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Fernando Pérez-Sanz, Beatriz Revilla-Nuin, Laura Marínez-Alarcón, José I Herrero, Pablo Ramírez, José A Pons, Alberto Baroja-Mazo
BACKGROUND: Numerous studies have emphasized the genetic and phenotypic profiles of tolerant transplant patients. Moreover, different groups have defined several biomarkers, trying to distinguish patients who are going to be tolerant from those who are going to reject. However, most of these biomarkers have not been validated by other groups or even established for clinical practice. METHODS: We reanalyzed and stratified the predictive capacity of 20 previously described biomarkers for liver transplantation tolerance in a cohort of 17 liver transplant patients subjected to an independent, nonrandomized, prospective study of immunosuppression drug withdrawal...
January 30, 2019: Transplantation
J-F Mu, X-D Wang, P-D Sun
OBJECTIVE: This study aimed to investigate the expression of miR-31 in rectal cancer patients and its effect on the proliferation and invasion ability of human rectal cancer cells SW837. PATIENTS AND METHODS: 55 rectal cancer cancerous tissue specimens and 55 corresponding adjacent tissue (tissue adjacent to carcinoma) specimens were collected from rectal cancer patients treated in The First Hospital of Jilin University from March 2014 to March 2015. Real Time-quantitative Polymerase Chain Reaction was used for detecting the expression level of miR-31 in cancerous tissue and corresponding adjacent tissues...
December 2018: European Review for Medical and Pharmacological Sciences
Anna Barbara Di Stefano, Daniela Massihnia, Federica Grisafi, Marta Castiglia, Francesca Toia, Luigi Montesano, Antonio Russo, Francesco Moschella, Adriana Cordova
Angiogenesis is a crucial process for the maintenance of normal tissue physiology and it is involved in tissue remodeling and regeneration. This process is essential for adipose tissue maintenance. The adipose tissue is composed by different cell types including stromal vascular cells as well as adipose stem cells (ASCs). In particular, ASCs are multipotent somatic stem cells that are able to differentiate and secrete several growth factors; they are recently emerging as a new cell reservoir for novel therapies and strategies in many diseases...
November 30, 2018: European Journal of Cell Biology
Ling Xu, Yu Fu, Weiwen Zhu, Rongyao Xu, Juan Zhang, Ping Zhang, Jie Cheng, Hongbing Jiang
BACKGROUND: Cleidocranial dysplasia (CCD) in humans is an autosomal-dominant skeletal dysplasia caused by heterozygous mutations of the runt-related transcription factor 2 (RUNX2) and significantly increases the risk of osteoporosis. Increasing evidence demonstrates that the dysfunction of bone marrow stromal cells from CCD patients (BMSCs-CCD) contributes to the bone deficiency, but the characteristics of BMSCs-CCD and the mechanisms that underlie their properties remain undefined. METHODS: The clinical manifestations of three CCD patients were collected and the mutations of RUNX2 were analyzed...
December 3, 2018: Journal of Cellular Biochemistry
Priyanka Kanth, Mark W Hazel, Kenneth M Boucher, Zhihong Yang, Li Wang, Mary P Bronner, Katherine Boylan, Randall W Burt, Michelle Westover, Deborah W Neklason, Don A Delker
Sessile serrated adenoma/polyps (SSA/Ps) of the colon account for 20-30% of all colon cancers. Small non-coding RNAs, including microRNAs (miRNAs), can regulate the expression of many protein-coding genes important in cancer development. Some miRNAs have been classified as oncogenes or tumor suppressor genes depending on the type of cancer. We have used small RNA sequencing (RNA-seq) to characterize miRNA profiles in SSA/Ps, hyperplastic polyps (HPs), adenomatous polyps (AP) and paired uninvolved colon mucosa to identify miRNAs specific to SSA/Ps and associated with colon cancer...
