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Embryo morphokinetics

Georges Raad, Joseph Azouri, Kamal Rizk, Nina S Zeidan, Jessica Azouri, Valérie Grandjean, Mira Hazzouri
Growing evidence suggests that paternal obesity may decrease male fertility potential. During infertility treatment with intra-cytoplasmic sperm injection (ICSI), a morphologically normal motile spermatozoon is injected into a mature egg, when possible. However, sperm motility and morphology per se do not reflect the sperm molecular composition. In this study, we aimed to assess the quality of motile spermatozoa in the context of obesity by analysing their conventional and molecular characteristics as well as their ability to promote early embryonic development...
2019: PloS One
Fazilet Kubra Boynukalin, Munevver Serdarogullari, Meral Gultomruk, Onder Coban, Necati Findikli, Mustafa Bahceci
The aim of this study was to evaluate the possible effects of endometriosis on early embryo development, by comparing the morphokinetic development of embryos obtained from women with clinically confirmed endometriosis with the ones obtained from tubal factor infertility cases. A total of 82 cycles/patients including 53 cycles with endometriosis and 29 cycles with tubal factor infertility were evaluated. A total of 439 embryos were scored for embryo morphokinetics. Age, body mass index, fertilization rates were similar within the groups...
February 11, 2019: Systems Biology in Reproductive Medicine
C Scarica, D Cimadomo, L Dovere, A Giancani, M Stoppa, A Capalbo, F M Ubaldi, L Rienzi, R Canipari
PURPOSE: To investigate the association of cumulus cell (CC)-related expression of a selected cluster of key genes (PTGS2, CAMK1D, HAS2, STC1, and EFNB2) with embryo development to blastocyst. METHODS: Exploratory study at a private clinic. Eighteen advanced maternal age patients were enrolled (37.3 ± 4.0 years). Seventy-five cumuli were collected, whose oocytes resulted in either developmental arrest (N = 33) or blastocyst formation (N = 42). The noninvasive CC gene expression was combined with time-lapse morphokinetic parameters and, for blastocysts, with qPCR-based aneuploidy testing on trophectoderm biopsies...
February 1, 2019: Journal of Assisted Reproduction and Genetics
N Zaninovic, M Nohales, Q Zhan, Z M J de Los Santos, J Sierra, Z Rosenwaks, M Meseguer
PURPOSE: To demonstrate whether the standard morphokinetic markers used for embryo selection have a similar relationship to blastocyst formation and implantation in two large clinical data sets. METHODS: This is a retrospective cohort analysis striving to answer two distinct questions utilizing data sets from two large IVF clinics. Blastocysts (BL) and implanted blastocysts (I) in both clinics, IVI-Valencia (BL = 11,414, I = 479) and WMC (BL = 15,902; I = 337), were cultured in a time-lapse system (EmbryoScope, Vitrolife, Sweden)...
January 22, 2019: Journal of Assisted Reproduction and Genetics
Takuya Iwasawa, Kazumasa Takahashi, Mayumi Goto, Mibuki Anzai, Hiromitsu Shirasawa, Wataru Sato, Yukiyo Kumazawa, Yukihiro Terada
Recent studies reported morphokinetic indices for optimal selection of embryos in assisted reproductive technology (ART). The morphokinetics in blastocyst stage include the collapse and re-expansion rates after thawing. However, evaluation methods using these morphokinetics have not been established, mainly because the underlying molecular mechanisms remain unclarified. In this study, we focused on the relationship between these morphokinetic observation of the blastocyst behaviour and the number of cells constituting the blastocyst...
2019: PloS One
Nasim Tabibnejad, Mohammad Hasan Sheikhha, Nasrin Ghasemi, Farzaneh Fesahat, Mehrdad Soleimani, Abbas Aflatoonian
RESEARCH QUESTION: Can a combination of time-lapse morphokinetic parameters and cumulus cell gene expression in polycystic ovary syndrome (PCOS) women be used to predict assisted reproductive treatment outcome? DESIGN: A total of 547 embryos from 100 intracytoplasmic sperm injection (ICSI) cycles were evaluated. Fifty women with PCOS and 50 women who were categorized as tubal factor infertility were recruited. Time-lapse records were annotated for time to pronuclear fading (tPNf), time to 2 to 8 cells (t2-t8), reverse cleavage, direct cleavage and also for the presence of multinucleation...
