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Molecular test

Poliana de Oliveira Figueiredo, Danilo Bretas de Oliveira, Leandra Barcelos Figueiredo, Galileu Barbosa Costa, Pedro Augusto Alves, Maria Isabel Maldonado Coelho Guedes, Edel Figueiredo Barbosa-Stancioli, Betânia Paiva Drumond, Jônatas Santos Abrahão, Erna Geessien Kroon, Giliane de Souza Trindade
We examined the circulating BVDV species and genotypes among cattle herds from Northeast, Southeast, and Midwest regions in Brazil. A total of 77 animals tested positive through standard PCR. Phylogenetic analyses revealed the presence of BVDV-1a, highlighting the need for better surveillance strategies to prevent BVDV spread in the country.
March 16, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Mathias V Teye, Teresa K Sebunya, Elliot M Fana, Donald P King, LaToya Seoke, Nick J Knowles, Joseph A Awuni, George Matlho, Melvin Leteane, Joseph M K Hyera
Foot-and-mouth disease (FMD) is considered to be endemic in Ghana. However, our knowledge of the local epidemiology of the disease is restricted by a lack of serological information and data for characterized viruses causing field outbreaks. In order to improve our understanding of the prevailing situation, this study was initiated to establish the FMD viruses (FMDV) circulating in the country. During 2016, sera (n = 93) and epithelia/oral swab (n = 20) samples were collected from cattle from four districts in Southern Ghana that experienced FMD outbreaks...
March 16, 2019: Tropical Animal Health and Production
Wei Zhao, Long He, Tian-Le Xiang, Ya-Jie Tang
The colchicine binding site of tubulin is an attractive molecular target domain for cancer therapies. However, there was no FDA approved drug for targeting colchicine domain. Our previous crystallography discovered that a potential binding site of αT5 loop-αH7 nearby colchicine domain was beneficial for introducing affinity fragment. In this work, benzo heterocycles (i.e., indole, indazole and quinoline) with the high affinity ability of αT5 loop-αH7 were chosen as affinity fragment to modify the molecule structure of podophyllotoxin for improving the tubulin binding affinity...
March 8, 2019: European Journal of Medicinal Chemistry
Chi Nguyen, Gillian Murray, Sarah Anderson, Allan Filipowicz, Krista K Ingram
BACKGROUND: Young adults are disproportionately affected by depression and related mental disorders. Circadian misalignment (a phase advance or delay in the body's internal clock timing) is thought to exert adverse effects on downstream physiological processes regulating mood. Circadian disruption may represent an additional, under-appreciated risk factor affecting young adults. Here, we test the hypothesis that depression in young adults is associated with circadian misalignment-the lack of concordance between an individual's endogenous rhythm and their external social and academic environment...
March 8, 2019: Journal of Affective Disorders
Neus Roca-Ayats, Núria Martínez-Gil, Mónica Cozar, Marina Gerousi, Natàlia Garcia-Giralt, Diana Ovejero, Leonardo Mellibovsky, Xavier Nogués, Adolfo Díez-Pérez, Daniel Grinberg, Susanna Balcells
Genome-wide association studies (GWAS) have repeatedly identified genetic variants associated with bone mineral density (BMD) and osteoporotic fracture in non-coding regions of C7ORF76, a poorly studied gene of unknown function. The aim of the present study was to elucidate the causality and molecular mechanisms underlying the association. We re-sequenced the genomic region in two extreme BMD groups from the BARCOS cohort of postmenopausal women to search for functionally relevant variants. Eight selected variants were tested for association in the complete cohort and 2 of them (rs4342521 and rs10085588) were found significantly associated with lumbar spine BMD and nominally associated with osteoporotic fracture...
March 13, 2019: Bone
Ekaterina Subbotina, Hua-Qian Yang, Ivan Gando, Nori Williams, Barbara A Sampson, Yingying Tang, William A Coetzee
BACKGROUND: Genetic variation in ion channel genes ('channelopathies') are often associated with inherited arrhythmias and sudden death. Genetic testing ('molecular autopsies') of channelopathy genes can be used to assist in determining the likely causes of sudden unexpected death. However, different in silico approaches can yield conflicting pathogenicity predictions and assessing their impact on ion channel function can assist in this regard. METHODS AND RESULTS: We performed genetic testing of cases of sudden expected death in the New York City metropolitan area and found four rare or novel variants in ABCC9, which codes for the regulatory SUR2 subunit of KATP channels...
February 27, 2019: Forensic Science International
Luis Piñeiro, Juan Carlos Galán, Martí Vall-Mayans
Sexually transmitted infections caused by Chlamydia trachomatis, including lymphogranuloma venereum and Mycoplasma genitalium have increased in last decade. This epidemiological scenario presents new challenges in order to improve and strengthen our control and prevention strategies. The routine clinical diagnosis of urethritis and cervicitis must be combined with the active search for the causal agent in men with symptoms of dysuria or proctitis, and in women with pelvic inflammatory disease. We should also include sexually transmitted infections screening in asymptomatic patients with sexual risk behaviours or sexual contact with patients diagnosed with an sexually transmitted infection...