September 28, 2018: Genes, Chromosomes & Cancer
D R Dolgova, O S Voronova, T V Abakumova, T P Gening, I O Zolotovsky, L V Poludnyakova, E V Slesareva, E V Smirnova, A B Peskov
BACKGROUND: The effects of low-intensity laser radiation (LILR) on the skin depend on the wavelength and density of the irradiation flux. Moreover, the vast receptor field of the skin facilitates the systemic influence of irradiation on the body. AIMS: The objective of the present study was to evaluate the effects of low-intensity laser irradiation (LIRI) of the infrared range with a wavelength of 1270 nm on the skin of mice Balb/cNude. MATERIAL AND METHODS: The study was carried out with the use of the linear immunodeficient mice Balb/c nude obtained from the animal house of the Pushchino branch of the Academicians M...
2018: Voprosy Kurortologii, Fizioterapii, i Lechebnoĭ Fizicheskoĭ Kultury
Cristina Solé, Sandra Domingo, Berta Ferrer, Teresa Moliné, Josep Ordi-Ros, Josefina Cortés-Hernández
Cutaneous lupus erythematosus is a common and disfiguring manifestation in systemic lupus erythematosus. Subacute cutaneous lupus erythematosus and discoid lupus erythematosus (DLE) are the most prevalent forms. Despite sharing histological similarities, clinically they differ in their course and prognosis, suggesting different pathogenesis. Here, we show that DLE-affected skin has a specific microRNA expression profile when compared with subacute cutaneous lupus erythematosus. Among the DLE-specific microRNAs, we identified one keratinocyte-derived microRNA, miR-31, and one leukocyte-derived microRNA, miR-485-3p...
January 2019: Journal of Investigative Dermatology
Peng Cai, Hongxing Li, Weiwei Huo, Hairong Zhu, Chao Xu, Rujin Zang, Wei Lv, Yankai Xia, Weibing Tang
Hirschsprung's disease (HSCR) is a birth defect that causes a failure of the enteric nervous system to cover the distal gut during early embryonic development. Evidence shows that long non-coding RNAs (lncRNA) play important roles in HSCR. The MIR31 host gene (MIR31HG), also known as Loc554202, is a long non-coding RNA (lncRNA), which acts as the host gene of (microRNA) miR-31 and miR-31*. There have been no studies regarding its function in early developmental defects during pregnancy, and its downstream genetic receptors...
November 2018: Journal of Cellular Biochemistry
Ibrahim O Alanazi, Sami A AlYahya, Esmaeil Ebrahimie, Manijeh Mohammadi-Dehcheshmeh
Exponentially growing scientific knowledge in scientific publications has resulted in the emergence of a new interdisciplinary science of literature mining. In text mining, the machine reads the published literature and transfers the discovered knowledge to mathematical-like formulas. In an integrative approach in this study, we used text mining in combination with network discovery, pathway analysis, and enrichment analysis of genomic regions for better understanding of biomarkers in lung cancer. Particular attention was paid to non-coding biomarkers...
June 15, 2018: Gene
Martha L Slattery, Andrew J Pellatt, Frances Y Lee, Jennifer S Herrick, Wade S Samowitz, John R Stevens, Roger K Wolff, Lila E Mullany
Half of miRNAs expressed in colorectal tissue are expressed < 50% of the population. Many infrequently expressed miRNAs have low levels of expression. We hypothesize that less frequently expressed miRNAs, when expressed at higher levels, influence both disease stage and survival after diagnosis with colorectal cancer (CRC); low levels of expression may be background noise. We examine 304 infrequently expressed miRNAs in 1893 population-based cases of CRC with paired carcinoma and normal mucosa miRNA profiles...
