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Huirong Li, Yuanyuan Hou, Shitong Zhang, Yuanqin Zhou, Dezheng Wang, Siyue Tao, Fang Ni
PROBLEM: The function of CD49a on human decidual natural killer (dNK) cells is unknown. METHOD OF STUDY: The expression of CD49a on dNK cells from human patients with recurrent spontaneous abortions or age-matched healthy controls was analyzed by flow cytomertry. DNK cells were treated with CD49a neutralizing antibody and analyzed for function (cytokines production and cytotoxic activity). Long noncoding RNA (lncRNA) microarray analysis was used to identify a potential regulator of CD49a...
February 13, 2019: American Journal of Reproductive Immunology: AJRI
U-Ging Lo, Rey-Chen Pong, Diane Yang, Leah Gandee, Elizabeth Hernandez, Andrew Dang, Chun-Jung Lin, John Santoyo, Shihong Ma, Rajni Sonavane, Jun Huang, Shu-Fen Tseng, Loredana Moro, Arnaldo A Arbini, Payal Kapur, Ganesh V Raj, Dalin He, Chih-Ho Lai, Ho Lin, Jer-Tsong Hsieh
Interferon-γ (IFNγ), a potent cytokine known to modulate tumor immunity and tumoricidal effects, is highly elevated in prostate cancer (PCa) patients after radiation. In this study, we demonstrate that IFNγ can induce epithelial-to-mesenchymal transition (EMT) in PCa cells via the JAK-STAT signaling pathway, leading to the transcription of IFN-stimulated genes (ISG) such as interferon-induced tetratricopeptide repeat 5 (IFIT5). We unveil a new function of IFIT5 complex in degrading precursor microRNAs (pre-miRNA) that include pre-miR-363 from the miR-106a-363 cluster as well as pre-miR-101 and pre-miR-128, who share a similar 5'-end structure with pre-miR-363...
November 30, 2018: Cancer Research
Baofeng Ma, Xiaoming Liu, Zhaoxiao Yu
OBJECTIVE: To investigate the effects of high intensity focused ultrasound on liver function, tumor markers and survival rate of hepatocellular carcinoma patients. METHODS: Ninety six cases with primary liver cancer patients, consisting of 66 males and 30 females, were enrolled in this study and treated with high intensity focused ultrasound combined with stereotactic segmentation dose radiation, low frequency for 10 times, followed by analysis of KPS score of liver cancer, Child-Pugh, grading and staging of liver cancer, 3 months, 6 months, 1 year of clinical symptom remission rate, tumor markers, liver function, survival rate, as well as the change of immune related cytokines...
October 9, 2018: Cancer Biomarkers: Section A of Disease Markers
Marcos Iglesias, Anirudh Arun, Maria Chicco, Brandon Lam, C Conover Talbot, Vera Ivanova, W P A Lee, Gerald Brandacher, Giorgio Raimondi
Destruction of insulin-producing β-cells by autoreactive T lymphocytes leads to the development of type 1 diabetes. Type-I interferons (TI-IFN) and interleukin-10 (IL-10) have been connected with the pathophysiology of this disease; however, their interplay in the modulation of diabetogenic T cells remains unknown. We have discovered that TI-IFN cause a selective inhibition of IL-10 signaling in effector and regulatory T cells, altering their responses. This correlates with diabetes development in nonobese diabetic mice, where the inhibition is also spatially localized to T cells of pancreatic and mesenteric lymph nodes...
2018: Frontiers in Immunology
Tiantian Chen, Xiaoxia Hou, Yingmeng Ni, Wei Du, Huize Han, Youchao Yu, Guochao Shi
Background: Treg cells play an important role in the pathogenic progress of asthma. Objective: To address the alterations of Treg cells in asthma. Methods: Proliferation-and function-associated markers of Treg cells along with the percentage of Treg cells producing some cytokine from asthmatics and healthy subjects were analyzed by flow cytometry. Besides, the expressions of USP21 and PIM2 in Treg cells were measured by cell immunochemistry after Treg cells were sorted...
2018: Journal of Immunology Research
Wuwei Yang, Wei Wang, Bing Liu, Baorang Zhu, Jing Li, Debao Xu, Yanli Ni, Li Bai, Guangxian Liu
AIM: Thermal ablation therapy has recently emerged as a promising noninvasive treatment modality for localized solid malignancies. Except its direct tumor-cell-killing effect on local tumor tissues, the immunomodulatory effect has also long been noticed which too has substantial effect on clinical outcome, but it is complicated. Though much has been investigated and rich evidences have been achieved, the fundamental state and profile of immunomodulation by thermal ablation in cancer patients, its exact mechanism, especially the systematic mechanism, and its effect on antitumor immunity remain unclear...
