Henna Konttinen, Mauricio E Castro Cabral-da-Silva, Sohvi Ohtonen, Sara Wojciechowski, Anastasia Shakirzyanova, Simone Caligola, Rosalba Giugno, Yevheniia Ishchenko, Damián Hernández, Mohammad Feroze Fazaludeen, Shaila Eamen, Mireia Gómez Budia, Ilkka Fagerlund, Flavia Scoyni, Paula Korhonen, Nadine Huber, Annakaisa Haapasalo, Alex W Hewitt, James Vickers, Grady C Smith, Minna Oksanen, Caroline Graff, Katja M Kanninen, Sarka Lehtonen, Nicholas Propson, Michael P Schwartz, Alice Pébay, Jari Koistinaho, Lezanne Ooi, Tarja Malm
Here we elucidate the effect of Alzheimer disease (AD)-predisposing genetic backgrounds, APOE4, PSEN1ΔE9, and APPswe, on functionality of human microglia-like cells (iMGLs). We present a physiologically relevant high-yield protocol for producing iMGLs from induced pluripotent stem cells. Differentiation is directed with small molecules through primitive erythromyeloid progenitors to re-create microglial ontogeny from yolk sac. The iMGLs express microglial signature genes and respond to ADP with intracellular Ca2+ release distinguishing them from macrophages...
September 4, 2019: Stem Cell Reports