October 13, 2017: Oncotarget
X Wang, Y Zhang, B H Jiang, Q Zhang, R P Zhou, L Zhang, Chen Wang
Hypertrophic scar (HS) formation is associated with the fibrosis of fibrocytes caused by excessive extracellular matrix (ECM) synthesis and deposition, the initial event of HS formation. Our high throughput screen of miRNA expression profiles identified hsa-miR31-5p, whose transcription level was most differentially in normal skin fibroblasts (NS) and HS among other miRNAs. The level of hsa-miR31-5p in HS was significantly higher than in NS. In-vitro functional experiments showed hsa-miR31-5p knockdown remarkably suppressed the proliferation of hypertrophic scar fibroblasts (HSFBs) under hypoxia, promoted cell invasion, and inhibited the expression of Collagen I and III and Fibronectin (FN), suggesting that hsa-miR31-5p knockdown effectively reduces HS formation caused by excessive ECM synthesis and deposition in HSFBs under hypoxia...
December 15, 2017: Experimental Cell Research
Cong Lv, Fengyin Li, Xiang Li, Yuhua Tian, Yue Zhang, Xiaole Sheng, Yongli Song, Qingyong Meng, Shukai Yuan, Liming Luan, Thomas Andl, Xu Feng, Baowei Jiao, Mingang Xu, Maksim V Plikus, Xing Dai, Christopher Lengner, Wei Cui, Fazheng Ren, Jianwei Shuai, Sarah E Millar, Zhengquan Yu
MicroRNA-mediated post-transcriptional regulation plays key roles in stem cell self-renewal and tumorigenesis. However, the in vivo functions of specific microRNAs in controlling mammary stem cell (MaSC) activity and breast cancer formation remain poorly understood. Here we show that miR-31 is highly expressed in MaSC-enriched mammary basal cell population and in mammary tumors, and is regulated by NF-κB signaling. We demonstrate that miR-31 promotes mammary epithelial proliferation and MaSC expansion at the expense of differentiation in vivo...
October 19, 2017: Nature Communications
Jie Wang, Ran Chen, Ruiyang Zhang, Shanlong Ding, Tianying Zhang, Quan Yuan, Guiwen Guan, Xiangmei Chen, Ting Zhang, Hui Zhuang, Frederick Nunes, Timothy Block, Shuang Liu, Zhongping Duan, Ningshao Xia, Zhongwei Xu, Fengmin Lu
The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter. With an anti-HBV pri-miR31 mimic integrated between two HBV-specific gRNAs, both gRNAs could be separated from the long transcript of gRNA-miR-HBV-gRNA ternary cassette through Drosha/DGCR8 processing. The results showed that the gRNA-miR-HBV-gRNA ternary cassette could efficiently express two gRNAs and miR-HBV...
2017: Theranostics
Laura Lorenzon, Claudia Cippitelli, Riccardo Avantifiori, Stefania Uccini, Deborah French, Maria Rosaria Torrisi, Danilo Ranieri, Paolo Mercantini, Valeria Canu, Giovanni Blandino, Marco Cavallini
BACKGROUND AND OBJECTIVES: Gastric cancers are usually characterized using Lauren's classification into intestinal and diffuse types. We previously documented the down-modulation of miR31, miR148a, miR204, and miR375 in gastric cancers. We aimed this manuscript to investigate these miRs with the end-points of diagnosis, Lauren's classification and prognosis. METHODS: A total of 117 resected non-cardial adenocarcinomas were evaluated for miRs' expressions. The performance of miRs' expressions for cancer diagnosis was tested using ROC curves...
August 2017: Journal of Surgical Oncology
Ye Zhen, Zhao Xinghui, Wu Chao, Zhao Yi, Chen Jinwen, Gao Ruifang, Zhang Chao, Zhao Min, Guo Chunlei, Fang Yan, Du Lingfang, Shen Long, Shen Wenzhi, Luo Xiaohe, Xiang Rong
MicroRNAs as biomarkers play an important role in the tumorigenesis process, including hepatocellular carcinomas (HCCs). In this paper, we used The Cancer Genome Atlas (TCGA) database to mine hepatitis B-related liver cancer microRNAs that could predict survival in patients with hepatitis B-related liver cancer. There were 93 cases of HBV-HCC and 49 cases of adjacent normal controls included in the study. Kaplan-Meier survival analysis of a liver cancer group versus a normal control group of differentially expressed genes identified eight genes with statistical significance...