March 13, 2019: Enfermedades Infecciosas y Microbiología Clínica
Fabio Tatti, Marco Petrangeli Papini, Vincenzo Torretta, Giuseppe Mancini, Maria Rosaria Boni, Paolo Viotti
Contaminants removal stoked inside low permeability zones of aquifers is one of the most important challenge of groundwater remediation process today. Low permeability layers can be considered persistent secondary sources of contamination because they release pollutants by molecular diffusion after primary source of contamination is reduced, causing long plum tails (Back-Diffusion). In this study, the Groundwater Circulation Well (GCW) system was investigated as an alternative remediation technology to the low efficient traditional pumping technologies to restore contaminated low permeability layers of aquifers...
March 9, 2019: Journal of Contaminant Hydrology
Vanessa Petry do Canto, Claudia Elizabeth Thompson, Paulo Augusto Netz
The global production of plastics increases every year, because these materials are widely used in several segments of modern life. Polyurethanes are a very important class of polymers, used in many areas of everyday life, from automotive equipments to mattresses. The waste management usually involves accumulation in landfills, incineration, and reuse processes. However, bioremediation processes are being increasingly tested, due to the efficiency of enzymes in the degradation, besides adding value to the generated waste...
March 6, 2019: Journal of Molecular Graphics & Modelling
Cui-Bing Bai, Hai-Yun Fan, Rui Qiao, Sheng-Nan Wang, Biao Wei, Qian Meng, Zi-Qian Wang, Jia-Xin Liao, Jie Zhang, Lin Zhang, Shui-Sheng Chen, Hui Miao
A methionine methyl ester-modified coumarin derivative was designed and synthesized, which could discriminate Cu2+ from other metal ions in HEPES buffer (10 mM, pH 7.4)/CH3 CN (40:60, V/V). The detection limit of WM toward Cu2+ was 1.84 × 10-7  M, which was lower than the concentration of Cu2+ in drinking water suggested by WHO and EPA. And the proposed coordination mode exhibiting the interaction between WM and Cu2+ was studied by UV-Vis, fluorescence spectrum, ESI-MS and FT-IR. Based on the fluorescent reversibility of WM, WM was considered as a molecular logic gate and molecular keypad lock...
March 8, 2019: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Sara Abdelfatah, Edmond Fleischer, Anette Klinger, Vincent Kam Wai Wong, Thomas Efferth
PLK1 has an important role in the regulation of cell cycle and represents an important target for cancer treatment. This enzyme belongs to the Polo-like kinases family, which is characterized by a regulatory domain named Polo-box domain (PBD). Rather than regular kinase inhibitors, this domain provides high selectivity to PLK1. Here, we report on four novel PLK1 PBD inhibitors identified by cytotoxicity screening and fluorescence polarization assay of a chemical library of natural and semisynthetic compounds...
March 15, 2019: Investigational New Drugs
Sinchita Roy-Chowdhuri, Pasquale Pisapia, Manuel Salto-Tellez, Spasenija Savic, Mariantonia Nacchio, Dario de Biase, Giovanni Tallini, Giancarlo Troncone, Fernando Schmitt
In recent years, cytopathology has established itself as an independent diagnostic modality to guide clinical management in many different settings. The application of molecular techniques to cytological samples to identify prognostic and predictive biomarkers has played a crucial role in achieving this goal. While earlier studies have demonstrated that single biomarker testing is feasible on cytological samples, currently, this provides only limited and increasingly insufficient information in an era where an increasing number of biomarkers are required to guide patient care...
March 15, 2019: Virchows Archiv: An International Journal of Pathology
Roser Urreizti, Klaus Mayer, Gilad D Evrony, Edith Said, Laura Castilla-Vallmanya, Neal A L Cody, Guillem Plasencia, Bruce D Gelb, Daniel Grinberg, Ulrich Brinkmann, Bryn D Webb, Susanna Balcells
DPH1variants have been associated with an ultra-rare and severe neurodevelopmental disorder, mainly characterized by variable developmental delay, short stature, dysmorphic features, and sparse hair. We have identified four new patients (from two different families) carrying novel variants in DPH1, enriching the clinical delineation of the DPH1 syndrome. Using a diphtheria toxin ADP-ribosylation assay, we have analyzed the activity of seven identified variants and demonstrated compromised function for five of them [p...