January 8, 2018: Asia-Pacific Journal of Clinical Oncology
Shaojuan Wang, Xiaoli Zhou, Jing Yang
Physiological functions of villous trophoblasts are essential for normal implantation and pregnancy, which are under fine regulations. Integrin αvβ3 has been shown to mediate cellular attachment of villous trophoblasts; however, the physiological functions of integrin αvβ3 in pregnancy have not been revealed. Here, we found that suppression of integrin αvβ3 in vivo by a small molecule inhibitor, SB-273005, resulted in abnormal pregnancy in mice. Mechanistically, suppression of integrin αvβ3 in vivo broke down the tissue homeostasis of the decidua, as revealed by disorganized histology and compromised cellular proliferation in the decidua...
September 2018: Reproductive Sciences
Seung-Wook Lee, Hyun-Young Na, Mi Hyeon Seol, Mia Kim, Byung-Cheol Lee
Background: Obesity is a main cause of insulin resistance (IR), metabolic syndrome, and fatty liver diseases. This study evaluated Euphorbia kansui radix ( Euphorbia ) as a potential treatment option for obesity and obesity-induced IR in obese human and high-fat diet- (HFD-) induced obese mice. Methods: In the human study, we analyzed the body weight change of 14 patients who took a single dose of 6 g of Euphorbia powder. In the animal study, male mice were divided into three groups: normal chow, HFD, and Euphorbia (high-fat diet and 100 mg/Kg Euphorbia once per week)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Xueen Li, Bo Wang, Lintao Gu, Jie Zhang, Xiaoyan Li, Lifen Gao, Chunhong Ma, Xiaohong Liang, Xingang Li
Malignant glioma, the most common and devastating primary brain tumor, has serious effects on human health with high risk of recurrence and short survival periods. Recently, the exploitation of immunological mechanisms shed new lights for developing novel therapeutic strategies for glioma pathogenesis. Tim-3, a member of T cell immunoglobulin and mucin domain family, has been involved in multiple diseases, including tumor, by regulating the viability and function of immunocytes. In the present study, we detected Tim-3 expression on peripheral innate immunocytes from glioma patients and analyzed their correlation with clinical indices...
January 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Lin Wang, Wei-Zhi Liu, Ling-Yan Pei, Yu-Shi Ke, Jian Cui, Shu-Chun Li
Garidisan, commonly used in Mongolia to treat ulcerative colitis (UC), contains wild poppy and Artemisia frigida Willd. Clinical evidence shows that Garidisan can effectively treat UC and that recurrence is low. Thus, we evaluated the effects of Garidisan on ulcer healing quality and the regulation of immune balance in rats with experimental UC. UC was induced by immunization with TNBS and Garidisan significantly reduced DAI, CMDI, and HS. H&E staining, SEM, and VG staining showed that Garidisan repaired damaged intestinal mucosa and significantly reduced expression of ICAM-1 and CD105 in regenerated tissues of UC rats...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Wei Li, Linping Xu, Yaomei Wang, Lingdi Zhao, Daniel B Kellner, Quanli Gao
BACKGROUND: This study aims to explore the efficacy of tumor-infiltrating lymphocytes (TIL) along with interferon- α (IFN- α ) to treat stage III malignant melanoma (MM) patients in China. METHODS: Between May 2010 and October 2014, 77 patients of stage III MM who underwent surgery were collected in this study. These patients were divided into two groups: patients who received TIL + IFN- α  ± RetroNectin-activated cytokine-induced killer cells (R-CIK) in Arm 1 ( n = 27) and IFN- α  ± R-CIK in Arm 2 ( n = 50) as adjuvant therapy...
2017: Journal of Immunology Research
Giulia Finotti, Nicola Tamassia, Marco A Cassatella
Interferon lambdas (IFNλs) are recently discovered cytokines acting not only at the first line of defense against viral infections but also at the mucosal barriers. In fact, a peculiar feature of the IFNλ system is the restricted expression of the functional IFNλR, which is known to be limited to epithelial cells and discrete leukocyte subsets, including the plasmacytoid dendritic cells (pDCs). In the latter case, current data, discussed in this minireview, indicate that IFNλs positively regulate various pDC functions, including pDC expression of interferon-dependent gene (ISG) mRNAs, production of cytokines, survival, and phenotype...
2017: Frontiers in Immunology
Ana Villegas-Mendez, Patrick Strangward, Tovah N Shaw, Ivana Rajkovic, Vinko Tosevski, Ruth Forman, Werner Muller, Kevin N Couper
Experimental cerebral malaria (ECM) is a gamma interferon (IFN-γ)-dependent syndrome. However, whether IFN-γ promotes ECM through direct and synergistic targeting of multiple cell populations or by acting primarily on a specific responsive cell type is currently unknown. Here, using a panel of cell- and compartment-specific IFN-γ receptor 2 (IFN-γR2)-deficient mice, we show that IFN-γ causes ECM by signaling within both the hematopoietic and nonhematopoietic compartments. Mechanistically, hematopoietic and nonhematopoietic compartment-specific IFN-γR signaling exerts additive effects in orchestrating intracerebral inflammation, leading to the development of ECM...