March 21, 2017: Scientific Reports
Beatriz Revilla-Nuin, África de Bejar, Laura Martínez-Alarcón, José Ignacio Herrero, Carlos Manuel Martínez-Cáceres, Pablo Ramírez, Alberto Baroja-Mazo, José Antonio Pons
Regulatory T cells (Tregs) play a potential role in operational tolerance in liver transplantation (LT) patients, and microRNAs (miRNAs) are known to be involved in immunological responses and tolerance. Thus, we analyzed the implication of different peripheral blood Treg subsets and miRNAs on LT tolerance in 24 tolerant (Tol) and 23 non-tolerant (non-Tol) LT recipients by cellular, genetic, and epigenetic approximation. Non-Tol patients had a lower demethylation rate of the forkhead box P3 (FOXP3) regulatory T cell-specific demethylated region (TSDR) than Tol patients that correlated with the frequency of circulating Tregs...
July 2017: Liver Transplantation
Agata A Filip, Anna Grenda, Sylwia Popek, Dorota Koczkodaj, Małgorzata Michalak-Wojnowska, Michał Budzyński, Ewa Wąsik-Szczepanek, Szymon Zmorzyński, Agnieszka Karczmarczyk, Krzysztof Giannopoulos
Expression of microRNAs is altered in cancer. Circulating miRNA level assessed in body fluids commonly reflects their expression in tumor cells. In leukemias, however, both leukemic and nonleukemic cells compose circulating miRNA expression profile of peripheral blood. The latter contribution to extracellular miRNA pool may result in specific microenvironmental signaling, which promotes proliferation and survival. In our study, we used qT-PCR to assay peripheral blood serum of 22 chronic lymphocytic leukemia (CLL) patients for the expression of 84 miRNAs associated with activation and differentiation of B and T lymphocytes...
January 2017: Annals of Hematology
Soumitra Paul, Dipak Gayen, Swapan K Datta, Karabi Datta
The present study highlights the molecular regulation of iron transport in soyFER1-overexpressing transgenic rice. Accumulation of iron in three different seed developmental stages, milk, dough, and mature, has been examined. The transgenic seeds of the milk stage showed significant augmentation of iron and zinc levels compared with wild-type seeds, and similar results were observed throughout the dough and mature stages. To investigate the regulation of iron transport, the role of miRNAs was studied in roots of transgenic rice...
October 2016: Journal of Experimental Botany
Esita Chattopadhyay, Richa Singh, Anindita Ray, Roshni Roy, Navonil De Sarkar, Ranjan Rashmi Paul, Mousumi Pal, Ritesh Aich, Bidyut Roy
Oral cancer generally progresses from precancerous lesions such as leukoplakia (LK), lichen planus (LP) and oral submucous fibrosis (OSMF). Since few of these precancers progress to cancers; it is worth to identify biological molecules that may play important roles in progression. Here, expression deregulation of 7 miRNAs (mir204, mir31, mir31*, mir133a, mir7, mir206 and mir1293) and their possible target genes in 23 cancers, 18 LK, 12 LP, 23 OSMF tissues compared to 20 healthy tissues was determined by qPCR method...
September 6, 2016: Scientific Reports
John Cw Hildyard, Dominic J Wells
Exon-skipping via synthetic antisense oligonucleotides represents one of the most promising potential therapies for Duchenne muscular dystrophy (DMD), yet this approach is highly sequence-specific and thus each oligonucleotide is of benefit to only a subset of patients. The discovery that dystrophin mRNA is subject to translational suppression by the microRNA miR31, and that miR31 is elevated in the muscle of DMD patients, raises the possibility that the same oligonucleotide chemistries employed for exon skipping could be directed toward relieving this translational block...
2016: PLoS Currents
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