December 14, 2018: Reproductive Biomedicine Online
Jenna Lammers, Arnaud Reignier, Carole Splingart, Kamran Moradkhani, Paul Barrière, Thomas Fréour
RESEARCH QUESTION: Can embryo morphokinetic parameters help identify unbalanced embryos in translocation carriers? DESIGN: This retrospective study was conducted in 67 translocation carriers undergoing 105 preimplantation genetic testing cycles for chromosomal structural rearrangements (PGT-SR) without aneuploidy screening (PGT-A). Using time-lapse imaging analysis, morphokinetic parameters of balanced and unbalanced embryos were compared, as well as the frequency of abnormal cellular events...
December 7, 2018: Reproductive Biomedicine Online
Ali Buran, Pinar Tulay, Nurten Dayıoğlu, Mustafa Emre Bakircioglu, Mustafa Bahceci, Tulay İrez
Low sperm quality has negative effects on fertilisation and embryo development. The males with azoospermia apply for testicular sperm extraction (TESE) or microsurgical epididymal sperm aspiration (MESA) in order to retrieve sperm. To date, there have not been any reports investigating morphokinetic parameters of pre-implantation embryos using testicular and epididymal spermatozoa. Therefore, we aimed to correlate embryo development and assess morphogenetic parameters in embryos obtained by TESE and MESA using time-lapse imaging...
December 19, 2018: Andrologia
L Escrich, Y Galiana, N Grau, F Insua, N Soler, A Pellicer, M J Escribá
RESEARCH QUESTION: How can laboratory and clinical outcomes of spontaneously, early maturing germinal-vesicle oocytes and sibling in-vivo-matured (metaphase II [MII]) oocytes be quantified and compared? DESIGN: A prospective, non-randomized intra-cohort study of oocytes from women aged 38 years or younger, with six or fewer MII oocytes and four or more germinal vesicles retrieved. No indication was identified for genetic tests or oocyte or embryo cryopreservation...
December 2018: Reproductive Biomedicine Online
Elena Labarta, Maria José de Los Santos, Sonia Herraiz, Maria José Escribá, Alicia Marzal, Anna Buigues, Antonio Pellicer
OBJECTIVE: To study if autologous mitochondrial transfer (AUGMENT) improves outcome in patients with previously failed in vitro fertilization (IVF). DESIGN: Randomized, controlled, triple-blind, experimental study. SETTING: Private infertility center, Valencian Institute of Infertility (IVI-RMA), Valencia, Spain. PATIENT(S): Infertile women ≤42 years of age, body mass index <30 kg/m2 , antimüllerian hormone ≥4 pmol/L, >5 million/mL motile sperm, at least one previous IVF with at least five metaphase oocytes (MIIs) collected, and low embryo quality...
November 24, 2018: Fertility and Sterility
Tamar Margalit, Avi Ben-Haroush, Roni Garor, Naomi Kotler, Dania Shefer, Natalia Krasilnikov, Moran Tzabari, Galia Oron, Yoel Shufaro, Onit Sapir
RESEARCH QUESTION: Does delayed maturation of aspirated metaphase I (MI) oocytes, completed in vitro, adversely affect early embryo development? DESIGN: Time-lapse microscopy was used to compare morphokinetic variables between embryos derived from oocytes with delayed maturation after ovarian stimulation and from in-vivo-matured metaphase II (MII) sibling oocytes from the same IVF and intracytoplasmic sperm injection cycle. RESULTS: A total of 1545 injected oocytes in 169 cycles from 149 patients were included...
November 10, 2018: Reproductive Biomedicine Online
Bo Huang, Xinling Ren, Lixia Zhu, Li Wu, Huiping Tan, Na Guo, Yulan Wei, Juan Hu, Qun Liu, Wen Chen, Jing Liu, Dan Li, Shujie Liao, Lei Jin
To date, very few studies have reported on the relationship between live birth gender and embryo development kinetics. This study included 1,735 women undergoing IVF or ICSI by using a time-lapse system. Finally, a total of 228 qualified patients with 100% implantation and known live birth information were included in the analysis. There were 174 male live births and 134 female live births. The time to 3 (t3), 4 (t4) and 5 (t5) cell development of male embryos was significantly shorter/earlier than female embryos (P < 0...
October 26, 2018: Biology of Reproduction
Ali Zeyad, Bandar Al-Abdulkareem, Mohamad Eid Hammadeh
The aim of this study was to determine the relationship between the gender of human embryos and chronological changes during the two pronuclear (2PN) embryonic stage and blastocyst formation on day five after injection using time-lapse imaging and preimplantation genetic testing. A total of 120 couples who underwent intracytoplasmic sperm injection with preimplantation genetic testing were included in the study. Only normal embryos (n = 416) were enrolled in this study. Time-lapse imaging data of male (n = 227) and female (n = 189) embryo progression was carried out to estimate the times of initial appearance of the 2PN stage, fading times and day five blastulation rates...
