility, and volumetric change (VC) of a silicone, gutta-percha, and bioactive glass-based sealer, GuttaFlow Bioseal (GFB), and a calcium silicate-based sealer, TotalFill BC Sealer (TFBC), in comparison with AH Plus. METHODS: ST and flow were evaluated in accordance with the ISO 6876 Standard. pH was evaluated after different time intervals (1, 3, 7, 14, 21, and 28 days). Radiopacity was evaluated by radiographic analysis in millimeters of aluminum...
December 2017: Journal of Endodontics
M Torabinejad, M Parirokh, P M H Dummer
Mineral trioxide aggregate (MTA) is a dental material used extensively for vital pulp therapies (VPT), protecting scaffolds during regenerative endodontic procedures, apical barriers in teeth with necrotic pulps and open apices, perforation repairs as well as root canal filling and root-end filling during surgical endodontics. A number of bioactive endodontic cements (BECs) have recently been introduced to the market. Most of these materials have calcium and silicate in their compositions; however, bioactivity is a common property of these cements...
March 2018: International Endodontic Journal
M Parirokh, M Torabinejad, P M H Dummer
Mineral trioxide aggregate (MTA) is a bioactive endodontic cement (BEC) mainly comprised of calcium and silicate elements. The cement was introduced by Torabinejad in the 1990s and has been approved by the Food and Drug Administration to be used in the United States in 1997. A number of new BECs have also been introduced to the market, including BioAggregate, Biodentine, BioRoot RCS, calcium-enriched mixture cement, Endo-CPM, Endocem, EndoSequence, EndoBinder, EndoSeal MTA, iRoot, MicroMega MTA, MTA Bio, MTA Fillapex, MTA Plus, NeoMTA Plus, OrthoMTA, Quick-Set, RetroMTA, Tech Biosealer and TheraCal LC...
February 2018: International Endodontic Journal
Rafael Verardino de Camargo, Yara Terezinha Corrêa Silva-Sousa, Rodrigo Presotto Ferreira da Rosa, Jardel Francisco Mazzi-Chaves, Fabiane Carneiro Lopes, Liviu Steier, Manoel Damião Sousa-Neto
To assess the physicochemical properties of AH Plus, GuttaFlow 2, GuttaFlow BioSeal, and MM Seal, five samples of each root canal sealer were evaluated to determine their setting time (ST), dimensional change (DC), solubility (SL), flow (FL), and radiopacity (RD) according to American National Standards Institute/American Dental Association (ANSI/ADA) Specification 57. The distilled and deionized water obtained from the SL test were subjected to atomic absorption spectrometry to observe the presence of Ca2+, K+, and Na+ ions...
August 21, 2017: Brazilian Oral Research
Gokhan Saygili, Suna Saygili, Ibrahim Tuglu, Ismail Davut Capar
INTRODUCTION: The aim of the present in vitro study was to evaluate the cytotoxicity of different sealers including GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex on L929 murine fibroblasts. METHODS AND MATERIALS: Samples of GuttaFlow Bioseal, GuttaFlow 2, AH-Plus and MTA Fillapex were fabricated in Teflon disks of 5 mm diameter and 3 mm thickness. L929 fibroblasts were exposed to the extracts of these materials for 3, 24, 72 and 168 h at 37(°)C with 5% CO2...
2017: Iranian Endodontic Journal
Mar Collado-González, Christopher J Tomás-Catalá, Ricardo E Oñate-Sánchez, José M Moraleda, Francisco J Rodríguez-Lozano
INTRODUCTION: The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment. METHODS: Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer...
May 2017: Journal of Endodontics
M G Gandolfi, F Siboni, C Prati
OBJECTIVE: Root canal filling sealers based on polymethyl hydrogensiloxane or polymethyl hydrogensiloxane-guttapercha--introduced to improve the quality of conventional guttapercha-based and resin-based systems--showed advantages in handiness and clinical application. The aim of the study was to evaluate the chemical-physical properties of a novel polysiloxane-guttapercha calcium silicate-containing root canal sealer (GuttaFlow bioseal). METHODS: GuttaFlow bioseal was examined and compared with GuttaFlow2, RoekoSeal and MTA Fillapex sealers...
May 2016: Dental Materials: Official Publication of the Academy of Dental Materials
Merve Akcay, Hakan Arslan, Nazli Durmus, Merve Mese, Ismail Davut Capar
BACKGROUND AND OBJECTIVE: Varied physical and chemical characteristic of root canal sealers and different irrigant agitation systems can influence the depth of penetration. The aim of this in vitro study was to use a laser scanning confocal microscope in order to assess the dentinal tubules penetration of various sealers after the application of different final irrigation techniques. STUDY DESIGN/MATERIALS AND METHODS: A total of 156 single-rooted extracted mandibular premolars were prepared up to size 40 and randomly distributed into four groups according to the sealer type (n = 39): AH Plus, iRoot SP, MTA Fillapex, and GF Bioseal...
January 2016: Lasers in Surgery and Medicine
Heba M El-Khodary, Deema J Farsi, Najat M Farsi, Ahmed Z Zidan
AIM: The aim of this study was to compare the sealing ability of mineral trioxide aggregate (MTA), Portland cement (PC), Biodentine(TM) and Tech biosealer in repairing furcal perforations in primary molars using the fluid-filtration technique. MATERIALS AND METHODS: Fifty freshly extracted maxillary second primary molars were sectioned horizontally at the furcation region to create dentin disks of 1.5 mm (+ 0.1 mm) thickness. Five disks were not perforated and served as negative controls...