March 15, 2019: European Journal of Human Genetics: EJHG
Joanna A Pulit-Penaloza, Jessica A Belser, Terrence M Tumpey, Taronna R Maines
The relative importance of influenza virus transmission via aerosols is not fully understood, but experimental data suggests that aerosol transmission may represent a critical mode of influenza virus spread among humans. Decades ago, prototypical laboratory strains of influenza were shown to persist in aerosols; however, there is a paucity of data available covering currently circulating influenza viruses, which differ significantly from their predecessors. In this study, we evaluated the longevity of influenza viruses in aerosols generated in the laboratory...
March 15, 2019: Applied and Environmental Microbiology
Hongxiang Li, Fan Yu, Xiaoyuan Sun, Lijia Xu, Jianhua Miu, Peigen Xiao
Dihydromyricetin (DHM), the major bioactive flavonoid ingredient extracted from the leaves of Ampelopsis grossedentata (Hand.-Mazz) W.T. Wang displays multiple pharmacological activities, including oxidation resistance, anti-tumour properties and free radical scavenging capacities. However, the role of DHM in sleep deprivation (SD)-induced memory impairments and its underlying molecular mechanisms are unclear. The aim of the present study was to evaluate the effects of DHM on oxidative stress and its role in ameliorating memory impairment induced by acute SD...
March 12, 2019: European Journal of Pharmacology
Jie Wang, Lanyu Shen, Hong Hong, Jie Li, Hongtai Wang, Xiuzhen Li
Diabetic nephropathy (DN) is the most common complication of diabetes mellitus. Atrasentan (Atr) has potential therapeutic values for DN. MicroRNAs (miRNAs) function as vital regulators in the pathophysiology of kidney diseases including DN. Our present study aimed to further explore whether Atr could alleviate kidney injury by regulating microRNA-21(miR-21)/forkhead box O1 (FOXO1) in DN mouse models and cell models. Blood glucose concentration and ACR ratio were determined by matching commercial kits. MiR-21 and FOXO1 mRNA level was measured by RT-qPCR assay...
March 12, 2019: European Journal of Pharmacology
Kayleigh M Hansford, Daniel Carter, Emma L Gillingham, Luis M Hernandez-Triana, John Chamberlain, Benjamin Cull, Liz McGinley, L Paul Phipps, Jolyon M Medlock
During September 2018, a tick was submitted to Public Health England's Tick Surveillance Scheme for identification. The tick was sent from a veterinarian who removed it from a horse in Dorset, England, with no history of overseas travel. The tick was identified as a male Hyalomma rufipes using morphological and molecular methods and then tested for a range of tick-borne pathogens including; Alkhurma virus, Anaplasma, Babesia, Bhanja virus, Crimean-Congo Haemorrhagic fever virus, Rickettsia and Theileria. The tick tested positive for Rickettsia aeschlimannii, a spotted fever group rickettsia linked to a number of human cases in Africa and Europe...
March 9, 2019: Ticks and Tick-borne Diseases
João B V Campos, Marcos R André, Luiz R Gonçalves, Carla R Freschi, Filipe M Santos, Carina E de Oliveira, Eliane M Piranda, Gisele B de Andrade, Gabriel C Macedo, Rosangela Z Machado, Heitor M Herrera
Brazilian Pantanal is the world´s largest wetland ecosystem, where cattle's ranching is the most important economic activity. The objective of this study was to compile some epidemiological features on equine piroplasmids from the Nhecolândia sub-region of Pantanal wetland through the evaluation of the patterns of T. equi and B. caballi infections in different groups of horses; identification of the tick species that infest horses; and to study phylogenetic relationships among Theileria equi 18S rRNA gene sequences...
March 8, 2019: Ticks and Tick-borne Diseases
Xiuhuan Ji, Nanying Che, Rixu Lin, Jianou Chen, Xiuling Wu
In the era of personalized medicine, lung cancer is a typical disease which can be treated strategically based on the patient's histological and molecular diagnosis. Immunohistochemistry (IHC), fluorescence in-situ hybridization (FISH), Sanger sequencing and real-time PCR are techniques commonly used in clinical laboratories. Many patients are required to use several of the above technologies to get a complete diagnosis, which is expensive and timeconsuming. Next generation of sequencing (NGS) has the advantage to simultaneously analyze multigene mutations...
February 27, 2019: Pathology, Research and Practice
Esther Hernández, Visitación de Castro, Ana Avellón, Itxaso González, Nerea Muniozguren, Sonia Vázquez, Milagros Muñoz-Chimeno
INTRODUCTION: The reporting of one case of hepatitis A in a food handler at a bakery and five cases in employees of a company after consuming products from the same bakery prompted an outbreak investigation. METHODS: Outbreak cases were defined as individuals with laboratory-confirmed hepatitis A (HAV) infection, with symptoms which started in June and who, during the incubation period, worked with the food handler and/or had close contact with him and/or consumed products from the bakery...
March 12, 2019: Enfermedades Infecciosas y Microbiología Clínica
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