November 2017: Infection and Immunity
Yukari Yagi-Yaguchi, Takefumi Yamaguchi, Kazunari Higa, Terumasa Suzuki, Hiroyuki Yazu, Naohiko Aketa, Yoshiyuki Satake, Kazuo Tsubota, Jun Shimazaki
PURPOSE: To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP). DESIGN: Prospective, interventional, consecutive case series. METHODS: This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery...
September 2017: American Journal of Ophthalmology
Ji Eun Oh, Kwang Yong Shim, Jong In Lee, Soo In Choi, Soon Koo Baik, Young Woo Eom
Liver fibrosis, a precursor to cirrhosis, is the result of the deposition of extracellular matrix (ECM) proteins and is mediated primarily by activated hepatic stellate cells (HSCs). In this study, we investigated the anti-fibrotic effects of interferon (IFN)-γ in activated HSCs in vitro and whether cell viability would be decreased by the inhibition of indoleamine 2,3-dioxygemase (IDO), which is responsible for cell cycle arrest. Following treatment with IFN-γ, cell signaling pathways and DNA content were analyzed to assess the inactivation of HSCs or the decrease in HSC proliferation...
August 2017: International Journal of Molecular Medicine
Catherine Matte-Martone, Jinling Liu, Meng Zhou, Maria Chikina, Douglas R Green, John T Harty, Warren D Shlomchik
The graft-versus-leukemia (GVL) effect in allogeneic hematopoietic stem cell transplantation (alloSCT) is potent against chronic phase chronic myelogenous leukemia (CP-CML), but blast crisis CML (BC-CML) and acute myeloid leukemias (AML) are GVL resistant. To understand GVL resistance, we studied GVL against mouse models of CP-CML, BC-CML, and AML generated by the transduction of mouse BM with fusion cDNAs derived from human leukemias. Prior work has shown that CD4+ T cell-mediated GVL against CP-CML and BC-CML required intact leukemia MHCII; however, stem cells from both leukemias were MHCII negative...
June 30, 2017: Journal of Clinical Investigation
Noriyuki Takahashi, Takayuki Saitoh, Nanami Gotoh, Yasuhiro Nitta, Lobna Alkebsi, Tetsuhiro Kasamatsu, Yusuke Minato, Akihiko Yokohama, Norifumi Tsukamoto, Hiroshi Handa, Hirokazu Murakami
BACKGROUND: T-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing...
May 16, 2017: BMC Immunology
Chen Ni, Pan Ma, Liwei Qu, Fan Wu, Junfeng Hao, Ruirui Wang, Yu Lu, Wei Yang, Ulrike Erben, Zhihai Qin
Angiostasis mediated by interferon (IFN)-γ is a key mechanism of anti-tumour immunity; however, the effect of IFN-γ on host vascular endothelial growth factor A (VEGFA)-expressing cells during tumour progression is still elusive. Here, we developed transgenic mice with IFN-γ receptor (IFNγR) expression under control of the Vegfa promoter (V-γR). In these mice, the IFN-γ responsiveness of VEGFA-expressing cells led to dramatic growth suppression of transplanted lung carcinoma cells. Surprisingly, increased mortality and tumour metastasis were observed in the tumour-bearing V-γR mice, in comparison with the control wild-type and IFNγR-deficient mice...
July 2017: Journal of Pathology
Adrian Pickar, Pei Xu, Andrew Elson, James Zengel, Christian Sauder, Steve Rubin, Biao He
Although mumps vaccines have been used for several decades, protective immune correlates have not been defined. Recently, mumps outbreaks have occurred in vaccinated populations. To better understand the causes of the outbreaks and to develop means to control outbreaks in mumps vaccine immunized populations, defining protective immune correlates will be critical. Unfortunately, no small animal model for assessing mumps immunity exists. In this study, we evaluated use of type I interferon (IFN) alpha/beta receptor knockout mice (IFN-α/βR-/-) for such a model...
2017: PloS One
Yanhua Li, Jiuping Wang, Juan Wang, Yunfeng Xiao, Bin Xu, Hongwei Li, Liu Yang, Xiaoke Hao, Yueyun Ma
BACKGROUND: Chronic HCV Patients taking PEG-IFN-α/R from different ethnic groups have different probabilities of reaching a sustained viral response (SVR). There are many influence factors, such as HCV genotype, IL-28B single-nucleotide polymorphisms (SNP), Fibrosis 4 index (FIB-4), and aspartate aminotransferase-to-platelet ratio index (APRI) score. But the baseline factors in relation to treatment outcome was still not much clear. METHODS: We evaluated data from 231 chronic HCV patients with or without liver fibrosis and their antiviral efficacy after treatment with pegylated interferon plus ribavirin (PEG-IFN-α/R) for 24-48 weeks...
March 23, 2017: Virology Journal
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