September 1, 2015: Journal of Contemporary Dental Practice
Angela Delfina Bittencourt Garrido, Sueli Patricia Harumi Miyagi de Cara, Marcia Martins Marques, Emílio Carlos Sponchiado, Lucas da Fonseca Roberti Garcia, Manoel Damião de Sousa-Neto
BACKGROUND: The constant development of new root canal sealers has allowed the solution of a large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as filling materials. The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells. MATERIALS AND METHODS: The experimental groups were formed according to the culture medium conditioned with the tested sealers, as follows: Control group (CG) (culture medium without conditioning); Sealer 26 (S26) - culture medium + S26; Endofill (EF) - culture medium + EF; AH Plus (AHP) - culture medium + AHP; and BS - culture medium + BS (Copaiba oil-based sealer)...
March 2015: Dental Research Journal
Maria Giovanna Gandolfi, Francesco Siboni, Tatiana Botero, Maurizio Bossù, Francesco Riccitiello, Carlo Prati
AIM: The chemical-physical properties of novel and long-standing calcium silicate cements versus conventional pulp capping calcium hydroxide biomaterials were compared. METHODS: Calcium hydroxide-based (Calxyl, Dycal, Life, Lime-Lite) and calcium silicate-based (ProRoot MTA, MTA Angelus, MTA Plus, Biodentine, Tech Biosealer capping, TheraCal) biomaterials were examined. Calcium and hydroxyl ion release, water sorption, interconnected open pores, apparent porosity, solubility and apatite-forming ability in simulated body fluid were evaluated...
January 2015: Journal of Applied Biomaterials & Functional Materials
Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
OBJECTIVES: This study was performed to evaluate the cytotoxicity of four calcium silicate-based endodontic cements at different storage times after mixing. MATERIALS AND METHODS: Capillary tubes were filled with Biodentine (Septodont), Calcium Enriched Mixture (CEM cement, BioniqueDent), Tech Biosealer Endo (Tech Biosealer) and ProRoot MTA (Dentsply Tulsa Dental). Empty tubes and tubes containing Dycal were used as negative and positive control groups respectively...
August 2014: Restorative Dentistry & Endodontics
Boniek Castillo Borges, Adrielle Silva Barreto, Cláudia Lobelli Gomes, Talles Rodrigo Silva, Clodomiro Alves-Júnior, Isauremi Vieira Pinheiro, Rodivan Braz, Marcos Antonio Montes
This study aimed to evaluate whether preheated resin-based flowable restoratives would show increased hardness and softening susceptibility after an early cariogenic challenge. Fluroshield- Yellowed, Bioseal, Wave, Master Flow, Fluroshield-White, Conseal F, Filtek Z350 Flow, and Opallis Flow were tested. Preheating was performed using a microwave device. Five specimens of each preheated or room temperature material (n = 5) were fabricated. Hardness was assessed before and after a cariogenic challenge. The analysis was done by two-way analysis of variance (ANOVA) with repeated measures (cariogenic challenge) and Tukey's test for multiple comparisons (...
2013: European Journal of Esthetic Dentistry: Official Journal of the European Academy of Esthetic Dentistry
Hüseyin Sinan Topçuoğlu, Öznur Tuncay, Ertuğrul Karataş, Hakan Arslan, Kübra Yeter
INTRODUCTION: The aim of this study was to evaluate the fracture resistance of teeth filled with 3 different endodontic sealers. METHODS: Seventy-five single-rooted extracted mandibular premolars were decoronated to a length of 13 mm. The teeth were randomly divided into 5 groups (n = 15 for each group). In group 1, the teeth were left unprepared and unfilled (negative control), and in group 2, the teeth were left unobturated (positive control). The rest of the roots were prepared by using the ProTaper System up to a master apical file size of F3: group 3, bioceramic sealer (Endosequence BC sealer) + gutta-percha; group 4, mineral trioxide aggregate-based sealer (Tech Biosealer Endo) + gutta-percha; and group 5, epoxy resin-based sealer (AH Plus Jet) + gutta-percha...
December 2013: Journal of Endodontics
Navdeep Kaur Dhami, M Sudhakara Reddy, Abhijit Mukherjee
Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. In the present study, calcifying bacteria, Bacillus megaterium SS3 isolated from calcareous soil was applied as biosealant to enhance the durability of low energy, green building materials (soil-cement blocks). This bacterial isolate produced high amounts of urease, carbonic anhydrase, extra polymeric substances and biofilm. The calcium carbonate polymorphs produced by B...
December 2013: World Journal of Microbiology & Biotechnology
Maria Giovanna Gandolfi, Paola Taddei, Enrico Modena, Francesco Siboni, Carlo Prati
Commercial root end filling materials, namely two zinc oxide eugenol-based cements [intermediate restorative material (IRM), Superseal], a glass ionomer cement (Vitrebond) and three calcium-silicate mineral trioxide aggregate (MTA)-based cements (ProRoot MTA, MTA Angelus, and Tech Biosealer root end), were examined for their ability to: (a) release calcium (Ca(2+) ) and hydroxyl (OH(-) ) ions (biointeractivity) and (b) form apatite (Ap) and/or calcium phosphate (CaP) precursors. Materials were immersed in Hank's balanced salt solution (HBSS) for 1-28 days...
October 2013: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Xiuwen Wu, Jianan Ren, Yujie Yuan, Jianfeng Luan, Genhong Yao, Jieshou Li
Fibrin glue is a promising alternative for low-output enterocutaneous fistula closure. Bacterial flora colonizing inside the fistula tract, however, may limit the glue application. Single-donor-derived, platelet-rich materials were hypothesized in this study to have antimicrobial activity against Gram-negative microorganisms. Platelet-leukocyte fibrin (PLF), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were obtained from healthy volunteers. The amounts of platelet, leukocyte, and complement/antibody were determined...
2013: Platelets
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