October 18, 2018: Reproductive Biology
Simon Fishel, Alison Campbell, Sue Montgomery, Rachel Smith, Lynne Nice, Samantha Duffy, Lucy Jenner, Kathryn Berrisford, Louise Kellam, Rob Smith, Fiona Foad, Ashley Beccles
RESEARCH QUESTION: Can blastocysts leading to live births be ranked according to morphokinetic-based algorithms? DESIGN: Retrospective analysis of 781 single blastocyst embryo transfers, including all patient clinical factors that might be potential confounders for the primary outcome measure of live birth, was weighed using separate multi-variable logistic regression models. RESULTS: There was strong evidence of effect of embryo rank on odds of live birth...
September 2018: Reproductive Biomedicine Online
Gláucia Pereira Alves, Fernanda Bertuccez Cordeiro, Camila Bruna de Lima, Kelly Annes, Érika Cristina Dos Santos, Jéssica Ispada, Patrícia Kubo Fontes, Marcelo Fabio Gouveia Nogueira, Marcílio Nichi, Marcella Pecora Milazzotto
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL)...
October 10, 2018: Reproduction, Fertility, and Development
Nasim Tabibnejad, Mehrdad Soleimani, Abbas Aflatoonian
Background: Anti-Mullerian hormone (AMH) is considered as a good marker for quantitative evaluation of ovarian response to the stimulation during assisted reproductive technology cycles. Objective: To evaluate the association between serum AMH level and embryo morphokinetics using time-lapse imaging and intracytoplasmic sperm injection (ICSI) outcomes in women with polycystic ovarian syndrome (PCOS). Materials and Methods: We evaluated a total of 547 embryos from 100 women underwent ICSI cycles; 50 women with PCOS and 50 women with tubal factor infertility...
August 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
M Esbert, A Pacheco, S R Soares, D Amorós, M Florensa, A Ballesteros, M Meseguer
BACKGROUND: Time-lapse monitoring (TLM) technology has been implemented in the clinical setting for the culture and selection of human embryos. Many studies have assessed the association between sperm DNA fragmentation (sDNAf) and clinical outcomes after ART, but little is known about the influence of sDNA on embryo morphokinetics. OBJECTIVES: The objective of this retrospective study, which includes 971 embryos from 135 consecutive ICSI cycles (56 cases with own oocytes, 79 with oocytes from young and healthy donors), was to assess if sDNAf has an impact on embryo morphokinetics...
September 26, 2018: Andrology
N Tabibnejad, M Soleimani, A Aflatoonian
OBJECTIVES: Oocyte parameters as well as embryo developmental kinetics can be applied as non-invasive predictors for selection of viable embryos and clinical outcomes. Time-lapse morphokinetic events in conjunction with zona pellucida birefringence (ZPB) and meiotic spindle visualization (MSV) have been evaluated for predicting pregnancy outcome. STUDY DESIGN: We assessed a total of 547 embryos from 100 intra cytoplasmic sperm injection cycles in women with polycystic ovary syndrome (PCOS) and tubal factor infertility...
November 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
S Lahav-Baratz, I Blais, M Koifman, D Ishai, Z Wiener-Megnazi, G Peer, M Dirnfeld
We report a live birth from a heavily fragmented embryo which continued cleavage to a fully expanded blastocyst. A 32-year-old patient underwent 2 IVF cycles without achieving pregnancy. In the first cycle, 2 embryos with fragmentation were transferred; in the second, all embryos were fragmented and no embryo transfer was performed. In a third cycle, 12 oocytes were retrieved and 11 of them were fertilized. On day 2, all 11 embryos started to unwind to fragments. By careful annotation, using the time-lapse EmbryoScope, we observed that one embryo continued division as expected, discarding all fragments aside...
2018: Case Reports in Obstetrics and Gynecology
Quyen Nguyen, Sophia Sommer, Brandon Greene, Christine Wrenzycki, Uwe Wagner, Volker Ziller
OBJECTIVE: To evaluate the effects of incubator door openings by the sensitive measurement of morphokinetics using time-lapse imaging. STUDY DESIGN: To mimic lab practice and to evaluate minimal changes in culture conditions a randomized parallel group study with mouse embryos was designed. 249 murine zygotes derived from 31 mice were randomly distributed into two groups. Embryos of the control group were non-invasively monitored with a Primovision time-lapse system (Vitrolife) in the incubator that was completely closed for 5 days, while the conditions for the embryos in the treatment group were interrupted by two door openings per day of 10 s each...
October 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
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